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WO2008152640A3 - Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches - Google Patents

Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches Download PDF

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Publication number
WO2008152640A3
WO2008152640A3 PCT/IL2008/000803 IL2008000803W WO2008152640A3 WO 2008152640 A3 WO2008152640 A3 WO 2008152640A3 IL 2008000803 W IL2008000803 W IL 2008000803W WO 2008152640 A3 WO2008152640 A3 WO 2008152640A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
hematopoietic stem
dimensional
cell culture
stromal cell
Prior art date
Application number
PCT/IL2008/000803
Other languages
English (en)
Other versions
WO2008152640A2 (fr
Inventor
Shai Meretzki
Uriel Barkai
Original Assignee
Pluristem Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pluristem Ltd. filed Critical Pluristem Ltd.
Publication of WO2008152640A2 publication Critical patent/WO2008152640A2/fr
Publication of WO2008152640A3 publication Critical patent/WO2008152640A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention porte sur un procédé de transplantation de cellules souches hématopoïétiques non différenciées sur un receveur. Le procédé consiste à (a) faire se développer les cellules souches hématopoïétiques non différenciées pour augmenter le nombre des cellules souches hématopoïétiques par (i) la culture dans un bioréacteur à écoulement piston en phase stationnaire de cellules stromales sous un courant continu d'un milieu de culture établi sur un substrat biocompatible tridimensionnel afin de générer une culture tridimensionnelle de cellules stromales ; et (ii) l'ensemencement de cellules souches hématopoïétiques non différenciées dans le bioréacteur à écoulement piston en phase stationnaire comprenant la culture tridimensionnelle de cellules stromales et sous un courant continu d'un milieu de culture pour développer les cellules souches hématopoïétiques non différenciées et obtenir une culture tridimensionnelle de cellules stromales comprenant un nombre accru de cellules souches hématopoïétique ; et (b) transplanter sur le receveur la culture tridimensionnelle de cellules stromales comprenant des cellules souches hématopoïétiques résultant de (a).
PCT/IL2008/000803 2007-06-13 2008-06-12 Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches WO2008152640A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92909707P 2007-06-13 2007-06-13
US60/929,097 2007-06-13

Publications (2)

Publication Number Publication Date
WO2008152640A2 WO2008152640A2 (fr) 2008-12-18
WO2008152640A3 true WO2008152640A3 (fr) 2010-02-25

Family

ID=40130285

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000803 WO2008152640A2 (fr) 2007-06-13 2008-06-12 Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches

Country Status (1)

Country Link
WO (1) WO2008152640A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9198938B2 (en) 2008-11-19 2015-12-01 Antrhogenesis Corporation Amnion derived adherent cells

Families Citing this family (12)

* Cited by examiner, † Cited by third party
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KR20240052847A (ko) 2008-08-20 2024-04-23 셀룰래리티 인코포레이티드 개선된 세포 조성물 및 그의 제조 방법
GB0822246D0 (en) * 2008-12-05 2009-01-14 Reneuron Ltd Composition
GB0902034D0 (en) 2009-02-06 2009-03-11 Reneuron Ltd Method
AU2011352036A1 (en) 2010-12-31 2013-07-18 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
DK2714059T3 (en) 2011-06-01 2019-01-21 Celularity Inc TREATMENT OF PAIN WHEN USING PLACENT STAM CELLS
EP2741757B1 (fr) 2011-09-11 2018-05-16 Minovia Therapeutics Ltd. Compositions de mitochondries fonctionnelles et leurs utilisations
US10702556B2 (en) 2012-05-16 2020-07-07 Minovia Therpautices Ltd. Compositions and methods for inducing angiogenesis
JP7015169B2 (ja) 2015-02-26 2022-02-02 ミノヴィア セラピューティクス リミテッド 機能性ミトコンドリアで富化された哺乳動物細胞
WO2020021539A1 (fr) 2018-07-22 2020-01-30 Minovia Therapeutics Ltd. Thérapie d'augmentation mitochondriale de maladies musculaires
EP3823641A4 (fr) 2018-07-22 2022-05-18 Minovia Therapeutics Ltd. Thérapie d'augmentation mitochondriale de maladies oculaires
US20240240133A1 (en) 2021-05-27 2024-07-18 Bühler AG Optimized industrial bioreactor and method thereof, with mutually dependent, coupled process control loops
EP4095626A1 (fr) 2021-05-27 2022-11-30 Bühler AG Bioréacteur industriel optimisé et procédé associé, avec des boucles de commande de processus couplées mutuellement dépendantes

Citations (4)

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Publication number Priority date Publication date Assignee Title
US6498138B1 (en) * 1998-03-11 2002-12-24 University Of Southern California Method of promoting production of living tissue equivalents
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050180958A1 (en) * 1999-02-04 2005-08-18 Technion Research & Development Foundation Ltd. Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6498138B1 (en) * 1998-03-11 2002-12-24 University Of Southern California Method of promoting production of living tissue equivalents
US20050180958A1 (en) * 1999-02-04 2005-08-18 Technion Research & Development Foundation Ltd. Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OGATA ET AL.: "Identification and Hematopoietic Potential of CD45? Clonal Cells with Very Immature Phenotype (CD45?CD34?CD38?Lin?) in Patients with Myelodysplastic Syndromes", STEM CELLS., vol. 23, 2005, pages 619 - 630 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9198938B2 (en) 2008-11-19 2015-12-01 Antrhogenesis Corporation Amnion derived adherent cells

Also Published As

Publication number Publication date
WO2008152640A2 (fr) 2008-12-18

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