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WO2008146854A1 - 抗モータリン抗体のパラトープ及びエピトープ - Google Patents

抗モータリン抗体のパラトープ及びエピトープ Download PDF

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Publication number
WO2008146854A1
WO2008146854A1 PCT/JP2008/059834 JP2008059834W WO2008146854A1 WO 2008146854 A1 WO2008146854 A1 WO 2008146854A1 JP 2008059834 W JP2008059834 W JP 2008059834W WO 2008146854 A1 WO2008146854 A1 WO 2008146854A1
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
mortalin
epitope
chain
paratope
Prior art date
Application number
PCT/JP2008/059834
Other languages
English (en)
French (fr)
Inventor
Renu Wadhwa
Sunil Kaul
Maki Shiota
Atsushi Inoue
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2008036343A external-priority patent/JP5142265B2/ja
Application filed by National Institute Of Advanced Industrial Science And Technology filed Critical National Institute Of Advanced Industrial Science And Technology
Priority to US12/602,358 priority Critical patent/US8591893B2/en
Publication of WO2008146854A1 publication Critical patent/WO2008146854A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6843—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0058—Antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
    • A61K49/0065—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle
    • A61K49/0067—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle quantum dots, fluorescent nanocrystals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Inorganic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Nanotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

　細胞内在性抗モータリン抗体と非内在性抗モータリン抗体とのＬ鎖及びＨ鎖それぞれの可変領域に存在する、抗モータリン抗体の腫瘍細胞への内在化機能に関与するパラトープ領域のアミノ酸配列を決定した。当該パラトープ領域を有するＬ鎖及びＨ鎖の可変領域をペプチドリンカーで繋いだ一本鎖抗体（scFv）のモータリンに対する結合活性を利用して、癌細胞特異的な薬剤デリバリーを提供した。また、６アミノ酸からなる内在化機能を有する抗モータリン抗体が認識するエピトープのアミノ酸配列を決定し、当該エピトープをコードする核酸を含む発現ベクターを用いることで、モータリン抗体及びそれに結合させた薬剤などの、癌細胞への内在化促進剤を提供できた。
PCT/JP2008/059834 2007-05-28 2008-05-28 抗モータリン抗体のパラトープ及びエピトープ WO2008146854A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/602,358 US8591893B2 (en) 2007-05-28 2008-05-28 Paratope and epitope of anti-mortalin antibody

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2007140943 2007-05-28
JP2007-140943 2007-05-28
JP2007296405 2007-11-15
JP2007-296405 2007-11-15
JP2008036343A JP5142265B2 (ja) 2007-05-28 2008-02-18 抗モータリン抗体のパラトープ及びエピトープ
JP2008-036343 2008-02-18

Publications (1)

Publication Number Publication Date
WO2008146854A1 true WO2008146854A1 (ja) 2008-12-04

Family

ID=40075091

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059834 WO2008146854A1 (ja) 2007-05-28 2008-05-28 抗モータリン抗体のパラトープ及びエピトープ

Country Status (1)

Country Link
WO (1) WO2008146854A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011071099A1 (ja) * 2009-12-10 2011-06-16 独立行政法人産業技術総合研究所 抗癌性抗モータリンペプチド抗体
WO2012057328A1 (ja) * 2010-10-29 2012-05-03 株式会社ペルセウスプロテオミクス 高い内在化能力を有する抗ｃｄｈ３抗体
EP2700652A4 (en) * 2011-04-18 2015-07-15 Univ Tokyo DIAGNOSIS AND TREATMENT OF CANCER USING ANTI-ITM2A ANTIBODY
WO2017037707A1 (en) * 2015-09-02 2017-03-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Antibodies specific to human t-cell immunoglobulin and itim domain (tigit)
EP3221350A4 (en) * 2014-11-20 2018-08-08 Ramot at Tel-Aviv University Ltd. Mortalin peptides and antibodies and uses thereof for inhibiting mortalin activity and treating a disease associated with a pathological cell

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08500084A (ja) * 1992-05-11 1996-01-09 イムノメディクス，インコーポレイテッド 毒素および薬剤を含む治療用複合体
JP2005500808A (ja) * 2000-12-06 2005-01-13 ニユーララブ・リミテツド βアミロイドペプチドを認識するヒト化抗体
JP2006089471A (ja) * 2004-08-26 2006-04-06 National Institute Of Advanced Industrial & Technology 癌の治療における抗モータリン２抗体と機能性核酸の使用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08500084A (ja) * 1992-05-11 1996-01-09 イムノメディクス，インコーポレイテッド 毒素および薬剤を含む治療用複合体
JP2005500808A (ja) * 2000-12-06 2005-01-13 ニユーララブ・リミテツド βアミロイドペプチドを認識するヒト化抗体
JP2006089471A (ja) * 2004-08-26 2006-04-06 National Institute Of Advanced Industrial & Technology 癌の治療における抗モータリン２抗体と機能性核酸の使用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BESPALOV I.A. ET AL.: "Recombinant Phabs reactive with 7,8-dihydro-8-oxoguanine, a major oxidative DNA lesion", BIOCHEMISTRY, vol. 35, no. 7, 1996, pages 2067 - 2078 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8586042B2 (en) 2009-12-10 2013-11-19 National Institute Of Advanced Industrial Science And Technology Hybridomas producing monoclonal anti-mortalin peptide antibodies
WO2011071099A1 (ja) * 2009-12-10 2011-06-16 独立行政法人産業技術総合研究所 抗癌性抗モータリンペプチド抗体
EP2511371A1 (en) * 2009-12-10 2012-10-17 National Institute of Advanced Industrial Science And Technology Anticancer anti-mortalin peptide antibodies
EP2511371A4 (en) * 2009-12-10 2013-05-15 Nat Inst Of Advanced Ind Scien PEPTIDANT BODY AGAINST CANCER AND MORTALINE
US9328160B2 (en) 2010-10-29 2016-05-03 Perseus Proteomics Inc. Anti-CDH3 antibody having high internalization capacity
WO2012057328A1 (ja) * 2010-10-29 2012-05-03 株式会社ペルセウスプロテオミクス 高い内在化能力を有する抗ｃｄｈ３抗体
JP2016175945A (ja) * 2010-10-29 2016-10-06 株式会社ペルセウスプロテオミクス 高い内在化能力を有する抗ｃｄｈ３抗体
JP6006640B2 (ja) * 2010-10-29 2016-10-12 株式会社ペルセウスプロテオミクス 高い内在化能力を有する抗ｃｄｈ３抗体
EP2700652A4 (en) * 2011-04-18 2015-07-15 Univ Tokyo DIAGNOSIS AND TREATMENT OF CANCER USING ANTI-ITM2A ANTIBODY
US9920129B2 (en) 2011-04-18 2018-03-20 The University Of Tokyo Diagnosis and treatment of cancer using anti-ITM2A antibody
EP3221350A4 (en) * 2014-11-20 2018-08-08 Ramot at Tel-Aviv University Ltd. Mortalin peptides and antibodies and uses thereof for inhibiting mortalin activity and treating a disease associated with a pathological cell
WO2017037707A1 (en) * 2015-09-02 2017-03-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Antibodies specific to human t-cell immunoglobulin and itim domain (tigit)
US10946095B2 (en) 2015-09-02 2021-03-16 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Antibodies specific to human T-cell immunoglobulin and ITIM domain (TIGIT)
US12121579B2 (en) 2015-09-02 2024-10-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Antibodies specific to human t-cell immunoglobulin and ITIM domain (TIGIT)

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