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WO2008115517A3 - Méthodes d'identification de cellules souches par détection de l'autofluorescence des cellules et du syncytium - Google Patents

Méthodes d'identification de cellules souches par détection de l'autofluorescence des cellules et du syncytium Download PDF

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Publication number
WO2008115517A3
WO2008115517A3 PCT/US2008/003604 US2008003604W WO2008115517A3 WO 2008115517 A3 WO2008115517 A3 WO 2008115517A3 US 2008003604 W US2008003604 W US 2008003604W WO 2008115517 A3 WO2008115517 A3 WO 2008115517A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
syncytia
methods
stem cells
identifying stem
Prior art date
Application number
PCT/US2008/003604
Other languages
English (en)
Other versions
WO2008115517A2 (fr
Inventor
Elena V Gostjeva
William G Thilly
Original Assignee
Massachusetts Inst Technology
Elena V Gostjeva
William G Thilly
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology, Elena V Gostjeva, William G Thilly filed Critical Massachusetts Inst Technology
Publication of WO2008115517A2 publication Critical patent/WO2008115517A2/fr
Publication of WO2008115517A3 publication Critical patent/WO2008115517A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention porte sur des procédés de caractérisation du stade de développement ou de la pathologie d'un échantillon de tissu et d'identification de cellules souches pluripotentes, qui consistent à détecter l'autofluorescence des cellules et du syncytium.
PCT/US2008/003604 2007-03-19 2008-03-19 Méthodes d'identification de cellules souches par détection de l'autofluorescence des cellules et du syncytium WO2008115517A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91880807P 2007-03-19 2007-03-19
US60/918,808 2007-03-19

Publications (2)

Publication Number Publication Date
WO2008115517A2 WO2008115517A2 (fr) 2008-09-25
WO2008115517A3 true WO2008115517A3 (fr) 2009-03-12

Family

ID=39529613

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/003604 WO2008115517A2 (fr) 2007-03-19 2008-03-19 Méthodes d'identification de cellules souches par détection de l'autofluorescence des cellules et du syncytium

Country Status (1)

Country Link
WO (1) WO2008115517A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009860A2 (fr) 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methode permettant d'identifier les cellules souches a partir des morphotypes nucleaires
US8951938B2 (en) 2007-12-20 2015-02-10 New York University Combinatorial rosamine library to detect cell-state switching
KR20110079674A (ko) 2008-09-24 2011-07-07 메사추세츠 인스티튜트 오브 테크놀로지 세포 및 융합 세포의 형광을 검출함으로써 줄기 세포를 확인하는 방법
WO2012027266A2 (fr) * 2010-08-24 2012-03-01 New York University Procédés de détection de cellules souches embryonnaires, de cellules souches pluripotentes induites ou de cellules subissant une reprogrammation pour produire des cellules souches pluripotentes induites
CA2815787A1 (fr) 2010-10-25 2012-05-10 Massachusetts Institute Of Technology Cellules souches metacaryotes pour la cicatrisation des plaies et leurs procedes d'utilisation
ES2655433T3 (es) * 2012-11-12 2018-02-20 National Centre For Biological Sciences Un procedimiento para identificar y aislar células madre pluripotentes utilizando fluorescencia azul endógena
CN109254142B (zh) * 2018-11-08 2025-04-15 贵阳德漫多医疗科技有限公司 一种识别肿瘤干细胞并检测其药物敏感性的系统及其检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009860A2 (fr) * 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methode permettant d'identifier les cellules souches a partir des morphotypes nucleaires

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006009860A2 (fr) * 2004-06-17 2006-01-26 Massachusetts Institute Of Technology Methode permettant d'identifier les cellules souches a partir des morphotypes nucleaires

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GOSTJEVA E V ET AL: "Nuclear morphotypes in human embryogenesis and carcinogenesis: Bell-shaped nuclei show stem-like properties in vivo.", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, vol. 47, no. 6, July 2006 (2006-07-01), & 37TH ANNUAL MEETING OF THE ENVIRONMENTAL-MUTAGEN-SOCIETY; VANCOUVER, CANADA; SEPTEMBER 16 -20, 2006, pages 405, XP009102297, ISSN: 0893-6692 *
GOSTJEVA ET AL: "Bell-shaped nuclei dividing by symmetrical and asymmetrical nuclear fission have qualities of stem cells in human colonic embryogenesis and carcinogenesis", CANCER GENETICS AND CYTOGENETICS, ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY, US, vol. 164, no. 1, 1 January 2006 (2006-01-01), pages 16 - 24, XP005214800, ISSN: 0165-4608 *

Also Published As

Publication number Publication date
WO2008115517A2 (fr) 2008-09-25

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