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WO2008127012A1 - Méthode de mise au point pour biopuce - Google Patents

Méthode de mise au point pour biopuce Download PDF

Info

Publication number
WO2008127012A1
WO2008127012A1 PCT/KR2008/002005 KR2008002005W WO2008127012A1 WO 2008127012 A1 WO2008127012 A1 WO 2008127012A1 KR 2008002005 W KR2008002005 W KR 2008002005W WO 2008127012 A1 WO2008127012 A1 WO 2008127012A1
Authority
WO
WIPO (PCT)
Prior art keywords
disk
area
biochip
focus
interval
Prior art date
Application number
PCT/KR2008/002005
Other languages
English (en)
Inventor
Soo-Kyung Kim
Won-Hyung Cho
Original Assignee
Nanostorage Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanostorage Co., Ltd. filed Critical Nanostorage Co., Ltd.
Publication of WO2008127012A1 publication Critical patent/WO2008127012A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B7/00—Recording or reproducing by optical means, e.g. recording using a thermal beam of optical radiation by modifying optical properties or the physical structure, reproducing using an optical beam at lower power by sensing optical properties; Record carriers therefor
    • G11B7/08—Disposition or mounting of heads or light sources relatively to record carriers
    • G11B7/09—Disposition or mounting of heads or light sources relatively to record carriers with provision for moving the light beam or focus plane for the purpose of maintaining alignment of the light beam relative to the record carrier during transducing operation, e.g. to compensate for surface irregularities of the latter or for track following
    • G11B7/0908—Disposition or mounting of heads or light sources relatively to record carriers with provision for moving the light beam or focus plane for the purpose of maintaining alignment of the light beam relative to the record carrier during transducing operation, e.g. to compensate for surface irregularities of the latter or for track following for focusing only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence

Definitions

  • the present invention relates to a focus adjusting method for a biochip, and more particularly, to a focus adjusting method for a biochip, that prevents a data acquisition error by acquiring data after previously calculating a data area and a non-data area and thus rapidly adjusting a focus in the data area.
  • a biochip can be classified into a DNA chip using DNA probes, a protein chip using proteins such as enzymes, antigens/antibodies and bacteri- orhodopsins, a cell chip using cells, and a lab-on-a-chip.
  • the lab-on-a-chip is used when processing a large quantity of samples and has a meaning of "a laboratory on a chip" since all pretreatments including separation, refining and amplification of samples and their analysis are implemented on a single chip.
  • One example of an analyzing method using the biochip is to check whether diseases or symptoms exist by analyzing revelation of a fluorescent material caused by the reaction of protein contained in blood or urine of an inspector, after reacting the blood or urine on a biochip.
  • the biochip is integrated with protein and a fluorescent material that act on specific diseases or symptoms.
  • the biochip for diagnosing specific diseases or symptoms is called a diagnostic kit.
  • the diagnostic kit has been widely used because of its rapidity and accuracy.
  • the above-described analysis method using the biochip has been implemented through eyes of an analyzer or a microscope.
  • the monitored time (that is, an analysis time of the biochip) depends on the size of a cell constituting the biochip.
  • the size of the cell is determined according to a used purpose by the accuracy and capacity of desired information and ranges from a few m to several hundred m. Therefore, the analysis time of commercially available biochip products takes a few minutes to several tens of minutes.
  • the fluorescence confocal scanner is suitable for a chip that requires accuracy of 1 m or less or monitors a large quantity of information, it is difficult to widely use the biochip.
  • the present invention has been made in view of the above problems, and it is an object of the present invention to provide a focus adjusting method for a biochip, that prevents a data acquisition error by acquiring data after previously calculating a data area and a non-data area and thus rapidly adjusting a focus in the data area.
  • a data acquisition error is prevented by acquiring data after previously calculating a data area and a non-data area and thus rapidly adjusting a focus in the data area.
  • a data acquisition time is shortened by obtaining data after a location of a data area is previously confirmed.
  • a focus adjusting time is shortened and vibration generated when a focus is adjusted is minimized, since the focus is previously adjusted through a sensing means before a scanner approaches a biochip area within the biochip area and a disk area out of the entire area of a disk.
  • FIG. 1 is a block diagram illustrating a focus adjusting method for a biochip according to the present invention
  • Fig. 2 is a plan view illustrating a disk in which biochips are installed according to the present invention
  • Fig. 3 is a partial cross-sectional view illustrating a disk in which biochips are installed according to the present invention
  • Fig. 4 is a graph illustrating a disk area scanned at a low speed according to the present invention
  • Fig. 5 is an enlarged graph of a biochip interval and a disk interval in a scanning area shown in Fig. 3;
  • FIG. 6 is a graph illustrating a biochip interval and a disk interval including focus information according to the present invention.
  • Fig. 7 is a plan view illustrating a biochip holder area and a biochip area according to the present invention.
  • Fig. 8 is a partial enlarged cross-sectional view illustrating a disk shown in Fig. 7; and
  • Fig. 9 is a plan view illustrating a disk according to another exemplary embodiment of the present invention.
  • a focus adjusting method includes: 1) scanning a disk in which at least one biochip is installed and storing scanning information on an entire area of the disk: 2) judging a data area and a disk area based on the scanning information; 3) calculating a location value of the data area; 4) adjusting a focus of a scanner prior to the data area, based on the location value of the data area; and 5) recording or reproducing information on the biochip by using the scanner of which focus is adjusted.
  • the steps 1) and 2) acquire focus information on a biochip area and the disk area by scanning the disk in which the biochip is installed.
  • the step 3) discriminates between a location for the data area and a location for the disk area out of the entire area of the disk, based on the scanning information obtained from the steps 1) and 2).
  • the disk area further includes focus information on a gap interval between the disk area and a biochip area.
  • the step 3) applies a value obtained by dividing a sum of a start interval and an end interval of the data area out of a biochip scanning interval to the disk area or a gap interval.
  • the steps 1) and 2) separately scan a scanning interval and a non-scanning in terval in the entire area of the disk.
  • the steps 1) to 3) cause the scanner to acquire focus information on the entire area of the disk while the disk rotates at a low speed.
  • the steps 4) and 5) record or reproduce information on the biochip by more increasing a rotation speed of the disk than a rotation speed at the steps 1) to 3), based on data on the entire area of the disk acquired in the steps 1) to 3).
  • the step 2) separately judges a biochip holder area based on the scanning information, and the biochip holder area is replaced by the judged biochip area.
  • a sensing means for confirming the disk area and the data area on the disk is provided, and the sensing means causes the scanner to adjust the focus prior to a start point of the data area.
  • the sensing means comprises a first sensor and a first signal generator for confirming the number of rotations of the disk, and a second sensor and a second signal generator for confirming respective data area during rotation of the disk.
  • the second signal generator is provided between the respective data areas.
  • a focus adjusting method for a biochip includes scanning and storing the entire area of a disk (SlO), judging a data area and a disk area (S20), calculating a location value of the data area (S30), adjusting a focus of a scanner (S40), and scanning the biochip (S50).
  • focus information is acquired by scanning the entire area of a disk 10 shown in Fig. 2 by a scanner. That is, the entire area of the disk is scanned by operating the scanner under the state that the disk 10 rotates at a low speed, and focus information for the entire area of the disk is acquired and stored.
  • a laser beam emitted from the scanner is irradiated onto the entire area of the disk and focus values for the entire area of the disk are acquired and stored.
  • the stored focus values include focus values for a biochip interval 110, a disk interval 120, and a non-scanning interval 200.
  • the biochip interval 110 and the disk area interval 120 are judged based on the focus information acquired by scanning the entire area of the disk. As shown in Fig. 3 or Fig. 4, the irradiated laser beam is reflected in the biochip interval out of the scanned entire area of the disk but it passes through the disk interval. Therefore, focus values in the disk interval and the biochip interval are different and this is judged by a scanning location and time according to the rotation speed of the disk.
  • the disk interval 120 has focus information similar to the biochip interval
  • the entire area of the disk is divided into the biochip interval 110 and the disk interval 120 and the divided biochip interval and disk interval are separately stored.
  • a focus value in the disk interval 120 is adjusted based on information of the disk interval and biochip interval judged in the second step S20.
  • the biochip interval 110 acquired in the second step S20 includes a data area 112 for recording/reproducing data.
  • a start interval FA2 and an end interval FAl of the data area out of the biochip interval 120 can be calculated.
  • the scanner focuses a track location for recording data or a reproducing location in the data area to determine a location for the data area.
  • a value obtained by adding and dividing values of the start interval FA2 and the end interval FAl except for the data area 112 within the biochip interval is applied to an area where the scanner is not focused.
  • the above-described process may be applicable to the disk interval 120.
  • the gap interval 122 can be focused by applying a value obtained by adding and dividing the values of the start interval FA2 and the end interval FAl of the data area.
  • the entire area of the disk is scanned based on focus information on the disk interval 120 and the biochip interval 110 acquired in the third step S30 and focus information obtained in the biochip interval. That is, focus in- formation on respective intervals of the disk is inputted through the focus information on the entire area of the disk that is previously obtained in step S30 and then the scanner moves toward the biochip interval. Accordingly, a focus time in the biochip interval is shortened, thereby preventing an error in the data interval of the biochip interval while recording/reproducing bioinformation.
  • step S50 a recording/reproducing operation is performed at the same time when the scanner enters the data area 112 after a focus of the scanner is adjusted in step S40.
  • the biochips are installed in only a part of the biochip holders 130. If the biochips are installed in the biochip holders 130, the above scanning method is applied and a focus is adjusted. However, if the biochips are not installed in the biochip holders 130 and thus the biochip holders 130 remain as empty spaces, a biochip holder area is separately judged based on the scanning information acquired in step SlO, and the biochip holder area 132 is replaced by the judged biochip area and then focused.
  • the biochip interval 110 and the holder area 132 are judged under the state that the entire area of the disk is scanned.
  • the biochip holder area 132 has the same focus value as the disk area 120 as illustrated in Fig. 4.
  • a value calculated when scanning the biochip interval 110 is applied to the biochip holder area 132 in which the biochips are not installed. That is, a focus is previously adjusted, vibration generated during focus adjustment is minimized and a focus adjusting time is shortened.
  • the disk 10 further includes a sensing means 300 for confirming a location of the biochip area 110.
  • the sensing means 300 causes the scanner to adjust a focus in the disk area 120 before the data area 110 is started.
  • the sensing means 300 includes a first sensor 310 and a first signal generator 311 that confirm the number of rotations of the disk and a second sensor 320 and second signal generators 320a, 320b, , 32On that confirm each data area 110 during disk rotation.
  • the second signal generators are provided between respective data areas.
  • the rotating state of the disk is confirmed by the first sensor 310 and the first signal generator 311, and start and end points of the data area are confirmed by the second sensor 320 and the second signal generators 320a, 320b, and 32On. That is, if the first and second sensors 310 and 320 sense signals under the state that the first sensor 310 and the first signal generator 311 approximates to each other and the second sensor 320 and the second signal generator 320a approximates to each other, that location is determined as a start location of the first data area.
  • the second signal generator 320b is located toward the second sensor 320. At this time, an area between the second signal generators 320a and 320b is determined as the data area and the second signal generator 320b is determined as a start location of the second data area interval.
  • the above sensing operations are repeated, and if the first signal generator 311 senses the first sensor 310 twice, an area between the second signal generators 32On and 320a is confirmed as the last data area and thus the data area interval and the disk area can be confirmed. Therefore, the focus of the scanner can be previously adjusted when the scanner moves to the data area from the disk area.
  • the data area and the disk area can be confirmed by using the first and second sensors and the first and second signal generators and data in the data area can be stored.
  • the first and second signal generators may use sensor dogs or plates, and the first and second sensors may use photosensors.
  • the first and second signal generators are provided on the disk.
  • a scanner (not shown) and a focusing module (not shown) are located at the upper side of the disk 10.
  • the scanner and the focusing module can move in horizontal and vertical directions as well known in the art.
  • the disk 10 rotates at a low speed while a laser beam is irradiated through the scanner. Then the scanner irradiates the laser beam on a first scanning interval 100a and a laser beam signal entered by reflecting the irradiated laser beam through the rotating disk is applied to the focusing module, thereby adjusting a focus and acquiring focus information on a scanning interval.
  • biochips are installed in the disk at regular intervals based on an outer circumference and a plurality of chips for recording/reproducing bioinformation is installed in the biochip 20.
  • Non-scanning intervals 200a, 200b, 200c and 20On alternate with the scanning intervals and the respective intervals correspond to the number of chips provided in the biochip.
  • the biochip interval is divided into a chip area in which data is actually recorded/reproduced and a non-chip area.
  • focus values of a start area and an end area of the chip are added to each other and the added focus value is applied to the disk interval.
  • the scanner approaches the data area with the previously adjusted focus. Consequently, a focusing time is shortened and a focusing error is prevented.
  • focus values for the biochip interval and the disk interval are applied to the scanner and the focusing module. Scanning is performed based on the applied information while the disk rotates. A data recording/reproducing time in the biochip is shortened by more increasing a rotation speed of the disk than a rotation speed during the first scanning. Since the focus value for the entire area of the disk is applied to the scanner and the focusing module, the scanner can be shifted in a focus adjusted state even in a non-data area and a focus can be rapidly adjusted in the data area, thereby recording/reproducing data while the disk rotates at a high speed.
  • focus information acquired in the scanning interval based on the first scanning interval has been described, focus information for the second, third, and nth scanning intervals may be achieved in the same way.
  • data may be included in the data area by confirming the data area and the disk area using the first and second sensors and the first and second signal generators.

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Optical Recording Or Reproduction (AREA)

Abstract

L'invention porte sur une méthode de mise au point pour biopuce consistant: 1) à balayer un disque dans lequel au moins une biopuce est installée et à stocker les informations de balayage portant sur la totalité d'une zone du disque; 2) à évaluer une zone de données et une zone de disque sur la base des informations de balayage; 3) à calculer une valeur d'emplacement de la zone de données; 4) à faire la mise au point d'un scanneur avant la zone de données, sur la base de la valeur d'emplacement de la zone de données; et 5) à enregistrer ou reproduire les informations sur la biopuce en utilisant le scanneur après sa mise au point. L'invention permet d'empêcher les erreurs d'acquisition de données en acquérant les données après avoir calculé une zone avec données et une zone sans données, puis en effectant une msie au point rapide dans la zone de données.
PCT/KR2008/002005 2007-04-12 2008-04-10 Méthode de mise au point pour biopuce WO2008127012A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0035894 2007-04-12
KR20070035894 2007-04-12

Publications (1)

Publication Number Publication Date
WO2008127012A1 true WO2008127012A1 (fr) 2008-10-23

Family

ID=39864072

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/002005 WO2008127012A1 (fr) 2007-04-12 2008-04-10 Méthode de mise au point pour biopuce

Country Status (2)

Country Link
KR (1) KR100992553B1 (fr)
WO (1) WO2008127012A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130099703A (ko) 2012-02-29 2013-09-06 삼성전자주식회사 옵티컬 헤드 및 이를 포함하는 시퀀싱 장치

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100339379B1 (ko) * 1999-10-29 2002-06-03 구자홍 바이오칩과 그의 생체 물질 측정장치 및 측정방법
US6407395B1 (en) * 2000-02-29 2002-06-18 The University Of Chicago Portable biochip scanner device
KR100379411B1 (ko) * 1999-06-28 2003-04-10 엘지전자 주식회사 바이오칩 및 그의 생체 물질 패터닝 및 측정 방법
US20050179894A1 (en) * 2004-02-13 2005-08-18 Jiann-Hua Wang Fluorescent microarray analyzer
US7095032B2 (en) * 1998-03-20 2006-08-22 Montagu Jean I Focusing of microscopes and reading of microarrays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7095032B2 (en) * 1998-03-20 2006-08-22 Montagu Jean I Focusing of microscopes and reading of microarrays
KR100379411B1 (ko) * 1999-06-28 2003-04-10 엘지전자 주식회사 바이오칩 및 그의 생체 물질 패터닝 및 측정 방법
KR100339379B1 (ko) * 1999-10-29 2002-06-03 구자홍 바이오칩과 그의 생체 물질 측정장치 및 측정방법
US6407395B1 (en) * 2000-02-29 2002-06-18 The University Of Chicago Portable biochip scanner device
US20050179894A1 (en) * 2004-02-13 2005-08-18 Jiann-Hua Wang Fluorescent microarray analyzer

Also Published As

Publication number Publication date
KR20080092854A (ko) 2008-10-16
KR100992553B1 (ko) 2010-11-08

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