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WO2008005998A3 - Incubateur de diagnostic intégré à l'échelle du centimètre pour cultures biologiques - Google Patents

Incubateur de diagnostic intégré à l'échelle du centimètre pour cultures biologiques Download PDF

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Publication number
WO2008005998A3
WO2008005998A3 PCT/US2007/072777 US2007072777W WO2008005998A3 WO 2008005998 A3 WO2008005998 A3 WO 2008005998A3 US 2007072777 W US2007072777 W US 2007072777W WO 2008005998 A3 WO2008005998 A3 WO 2008005998A3
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
incubators
enclosure
disposed
culturing
Prior art date
Application number
PCT/US2007/072777
Other languages
English (en)
Other versions
WO2008005998A2 (fr
Inventor
Jelena Vukasinovic
Ari Glezer
Original Assignee
Georgia Tech Res Inst
Jelena Vukasinovic
Ari Glezer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/483,126 external-priority patent/US7855070B2/en
Application filed by Georgia Tech Res Inst, Jelena Vukasinovic, Ari Glezer filed Critical Georgia Tech Res Inst
Priority to US12/529,881 priority Critical patent/US20100151571A1/en
Publication of WO2008005998A2 publication Critical patent/WO2008005998A2/fr
Publication of WO2008005998A3 publication Critical patent/WO2008005998A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne des incubateurs de diagnostic intégrés, portables, jetables, à l'échelle du centimètre, utilisables pour les cultures biologiques. Un incubateur à titre d'exemple comprend une enceinte optiquement accessible ayant une pluralité de ports pour fluides. Un élément chauffant installé à l'intérieur de l'enceinte est raccordé à une commande de chauffage externe. Une chambre de perfusion de micro-fluides pouvant passer à l'autoclave est installée à l'intérieur de l'enceinte qui comprend une chambre d'entretien de la vie de culture de cellules, un port d'entrée placé dans la chambre de perfusion, une chambre de prélèvement en communication avec la chambre de culture, un port de sortie raccordé à la réserve de prélèvements et un substrat de perfusion. Une membrane optiquement transparente et perméable aux gaz peut être fixée sur le haut de la chambre de perfusion. Les incubateurs sont accessibles optiquement et ils sont équipés d'une commande de l'écoulement des fluides et d'une régulation de la température, ils sont portables et modulaires et leur construction est bon marché. Les incubateurs permettent de pratiquer des essais de médicaments et des cultures de tissus biologiques sur place.
PCT/US2007/072777 2006-07-07 2007-07-03 Incubateur de diagnostic intégré à l'échelle du centimètre pour cultures biologiques WO2008005998A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/529,881 US20100151571A1 (en) 2006-07-07 2007-07-03 Centimeter-scale, integrated diagnostics incubator for biological culturing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/483,126 US7855070B2 (en) 2005-07-08 2006-07-07 Centimeter-scale, integrated diagnostics incubator for biological culturing
US11/483,126 2006-07-07
US85122206P 2006-10-12 2006-10-12
US60/851,222 2006-10-12

Publications (2)

Publication Number Publication Date
WO2008005998A2 WO2008005998A2 (fr) 2008-01-10
WO2008005998A3 true WO2008005998A3 (fr) 2008-10-09

Family

ID=38895452

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/072777 WO2008005998A2 (fr) 2006-07-07 2007-07-03 Incubateur de diagnostic intégré à l'échelle du centimètre pour cultures biologiques

Country Status (1)

Country Link
WO (1) WO2008005998A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2329986T3 (es) 2001-09-06 2009-12-03 Rapid Micro Biosystems Inc Deteccion rapida de celulas en replicacion.
EP1937829A4 (fr) 2005-09-26 2011-08-03 Rapid Micro Biosystems Inc Cassette contenant un milieu de croissance
US20110269226A1 (en) * 2008-08-27 2011-11-03 Agency For Science, Technology And Research Microfluidic Continuous Flow Device for Culturing Biological Material
US10384203B2 (en) 2008-09-24 2019-08-20 First Light Biosciences, Inc. Kits and devices for detecting analytes
DE102008056037B4 (de) * 2008-11-05 2012-10-31 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mikrofluidischer Bioreaktor
GB0820779D0 (en) * 2008-11-13 2008-12-17 Artelis S A Cell culture device and method of culturing cells
EP2613882B1 (fr) * 2010-09-10 2016-03-02 Gradientech AB Capsule microfluidique
EP3327110B1 (fr) 2011-11-07 2020-01-29 Rapid Micro Biosystems, Inc. Cassette pour test de stérilité
CN107841461A (zh) * 2012-04-16 2018-03-27 快速微型生物系统公司 细胞培养装置
WO2019204784A1 (fr) 2018-04-19 2019-10-24 First Light Biosciences, Inc. Détection d'anticorps
WO2020073019A2 (fr) 2018-10-04 2020-04-09 First Light Diagnostics, Inc. Détection et analyse de cellules
CN110108622B (zh) * 2019-06-17 2024-02-02 辽宁中医药大学 一种离体微血管内皮屏障功能检测方法与设备
CN111205980B (zh) * 2020-01-20 2024-01-26 河海大学常州校区 一种单细胞原位实时显微观测的细胞培养装置及其操作方法

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879876A (en) * 1997-03-24 1999-03-09 Lifenet Continuous-multi-step dilution process and apparatus, for the removal of cryoprotectants from cryopreserved tissues
US6057150A (en) * 1997-09-19 2000-05-02 Bio-Rad Laboratories, Inc. Biaxial strain system for cultured cells
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US6368838B1 (en) * 1993-10-04 2002-04-09 President And Fellows Of Havard College Adhering cells to cytophilic islands separated by cytophobic regions to form patterns and manipulate cells
US20030004394A1 (en) * 2001-03-13 2003-01-02 Carpay Wilhelmus Marinus Incubator system provided with a temperature control system
US6503751B2 (en) * 1996-02-09 2003-01-07 Thermo Forma Inc. Controlled atmosphere incubator
US20040132175A1 (en) * 2000-07-19 2004-07-08 Jerome Vetillard Cell culture chamber and bioreactor for extracorporeal culture of animal cells
US20050089993A1 (en) * 2002-05-01 2005-04-28 Paolo Boccazzi Apparatus and methods for simultaneous operation of miniaturized reactors
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050186671A1 (en) * 2000-10-02 2005-08-25 Cannon Thomas F. Automated bioculture and bioculture experiments system
US20050221269A1 (en) * 2004-04-05 2005-10-06 Organ Recovery Systems Apparatus and method for perfusing an organ or tissue for isolating cells from the organ or tissue
US20060073539A1 (en) * 2001-08-06 2006-04-06 Wikswo John P Apparatus and methods for using biological material to discriminate an agent

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6368838B1 (en) * 1993-10-04 2002-04-09 President And Fellows Of Havard College Adhering cells to cytophilic islands separated by cytophobic regions to form patterns and manipulate cells
US6503751B2 (en) * 1996-02-09 2003-01-07 Thermo Forma Inc. Controlled atmosphere incubator
US5879876A (en) * 1997-03-24 1999-03-09 Lifenet Continuous-multi-step dilution process and apparatus, for the removal of cryoprotectants from cryopreserved tissues
US6057150A (en) * 1997-09-19 2000-05-02 Bio-Rad Laboratories, Inc. Biaxial strain system for cultured cells
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US20040132175A1 (en) * 2000-07-19 2004-07-08 Jerome Vetillard Cell culture chamber and bioreactor for extracorporeal culture of animal cells
US20050186671A1 (en) * 2000-10-02 2005-08-25 Cannon Thomas F. Automated bioculture and bioculture experiments system
US20030004394A1 (en) * 2001-03-13 2003-01-02 Carpay Wilhelmus Marinus Incubator system provided with a temperature control system
US20060073539A1 (en) * 2001-08-06 2006-04-06 Wikswo John P Apparatus and methods for using biological material to discriminate an agent
US20050089993A1 (en) * 2002-05-01 2005-04-28 Paolo Boccazzi Apparatus and methods for simultaneous operation of miniaturized reactors
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050221269A1 (en) * 2004-04-05 2005-10-06 Organ Recovery Systems Apparatus and method for perfusing an organ or tissue for isolating cells from the organ or tissue

Also Published As

Publication number Publication date
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