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WO2008143159A1 - Method and kit for measurement of cholesterol in low-density lipoprotein - Google Patents

Method and kit for measurement of cholesterol in low-density lipoprotein Download PDF

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Publication number
WO2008143159A1
WO2008143159A1 PCT/JP2008/059006 JP2008059006W WO2008143159A1 WO 2008143159 A1 WO2008143159 A1 WO 2008143159A1 JP 2008059006 W JP2008059006 W JP 2008059006W WO 2008143159 A1 WO2008143159 A1 WO 2008143159A1
Authority
WO
WIPO (PCT)
Prior art keywords
cholesterol
reaction
kit
measurement
low
Prior art date
Application number
PCT/JP2008/059006
Other languages
French (fr)
Japanese (ja)
Inventor
Tomomi Murakami
Original Assignee
Kyowa Medex Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Medex Co., Ltd. filed Critical Kyowa Medex Co., Ltd.
Priority to JP2009515201A priority Critical patent/JP5534809B2/en
Publication of WO2008143159A1 publication Critical patent/WO2008143159A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Disclosed is a method for measuring LDL cholesterol contained in a sample, which comprises the steps of: reacting the sample containing an LDL with a cholesterol ester hydrolase in an aqueous medium free of a cholesterol oxidase; allowing a specific surfactant such as a polyoxyethylene (POE) branched alkyl ether and a cholesterol oxidase to co-exist in the reaction solution to cause the reaction between cholesterol in the reaction solution with the cholesterol oxidase and, if required, the cholesterol ester hydrolase; and measuring a substance produced by the reaction or a substance consumed by the reaction. Also disclosed is a kit for use in the method.
PCT/JP2008/059006 2007-05-18 2008-05-16 Method and kit for measurement of cholesterol in low-density lipoprotein WO2008143159A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009515201A JP5534809B2 (en) 2007-05-18 2008-05-16 Method and kit for measuring cholesterol in low density lipoprotein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007132862 2007-05-18
JP2007-132862 2007-05-18

Publications (1)

Publication Number Publication Date
WO2008143159A1 true WO2008143159A1 (en) 2008-11-27

Family

ID=40031861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059006 WO2008143159A1 (en) 2007-05-18 2008-05-16 Method and kit for measurement of cholesterol in low-density lipoprotein

Country Status (2)

Country Link
JP (1) JP5534809B2 (en)
WO (1) WO2008143159A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012057333A1 (en) * 2010-10-29 2012-05-03 アークレイ株式会社 Method and kit for measuring cholesterol in low density lipoproteins
JPWO2011136316A1 (en) * 2010-04-30 2013-07-22 協和メデックス株式会社 Method for measuring cholesterol in low density lipoprotein, reagent for measurement and kit for measurement
JP2015134159A (en) * 2013-12-16 2015-07-27 国立研究開発法人国立循環器病研究センター Artificial blood vessel and method for manufacturing artificial blood vessel

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996028734A1 (en) * 1995-03-16 1996-09-19 Kyowa Medex Co., Ltd. Method of quantitating cholesterol in low-density lipoprotein
WO1996029599A1 (en) * 1995-03-20 1996-09-26 Kyowa Medex Co., Ltd. Method of quantifying cholesterol in low-density or very-low-density lipoprotein
WO2000017388A1 (en) * 1998-09-18 2000-03-30 Kyowa Medex Co., Ltd. Methods for fractional quantification of cholesterol in lipoproteins and quantification reagents
WO2003104486A1 (en) * 2002-06-10 2003-12-18 株式会社シノテスト Method of selectively measuring triglycerides
WO2006085654A1 (en) * 2005-02-14 2006-08-17 Kyowa Medex Co., Ltd. Method, reagent and kit for quantifying cholesterol in remnant-like lipoprotein (rlp)
WO2006118199A1 (en) * 2005-04-27 2006-11-09 Kyowa Medex Co., Ltd. Method for determination of cholesterol in high-density lipoprotein

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996028734A1 (en) * 1995-03-16 1996-09-19 Kyowa Medex Co., Ltd. Method of quantitating cholesterol in low-density lipoprotein
WO1996029599A1 (en) * 1995-03-20 1996-09-26 Kyowa Medex Co., Ltd. Method of quantifying cholesterol in low-density or very-low-density lipoprotein
WO2000017388A1 (en) * 1998-09-18 2000-03-30 Kyowa Medex Co., Ltd. Methods for fractional quantification of cholesterol in lipoproteins and quantification reagents
WO2003104486A1 (en) * 2002-06-10 2003-12-18 株式会社シノテスト Method of selectively measuring triglycerides
WO2006085654A1 (en) * 2005-02-14 2006-08-17 Kyowa Medex Co., Ltd. Method, reagent and kit for quantifying cholesterol in remnant-like lipoprotein (rlp)
WO2006118199A1 (en) * 2005-04-27 2006-11-09 Kyowa Medex Co., Ltd. Method for determination of cholesterol in high-density lipoprotein

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2011136316A1 (en) * 2010-04-30 2013-07-22 協和メデックス株式会社 Method for measuring cholesterol in low density lipoprotein, reagent for measurement and kit for measurement
JP5832995B2 (en) * 2010-04-30 2015-12-16 協和メデックス株式会社 Method for measuring cholesterol in low density lipoprotein, reagent for measurement and kit for measurement
WO2012057333A1 (en) * 2010-10-29 2012-05-03 アークレイ株式会社 Method and kit for measuring cholesterol in low density lipoproteins
JP5728490B2 (en) * 2010-10-29 2015-06-03 アークレイ株式会社 Method and kit for measuring cholesterol in low density lipoprotein
JP2015134159A (en) * 2013-12-16 2015-07-27 国立研究開発法人国立循環器病研究センター Artificial blood vessel and method for manufacturing artificial blood vessel

Also Published As

Publication number Publication date
JP5534809B2 (en) 2014-07-02
JPWO2008143159A1 (en) 2010-08-05

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