WO2008009365A3 - Procédé servant à déterminer le taux de méthylation d'un acide nucléique - Google Patents
Procédé servant à déterminer le taux de méthylation d'un acide nucléique Download PDFInfo
- Publication number
- WO2008009365A3 WO2008009365A3 PCT/EP2007/006064 EP2007006064W WO2008009365A3 WO 2008009365 A3 WO2008009365 A3 WO 2008009365A3 EP 2007006064 W EP2007006064 W EP 2007006064W WO 2008009365 A3 WO2008009365 A3 WO 2008009365A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- base
- signal
- sequencing
- cytosine bases
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 10
- 102000039446 nucleic acids Human genes 0.000 title abstract 10
- 108020004707 nucleic acids Proteins 0.000 title abstract 10
- 238000000034 method Methods 0.000 title abstract 3
- 230000011987 methylation Effects 0.000 title abstract 2
- 238000007069 methylation reaction Methods 0.000 title abstract 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 8
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 abstract 4
- 238000012163 sequencing technique Methods 0.000 abstract 3
- 229940104302 cytosine Drugs 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000010606 normalization Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé servant à déterminer quantitativement le taux de méthylation d'un acide nucléique grâce au séquençage. Selon l'invention, le procédé comprend au moins les étapes suivantes consistant à : a) traiter l'acide nucléique avec un réactif chimique ou une solution contenant une enzyme, ce par quoi le comportement d'appariement des bases pour les bases de type cytosines méthylées et/ou les bases de type cytosines non méthylées de l'acide nucléique est modifié de façon à ce que les bases de type cytosines méthylées deviennent distinguables des bases de type cytosines non méthylées ; b) introduire dans l'acide nucléique au moins une base servant à générer un signal de séquençage à utiliser en tant que signal de référence pour la normalisation ; c) séquencer l'acide nucléique, ce par quoi on obtient un signal provenant de chaque base de type cytosine de l'acide nucléique ou un signal provenant de chaque base de type guanine de l'acide nucléique et un signal de référence provenant de ladite ou desdites bases introduites ; et d) normaliser le signal obtenu à partir de chaque base de type cytosine de l'acide nucléique ou le signal obtenu à partir de chaque base de type guanine de l'acide nucléique par rapport au signal de référence provenant de ladite ou desdites bases introduites.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/309,555 US20100003680A1 (en) | 2006-07-18 | 2006-07-18 | Method For Determining The Methylation Rate of a Nucleic Acid |
| EP07785943A EP2044214A2 (fr) | 2006-07-18 | 2007-07-09 | Procédé servant à déterminer le taux de méthylation d'un acide nucléique |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06090125.3 | 2006-07-18 | ||
| EP06090125 | 2006-07-18 | ||
| EP06120694.2 | 2006-09-14 | ||
| EP06120694 | 2006-09-14 | ||
| EP07109855.2 | 2007-06-08 | ||
| EP07109855 | 2007-06-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2008009365A2 WO2008009365A2 (fr) | 2008-01-24 |
| WO2008009365A3 true WO2008009365A3 (fr) | 2008-04-17 |
Family
ID=38935863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2007/006064 WO2008009365A2 (fr) | 2006-07-18 | 2007-07-09 | Procédé servant à déterminer le taux de méthylation d'un acide nucléique |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP2044214A2 (fr) |
| WO (1) | WO2008009365A2 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2689537C (fr) * | 2007-06-07 | 2016-08-23 | Simons Haplomics Limited | Methodes epigenetiques |
| JP5757572B2 (ja) * | 2009-07-03 | 2015-07-29 | 国立大学法人 東京大学 | 癌細胞の存否を判定する方法および癌患者の予後を判定する方法 |
| US10570455B2 (en) | 2013-05-10 | 2020-02-25 | University Of Southern California | DNA methylation biomarkers for bladder cancer |
| US20200131566A1 (en) * | 2018-10-31 | 2020-04-30 | Guardant Health, Inc. | Methods, compositions and systems for calibrating epigenetic partitioning assays |
-
2007
- 2007-07-09 EP EP07785943A patent/EP2044214A2/fr not_active Withdrawn
- 2007-07-09 WO PCT/EP2007/006064 patent/WO2008009365A2/fr active Application Filing
Non-Patent Citations (5)
| Title |
|---|
| HAN ET AL: "DNA methylation mapping by tag-modified bisulfite genomic sequencing", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 355, no. 1, 2 June 2006 (2006-06-02), pages 50 - 61, XP005542202, ISSN: 0003-2697 * |
| JANG SE JIN ET AL: "Methylation patterns in human androgen receptor gene and clonality analysis", CANCER RESEARCH, vol. 60, no. 4, 15 February 2000 (2000-02-15), pages 864 - 866, XP002467560, ISSN: 0008-5472 * |
| LEWIN JOERN ET AL: "Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates", BIOINFORMATICS (OXFORD), vol. 20, no. 17, 22 November 2004 (2004-11-22), pages 3005 - 3012, XP002467526, ISSN: 1367-4803 * |
| PAUL C L ET AL: "Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN analysis", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, vol. 21, no. 1, July 1996 (1996-07-01), pages 126 - 133, XP002143107, ISSN: 0736-6205 * |
| YANG INCHUL ET AL: "Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR.", NUCLEIC ACIDS RESEARCH 2006, vol. 34, no. 8, 2006, pages e61, XP002467528, ISSN: 1362-4962 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008009365A2 (fr) | 2008-01-24 |
| EP2044214A2 (fr) | 2009-04-08 |
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