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WO2008115844A3 - Stable, functional chimeric cytochrome p450 holoenzymes - Google Patents

Stable, functional chimeric cytochrome p450 holoenzymes Download PDF

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Publication number
WO2008115844A3
WO2008115844A3 PCT/US2008/057174 US2008057174W WO2008115844A3 WO 2008115844 A3 WO2008115844 A3 WO 2008115844A3 US 2008057174 W US2008057174 W US 2008057174W WO 2008115844 A3 WO2008115844 A3 WO 2008115844A3
Authority
WO
WIPO (PCT)
Prior art keywords
holoenzymes
stable
functional chimeric
cytochrome
chimeric cytochrome
Prior art date
Application number
PCT/US2008/057174
Other languages
French (fr)
Other versions
WO2008115844A2 (en
Inventor
Frances H Arnold
Yougen Li
Original Assignee
California Inst Of Techn
Frances H Arnold
Yougen Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/024,515 external-priority patent/US20080248545A1/en
Application filed by California Inst Of Techn, Frances H Arnold, Yougen Li filed Critical California Inst Of Techn
Publication of WO2008115844A2 publication Critical patent/WO2008115844A2/en
Publication of WO2008115844A3 publication Critical patent/WO2008115844A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present disclosure relates to cytochrome p450 fusion polypetides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
PCT/US2008/057174 2007-03-16 2008-03-15 Stable, functional chimeric cytochrome p450 holoenzymes WO2008115844A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US91852807P 2007-03-16 2007-03-16
US60/918,528 2007-03-16
US12/024,515 2008-02-01
US12/024,515 US20080248545A1 (en) 2007-02-02 2008-02-01 Methods for Generating Novel Stabilized Proteins
US2788508A 2008-02-07 2008-02-07
US12/027,885 2008-02-07

Publications (2)

Publication Number Publication Date
WO2008115844A2 WO2008115844A2 (en) 2008-09-25
WO2008115844A3 true WO2008115844A3 (en) 2009-05-14

Family

ID=39766722

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/057174 WO2008115844A2 (en) 2007-03-16 2008-03-15 Stable, functional chimeric cytochrome p450 holoenzymes

Country Status (1)

Country Link
WO (1) WO2008115844A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
EP2069513A4 (en) 2006-08-04 2012-03-21 California Inst Of Techn METHODS AND SYSTEMS FOR SELECTIVE FLUORATION OF ORGANIC MOLECULES
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8802401B2 (en) 2007-06-18 2014-08-12 The California Institute Of Technology Methods and compositions for preparation of selectively protected carbohydrates
EP2426198A1 (en) 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Cytochrome P450 monooxygenase variants
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
CN116790529B (en) * 2023-05-24 2025-01-17 天津大学 P450 BM3 protein mutant and its application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017106A2 (en) * 2003-06-17 2005-02-24 California Institute Of Technology Libraries of optimized cytochrome p450 enzymes and the optimized p450 enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017106A2 (en) * 2003-06-17 2005-02-24 California Institute Of Technology Libraries of optimized cytochrome p450 enzymes and the optimized p450 enzymes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LANDWEHR ET AL: "Diversification of Catalytic Function in a Synthetic Family of Chimeric Cytochrome P450s", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 14, no. 3, 23 March 2007 (2007-03-23), pages 269 - 278, XP005929291, ISSN: 1074-5521 *
MEYER MICHELLE M ET AL: "Library analysis of SCHEMA-guided protein recombination.", PROTEIN SCIENCE, vol. 12, no. 8, August 2003 (2003-08-01), pages 1686 - 1693, XP002502197, ISSN: 0961-8368 *
OTEY CHRISTOPHER R ET AL: "Structure-guided recombination creates an artificial family of cytochromes P450", PLOS BIOLOGY, vol. 4, no. 5, May 2006 (2006-05-01), pages 789 - 798, XP002502196, ISSN: 1544-9173(print) 1545-7885(ele *

Also Published As

Publication number Publication date
WO2008115844A2 (en) 2008-09-25

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