+

WO2009077970A1 - Procédé d'obtention d'extraits aqueux contenant des caroténoïdes et extraits obtenus - Google Patents

Procédé d'obtention d'extraits aqueux contenant des caroténoïdes et extraits obtenus Download PDF

Info

Publication number
WO2009077970A1
WO2009077970A1 PCT/IB2008/055306 IB2008055306W WO2009077970A1 WO 2009077970 A1 WO2009077970 A1 WO 2009077970A1 IB 2008055306 W IB2008055306 W IB 2008055306W WO 2009077970 A1 WO2009077970 A1 WO 2009077970A1
Authority
WO
WIPO (PCT)
Prior art keywords
products
process according
extract
skins
lycopene
Prior art date
Application number
PCT/IB2008/055306
Other languages
English (en)
Inventor
Elena Ansaloni
Francesco Carloni
Alessandro Filippini
Leonardo Setti
Dario Zanichelli
Original Assignee
Alma Mater Studiorum-Università Di Bologna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alma Mater Studiorum-Università Di Bologna filed Critical Alma Mater Studiorum-Università Di Bologna
Publication of WO2009077970A1 publication Critical patent/WO2009077970A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40—Colouring or decolouring of foods
    • A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed

Definitions

  • the present invention relates to a process to obtain aqueous extracts containing carotenoids and to the obtained extracts.
  • the agri-food sector is one of the productive fields that produces the largest amount of special wastes, for the most part non hazardous.
  • European level farming by-products have a significant potential for the development of bio-energetic industry and are estimated being as much as 250 million ton/year.
  • the management and the disposal of these by-products is a complex issue that has to be faced at social, economical and environmental level, aiming to rationally and sustainably use these resources .
  • Tomato ⁇ Lycopersicum esculentum a Solanaceae plant of the dicotyledonous family, is one of the most relevant production of the Italian agri-food sector.
  • ISTAT a total production of more than 59 million of quintal
  • a 30% is for fresh consumption while the other 70% is for food processing industry.
  • the main products obtained by tomato processing are peeled tomatoes, concentrated tomato paste, tomato puree and several variety of tomato sauces.
  • the by-product is essentially constituted by the fibrous parts of the fruit, seeds and skins.
  • the traditional use of byproducts obtained by tomato industrial transformations is the animal feeding in the zootechnic sector; in some cases these are used for thermo-valorisation purposes.
  • Lycopene a terpene of the carotenoid family, is the main antioxidant molecule contained in tomato and its content is estimated in 50-600 ⁇ g/g in each tomato skin.
  • Lycopene is the main carotenoid in human body; this is essentially due to the high amount of tomatoes consumed with the diet.
  • lycopene The antioxidant properties of lycopene allow to use this molecule not only in the pharmaceutical sector, but also as food supplement in new food products (nutraceuticals) or in the cosmetic sector to neutralize free radicals responsible of skin aging.
  • the oxidative reactions of human metabolism are responsible of the production of free radicals that have to be balanced with several molecules defined as antioxidants. This increasing interest in using lycopene attracted the attention on new methods of production and extraction of this molecule.
  • lycopene production has a low cost but the final product is contaminated by residues of toxic-hazardous organic solvents.
  • the second process in addition to the issue related to the solvents used for the extraction, there is also the need of long and expensive purification costs in order to separate other molecules (carotenoids, xantophyllins) , extracted in a non selective way that lead to a low purity grade product.
  • the vegetal material used for the extraction of lycopene become a special waste that needs to be properly disposed and will not be reused in any way, with a considerable increase of the overall process costs.
  • Supercritical CO 2 replaces traditional solvents on an industrial scale.
  • the process that use this solvent has some advantages i.e. good extraction performances by CO 2 in the supercritical phase of non-polar compounds, non toxicity of this substance, straightforward availability, non hazardousness, short time of extraction. There are anyway some non negligible drawbacks such as reaching and keeping the supercritical phase of CO 2 . Considering the high pressure needed there are also relevant problem related to the high industrial costs and safety of the process.
  • the central point of this invention is the use of surfactants that allows to pseudo-solubilise lycopene in water phase and to extract with relevant yields. This direct use on vegetal matrixes is not described in the state of the art.
  • a process to obtain an aqueous extract containing carotenoids, in particular lycopene comprising the following steps: a) mixing of plant material and, or fibrous plant byproducts containing carotenoids with an amount of water from 1 to 10 times the weight of said materials and, or by-products until a mixture is obtained; b) adding said mexture of an enzymatic catalytic system characterized by the fact that after or during step b) a surfactant is added in order to obtain an aqueous extract containing carotenoids.
  • examples of carotenoids are lycopene and, or ⁇ -carotene and as plant materials and, or fibrous by-products are advantageously used whole tomatoes and, or carrots and, or fibrous by-products of the agri-food transformation industries.
  • fibrous by-products tomato skins, solid cellulosic residues derived from peeled tomatoes and puree productions can be cited. It is possible to use both fresh and cryoconserved skins, generally at temperature of - 2O 0 C, defrosted and processed within 24 hours from de-icing.
  • thermal water that is to say spring water characterised by chemico-physical properties and peculiar inorganic salts due to the specific geochemical properties of the lands surrounding the spring.
  • the enzymatic biocatalytic system, used in the step b) of the present invention at T 20 - 50 0 C and pH 3 - 6 comprises enzymes chosen from the class formed by cellulases, pectinases, xylanases, amylases, proteases, cutinases and arylesterases .
  • step b) In order to accelerate the reaction before than step b) it is possible to perform a thermal and, or mechanical treatment of the mixture referred to in step a) .
  • mechanical treatment homogenisation and whipping can be cited.
  • the surfactants used, acting in aqueous solution are .part of the class of food-grade, natural and, or synthetic surfactants.
  • As favourites surfactants can be cited surfactants belonging to the classe formed by polysorbates, lecitins, saponins, alkyl-glucosides, sodium dodecyl sulphate, cetylstearilic alcohols, betaines, imidazolines, sarcosinates, sulphosuccinates, quaternium and polyquaternium.
  • food grade surfactants are used, among these can be cited Tween
  • the aqueous extract undergoes a filtration process to separate particles larger than 100 ⁇ m, as the smaller particles of plant material and, or by-products still contain significant amounts of lycopene; the main uses of lycopene allow to use this extract.
  • the extract obtained by the process according to the present invention undergoes a pasteurisation process, advantageously at T 90 0 C for 20-200 minutes, and hot- packed and, or added with stabilizers chosen among those generally used in the relevant technological sector.
  • the use of enzymatic catalytic systems gives several advantages.
  • the enzymatic products, as well as the surfactants, are water soluble so the entire process can be performed in water phase.
  • an extraction performed using non-hazardous enzymes and surfactants can be carried out by non specifically trained operators, i.e. in a farm, as no toxic-hazardous substance is used.
  • the enzymes contribute to make the recovery of chemicals more efficient as, hydrolyzing in a selective way plant material, the enzymes promote the release and the pseudo-solubilisation of these chemicals.
  • the process of the invention moreover, the overall cost of the lycopene extraction process is reduced if compared to similar processes. Furthermore in this way, the solid by-products of this process after contact only with water, enzymes (considered safe as already used for food applications) and surfactants (food grade or natural) are not considered as special wastes. This allows both a disposal in landfill and their reuse of the production of bio-polymers or dried products used in agriculture as fertilizer or for animal consumption or as bio-fuels, allowing to recover part of the energy used during the productive processes.
  • the agri-food by-products are generally used in fertiliser production, for animal consumption, to produce biogas, in the biological depuration and thermo-valorisation processes. Just a small fraction of these by-products nowadays is valorised by the recovery of phyto-molecules through extraction processes.
  • the extracts obtainable through the process of the invention can be used in nutraceutical, pharmaceutical and cosmetic sector.
  • the extracts of the invention can be used to produce creams, ointments, lotions, emulsions, gels, operculum, medical devices, drops, eventually added to other pharmaceutical principles .
  • Example 1 Production of an extract containing lycopene for food applications.
  • aqueous extract containing pseudo-solubilised lycopene from tomato skins for food applications the transformation of the raw material . within 12 hours from the production is preferred.
  • the skins are re-suspended in tap water or deionised water in a proportion equal to 2 folds the weight of skins to be treated.
  • the water re-suspended skins are treated at a temperature of 75°C for 90 minutes.
  • the pre-treated skins are cooled down to 3O 0 C and undergo a biocatalytic process for 4 hours at a constant temperature of 30 0 C with food grade enzymatic products having cellulasic and pectinasic activities and at least one of the following other activities: xylanasic, amylasic, proteasic, cutinasic and aryl-esterasic.
  • the hydrolysed skins are added with a food grade surfactant (i.e. Tween 20/60/80) in concentration of 5% of the skin weight at a temperature of 30 0 C for 4 hour.
  • a food grade surfactant i.e. Tween 20/60/80
  • the raw extract undergoes to several filtration treatments (using bag, cardboard and cartridge technologies) .
  • the filtrate extract is added with suitable preservatives (potassium sorbate 0.3- 2 % and/or citric acid 0.1-5% w/v and/or lactic acid 0.1- 6%) and pasteurised at 9O 0 C for 30 minutes and hot-packed in suitable containers (bag-in-boxes, tanks and cisterns among others) .
  • aqueous extract containing pseudo-solubilised lycopene from tomato skins for cosmetic applications, fresh tomato skins or stored at - 20 0 C and treated within 24 hours from the de-icing can be used.
  • the skins are re-suspended in tap water or deionised water in a proportion equal to 5 folds the weight of skins to be treated.
  • the water re-suspended skins undergo whipping processes and are treated at a temperature of 90 0 C for 10 minutes.
  • the pre-treated skins are cooled down to room temperature and undergo a biocatalytic process for 12 hours at room temperature with enzymatic products having cellulasic and pectinasic activities and at least one of the following other activities: xylanasic, amylasic, proteasic, cutinasic and aryl-esterasic.
  • the hydrolysed skins are added with a cosmetic grade surfactant, i.e. sodium dodecyl sulfate in concentration of 1% of the skin weight at room temperature for 8 hour.
  • a cosmetic grade surfactant i.e. sodium dodecyl sulfate in concentration of 1% of the skin weight at room temperature for 8 hour.
  • the raw extract undergoes several filtration treatments (using bag, cardboard and cartridge technologies) .
  • the filtrate extract is added with suitable stabilizers (m- parabenzoate 0.01-1 % w/v and/or citric acid 0.1-5% w/v and/or EDTA 0.1-3% w/v and/or phenoxyethanol 2-5 % w/v) and packed in suitable containers (bag-in-boxes, tanks and cisterns among others) .
  • aqueous extract containing pseudo-solubilised lycopene from tomato skins for cosmetic applications fresh tomato skins or stored at -20 0 C and treated within 24 hours from the de-icing can be used.
  • the skins are re-suspended in tap water or deionised water in a proportion equal to 3 times the weight of skins to be treated.
  • the water re-suspended skins are treated at a temperature of 90 0 C for 20 minutes.
  • the skins are added with a natural surfactant, such as natural saponins, natural alkyl glucosides or grounded Sapindus mukorossi nut in concentration of 3% of the skin weight at a temperature of 60 0 C for 6 hour.
  • the raw extract undergoes several filtration treatments (using bag, cardboard, cartridge and microfiltration technologies) .
  • the filtrate extract is added with suitable preservatives (bitter orange extract 0.01-1 % w/v and/or citric acid 0.1-5% w/v lactic acid 0.1-6%w/v) and packed in suitable containers (bag-in-boxes, tanks and cisterns among others) .
  • aqueous extract containing pseudo-solubilised ⁇ -carotene from carrots for food applications the transformation of fresh raw material is preferred.
  • the skins are re-suspended in tap water or deionised water in a proportion equal to 3 folds the weight of skins to be treated.
  • the water re-suspended carrots are treated at a temperature of 85°C for 90 minutes.
  • the pre-treated skins are cooled down to 30 0 C and undergo a biocatalytic process for 4 hours at a constant temperature of 30 °C with food grade enzymatic products having cellulasic and amylasic activities and at least one of the following other activities: xylanasic, pectinasic, proteasic, cutinasic and aryl-esterasic.
  • the hydrolysed carrots are added with a food grade surfactant (i.e. tween 20/60/80) in concentration of 3% of the skin weight at a temperature of 30 °C for 4 hour.
  • a food grade surfactant i.e. tween 20/60/80
  • the raw extract undergoes several filtration treatment (using bag, cardboard and cartridge technologies) .
  • the filtrate extract is added with suitable preservatives (potassium sorbate 0.3-2 % and/or citric acid 0.1-5% w/v and/or lactic acid 0.1-6%) and pasteurised at 90 0 C for 30 minutes and hot-packed in suitable containers (bag-in- boxes, tanks and cisterns among others) .

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cosmetics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

La présente invention porte sur un procédé d'obtention d'extraits aqueux contenant des caroténoïdes et sur les extraits obtenus.
PCT/IB2008/055306 2007-12-17 2008-12-15 Procédé d'obtention d'extraits aqueux contenant des caroténoïdes et extraits obtenus WO2009077970A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2007A000651 2007-12-17
IT000651A ITRM20070651A1 (it) 2007-12-17 2007-12-17 Procedimento per ottenere estratti acquosi a base di carotenoidi e estratti ottenuti.

Publications (1)

Publication Number Publication Date
WO2009077970A1 true WO2009077970A1 (fr) 2009-06-25

Family

ID=40315830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/055306 WO2009077970A1 (fr) 2007-12-17 2008-12-15 Procédé d'obtention d'extraits aqueux contenant des caroténoïdes et extraits obtenus

Country Status (2)

Country Link
IT (1) ITRM20070651A1 (fr)
WO (1) WO2009077970A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016146837A1 (fr) * 2015-03-18 2016-09-22 Pierre Fabre Dermo-Cosmetique Procede de preparation d'un extrait de matrice vegetale avec un compose amphiphile non ionique comme adjuvant d'extraction en milieu aqueux
US9788561B2 (en) 2012-12-20 2017-10-17 Conopco, Inc. Tomato fibre composition and method for the preparation thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4316917A (en) * 1980-04-21 1982-02-23 Hoffman-La Roche Inc. Stable carotenoid solutions
WO1991018058A1 (fr) * 1990-05-16 1991-11-28 Kalamazoo Holdings, Inc. Compositions de pigment carotenoide a couleur stabilisee et denrees alimentaires ainsi colorees
WO2006036125A1 (fr) * 2004-09-28 2006-04-06 Gao Shen Sdn Bhd Procede pour la production d'extrait de lycopene

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4316917A (en) * 1980-04-21 1982-02-23 Hoffman-La Roche Inc. Stable carotenoid solutions
WO1991018058A1 (fr) * 1990-05-16 1991-11-28 Kalamazoo Holdings, Inc. Compositions de pigment carotenoide a couleur stabilisee et denrees alimentaires ainsi colorees
WO2006036125A1 (fr) * 2004-09-28 2006-04-06 Gao Shen Sdn Bhd Procede pour la production d'extrait de lycopene

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Biological enzyme extraction process of high-purity lycopene from tomato jam", DERWENT,, 1 January 1900 (1900-01-01), XP002337768 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9788561B2 (en) 2012-12-20 2017-10-17 Conopco, Inc. Tomato fibre composition and method for the preparation thereof
WO2016146837A1 (fr) * 2015-03-18 2016-09-22 Pierre Fabre Dermo-Cosmetique Procede de preparation d'un extrait de matrice vegetale avec un compose amphiphile non ionique comme adjuvant d'extraction en milieu aqueux
FR3033702A1 (fr) * 2015-03-18 2016-09-23 Pierre Fabre Dermo-Cosmetique Procede de preparation d'un extrait de plante avec un compose amphiphile non ionique comme adjuvant d'extraction en milieu aqueux
CN107404926A (zh) * 2015-03-18 2017-11-28 皮埃尔·法布尔皮肤化妆品公司 用于在水介质中用非离子两亲性化合物作为提取佐剂制备植物来源的基质的提取物的方法
US10299502B2 (en) 2015-03-18 2019-05-28 Pierre Fabre Dermo-Cosmetique Method for producing an extract of a matrix of vegetable origin with a non-ionic amphiphilic compound as extraction adjuvant in an aqueous medium
CN107404926B (zh) * 2015-03-18 2021-03-16 皮埃尔·法布尔皮肤化妆品公司 用于在水介质中用非离子两亲性化合物作为提取佐剂制备植物来源的基质的提取物的方法

Also Published As

Publication number Publication date
ITRM20070651A1 (it) 2009-06-18

Similar Documents

Publication Publication Date Title
Liu et al. Valorization of food waste to produce value-added products based on its bioactive compounds
Fierascu et al. Recovery of natural antioxidants from agro-industrial side streams through advanced extraction techniques
Dimou et al. Wine lees valorization: Biorefinery development including production of a generic fermentation feedstock employed for poly (3-hydroxybutyrate) synthesis
Costa et al. Apple pomace biorefinery: Integrated approaches for the production of bioenergy, biochemicals, and value-added products–An updated review
Matharu et al. Opportunity for high value-added chemicals from food supply chain wastes
Zhao et al. Potential use of peanut by-products in food processing: a review
Sangeetha et al. Extraction, characterization, and application of tomato seed oil in the food industry: An updated review
JP2012092032A (ja) セラミド産生促進剤
Caballero et al. Valorization of agro-industrial waste into bioactive compounds: techno-economic considerations
Rebouças et al. Shellfish industrial waste reuse
Nedović et al. Isolation, purification and encapsulation techniques for Bioactive Compounds from agricultural and Food production Waste
Wang et al. The high-value and sustainable utilization of grape pomace: A review
Azeez et al. Extraction and utilisation of bioactive compounds from agricultural waste
Krzyżostan et al. Use of waste from the food industry and applications of the fermentation process to create sustainable cosmetic products: A review
de Melo et al. Environmentally benign supercritical fluid extraction
Szopa et al. Waste valorization towards industrial products through chemo-and enzymatic-hydrolysis
Idrishi et al. Upcycling technologies in the food industry
WO2009077970A1 (fr) Procédé d'obtention d'extraits aqueux contenant des caroténoïdes et extraits obtenus
Thielemann et al. Introduction to the challenges and chances regarding the utilization of nitrogen-rich by-products and waste streams
Siddique et al. Exploration of novel eco-friendly techniques to utilize bioactive compounds from household food waste: special reference to food applications
Putra et al. Mini review of unlocking the hidden potential for valorization of dragon fruit peels through green extraction methods
Dhenge et al. Recent and novel technology used for the extraction and recovery of bioactive compounds from fruit and vegetable waste
JP6273551B2 (ja) アミロイド形成阻害作用を呈するネルボン酸誘導体
Martínez-Olivo et al. Advances in ultrasound-assisted extraction of bioactive compounds (antioxidant compounds) from agrofood waste
Fayaz et al. Food Processing Byproducts: Their Applications as Sources of Valuable Bioenergy and Recoverable Products

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08863274

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08863274

Country of ref document: EP

Kind code of ref document: A1

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载