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WO2008143047A1 - 樹状細胞の製造方法 - Google Patents

樹状細胞の製造方法 Download PDF

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Publication number
WO2008143047A1
WO2008143047A1 PCT/JP2008/058719 JP2008058719W WO2008143047A1 WO 2008143047 A1 WO2008143047 A1 WO 2008143047A1 JP 2008058719 W JP2008058719 W JP 2008058719W WO 2008143047 A1 WO2008143047 A1 WO 2008143047A1
Authority
WO
WIPO (PCT)
Prior art keywords
dendritic cell
precursor cell
production
produce
large quantity
Prior art date
Application number
PCT/JP2008/058719
Other languages
English (en)
French (fr)
Inventor
Makoto Inoue
Mamoru Hasegawa
Yoshikazu Yonemitsu
Yui Harada
Original Assignee
Dnavec Corporation
Ueda, Yasuji
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dnavec Corporation, Ueda, Yasuji filed Critical Dnavec Corporation
Priority to CN200880025104.0A priority Critical patent/CN101755045B/zh
Priority to EP08752599.4A priority patent/EP2154243B1/en
Priority to KR1020097026104A priority patent/KR101506751B1/ko
Priority to JP2009515156A priority patent/JP5227317B2/ja
Priority to US12/600,103 priority patent/US8283163B2/en
Priority to CA2687534A priority patent/CA2687534A1/en
Publication of WO2008143047A1 publication Critical patent/WO2008143047A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0639—Dendritic cells, e.g. Langherhans cells in the epidermis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/19—Dendritic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/24—Antigen-presenting cells [APC]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/428—Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterized by the route of administration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

本発明は、複数のサイトカインの存在下でDC前駆細胞を培養する工程を含む、DCの製造方法、製造された樹状細胞およびその利用を提供する。本発明の方法は、DCへの高い分化能を持つ大量のDC前駆体を製造することを可能にする。本発明により、少ないDC前駆細胞から大量のDCを得ることが可能となるので、DCを用いた抗腫瘍免疫療法および感染症治療などにおいて投与するDC数を増やすことが容易になり、DCワクチンの効果の増大が期待される。
PCT/JP2008/058719 2007-05-17 2008-05-12 樹状細胞の製造方法 WO2008143047A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN200880025104.0A CN101755045B (zh) 2007-05-17 2008-05-12 树突状细胞的制备方法
EP08752599.4A EP2154243B1 (en) 2007-05-17 2008-05-12 Method for production of dendritic cell
KR1020097026104A KR101506751B1 (ko) 2007-05-17 2008-05-12 수상세포의 제조방법
JP2009515156A JP5227317B2 (ja) 2007-05-17 2008-05-12 樹状細胞の製造方法
US12/600,103 US8283163B2 (en) 2007-05-17 2008-05-12 Method for production of dendritic cell
CA2687534A CA2687534A1 (en) 2007-05-17 2008-05-12 Method for production of dendritic cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-132204 2007-05-17
JP2007132204 2007-05-17

Publications (1)

Publication Number Publication Date
WO2008143047A1 true WO2008143047A1 (ja) 2008-11-27

Family

ID=40031756

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/058719 WO2008143047A1 (ja) 2007-05-17 2008-05-12 樹状細胞の製造方法

Country Status (7)

Country Link
US (1) US8283163B2 (ja)
EP (1) EP2154243B1 (ja)
JP (1) JP5227317B2 (ja)
KR (1) KR101506751B1 (ja)
CN (1) CN101755045B (ja)
CA (1) CA2687534A1 (ja)
WO (1) WO2008143047A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102154199A (zh) * 2011-01-29 2011-08-17 暨南大学 一种半成熟树突状细胞的体外制备方法
JP2011239701A (ja) * 2010-05-14 2011-12-01 Tella Inc 樹状細胞の培養方法
US8741639B2 (en) 2008-11-14 2014-06-03 Dnavec Corporation Method for producing dendritic cells
JP2014111631A (ja) * 2014-01-31 2014-06-19 Tella Inc 樹状細胞の培養方法
JP2016094458A (ja) * 2015-12-24 2016-05-26 テラ株式会社 樹状細胞の培養方法
JP2017110023A (ja) * 2017-02-01 2017-06-22 株式会社ガイアバイオメディシン 樹状細胞の培養方法
JPWO2017065288A1 (ja) * 2015-10-15 2018-08-09 国立大学法人京都大学 多能性幹細胞からｃｄ４陽性ｔ細胞を製造する方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2544786A1 (en) * 2003-11-04 2005-05-12 Dnavec Research Inc. Method for producing gene transferred dendritic cells
KR20070028573A (ko) * 2004-06-24 2007-03-12 가부시키가이샤 디나벡크 겐큐쇼 마이너스 가닥 ｒｎａ 바이러스를 포함하는 항암제
CN102596234B (zh) * 2009-07-24 2016-11-16 罗得岛医院 针对表达天冬酰胺酰-β-羟化酶的肿瘤的树突细胞疫苗
WO2013078059A1 (en) * 2011-11-22 2013-05-30 Trustees Of Tufts College Small molecule enhancer for dendritic cell cancer vaccines
ES2617510T3 (es) 2012-04-26 2017-06-19 Riken Nuevo carbamato de glicolípido y uso del mismo
US10849985B2 (en) 2015-05-20 2020-12-01 Yeda Research And Development Co. Ltd. Method of targeting senescent cells

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US5811297A (en) 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
IL127912A0 (en) 1996-07-10 1999-11-30 Immunex Corp Method of activating dendritic cells
CN1408850A (zh) * 2001-09-18 2003-04-09 王虹 人血树突状细胞永生化技术
IL161832A0 (en) 2001-11-29 2005-11-20 Dandrit Biotech As Pharmaceutical composition for inducing an immune response in a human or animal
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
CA2544786A1 (en) 2003-11-04 2005-05-12 Dnavec Research Inc. Method for producing gene transferred dendritic cells
KR20070028573A (ko) 2004-06-24 2007-03-12 가부시키가이샤 디나벡크 겐큐쇼 마이너스 가닥 ｒｎａ 바이러스를 포함하는 항암제
KR102116658B1 (ko) * 2008-03-27 2020-06-01 아스테리아스 바이오세라퓨틱스, 인크. 영장류 다능성 줄기 세포의 조혈계 세포로의 분화

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BONTKES H.J. ET AL.: "Expansion of dendritic cell precursors from human CD34(+) progenitor cells isolated from healthy donor blood; growth factor combination determines proliferation rate and functional outcome", J. LEUKOC. BIOL., vol. 72, no. 2, 2002, pages 321 - 329, XP002990425 *
SARAYA K. ET AL.: "Synergistic interaction between c-kit ligand (SCF), GM-CSF and TNF promotes optimal dendritic Langerhans cell proliferation from primitive progenitors in human bone marrow", ADV. EXP. MED. BIOL., vol. 378, 1995, pages 13 - 16, XP008124841 *
See also references of EP2154243A4 *
SZABOLCS P. ET AL.: "Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c-kit ligand, granulocyte-macrophage colony-stimulating factor, and TNF-alpha", J. IMMUNOL., vol. 154, no. 11, 1995, pages 5851 - 5861, XP001179701 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8741639B2 (en) 2008-11-14 2014-06-03 Dnavec Corporation Method for producing dendritic cells
JP2011239701A (ja) * 2010-05-14 2011-12-01 Tella Inc 樹状細胞の培養方法
CN102154199A (zh) * 2011-01-29 2011-08-17 暨南大学 一种半成熟树突状细胞的体外制备方法
JP2014111631A (ja) * 2014-01-31 2014-06-19 Tella Inc 樹状細胞の培養方法
JPWO2017065288A1 (ja) * 2015-10-15 2018-08-09 国立大学法人京都大学 多能性幹細胞からｃｄ４陽性ｔ細胞を製造する方法
JP7017008B2 (ja) 2015-10-15 2022-02-08 国立大学法人京都大学 多能性幹細胞からｃｄ４陽性ｔ細胞を製造する方法
US11987811B2 (en) 2015-10-15 2024-05-21 Kyoto University Method for producing CD4-positive T cells from pluripotent stem cells
JP2016094458A (ja) * 2015-12-24 2016-05-26 テラ株式会社 樹状細胞の培養方法
JP2017110023A (ja) * 2017-02-01 2017-06-22 株式会社ガイアバイオメディシン 樹状細胞の培養方法

Also Published As

Publication number Publication date
KR20100035634A (ko) 2010-04-05
EP2154243A1 (en) 2010-02-17
CN101755045B (zh) 2014-02-12
JPWO2008143047A1 (ja) 2010-08-05
JP5227317B2 (ja) 2013-07-03
CN101755045A (zh) 2010-06-23
CA2687534A1 (en) 2008-11-27
EP2154243A4 (en) 2011-07-06
US8283163B2 (en) 2012-10-09
EP2154243B1 (en) 2016-01-13
US20100184214A1 (en) 2010-07-22
KR101506751B1 (ko) 2015-03-27

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