+

WO2008053019A3 - Method for reducing the expression of a gene in a filamentous fungal cell - Google Patents

Method for reducing the expression of a gene in a filamentous fungal cell Download PDF

Info

Publication number
WO2008053019A3
WO2008053019A3 PCT/EP2007/061768 EP2007061768W WO2008053019A3 WO 2008053019 A3 WO2008053019 A3 WO 2008053019A3 EP 2007061768 W EP2007061768 W EP 2007061768W WO 2008053019 A3 WO2008053019 A3 WO 2008053019A3
Authority
WO
WIPO (PCT)
Prior art keywords
filamentous fungal
gene
expression
fungal cell
cell
Prior art date
Application number
PCT/EP2007/061768
Other languages
French (fr)
Other versions
WO2008053019A2 (en
Inventor
Van Noel Nicolaas Maria Eisabeth Peij
Lucie Parenicova
Mojca Bencina
Karolina Ivicak
Original Assignee
Dsm Ip Assets Bv
Peij Van Noel Nicolaas Maria Elisabeth
Lucie Parenicova
Mojca Bencina
Karolina Ivicak
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv, Peij Van Noel Nicolaas Maria Elisabeth, Lucie Parenicova, Mojca Bencina, Karolina Ivicak filed Critical Dsm Ip Assets Bv
Publication of WO2008053019A2 publication Critical patent/WO2008053019A2/en
Publication of WO2008053019A3 publication Critical patent/WO2008053019A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a method for reducing the expression of a gene in a filamentous fungal cell. The invention further relates to a modified filamentous fungal cell suitable for the production of a compound of interest, wherein, compared to the parental cell the modified cell derives from, said modified cell exhibits improved abilities for RNAi mediated reduction of gene expression of a target gene. In addition, the invention relates to a method for the production of a compound of interest in said modified filamentous fungal cell.
PCT/EP2007/061768 2006-11-02 2007-10-31 Method for reducing the expression of a gene in a filamentous fungal cell WO2008053019A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06123393.8 2006-11-02
EP06123393 2006-11-02

Publications (2)

Publication Number Publication Date
WO2008053019A2 WO2008053019A2 (en) 2008-05-08
WO2008053019A3 true WO2008053019A3 (en) 2009-01-22

Family

ID=39344639

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/061768 WO2008053019A2 (en) 2006-11-02 2007-10-31 Method for reducing the expression of a gene in a filamentous fungal cell

Country Status (1)

Country Link
WO (1) WO2008053019A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
WO2012001169A1 (en) 2010-07-01 2012-01-05 Dsm Ip Assets B.V. A method for the production of a compound of interest
WO2014013074A1 (en) 2012-07-19 2014-01-23 Dsm Ip Assets B.V. Agse-deficient strain
US9598698B2 (en) 2012-08-17 2017-03-21 Novozymes A/S Methods for co-silencing expression of genes in filamentous fungal strains and uses thereof
CN105452476B (en) 2013-05-31 2020-08-14 帝斯曼知识产权资产管理有限公司 Microorganism for diterpene production
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
US11396665B2 (en) 2015-01-06 2022-07-26 Dsm Ip Assets B.V. CRISPR-CAS system for a filamentous fungal host cell
US10590436B2 (en) 2015-01-06 2020-03-17 Dsm Ip Assets B.V. CRISPR-CAS system for a lipolytic yeast host cell
US10619170B2 (en) 2015-01-06 2020-04-14 Dsm Ip Assets B.V. CRISPR-CAS system for a yeast host cell
EP3516056B1 (en) 2016-09-23 2024-11-27 DSM IP Assets B.V. A guide-rna expression system for a host cell
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
EP3607071A1 (en) 2017-04-06 2020-02-12 DSM IP Assets B.V. Self-guiding integration construct (sgic)
WO2019012162A2 (en) 2017-12-20 2019-01-17 Dsm Ip Assets B.V. A method for genome editing in a host cell
WO2019063849A1 (en) 2017-12-20 2019-04-04 Dsm Ip Assets B.V. Purification of a polypeptide of interest
WO2019215102A1 (en) 2018-05-09 2019-11-14 Dsm Ip Assets B.V. Crispr transient expression construct (ctec)
EP3891281A1 (en) 2018-12-05 2021-10-13 DSM IP Assets B.V. Crispr guide-rna expression strategies for multiplex genome engineering
US20220235378A1 (en) 2019-05-06 2022-07-28 Dsm Ip Assets B.V. Multipartite crispr donor
JP7496411B2 (en) * 2019-08-09 2024-06-06 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド Anti-porcine TCN1 monoclonal antibodies and methods for making and using same
EP4055170A1 (en) 2019-11-04 2022-09-14 DSM IP Assets B.V. Low volume transfection
CA3198626A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2024218244A1 (en) 2023-04-20 2024-10-24 Dsm Ip Assets B.V. Strains and methods for the production of mogrosides

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056772A1 (en) * 2003-12-09 2005-06-23 Novozymes Inc. Methods for eliminating or reducing the expression of genes in filamentous fungal strains
EP1681347A1 (en) * 2005-01-18 2006-07-19 Metanomics GmbH & Co. KGaA Improved methods for double-stranded RNA mediated gene silencing
WO2007056826A1 (en) * 2005-11-21 2007-05-24 Johnson & Johnson Research Pty Limited Multitargeting interfering rnas and methods of their use and design

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056772A1 (en) * 2003-12-09 2005-06-23 Novozymes Inc. Methods for eliminating or reducing the expression of genes in filamentous fungal strains
EP1681347A1 (en) * 2005-01-18 2006-07-19 Metanomics GmbH & Co. KGaA Improved methods for double-stranded RNA mediated gene silencing
WO2007056826A1 (en) * 2005-11-21 2007-05-24 Johnson & Johnson Research Pty Limited Multitargeting interfering rnas and methods of their use and design

Non-Patent Citations (25)

* Cited by examiner, † Cited by third party
Title
ANDERSON J ET AL: "Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 confer HIV-1 resistance", ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, MARY ANN LIEBERT, INC., NEW YORK, US, vol. 13, no. 5, 1 January 2003 (2003-01-01), pages 303 - 312, XP002313468, ISSN: 1087-2906 *
CHOUDHARY SWATI ET AL: "A double-stranded-RNA response program important for RNA interference efficiency.", MOLECULAR AND CELLULAR BIOLOGY JUN 2007, vol. 27, no. 11, June 2007 (2007-06-01), pages 3995 - 4005, XP002488624, ISSN: 0270-7306 *
CHUNG KWAN-HO ET AL: "Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 34, no. 7, 13 April 2006 (2006-04-13), pages e53 - 1, XP002456694, ISSN: 0305-1048 *
DATABASE UniProt [online] 6 March 2007 (2007-03-06), "Contig An03c0070, complete genome.", XP002488632, retrieved from EBI accession no. UNIPROT:A2QG96 Database accession no. A2QG96 *
DE BACKER MARIANNE D ET AL: "RNA-mediated gene silencing in non-pathogenic and pathogenic fungi.", CURRENT OPINION IN MICROBIOLOGY JUN 2002, vol. 5, no. 3, June 2002 (2002-06-01), pages 323 - 329, XP002488628, ISSN: 1369-5274 *
DIETZL GEORG ET AL: "A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.", NATURE 12 JUL 2007, vol. 448, no. 7150, 12 July 2007 (2007-07-12), pages 151 - 156, XP002488881, ISSN: 1476-4687 *
DUXBURY MARK S ET AL: "RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 3 JUN 2005, vol. 331, no. 2, 3 June 2005 (2005-06-03), pages 459 - 463, XP004867577, ISSN: 0006-291X *
HAMMOND T M ET AL: "RNA silencing in Aspergillus nidulans is independent of RNA-dependent RNA polymerases.", GENETICS FEB 2005, vol. 169, no. 2, February 2005 (2005-02-01), pages 607 - 617, XP002488631, ISSN: 0016-6731 *
HENRY ET AL: "Simultaneous targeting of HCV replication and viral binding with a single lentiviral vector containing multiple RNA interference expression cassettes", MOLECULAR THERAPY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 14, no. 4, 1 October 2006 (2006-10-01), pages 485 - 493, XP005644832, ISSN: 1525-0016 *
JOYCE P.I. ET AL.: "Manipulating and enhancing the RNAi response", JOURNAL OF RNAI AND GENE SILENCING, vol. 2, no. 1, February 2006 (2006-02-01), pages 118 - 125, XP002488882 *
KADOTANI N ET AL: "One of the Two Dicer-like Proteins in the Filamentous Fungi Magnaporthe oryzae Genome Is Responsible for Hairpin RNA-triggered RNA Silencing and Related Small Interfering RNA Accumulation", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,; US, vol. 279, no. 43, 22 October 2004 (2004-10-22), pages 44467 - 44474, XP002986552, ISSN: 0021-9258 *
LEIRDAL M ET AL: "Gene silencing in mammalian cells by preformed small RNA duplexes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 295, 1 June 2002 (2002-06-01), pages 744 - 748, XP002953281, ISSN: 0006-291X *
LIU YING POI ET AL: "Design of extended short hairpin RNAs for HIV-1 inhibition.", NUCLEIC ACIDS RESEARCH 2007, vol. 35, no. 17, 21 August 2007 (2007-08-21), pages 5683 - 5693, XP002501890, ISSN: 1362-4962 *
LO H-L ET AL: "Inhibition of HIV-1 replication with designed miRNAs expressed from RNA polymerase II promoters.", GENE THERAPY NOV 2007, vol. 14, no. 21, 6 September 2007 (2007-09-06), pages 1503 - 1512, XP002501891, ISSN: 0969-7128 *
MIKUMA TOSHIYASU ET AL: "Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.", NUCLEIC ACIDS SYMPOSIUM SERIES (2004) 2004, no. 48, 2004, pages 191 - 192, XP002488623, ISSN: 1746-8272 *
NAKAYASHIKI HITOSHI ET AL: "Evolution and diversification of RNA silencing proteins in fungi.", JOURNAL OF MOLECULAR EVOLUTION JUL 2006, vol. 63, no. 1, July 2006 (2006-07-01), pages 127 - 135, XP002488629, ISSN: 0022-2844 *
NAKAYASHIKI HITOSHI: "RNA silencing in fungi: mechanisms and applications.", FEBS LETTERS 31 OCT 2005, vol. 579, no. 26, 31 October 2005 (2005-10-31), pages 5950 - 5957, XP002488630, ISSN: 0014-5793 *
PEL H. ET AL.: "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88., Supplementary Table 7", NATURE BIOTECHNOLOGY, February 2007 (2007-02-01), XP002488625, Retrieved from the Internet <URL:http://www.nature.com/nbt/journal/v25/n2/extref/nbt1282-S14.pdf> [retrieved on 20080716] *
PEL HERMAN J ET AL: "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88.", NATURE BIOTECHNOLOGY FEB 2007, vol. 25, no. 2, February 2007 (2007-02-01), pages 221 - 231, XP002458027, ISSN: 1087-0156 *
RAPONI MITCH ET AL: "Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast.", NUCLEIC ACIDS RESEARCH 1 JUN 2002, vol. 30, no. 11, 1 June 2002 (2002-06-01), pages 2546 - 2554, XP002488627, ISSN: 1362-4962 *
RAPONI MITCH ET AL: "Double-stranded RNA-mediated gene silencing in fission yeast.", NUCLEIC ACIDS RESEARCH 1 AUG 2003, vol. 31, no. 15, 1 August 2003 (2003-08-01), pages 4481 - 4489, XP002488626, ISSN: 1362-4962 *
SCHERR MICHAELA ET AL: "Gene silencing by small regulatory RNAs in mammalian cells.", CELL CYCLE (GEORGETOWN, TEX.) 15 FEB 2007, vol. 6, no. 4, 15 February 2007 (2007-02-15), pages 444 - 449, XP002502224, ISSN: 1551-4005 *
WANG SHUNQING ET AL: "Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing.", BMC BIOTECHNOLOGY 2006, vol. 6, 2006, pages 50, XP002501889, ISSN: 1472-6750 *
YI RUI ET AL: "Overexpression of Exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs", RNA (COLD SPRING HARBOR), vol. 11, no. 2, February 2005 (2005-02-01), pages 220 - 226, XP002488883, ISSN: 1355-8382 *
ZHU XIAOCUI ET AL: "A versatile approach to multiple gene RNA interference using microRNA-based short hairpin RNAs", BMC MOLECULAR BIOLOGY, BIOMED CENTRAL LTD, GB, vol. 8, no. 1, 30 October 2007 (2007-10-30), pages 98, XP021033443, ISSN: 1471-2199 *

Also Published As

Publication number Publication date
WO2008053019A2 (en) 2008-05-08

Similar Documents

Publication Publication Date Title
WO2008053019A3 (en) Method for reducing the expression of a gene in a filamentous fungal cell
EP2478769B8 (en) Process for producing flours from fungus myceliated grain
WO2011061032A3 (en) Cells, nucleic acids, enzymes, and use thereof, and methods for the production of sophorolipids
WO2009076400A3 (en) Compositions and methods for inhibiting expression of factor vii gene
WO2009141837A3 (en) Process for preparing posaconazole and intermediates thereof
WO2008153712A3 (en) Method for introducing nucleic acids into fungal cells
WO2007106738A3 (en) Acrylated hyaluronic acid
WO2011150241A8 (en) Decreasing lactate level and increasing polypeptide production by downregulating ldh and pdhk expression
WO2009113853A3 (en) Adipate ester or thioester synthesis
WO2009123762A3 (en) Method of screening single cells for the production of biologically active agents
WO2010042664A3 (en) Method and compositions for producing fatty aldehydes
WO2009150232A3 (en) Manufacture process for the preparation of an iron containing phosphate adsorbent
WO2012076124A3 (en) Antibacterial coating for an implant and method for producing said coating
WO2013000717A3 (en) Dermatologically effective yeast extract
WO2008066558A3 (en) Process for the preparation of 2-substituted-5-(1-alkylthio)alkylpyridines
WO2009081012A3 (en) Method for producing succinic acid
EP2299452A4 (en) Microstructure, and method for production thereof
WO2007014197A3 (en) Yeast expression vectors for production of itf
WO2011004402A3 (en) Improved process for the preparation of ambrisentan and novel intermediates thereof
WO2014057419A3 (en) Aldehyde acetal based processes for the manufacture of macrocyclic depsipeptides and new intermediates
WO2013040062A3 (en) Characterizing multiple sclerosis
WO2007132354A3 (en) Process for preparing voriconazole, new polymorphic form of intermediate thereof, and uses thereof
WO2008075205A3 (en) Improved process for the preparation of voriconazole
WO2011000361A3 (en) Method for the production and series connection of strip-shaped elements on a substrate
WO2011151512A3 (en) Method for protein production in filamentous fungi

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07822115

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07822115

Country of ref document: EP

Kind code of ref document: A2

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载