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WO2008000277A3 - Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés - Google Patents

Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés Download PDF

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Publication number
WO2008000277A3
WO2008000277A3 PCT/DK2007/050079 DK2007050079W WO2008000277A3 WO 2008000277 A3 WO2008000277 A3 WO 2008000277A3 DK 2007050079 W DK2007050079 W DK 2007050079W WO 2008000277 A3 WO2008000277 A3 WO 2008000277A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
cytochrome
fungal cells
polyunsaturated fatty
increased content
Prior art date
Application number
PCT/DK2007/050079
Other languages
English (en)
Other versions
WO2008000277A2 (fr
WO2008000277A8 (fr
Inventor
Jochen Foerster
Nina Katarina Gunnarsson
Malin Inga Elisabeth Svensson
Helga Susana Moreira David
Iben Plate
Sabina De Andrade Pere Tavares
Jean-Marie Mouillion
Sara Petersen Bjoern
Per Moeller
Original Assignee
Fluxome Sciences As
Jochen Foerster
Nina Katarina Gunnarsson
Malin Inga Elisabeth Svensson
Helga Susana Moreira David
Iben Plate
Sabina De Andrade Pere Tavares
Jean-Marie Mouillion
Sara Petersen Bjoern
Per Moeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluxome Sciences As, Jochen Foerster, Nina Katarina Gunnarsson, Malin Inga Elisabeth Svensson, Helga Susana Moreira David, Iben Plate, Sabina De Andrade Pere Tavares, Jean-Marie Mouillion, Sara Petersen Bjoern, Per Moeller filed Critical Fluxome Sciences As
Publication of WO2008000277A2 publication Critical patent/WO2008000277A2/fr
Publication of WO2008000277A3 publication Critical patent/WO2008000277A3/fr
Publication of WO2008000277A8 publication Critical patent/WO2008000277A8/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/004—Cytochrome-b5 reductase (1.6.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne la production d'acides gras et, plus particulièrement, la production d'acide arachidonique (ARA) et d'acide eicosapentanoïque (EPA) d'acides gras polyinsaturés (PUFA) dans des cellules fongiques génétiquement modifiées, notamment, des cellules de Saccharomyces cerevisiae métaboliquement modifiées avec une teneur élevée en ARA et en EPA. L'invention se rapporte particulièrement à l'accroissement de la teneur en PUFA dans l'organisme hôte au moyen, par exemple, de diverses sur-expressions du cytochrome b5 et de la réductase du cytochrome b5 impliquées dans la désaturation d'acide gras, et de l'expression hétérologue du cytochrome b5 et de la réductase de cytochrome b5 ainsi que de l'expression de synthases d'acides gras hétérologues.
PCT/DK2007/050079 2006-06-29 2007-06-29 Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés WO2008000277A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81764306P 2006-06-29 2006-06-29
US60/817,643 2006-06-29

Publications (3)

Publication Number Publication Date
WO2008000277A2 WO2008000277A2 (fr) 2008-01-03
WO2008000277A3 true WO2008000277A3 (fr) 2008-03-20
WO2008000277A8 WO2008000277A8 (fr) 2009-07-30

Family

ID=37188928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2007/050079 WO2008000277A2 (fr) 2006-06-29 2007-06-29 Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés

Country Status (1)

Country Link
WO (1) WO2008000277A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0725075D0 (en) * 2007-12-21 2008-01-30 Fluxome Sciences As Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
EP2108702A1 (fr) * 2008-04-07 2009-10-14 Organo Balance GmbH Synthèse d'acides gras polyinsaturés dans la levure
FR2940315B1 (fr) * 2008-12-19 2012-12-14 Airbus France Nouveau procede de culture de levures du genre yarrowia
EP3075848A1 (fr) 2015-04-01 2016-10-05 Johann Wolfgang Goethe-Universität Frankfurt am Main Production microbiologique d'acides gras courts et leurs utilisations
KR101957910B1 (ko) 2017-05-02 2019-03-15 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 효모의 세포 소기관(organelle) 개량을 통한 진세노사이드 생산 증대
EP4004187A1 (fr) * 2019-07-26 2022-06-01 Novozymes A/S Cellule hôte fongique filamenteuse modifiée
MX2023013231A (es) * 2021-05-10 2024-01-24 Fmc Agricultural Solutions As Metodos y celulas mejorados para aumentar la actividad enzimatica y la produccion de feromonas de insectos.
CN114478727A (zh) * 2022-01-17 2022-05-13 华中科技大学 高山被孢霉油脂合成相关转录因子ino4及其编码基因与应用
CN118956990A (zh) * 2024-09-30 2024-11-15 四川轻化工大学 一种利用异常威克汉姆酵母生产低冷滤点生物柴油的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0978563A1 (fr) * 1997-02-27 2000-02-09 Suntory Limited Gene de delta 9-desaturase
WO2005118814A2 (fr) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Cellules metaboliquement transformees pour la production d'acides gras polyinsaturates

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0978563A1 (fr) * 1997-02-27 2000-02-09 Suntory Limited Gene de delta 9-desaturase
WO2005118814A2 (fr) * 2004-06-04 2005-12-15 Fluxome Sciences A/S Cellules metaboliquement transformees pour la production d'acides gras polyinsaturates

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BEAUDOIN FREDERIC ET AL: "Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 97, no. 12, 6 June 2000 (2000-06-06), pages 6421 - 6426, XP002200201, ISSN: 0027-8424 *
VEEN M ET AL: "Production of lipid compounds in the yeast Saccharomyces cerevisiae.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 63, no. 6, February 2004 (2004-02-01), pages 635 - 646, XP002405982, ISSN: 0175-7598 *

Also Published As

Publication number Publication date
WO2008000277A2 (fr) 2008-01-03
WO2008000277A8 (fr) 2009-07-30

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