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WO2008046922A3 - Méthode d'analyse des variations de séquence dans la zone génomique ns5b du vhc - Google Patents

Méthode d'analyse des variations de séquence dans la zone génomique ns5b du vhc Download PDF

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Publication number
WO2008046922A3
WO2008046922A3 PCT/EP2007/061241 EP2007061241W WO2008046922A3 WO 2008046922 A3 WO2008046922 A3 WO 2008046922A3 EP 2007061241 W EP2007061241 W EP 2007061241W WO 2008046922 A3 WO2008046922 A3 WO 2008046922A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence variations
anaysis
methodology
hcv ns5b
genomic region
Prior art date
Application number
PCT/EP2007/061241
Other languages
English (en)
Other versions
WO2008046922A2 (fr
Inventor
Erwin Sablon
Evelien Libbrecht
Original Assignee
Innogenetics Nv
Erwin Sablon
Evelien Libbrecht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innogenetics Nv, Erwin Sablon, Evelien Libbrecht filed Critical Innogenetics Nv
Priority to US12/311,932 priority Critical patent/US20090325145A1/en
Priority to EP07821605A priority patent/EP2121990A2/fr
Publication of WO2008046922A2 publication Critical patent/WO2008046922A2/fr
Publication of WO2008046922A3 publication Critical patent/WO2008046922A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé normalisé d'amplification d'un fragment d'acide nucléique NS5B du VHC de l'un quelconque des génotypes 1 à 6, en tant qu'outil pour l'analyse des variations de séquence qui peuvent être mises en corrélation avec la résistance aux médicaments pour lutter contre le VHC.
PCT/EP2007/061241 2006-10-20 2007-10-19 Méthode d'analyse des variations de séquence dans la zone génomique ns5b du vhc WO2008046922A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/311,932 US20090325145A1 (en) 2006-10-20 2007-10-19 Methodology for analysis of sequence variations within the hcv ns5b genomic region
EP07821605A EP2121990A2 (fr) 2006-10-20 2007-10-19 Méthode d'analyse des variations de séquence dans la zone génomique ns5b du vhc

Applications Claiming Priority (20)

Application Number Priority Date Filing Date Title
US85301506P 2006-10-20 2006-10-20
US85298406P 2006-10-20 2006-10-20
US85301606P 2006-10-20 2006-10-20
US85298206P 2006-10-20 2006-10-20
US85298306P 2006-10-20 2006-10-20
EP06122673 2006-10-20
US60/853,015 2006-10-20
EP06122678.3 2006-10-20
EP06122668 2006-10-20
EP06122675.9 2006-10-20
US60/852,982 2006-10-20
US60/853,016 2006-10-20
US60/852,983 2006-10-20
EP06122668.4 2006-10-20
EP06122678 2006-10-20
EP06122670 2006-10-20
EP06122670.0 2006-10-20
US60/852,984 2006-10-20
EP06122673.4 2006-10-20
EP06122675 2006-10-20

Publications (2)

Publication Number Publication Date
WO2008046922A2 WO2008046922A2 (fr) 2008-04-24
WO2008046922A3 true WO2008046922A3 (fr) 2008-06-12

Family

ID=38863097

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/061241 WO2008046922A2 (fr) 2006-10-20 2007-10-19 Méthode d'analyse des variations de séquence dans la zone génomique ns5b du vhc

Country Status (2)

Country Link
EP (1) EP2121990A2 (fr)
WO (1) WO2008046922A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2546190T3 (es) 2008-10-06 2015-09-21 Janssen Diagnostics Bvba Método para determinar mutaciones de resistencia a fármacos en cualquiera de las regiones de proteína no estructural NS3 a NS5B del virus de la hepatitis C (HCV) para los genotipos 1 a 6
CN113151595B (zh) * 2021-03-31 2024-02-06 广州金域医学检验中心有限公司 用于检测hcv 6型耐药突变基因的扩增引物、检测方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025601A2 (fr) * 1993-04-27 1994-11-10 N.V. Innogenetics S.A. Nouvelles sequences de genotypes du virus de l'hepatite c et leur utilisation en tant qu'agents therapeutiques et diagnostiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025601A2 (fr) * 1993-04-27 1994-11-10 N.V. Innogenetics S.A. Nouvelles sequences de genotypes du virus de l'hepatite c et leur utilisation en tant qu'agents therapeutiques et diagnostiques

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CHAYAMA KAZUAKI ET AL: "Genotypic subtyping of hepatitis C virus", JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, vol. 8, no. 2, 1993, pages 150 - 156, XP009080652, ISSN: 0815-9319 *
CHAYAMA KAZUAKI: "Genotyping hepatitis C virus by type-specific primers for PCR based on NS5 region", 1998, METHODS IN MOLECULAR MEDICINE, HUMANA PRESS, TOTOWA, NJ, US, PAGE(S) 165-173, ISSN: 1543-1894, XP009094859 *
DATABASE EMBL [online] 15 December 2005 (2005-12-15), "Sequence 84 from Patent EP1602736.", XP002426573, retrieved from EBI accession no. EMBL:CS227621 Database accession no. CS227621 *
MAY WANG Q ET AL: "Expression and purification of untagged full-length HCV NS5B RNA-dependent RNA polymerase", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 35, no. 2, June 2004 (2004-06-01), pages 304 - 312, XP004506567, ISSN: 1046-5928 *
OHNO T ET AL: "NEW HEPATITIS C VIRUS (HCV) GENOTYPING SYSTEM THAT ALLOWS FOR IDENTIFICATION OF HCV GENOTYPES 1A, 1B, 2A, 2B, 3A, 3B, 4, 5A, AND 6A", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 35, no. 1, 1997, pages 201 - 207, XP000911892, ISSN: 0095-1137 *
SANDRES-SAUNE K ET AL: "Determining hepatitis C genotype by analyzing the sequence of the NS5b region.", JOURNAL OF VIROLOGICAL METHODS, vol. 109, no. 2, May 2003 (2003-05-01), pages 187 - 193, XP002425272, ISSN: 0166-0934 *
SIMMONDS PETER ET AL: "Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes", HEPATOLOGY, vol. 42, no. 4, October 2005 (2005-10-01), pages 962 - 973, XP002423614, ISSN: 0270-9139 *
TOMEI LICIA ET AL: "HCV antiviral resistance: the impact of in vitro studies on the development of antiviral agents targeting the viral NS5B polymerase.", ANTIVIRAL CHEMISTRY & CHEMOTHERAPY 2005, vol. 16, no. 4, 2005, pages 225 - 245, XP009080813, ISSN: 0956-3202 *

Also Published As

Publication number Publication date
WO2008046922A2 (fr) 2008-04-24
EP2121990A2 (fr) 2009-11-25

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