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WO2008143944A3 - Methods of glycosylation and bioconjugation - Google Patents

Methods of glycosylation and bioconjugation Download PDF

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Publication number
WO2008143944A3
WO2008143944A3 PCT/US2008/006248 US2008006248W WO2008143944A3 WO 2008143944 A3 WO2008143944 A3 WO 2008143944A3 US 2008006248 W US2008006248 W US 2008006248W WO 2008143944 A3 WO2008143944 A3 WO 2008143944A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
bioconjugation
glycosylation
galnac
provides
Prior art date
Application number
PCT/US2008/006248
Other languages
French (fr)
Other versions
WO2008143944A2 (en
Inventor
Pradman K Qasba
Boopathy Ramakrishnan
Original Assignee
Us Gov Health & Human Serv
Pradman K Qasba
Boopathy Ramakrishnan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Pradman K Qasba, Boopathy Ramakrishnan filed Critical Us Gov Health & Human Serv
Publication of WO2008143944A2 publication Critical patent/WO2008143944A2/en
Publication of WO2008143944A3 publication Critical patent/WO2008143944A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The instant invention provides isolated catalytic domains from a polypeptidyl-α- N-acetylgalactosaminyltransferase (pp-GalNAc-T). The invention provides methods for engineering a glycoprotein from a biological substrate, and methods for glycosylating a biological substrate for use in glycoconjugation. Also included in the invention are diagnostic and therapeutic uses. The invention further provides in vitro folding methods for pp-GalNAc-T.
PCT/US2008/006248 2007-05-14 2008-05-14 Methods of glycosylation and bioconjugation WO2008143944A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93029407P 2007-05-14 2007-05-14
US60/930,294 2007-05-14

Publications (2)

Publication Number Publication Date
WO2008143944A2 WO2008143944A2 (en) 2008-11-27
WO2008143944A3 true WO2008143944A3 (en) 2009-03-12

Family

ID=39807843

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/006248 WO2008143944A2 (en) 2007-05-14 2008-05-14 Methods of glycosylation and bioconjugation

Country Status (1)

Country Link
WO (1) WO2008143944A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009102820A2 (en) * 2008-02-11 2009-08-20 Government Of The U.S A., As Represented By The Secretary, Department Of Health And Human Services Modified sugar substrates and methods of use
EP3912642B1 (en) * 2012-10-23 2023-04-12 Synaffix B.V. Modified antibody, antibody-conjugate and process for the preparation thereof
PL2991683T3 (en) 2013-05-02 2020-03-31 Glykos Finland Oy Conjugates of a glycoprotein or a glycan with a toxic payload
US10905783B2 (en) 2015-11-27 2021-02-02 Michal RIVLIN Glucosamine and derivatives thereof in imaging
KR102291553B1 (en) * 2021-01-29 2021-08-18 씨제이제일제당 (주) Novel primosome assembly protein variant and a method for producing L-lysine using the same
EP4496587A1 (en) * 2022-03-21 2025-01-29 National Research Council of Canada Lipid based nanoparticles for targeted gene delivery to the brain
EP4496808A1 (en) * 2022-03-21 2025-01-29 National Research Council of Canada Antibodies with engineered o-glycosylation sequons for site-specific conjugation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005089102A2 (en) * 2004-02-04 2005-09-29 Neose Technologies, Inc. Methods of refolding mammalian glycosyltransferases
WO2005121331A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated galnact2 polypeptides and nucleic acids
WO2006102652A2 (en) * 2005-03-24 2006-09-28 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005089102A2 (en) * 2004-02-04 2005-09-29 Neose Technologies, Inc. Methods of refolding mammalian glycosyltransferases
WO2005121331A2 (en) * 2004-06-03 2005-12-22 Neose Technologies, Inc. Truncated galnact2 polypeptides and nucleic acids
WO2006102652A2 (en) * 2005-03-24 2006-09-28 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
QASBA PRADMAN K ET AL: "Mutant glycosyltransferases assist in the development of a targeted drug delivery system and contrast agents for MRI", AAPS JOURNAL, AMERICAN ASSOCIATION OF PHARMACEUTICAL SCIENTISTS, vol. 8, no. 1, 1 January 2006 (2006-01-01), pages E190 - E195, XP002491763, ISSN: 1550-7416, [retrieved on 20060324] *

Also Published As

Publication number Publication date
WO2008143944A2 (en) 2008-11-27

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