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WO2008136253A1 - ウイルスベクターおよびその利用 - Google Patents

ウイルスベクターおよびその利用 Download PDF

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Publication number
WO2008136253A1
WO2008136253A1 PCT/JP2008/057170 JP2008057170W WO2008136253A1 WO 2008136253 A1 WO2008136253 A1 WO 2008136253A1 JP 2008057170 W JP2008057170 W JP 2008057170W WO 2008136253 A1 WO2008136253 A1 WO 2008136253A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
viral
protein
vector
sequence encoding
Prior art date
Application number
PCT/JP2008/057170
Other languages
English (en)
French (fr)
Inventor
Masashi Mori
Koji Dohi
Original Assignee
Japan Science And Technology Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science And Technology Agency filed Critical Japan Science And Technology Agency
Priority to AU2008246855A priority Critical patent/AU2008246855B2/en
Priority to EP08740267.3A priority patent/EP2154246B1/en
Priority to JP2009512912A priority patent/JP5070283B2/ja
Priority to US12/450,999 priority patent/US8586826B2/en
Priority to CA2689535A priority patent/CA2689535C/en
Publication of WO2008136253A1 publication Critical patent/WO2008136253A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

　ウイルスの塩基配列を含むポリヌクレオチドであって、該ウイルスの塩基配列は、ウイルスの複製タンパク質をコードする第１の塩基配列およびウイルスの移行タンパク質をコードする第２の塩基配列を含み、第２の塩基配列は第１の塩基配列の下流に位置しており、かつ発現させるべきポリペプチドをコードする外来塩基配列を連結するための連結部位を、第２の塩基配列の下流に有しており、第２の塩基配列は、ウイルス由来のネイティブな配列に対してさらなる塩基配列が挿入、置換または付加されたポリヌクレオチドを提供する。これにより、ウイルスの塩基配列を含むベクターを構築し、当該ベクターを含む宿主細胞の生育状態を低下させることなく、タンパク質を効率よく生産すること。
PCT/JP2008/057170 2007-04-27 2008-04-11 ウイルスベクターおよびその利用 WO2008136253A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2008246855A AU2008246855B2 (en) 2007-04-27 2008-04-11 Viral vector and use thereof
EP08740267.3A EP2154246B1 (en) 2007-04-27 2008-04-11 Viral vector and use thereof
JP2009512912A JP5070283B2 (ja) 2007-04-27 2008-04-11 ウイルスベクターおよびその利用
US12/450,999 US8586826B2 (en) 2007-04-27 2008-04-11 Virus vector and use thereof
CA2689535A CA2689535C (en) 2007-04-27 2008-04-11 Viral vector and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007120181 2007-04-27
JP2007-120181 2007-04-27

Publications (1)

Publication Number Publication Date
WO2008136253A1 true WO2008136253A1 (ja) 2008-11-13

Family

ID=39943370

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057170 WO2008136253A1 (ja) 2007-04-27 2008-04-11 ウイルスベクターおよびその利用

Country Status (6)

Country Link
US (1) US8586826B2 (ja)
EP (1) EP2154246B1 (ja)
JP (1) JP5070283B2 (ja)
AU (1) AU2008246855B2 (ja)
CA (1) CA2689535C (ja)
WO (1) WO2008136253A1 (ja)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3325502T3 (pl) * 2015-07-17 2021-01-11 Nunhems B.V. Nowy gatunek tobamowirusa

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005102652A (ja) 2003-10-01 2005-04-21 Japan Science & Technology Agency 形質転換細胞、および該細胞を用いたタンパク質の生産方法、並びにタンパク質生産キット
JP2005110594A (ja) 2003-10-08 2005-04-28 Japan Science & Technology Agency ウイルスベクター発現用ｄｎａ断片およびその利用
WO2005049839A2 (en) 2003-11-10 2005-06-02 Icon Genetics Ag Rna virus-derived plant expression system
JP2005245228A (ja) 2004-03-01 2005-09-15 Japan Science & Technology Agency タンパク質生産用形質転換体の生産方法、および当該生産方法によって得られたタンパク質生産用形質転換体、並びにその利用
WO2006003018A2 (en) 2004-07-07 2006-01-12 Icon Genetics Ag Biologically safe transient protein expression in plants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002006501A2 (en) * 2000-07-15 2002-01-24 The Samuel Roberts Noble Foundation, Inc. Method for altering degradation of engineered protein in plant cells
US20030036641A1 (en) * 2001-01-31 2003-02-20 Padgett Hal S. Methods for homology-driven reassembly of nucleic acid sequences
CA2540668C (en) * 2003-10-01 2010-12-21 Japan Science And Technology Agency Dna fragment, method for producting transformant for producing transformant for protein production and utilization thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005102652A (ja) 2003-10-01 2005-04-21 Japan Science & Technology Agency 形質転換細胞、および該細胞を用いたタンパク質の生産方法、並びにタンパク質生産キット
JP2005110594A (ja) 2003-10-08 2005-04-28 Japan Science & Technology Agency ウイルスベクター発現用ｄｎａ断片およびその利用
WO2005049839A2 (en) 2003-11-10 2005-06-02 Icon Genetics Ag Rna virus-derived plant expression system
JP2005245228A (ja) 2004-03-01 2005-09-15 Japan Science & Technology Agency タンパク質生産用形質転換体の生産方法、および当該生産方法によって得られたタンパク質生産用形質転換体、並びにその利用
WO2006003018A2 (en) 2004-07-07 2006-01-12 Icon Genetics Ag Biologically safe transient protein expression in plants

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
DOHI K. ET AL.: "Inducible Virus-mediated Expression of a Foreign Protein in Suspension-cultured Plant Cells", ARCH. VIROL., vol. 151, no. 6, 2006, pages 1075 - 1084, XP019430522 *
DOHI, K. ET AL., ARCH. VIROL., vol. 151, 2006, pages 1075 - 1084
E. JOHANSEN, PNAS. USA, vol. 93, 1996, pages 12400 - 12405
GILLESPIE T. ET AL.: "Functional Analysis of a DNA-shuffled Movement Protein Reveals that Microtubules are Dispensable for the Cell-to-cell Movement of Tobacco Mosaic Virus", THE PLANT CELL, vol. 14, no. 6, 2002, pages 1207 - 1222, XP008123708 *
GILLESPIE. T. ET AL., THE PLANT CELL, vol. 14, 2002, pages 1207 - 1222
JOHANSSEN E., PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 12400 - 12405
MAN M. ET AL.: "Assessment of the Effectiveness of a Nuclear-launched TMV-based Replicon as a Tool for Foreign Gene Expression in Plants in Comparison to Direct Gene Expression from a Nuclear Promoter", TRANSGENIC RES., vol. 15, no. 1, 2006, pages 107 - 113, XP019269505 *
S. J. YANG ET AL., ARCH VIROL, vol. 143, 1998, pages 2443 - 2451
S. MARILLONNET ET AL., PNAS, vol. 101, no. 18, 2004, pages 6852 - 6857
SATYANARAYANA, T. ET AL., VIROLOGY, vol. 313, 2003, pages 481 - 491
See also references of EP2154246A4
TOTH R.L. ET AL.: "Improvement of the Movement and Host Range Properties of a Plant Virus Vector through DNA Shuffling", PLANT J., vol. 30, no. 5, 2002, pages 593 - 600, XP002286330 *
TOTH, R. L. ET AL., THE PLANT JOURNAL, vol. 30, 2002, pages 593 - 600
YAMAYA Y. ET AL., MOL. GEN. GENET., vol. 211, 1988, pages 520 - 525

Also Published As

Publication number Publication date
CA2689535A1 (en) 2008-11-13
US8586826B2 (en) 2013-11-19
EP2154246B1 (en) 2017-03-29
AU2008246855A1 (en) 2008-11-13
JPWO2008136253A1 (ja) 2010-07-29
EP2154246A4 (en) 2010-10-27
CA2689535C (en) 2015-05-19
US20110107466A1 (en) 2011-05-05
AU2008246855B2 (en) 2011-05-26
JP5070283B2 (ja) 2012-11-07
EP2154246A1 (en) 2010-02-17

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