WO2008035572A1

WO2008035572A1 - Moisturizing agent, anti-aging agent, skin-whitening agent, antiinflammatory agent, and antioxidant agent

Info

Publication number: WO2008035572A1
Application number: PCT/JP2007/067415
Authority: WO
Inventors: Masaki Arashima; Yoko Asano; Hiroko Yoshida; Akira Hatani
Original assignee: Noevir Co., Ltd.
Priority date: 2006-09-19
Filing date: 2007-09-06
Publication date: 2008-03-27
Also published as: JP2008074730A; JP5025201B2

Abstract

Disclosed are a moisturizing agent, an anti-aging agent, a skin-whitening agent, an anti-inflammatory agent and an antioxidant agent, each of which comprises a plant belonging to the genus Barringtonia or Couroupita or an extract thereof as an active ingredient. Also disclosed is a composition comprising a plant belonging to the genus Barringtonia or Couroupita or an extract thereof.

Description

Specification

Moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, and antioxidants

Technical field

[0001] The present invention relates to a moisturizer, an anti-aging agent, a whitening agent, and an anti-inflammatory agent comprising a naturally-derived component as an active ingredient.

, And antioxidants. More specifically, the present invention relates to the use of the genus Sagaribana, the genus Botanus, or an extract thereof.

Background art

[0002] As skin aging decreases with age, aging symptoms such as wrinkles and blemishes cause cell function decline, decrease or degeneration of extracellular matrix components such as collagen, melanin production and pigmentation due to ultraviolet rays, and cells Oxidative damage and the like. In order to prevent and improve such aging symptoms, various active ingredients have been searched and compounded.

[0003] As the cell activator, the essence of Ponkan (JP 2001-131045), as the collagenase inhibitor, dicarboxylic acid (JP 9-124472), as the collagen production promoter, beechaceae. Extracts from tree buds of beech plants (Japanese Patent Laid-Open No. 10-203952), whitening agents include water and / or organic solvent extracts of white crane reishi (Japanese Patent Laid-Open No. 2003-89630), as antioxidants Extract of plants belonging to the genus Sarogase (Japanese Patent Laid-open No. 10-182413), tea polyphenols (Japanese Patent Laid-Open No. 6-9391) as anti-inflammatory agents, and chlorella as an aromatase activity promoter. An extract (Japanese Patent Laid-Open No. 2004-186099) is known.

Disclosure of the invention

[0004] Thus, various naturally-derived components have been applied so far. There are many natural ingredients that are not yet known for their effects, and have excellent moisturizing, anti-aging, whitening, anti-inflammatory, or antioxidant effects. The development of active ingredients was expected.

The present invention has developed an active ingredient having an excellent moisturizing action, anti-aging action, whitening action, anti-inflammatory action, or anti-oxidation action, and has a moisturizing agent, anti-aging agent, whitening agent, anti-inflammation having those effects. It is an object to provide an agent, an antioxidant, and various compositions. [0005] As a result of studying various components derived from nature, the present inventors have found that moisturizing action, anti-aging action, and whitening, which are superior to the genus Sagaribana or the genus Botanus, whose effects have not been known. The present inventors have found that there are an action, an anti-inflammatory action, and an anti-oxidant action, and have further studied to complete the present invention.

That is, according to the first aspect of the present invention, a moisturizer, an anti-aging agent, a whitening agent comprising one or more plants selected from the genus Sagaribana or the plant of the genus Botanus or an extract thereof as an active ingredient, The present invention relates to an anti-inflammatory agent and an antioxidant.

According to a second aspect of the present invention, there is provided a composition comprising a genus Sagaribana or a genus Botanus, or an extract thereof.

BEST MODE FOR CARRYING OUT THE INVENTION

[0006] The plant used as the raw material of the present invention is the Sagaribana family that is an evergreen or deciduous tree.

(Lecvthidaceae) Plants of the genus Barringtonia or the evergreen or deciduous tree, the genus Couroupita! /. Examples of the genus Sagaribana are Sagarinona (Barringtonia racemosa) and Kononnoashi (Barringtonia asiatica). As a plant belonging to the genus Vulgaris, Vulcanum (Couro upita guianensis j) has been lost.

The genus Sagaribana or the genus Botanus, or an extract thereof, contains an extremely large number of components that cannot be analyzed, and these act collectively to obtain the various effects of the present invention. It is estimated that

[0007] The plant used as a raw material is not particularly limited as long as it is a plant belonging to the genus Sagaribana or the genus Boletus, but bar ringtonia asiatica may be used because of its relative availability and effectiveness. In particular, it is also possible to use a combination of a plurality of species of the genus Sagaribana or the genus Botanus.

[0008] When using this plant of the genus Sagaribana or the genus Botanus, there are no particular restrictions on the site of use, or flowers, leaves, stems, branches, roots, seeds, bark, sap, pericarp, fruits, buds, etc. Any site can be used. Multiple parts may be used in combination. For convenient use, the use of leaves, stems, and seeds is preferable from the viewpoint of effectiveness. It is preferable to pulverize them as they are, and to use the raw material or the dried product, and use an extract from those parts.

[0009] For extraction, any part of the plant belonging to the genus Sagaribana or the genus Botanus may be used, but for easy use, leaves, stems, seeds, etc. may be used. At that time, an extract may be obtained using a plurality of parts. You can also use a mixture of two or more extracts extracted with different solvents.

In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to carry out the extraction after processing such as shredding, drying, and grinding.

[0010] Extraction methods include, for example, a method of extraction by immersing in an arbitrary extraction solvent for a predetermined time in a cooled or heated state at room temperature, a method of extraction using a distillation method such as steam distillation, or a raw plant Examples of the pressing method include pressing to obtain an extract. Any of these methods can be used alone or in combination of two or more. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be stirred or homogenized in an extraction solvent. It is also possible to extract under a caloric pressure using an autoclave.

[0011] The extraction temperature is suitably about 5 ° C to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it for about 1 hour to 14 days.

The ratio of the plant and the solvent during the extraction is not particularly limited, but is preferably 0.5 to 5 times the solvent for the plant 1; Preferably 100 times by mass.

[0012] Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin; ethyl ether, propyl ether Solvents such as ethers such as butyl acetate, butyl acetate, ethyl acetate, and the like; ketones such as acetone, ethylmethylol ketone, and the like can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline and the like may be used. In addition, water, carbon dioxide, ethylene, propylene One or more supercritical liquids and subcritical liquids such as hydrogen, ethanol, methanol, and ammonia may be used.

[0013] The above-mentioned solvent extract of the genus Sagaribana or the genus Botanus can be used as it is. It may be used after aging for a certain period of time, or the concentrated and dried product may be used as water or polar. It can also be dissolved in a solvent and used. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., or fractionation treatment by column chromatography etc., as long as these physiological effects are not impaired. The above-mentioned extract of Sagaribana plant or Botanus genus plant or its treated product and fractionated product can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use. It can also be used by being encapsulated in vesicles such as ribosomes or microcapsules.

[0014] The plant of the genus Sagaribana or the genus Botanus, or an extract thereof, has an excellent moisturizing action, anti-aging action, whitening action, anti-inflammatory action, and anti-oxidation action, and is a moisturizing agent, anti-aging agent, whitening agent. It can be preferably used as an anti-inflammatory agent or an antioxidant. Each of these agents is not limited at all in terms of its form and the presence or absence of other ingredients, as long as it contains a sorghum plant or a genus genus plant, or an extract thereof as an active ingredient. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use etc., and the vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

[0015] The amount of the active ingredient in each agent, the plant of the genus Sagaribana, the plant of the genus Botanus, or the extract thereof can be adjusted according to the type of agent, the purpose of use, etc. , based on the total amount, 0.1 in terms of solid content 00001～; 100 wt% is laid preferred, more preferably 0.00; is ~ 50 mass ^_0/0!.

[0016] A moisturizing agent containing a plant belonging to the genus Sagaribana or Botanus spp. Or an extract thereof as an active ingredient exhibits an excellent moisturizing action on the skin and hair, and has a particularly high moisturizing effect on the skin.

[0017] An anti-aging agent comprising a plant of the genus Sagaribana or Botanus genus or an extract thereof as an active ingredient has an excellent cell activation effect, collagen production activity, and aromatase activity. Has an accelerating action and exhibits an excellent effect in preventing and improving aging symptoms. Aromatase is an enzyme that works when producing estrogen. If the production of estrogen is promoted by promoting aromatase activity, the skin-beautifying effect of female hormones can be expected to have an anti-aging effect.

[0018] A whitening agent containing a plant of the genus Sagaribana or Botanus, or an extract thereof, as an active ingredient is effective in improving pigmentation symptoms such as stains and buckwheat, particularly inhibiting tyrosinase activity and suppressing melanin production. Excellent effect on

[0019] An anti-inflammatory agent comprising a genus Sagaribana or a genus Botanus or an extract thereof as an active ingredient has an excellent hyaluronidase inhibitory effect and suppresses skin inflammation and exhibits an excellent anti-inflammatory action.

[0020] Antioxidants containing Sagaribana plants or genus plants, or extracts thereof, as active ingredients have excellent free radical scavenging effects and superoxide anion scavenging effects, such as photoaging of the skin. Prevents and exhibits an excellent antioxidant effect.

[0021] Each of these agents can be used for hair and the like as well as applied externally to the skin, and can be applied to various compositions such as an external composition and an oral composition. Here, the composition for external use is not limited to any one category such as cosmetics, external preparations for skin, quasi-drugs, external medicines, etc., but any combination for external use on skin or hair. Means an adult. The term “oral composition” means any composition that can be taken orally, regardless of the type of drug, food, beverage, etc.

[0022] The dosage form of the composition for external use is arbitrary, and can be provided, for example, as a solubilization system such as lotion, a dispersion system such as calamine mouth lotion, or an emulsification system such as cream or emulsion. In addition, it can be provided in various dosage forms such as aerosol forms, ointments, and poultices filled with propellants.

Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents, lipsticks, makeup cosmetics, foundations, etc .; liquids, ointments, powders, granules, aerosols, patching agents Examples include various forms of quasi-drugs such as cataplasms and cataplasms for external use. [0023] The form of the oral composition is also arbitrary, and is in a liquid form such as a liquid, syrup, or extract, or a solid preparation such as a granule, tablet, powder, capsule, or glaze, or a jelly, It can be processed and used in various forms such as gummi and gum, and is not particularly limited.

Specific examples include general foods including beverages, health foods (supplements), functional foods, nutritional supplements, oral medicines, and quasi drugs.

[0024] By blending the plant of the genus Sagaribana or the genus Botanus, or an extract thereof, with a topical skin preparation including, for example, cosmetics, topical medicines, quasi drugs, etc. Prevents various skin symptoms such as reduced skin elasticity, spots, dullness, dryness, fine lines, etc.Preparation of an external composition that exhibits an excellent effect for improvement, and as a skin moisturizing or whitening skin external preparation Can be used. In particular, it is preferably used as a skin external preparation for improving aging prevention, which contains a plant belonging to the genus Sagaribana or the genus Botanus, or an extract thereof.

In addition, Sagaribana or Hoganae plants, or extracts thereof, are used for beauty and health maintenance such as whitening, health foods (supplements), quasi-drugs, functional products. It can also be used for foods.

[0025] Compositions for external use or oral use include, for example, sagaribana plants, or Astragalus plants, or extracts thereof, as well as normal skin cosmetics, hair, if necessary. This includes optional ingredients used in pharmaceutical preparations such as cosmetics, quasi drugs, and pharmaceuticals. Such components include water, oils (oil-based ingredients), alcohols, excipients, binders, extenders, disintegrants, corrigents, dyes, colorants, emulsifiers, solubilizers, dispersants, gelling. Agent, plasticizer, cleaning agent, UV absorber, thickener, pH adjuster, buffer, surfactant, lubricant, chelating agent, drug (medicinal ingredient), fragrance, resin, coating agent, antibacterial Examples include glazes, preservatives, preservatives, antioxidants, and pH adjusters. In addition, other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, antioxidants, plants other than Sagaribana plants or Hoganae plants, or extracts thereof, as long as the effects of the present invention are not impaired. Combination with and is also possible.

[0026] Even in the case of foods and drinks containing Sagaribana plants or Botanus plants, or extracts thereof, those that are particularly limited in combination with various ingredients used in foods Absent.

In other words, the dosage form of food is arbitrary, and can be provided in various dosage forms such as powders, granules, capsules, liquids, etc. Oil components, moisturizers, powders, emulsifiers, solubilizers, thickeners, drugs, fragrances, antifungal agents, alcohols, sugar, condensed milk, flour, salt, glucose, chicken eggs, butter, marga Phosphorus, chickenpox, calcium, iron, seasonings, spices, vitamin A and their derivatives, strength rotenoids, riboflavin and its derivatives, vitamin B and its salts or derivatives, ascorbic acid and its derivatives, cobalamins, vitamin E And their derivatives, vitamins, adenosine and its derivatives, flavonoids and tannins. Furthermore, other moisturizers, anti-aging agents, whitening agents, anti-inflammatory agents, or antioxidants can be used in combination as long as the effects of the present invention are not impaired.

[0027] The blending amount of the genus Sagaribana or the genus Botanus in the composition for external use or the composition for oral use, or the extract thereof is adjusted according to the kind of composition, the purpose of use, etc. in view of the force effect and stability it can, based on the total amount, preferably from 0. 00001- 50.0 mass ^_0/0 power Mashigu in terms of solid content <is 0. 0001- 25.0 mass ^_0/0. Further, 0.1 0001～; 10 mass ^_0/0 Ca, more preferably 0.000; a ~ 5 mass ^_0/0, more preferably 0. 00;! A ~ 5 wt%, more preferably Is in the range of 0.0;! To 5 mass%, particularly preferably 0;

Example

[0028] In the following, the ability to explain in detail the production examples of extracts of the genus Sagaribana or Botanus, tests for evaluating each action, formulation examples for skin external preparations and foods, and use tests The technical scope is not limited at all by this.

[0029] <Extraction method;!>

The leaf of Covanno reed or Astragalus was dried and pulverized, 50% ethanol by mass of 20 times the mass of the sample was added, and the mixture was extracted with stirring at room temperature for 2 hours. The obtained extract was filtered to remove insoluble matters, concentrated under reduced pressure, and then freeze-dried to obtain each extract of Copano reed or Hogan.

[0030] <Extraction method 2> The leaves of Copin's reed or scallop were dried and pulverized, purified water of 20 times the sample mass was added, and the mixture was extracted by heating to 120 ° C for 20 minutes using an autoclave. From the obtained extract, insolubles were removed by suction filtration while maintaining a high temperature state, and then freeze-dried to obtain each extract of Copano reed or Hogan.

[0031] <Evaluation of dermal fibroblast activation effect>

Using the ethanol extract obtained by the extraction method 1 as a sample, the dermal fibroblast activation action was evaluated as follows.

[0032] Normal human dermal fibroblasts manufactured by Kurashiki Boseki Co., Ltd. were seeded in a 96-well microphone mouth plate so that 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight urchin fetal serum (FBS). After culturing for 24 hours, the sample culture medium was adjusted to each sample concentration shown in Table 1 with 1 mass ° / 83-added DMEM medium, and further cultured for 48 hours. After removing the supernatant, the medium was replaced with a medium containing 400 μg / ml of 3- (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide (MTT reagent) and cultured for about 2 hours. . Then, the formazan produced by the opening of the tetrazolium ring was extracted with 2 propanol, and the absorbance at 55 Onm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

[0033] The results obtained are shown in Table 1 as relative values when the cell activation effect in the control without addition of the sample is taken as 100.

[0034] [Table 1]

[0035] As is clear from Table 1, in the medium to which the sample was added, a significant dermal fibroblast activation effect was observed.

Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal cell activation effect was evaluated as follows. [0037] Human epidermal non-keratinized cells were seeded in a 96-well microphone mouth plate so that the number of cells was 2.0 × 10 ⁴ per wellore. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight urchin fetal serum (FBS). After culturing for 24 hours, the sample culture medium was adjusted to the sample concentration shown in Table 2 with 5 mass ° / ^ 8 S-added DMEM medium, and further cultured for 24 hours. After removing the supernatant, the MTT reagent was replaced with a medium containing lOO ^ g / ml and cultured for about 2 hours. Subsequently, fonolemazan produced by the opening of the tetrazolium ring was extracted with 2 propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

[0038] The results obtained are shown in Table 2 by relative values when the cell activation effect in the control without addition of the sample is taken as 100.

[0039] [Table 2]

[0040] As is clear from Table 2, a significant epidermal cell activation effect was observed in the medium to which the sample was added.

[0041] <Evaluation of dermal fibroblast collagen production>

Using the ethanol extract and hot water extract obtained by the extraction methods 1 and 2 as samples, the dermal fibroblast collagen production action was evaluated as follows.

[0042] Normal human dermal fibroblasts were seeded on a 96-well microphone plate at 2.0 x 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight urine fetal serum (FBS). After culturing for 24 hours, the medium was replaced with a culture medium adjusted to the sample concentration shown in Table 3 with 0.5% by mass FBS-added DMEM medium, and further cultured for 24 hours.

[0043] An ELISA method was used to quantify type I and type III collagen secreted into the culture supernatant, and finally, 2, 2 'azinobis (3 ethylbennes) against labeled peroxidase. Zothiazoline-6-sulfonic acid) diammonium salt (ABTS) and hydrogen peroxide were added and reacted, and the absorbance at 405 nm was measured with a microplate reader.

The amount of protein was measured by BIER Protein Assay Kit manufactured by PIERCE, and the amount of type I and type III collagen produced per unit protein was determined.

[0044] The results obtained are shown as the type per unit protein in the control with no sample added.

Table 3 shows the relative values when the amount of collagen production of type I and type III is 100.

[0045] [Table 3]

[0046] As is clear from Table 3, in the medium to which the sample was added, a significant dermal fibroblast collagen production effect was observed.

<0047> <Evaluation of epidermal cell collagen production>

Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal cell collagen production action was evaluated as follows.

[0048] Non-keratinized human epidermis cells were seeded in a 96-well microphone mouth plate so that there were 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight urine fetal serum (FBS). After culturing for 24 hours, the sample was replaced with a Sampnore culture solution adjusted to each sample concentration shown in Table 4 with a DMEM medium containing 5 mass ° / (^ 8 S, and further cultured for 5 days.

[0049] For the quantification of type IV collagen secreted into the culture supernatant, sandwich ELIS screening method using monoclonal antibody against IV collagen (recognition site: _α 2 chain) and biotinylated polyclonal antibody was used. Horseradish peroxidase was added, the color was developed with 3,3 ', 5,5'-tetramethylbenzidine, and the absorbance at 650 nm was measured with a microplate reader.

The protein amount is measured by PIERCE BCA Protein Assay Kit The amount of type IV collagen produced per unit amount was determined.

[0050] The results obtained are the types per unit protein amount in the control with no sample added.

Table 4 shows the relative values when the amount of IV collagen produced is 100.

[0051] [Table 4]

[0052] As is apparent from Table 4, in the medium to which the sample was added, a significant epidermal cell collagen production effect was observed.

[0053] <Evaluation of aromatase activity promoting action>

Using the ethanol extract obtained by the above extraction method 1 as a sample, the aromatase activity promoting action was evaluated as follows.

[0054] NADP +, MgCl, Darco was added to 4 μ 1 of the sample solution prepared for each sample concentration shown in Table 5.

2 Mixed solution of 6-phosphate, glucose 6-phosphate dehydrogenase, and control insect cell membrane protein (CPY19 / MFC High Throughput Inhibitor Screening Kit), manufactured by BD Biosciences 96 1 was added and warmed to 37 ° C for 10 minutes. 15 nM CPY19 (alomatase), 50 M 7 methoxy-l 4 trifluoromethylcoumarin (substrate) solution 100 1 were added, and the mixture was heated to 37 ° C for 30 minutes. lOOmM Tris base 75 1 was added to stop the reaction.

[0055] Fluorescence measurement was performed at an excitation wavelength of 409 nm and an emission wavelength of 530 nm. Since 7-methoxy-4-trifluoromethylcoumarin is decomposed by CYP19 and 7-hydroxy-4-trifluoromethylcoumarin is produced to produce fluorescence, the ability to promote aromatase activity was quantified by fluorescence measurement.

[0056] The results obtained are based on the aromatase activity promoting effect in the control without addition of the sample.

Table 5 shows the relative values when 100 is assumed.

[0057] [Table 5] Coban Noashi (leaves)

Sample addition concentration (mg / ml)

% of contro l

Control 100

0. 13 1 1 6

0. 25 1 25

[0058] As is apparent from Table 5, when the sample was added, a significant aromatase activity promoting action was observed.

[0059] <Evaluation of inhibitory action on epidermal melanocyte tyrosinase activity>

Using the ethanol extract obtained by the extraction method 1 as a sample, the epidermal melanocyte tyrosinase activity inhibitory action was evaluated as follows.

[0060] Normal human epidermal melanocytes were seeded on a 96-well microphone plate so that there were 3.0 x 10 ⁴ cells per well. As the seeding medium, Medium 154S manufactured by Kurashiki Boseki Co., Ltd. was used. 24 After culturing for 4 hours, the medium was replaced with a sample culture solution adjusted to each sample concentration shown in Table 6 with Medium 154S, and further cultured for 48 hours. Next, the cells were completely lysed by exchanging with phosphate buffer 751 containing 1% by mass 1¾101-X, and 501 of which was used as a crude enzyme solution. To the crude enzyme solution, 0.05 mass% L-dopa-containing phosphate buffer 501, which serves as a substrate, was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after substrate addition and at the end of the reaction with a microplate reader, and each measured value was introduced into the following equation to determine the amount of produced dopamelanin.

[0061] Dopamelanin production =

{(Post-reaction 405 value—Before reaction 405 stomach value) — 2. 166} / 5. 238

In addition, the amount of protein was measured by BIER Protein Assay Kit manufactured by PIERCE, and the amount of melanin produced per unit protein amount was determined. Based on the melanin production obtained, the inhibition rate for the case of no sample was calculated, and the results are shown in Table 6.

[0062] [Table 6]

[0063] As is clear from Table 6, in the medium to which the sample was added, a decrease in melanin production was observed. An excellent inhibitory effect on tyrosinase activity was observed.

[0064] <Evaluation of Hyaluronidase Inhibitory Action>

Using the ethanol extract obtained by the above extraction method 1 as a sample, the hyaluronidase inhibitory action was evaluated as follows.

[0065] Commercially available hyaluronic acid potassium salt (derived from human umbilical cord) was dissolved in 0.1 M phosphate buffer (ρΗ7.0) to a concentration of 0.9 mg / ml to obtain a substrate solution. A commercially available hyaluronidase (derived from urchin testis) was dissolved in 0.1 M phosphate buffer (pH 7.0) so as to be 5,300 unit / ml to obtain an enzyme solution. The enzyme solution was prepared at the time of use.

[0066] 0.1 ml of a solution prepared with a buffer solution at each sample concentration shown in Table 7 and 0.03 ml of enzyme solution were placed in a test tube and reacted at 37 ° C for 20 minutes. Next, 0.06 ml of activator was added and reacted at 37 ° C for 20 minutes. Further, 0.15 ml of the substrate solution was added and reacted at 37 ° C for 1 hour. After stopping the reaction by adding 0.06 ml of 4N NaOH aqueous solution, immediately cool on ice, add 0.06 ml of folate buffer (pH 9.1), boil for 3 minutes, and then add ice. Chilled. Add 20 ml of p-D ABA (p-dimethylaminobenzaldehyde) solution, react at 37 ° C for 20 minutes, transfer from each tube to a 96-well microphone mouthplate, and use a microplate reader. The absorbance at 585 nm was measured. For control, a buffer solution without sample was used. When the activity of hyaluronidase is inhibited, the degradation product N-acetylyldarcosamine (GlcNAc) decreases and the absorbance by p-DABA decreases.

[0067] Hyaluronidase inhibitory action is defined by the following equation.

Inhibition rate (%)

= (Control absorbance sample absorbance) / control absorbance X 100 Table 7 shows the results.

[0068] [Table 7]

[0069] As is apparent from Table 7, when the sample was added, an excellent inhibitory effect on hyaluronidase activity was observed. [0070] <Evaluation of antioxidant effect by scavenging DPPH radicals>

Using the extract obtained by the above extraction method 1 or extraction method 2 as a sample, the antioxidant effect by scavenging DPPH radical was evaluated as follows.

[0071] Using 50% by mass ethanol, the sample solution was adjusted so as to have the concentration of each sample shown in Table 8, and 100 aliquots were added to a 96-well microplate. Thereto, 0.2 mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added one by one 100 ml, mixed well, and allowed to stand at room temperature for 24 hours. Finally, the absorbance at 516 nm derived from DPPH radical was measured.

[0072] The extinction rate of DPPH radical was calculated from the following equation, where (A) was the force when the sample was added, and (A) the absorbance when the sample was added, and (B) the absorbance when the sample was added.

Radical scavenging rate = {1 (B) / (A)} X 100

The results are shown in Table 8.

[0073] [Table 8]

[0074] As is apparent from Table 8, when the sample was added, an excellent DPPH radical scavenging effect was observed.

[0075] <SOD-like activity evaluation (Evaluation of superoxide anion scavenging ability)>

Using the hot water extract obtained by the above extraction method 2 as a sample, SOD-like activity was evaluated as follows.

[0076] 0.25 mM WST— 1 and ImM Hypoxanthine-containing HANK 'S (+) solution 75 Was added. Furthermore, xanthine oxidase 25 ~ 1 (0.007 Units) was added and reacted at 37 ° C for 15 minutes, and then the absorbance at 450 nm was measured.

[0077] When the absorbance when only the HANK'S (+) solution is added instead of the sample solution is (A) and the absorbance when the sample solution is added is (B), the superoxide anion The erasure rate is defined as

Erasure rate (%) = {1— (B) / (A)} X100

The results obtained are shown in Table 9.

[Table 9]

[0079] As is apparent from Table 9, when the sample was added, an excellent superoxide anion erasing effect was observed.

[0080] Next, formulation examples for carrying out the present invention will be shown.

[0081] [Prescription Example 1] Emulsion

[Table A]

(1) Scorran 10. 0 (mass%)

(2) Methylphenylpolysiloxane 4.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Polyoxyethylene monostearate

Sorbitan (20 E. O.) 1. 3

(6) Sorbitan monostearate1.0

(7) Glycerin 4.0

(8) Methyl paraoxybenzoate 0.1

(9) Carboxyvinyl polymer 0.15

(10) Purified water 53. 85

(11) Arginine (1 mass% aqueous solution) 20. 0

(12) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Production method: Heat-dissolve the oil phase components of (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix evenly.

[Prescription Example 2] Lotion

[Table B] (1) Ethanol 15.0 (mass%)

(2) Polyoxyethylene (40E. O.) Hardened castor oil 0.3

(3) Fragrance 0. 1

(4) Purified water 78. 38

(5) Chenic acid 0.02

(6) Sodium quenate 0.1

(7) Glycerin 1.0

(8) Hydrochishetyl cellulose 0.1

(9) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Production method: Dissolve ( ² ) and (3) in (1). After dissolution, add (4) to (8) sequentially, and then stir well. Add (9) and mix uniformly.

[Prescription Example 3] Cream

[Table C]

(1) School run 10.0

(2) Stearic acid 2.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Setanol 3.6

(6) Lipophilic glyceryl monostearate2.0

(7) Glycerin 10.0

(8) Methyl paraoxybenzoate 0.1

(9) Arginine (20 mass% aqueous solution) 15. 0

(10) Purified water 36.7

(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15. 0

(12) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Production method: Heat-dissolve the oil phase components of (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, add (11), start cooling, add (12) at 40 ° C and mix uniformly.

[Prescription Example 4] Essence

[Table D] (1) Purified water 27. 45 (mass%)

(2) Glycerin 10.0

(3) Sucrose fatty acid ester 1.3

(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5

(5) Sodium alginate (1% by weight aqueous solution) 5.0

(6) Polyglyceryl monolaurate 1.0

(7) Macadamia nut oil fatty acid phytosteryl 3.0

(8) N-lauroyl-L-glutamic acid

Di (one phytosteryl 2-octyldodecyl) 2.0

(9) Hardened palm oil 2.0

(10) Sukuran (Olive) 1.0

(11) Behenino Reno Conoret 0. 75

(12) Beeslow 1. 0

(13) Jojoba oil1.0

(14) 1,3-Butylene glycol 10.0

(15) L-arginine (10 mass% aqueous solution) 2.0

(16) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Manufacturing method: Mix the water phase components of (1) to ½) and dissolve at 75 ° C with heating. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Start cooling after emulsification and add (15) at 50 ° C. Cool to 40 ° C, add (16), and mix evenly.

[Prescription Example 5] Aqueous jewel

[Table E]

(1) Forced L-Poxyvinyl Polymer 0.5

(2) Purified water 78.7

(3) Sodium hydroxide (10 mass. / ₀ aqueous solution) 0.5

(4) Ethanol 10.0

(5) Methyl paraoxybenzoate 0.1

(6) Fragrance 0. 1

(7) Cobannoashi extract (leaves) (Extraction method 1)

(8) Polyoxyethylene (60E. O.) Hardened castor oil 0.1 Manufacturing method: Add (1) to (2), stir uniformly, and then add (3). After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, add (6) to (8) previously mixed and stir and mix uniformly.

[Prescription Example 6] Cleansing Fee

[Table F] (1) Sukuran 77.0 (mass%)

(2) Polyoxyethylene glyceryl isostearate 15.0

(3) Purified water 3.0

(4) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Manufacturing method: Dissolve (1) and (2) uniformly. To this, add (3) and (4) in order and mix evenly.

[Table G]

(1) Stearic acid 16.0

(2) Myristic acid 16.0

(3) Lipophilic glyceryl monostearate2.0

(4) Glycerin 20.0

(5) Sodium hydroxide 7.5

(6) Coconut oil fatty acid amidopropyl betaine 1.0

(7) Purified water 31.5

(8) Copano reed extract (Leaf) (Extraction method 1) 6.0 Production method: Heat-dissolve the oil phase components of (1) to (4) at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C, and mixed with the oil phase components uniformly. Start cooling, add (8) at 40 ° C, and mix uniformly.

[Prescription Example 8] Makeup Base Cream

[Table H]

(1) Sukuran 10. 2 (;

(2) Cetanol 2.0

(3) Glycerol tri-2-ethylhexanoate 2.5

(4) Lipophilic glyceryl monostearate1.0

(5) Propylene Dariko Nore 1 1. 0

(6) Sucrose fatty acid ester 1.3

(7) Purified water 65.4

(8) Titanium oxide 1.0

(9) Bengala 0.1

(10) Yellow iron oxide 0.4

(11) Fragrance 0. 1

(12) Cobannoashi extract (leaves) (Extraction method 1) 5.0 Manufacturing method: Mix the oil phase components of (1) to (4) and dissolve at 75 ° C with heating. On the other hand, the aqueous phase components (5) to (7) are mixed, dissolved by heating at 75 ° C, and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C, and evenly To mix.

[Prescription Example 9] Milky Foundation

[Table I]

(1) Methyl polysiloxane 2.0 (mass ^_0/0)

(2) Skullang 5.0

(3) Octyldodecyl myristate 5.0

(4) Cetanol 1. 0

(5) Polyoxyethylene (20 E. O.)

Sonorebitan monostearate Estenole 1.3

(6) Sorbitan monostearate 0.7

(7) 1, 3-Butylene glycol 8.0

(8) Xanthan gum 0.1

(9) Methyl paraoxybenzoate 0.1

(10) Purified water 53.4

(11) Titanium oxide 9.0

(12) Talc 7.4

(13) Bengala 0.5

(14) Yellow iron oxide 1.1

(15) Black iron oxide 0.1

(16) Fragrance 0. 1

(17) Cobanno reed extract (Leaf) (Extraction method 1) 5.0 Manufacturing method: Mix the oil phase components of (1) to (6) and dissolve at 75 ° C with heating. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C, and the pigments (11) to (15) are added to this and dispersed uniformly with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after emulsification, and components (16) and (17) are added sequentially at 40 ° C and mixed uniformly.

[Prescription Example 10] Water-in-oil emollient cream

[Table J]

(1) Liquid paraffin 30. 0 (:

(2) Microcrystalline wax 2.0

(3) Vaseline 5.0

(4) Diglycerinoleate 5.0

(5) Sodium chloride 1.3

(6) Potassium chloride 0.1

(7) Propylene glycol 3.0

(8) 1, 3-butylene glycol 5.0

(9) Methyl paraoxybenzoate 0.1

(10) Cobannoashi extract (leaves) (Extraction method 1) 5.0

(11) Purified water 43.4

(12) Fragrance 0.1 Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C and heat to 50 ° C. Do not stir to (4) Gradually add. After mixing this, disperse uniformly into (1) to (3), which is heated and dissolved at 70 ° C. Add (7) to (10), which is dissolved in the remainder of (11) by heating at 70 ° C while stirring, and emulsify with a homomixer. Start cooling after emulsification, add (12) at 40 ° C, and mix evenly.

[Prescription Example 11] Pack

[Table K]

(1) Purified water 58.9 (mass ⁰ )

(2) Polyvinyl alcohol

(3) Ethanol 17.0

(4) Glycerin 5.0

(5) Polyethylene glycol (average molecular weight 1000) 2.0

(6) Cobannoashi extract (leaves) (Extraction method 1) 5.0

(7) Fragrance 0.1 Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and start cooling with stirring. Cool to 40 ° C, add (6) and (7), and mix evenly.

[Prescription Example 12] Bath salt

[Representation]

(1) Perfume 0.3 (mass ^_0/0)

(2) Copano reed extract (leaves) (Extraction method 1) 5.0

(3) Sodium bicarbonate 46.0

(4) Sodium sulfate 48.7 Production method: Mix (1) to (4) uniformly.

[Prescription Example 13] Hair wax

[Table M] (1) Stearic acid 3.0 (mass ° / ₀ )

(2) Microcrystalline wax 2.0

(3) Cetyl alcohol 3.0

(4) Highly polymerized methylpolysiloxane 2.0

(5) Methylpolysiloxane 5.0

(6) Poly (oxyethylene 'oxypropylene)

Methylpolysiloxane copolymer 1.0

(7) Methyl paraoxybenzoate 0.1

(8) 1,3-Butylene glycol 7.5

(9) Arginine 0.7

(10) Purified water 70.6

(11) Cobannoashi extract (leaves) (Extraction method 2) 5.0

(12) Fragrance 0.1 Production method: Mix the oil phase components (1) to (6), and heat and dissolve at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C, and the oil phase component is added and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly

[Prescription Example 14] Hair Nick

[Table N]

(1) Ethanol 46.0 (wt ^_0/0)

(2) Purified water 48.9

(3) Cobannoashi extract (leaves) (Extraction method 1) 5.0

(4) Perfume 0.1 Preparation: (1) _c of mixing, homogenizing the components to (4)

[0095] [Prescription Example 15] Beverage

[Table 0]

(1) Cobanno reed extract (Leaf: (Extraction method 1) 8.0 (Quality

(2) Erythritol 1.0

(3) Chenic acid 0.

(4) Stevia 0. 01

(5) Purified water 90. 89 Production method: Mix (1) to (5) uniformly.

[0096] [Prescription Example 16] Tablet

[Table P] (1) Cobanno reed extract (leaf) (Extraction method 2) 0.30 (part by mass)

(2) Reduced maltose starch syrup 0.5 3

(3) Corn starch 0.15

(4) Glycerin fatty acid ester 0.02 Production method: (1) to (3) were sieved and mixed, and (4) was further added and mixed. Tableting was performed with a tableting machine to obtain tablets with a total amount of 300 mg.

[0097] Next, a use test was conducted using a formulation in which an extract of a genus Sagaribana or a genus plant was mixed, and the improvement effect on rough skin due to drying was evaluated. At that time, in the formulation of the emulsion shown in Treatment Example 1, each of the extracts shown in Table 10 was blended as the component (12), and a use test was conducted as Examples;! As Comparative Example 1, an emulsion in which the extract of component (12) was replaced with purified water was used, and a use test was simultaneously conducted.

[0098] [Table 10]

[0099] 20 samples of dry skin women in their 30s to 50s who have markedly rough skin symptoms in each sample. Each group is a group of 20 skinners that were used blindly for 1 week. The change in state was observed and evaluated. As an index of skin symptoms, rough skin due to dryness was evaluated in three stages: “Improved”, “Slightly improved”, and “No change”.

Table 11 shows the number of panelists that obtained each evaluation.

[0100] [Table 11]

[0101] From Table 11, a clear improvement in skin roughness was observed in the example use group in which the sample was blended. From this, it has been clarified that the extract of the plant of the genus Sagaribana or the genus Botanus has an excellent moisturizing effect.

[0102] According to the present invention, a moisturizer, an anti-aging agent, a whitening agent, an anti-flame having an excellent effect by using a plant belonging to the genus Sagaribana or Botanus, or an extract thereof as an active ingredient. And anti-oxidants can be provided.

Furthermore, by combining the plant of the genus Sagaribana or the genus Botanus, or an extract thereof, with an external composition for skin (or a composition for external use) such as cosmetics or topical medicine, or an oral composition such as a food, It is possible to provide various compositions that exhibit excellent effects in preventing and improving various skin symptoms such as skin firmness or skin elasticity reduction, spots, dullness, dryness, and fine wrinkles.

The disclosure of the present application is related to the subject matter described in Japanese Patent Application No. 2006-253556 filed on Sep. 19, 2006, the entire disclosure of which is incorporated herein by reference. It should be noted that various modifications and changes may be made to the above-described embodiments without departing from the novel and advantageous features of the present invention other than those already described. Accordingly, all such modifications and changes are intended to be included within the scope of the appended claims.

Claims

The scope of the claims

[1] A moisturizer comprising one or more plants selected from the genus Sagaribana or the plant of the genus Botanus or an extract thereof as an active ingredient.

[2] An anti-aging agent comprising, as an active ingredient, one or more plants selected from the genus Sagaribana or the plant of the genus Botanus, or an extract thereof.

[3] A whitening agent comprising as an active ingredient one or more plants selected from the genus Sagaribana or the plant of the genus Botanus, or an extract thereof.

[4] An anti-inflammatory agent comprising, as an active ingredient, one or more plants selected from the genus Sagaribana or the plant of the genus Botanus, or an extract thereof.

[5] An antioxidant comprising as an active ingredient one or more plants selected from the genus Sagaribana or the plant of the genus Botanus, or an extract thereof.

[6] A composition for external use comprising one or more plants selected from the genus Sagaribana or the plant of the genus Botanus or an extract thereof.

[7] An oral composition containing one or more plants selected from the genus Sagaribana or the plant of the genus Botanus or an extract thereof.