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WO2007138568A3 - Dispositif pour expériences sous microscope sur des cellules vivantes non adhérentes avec renouvellement continu - Google Patents

Dispositif pour expériences sous microscope sur des cellules vivantes non adhérentes avec renouvellement continu Download PDF

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Publication number
WO2007138568A3
WO2007138568A3 PCT/IL2007/000570 IL2007000570W WO2007138568A3 WO 2007138568 A3 WO2007138568 A3 WO 2007138568A3 IL 2007000570 W IL2007000570 W IL 2007000570W WO 2007138568 A3 WO2007138568 A3 WO 2007138568A3
Authority
WO
WIPO (PCT)
Prior art keywords
experiments
interest
flow
cells
live cells
Prior art date
Application number
PCT/IL2007/000570
Other languages
English (en)
Other versions
WO2007138568A2 (fr
Inventor
Cher Ashtamker
Robert Fluhr
Original Assignee
Yeda Res & Dev
Cher Ashtamker
Robert Fluhr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev, Cher Ashtamker, Robert Fluhr filed Critical Yeda Res & Dev
Publication of WO2007138568A2 publication Critical patent/WO2007138568A2/fr
Publication of WO2007138568A3 publication Critical patent/WO2007138568A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Fluid Mechanics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un dispositif utilisé dans les applications de microscopie, et particulièrement celles utilisées pour examiner des spécimens contenant des cellules vivantes non adhérentes ou d'autres particules étudiées en suspension dans un milieu liquide, dans des expériences avec renouvellement continu. Ce dispositif comporte un réseau maillé ouvert se plaçant entre lame et lamelle de façon à recevoir un spécimen des cellules étudiées. De par sa construction et ses dimensions, ce réseau maillé ouvert, qui constitue une multitude de petites ouvertures de mailles définissant une matrice bidimensionnelle de pièges physiques reliés les uns aux autres de façon à recevoir le spécimen, est capable de limiter le déplacement des cellules étudiées à l'intérieur du réseau sans affecter de façon indue la viabilité des cellules étudiées, ou de limiter l'écoulement du milieu environnant, ce qui permet de conduire des expériences avec renouvellement continu.
PCT/IL2007/000570 2006-05-25 2007-05-10 Dispositif pour expériences sous microscope sur des cellules vivantes non adhérentes avec renouvellement continu WO2007138568A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80811106P 2006-05-25 2006-05-25
US60/808,111 2006-05-25

Publications (2)

Publication Number Publication Date
WO2007138568A2 WO2007138568A2 (fr) 2007-12-06
WO2007138568A3 true WO2007138568A3 (fr) 2009-04-23

Family

ID=38779079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2007/000570 WO2007138568A2 (fr) 2006-05-25 2007-05-10 Dispositif pour expériences sous microscope sur des cellules vivantes non adhérentes avec renouvellement continu

Country Status (1)

Country Link
WO (1) WO2007138568A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201121959D0 (en) * 2011-12-21 2012-02-01 Univ Leeds Assay of functional cell viability
CN103105670A (zh) * 2013-02-06 2013-05-15 李宏 镜下观察用细胞载片
KR20170099737A (ko) * 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 이를 이용하는 염색 방법
CN109351377A (zh) * 2018-11-28 2019-02-19 浙江警察学院 一种内设交叉阵列可单向拦截杂质的微流控芯片

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3768914A (en) * 1972-09-18 1973-10-30 T Kinney Electron microscopy tissue grid staining and storing rack and method
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5792653A (en) * 1991-09-28 1998-08-11 Kernforschungszenlrum Karlsruhe Gmbh Substrate for cell structures

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3768914A (en) * 1972-09-18 1973-10-30 T Kinney Electron microscopy tissue grid staining and storing rack and method
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5792653A (en) * 1991-09-28 1998-08-11 Kernforschungszenlrum Karlsruhe Gmbh Substrate for cell structures

Also Published As

Publication number Publication date
WO2007138568A2 (fr) 2007-12-06

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