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WO2007039699A3 - Substrat proteique pour la detection de l'activite calpaine 3 - Google Patents

Substrat proteique pour la detection de l'activite calpaine 3 Download PDF

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Publication number
WO2007039699A3
WO2007039699A3 PCT/FR2006/050964 FR2006050964W WO2007039699A3 WO 2007039699 A3 WO2007039699 A3 WO 2007039699A3 FR 2006050964 W FR2006050964 W FR 2006050964W WO 2007039699 A3 WO2007039699 A3 WO 2007039699A3
Authority
WO
WIPO (PCT)
Prior art keywords
calpain
activity
detection
proteinaceous substrate
proteinaceous
Prior art date
Application number
PCT/FR2006/050964
Other languages
English (en)
Other versions
WO2007039699A2 (fr
Inventor
Isabelle Richard
Marc Bartoli
Original Assignee
Genethon
Centre Nat Rech Scient
Isabelle Richard
Marc Bartoli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genethon, Centre Nat Rech Scient, Isabelle Richard, Marc Bartoli filed Critical Genethon
Publication of WO2007039699A2 publication Critical patent/WO2007039699A2/fr
Publication of WO2007039699A3 publication Critical patent/WO2007039699A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22054—Calpain-3 (3.4.22.54), i.e. calpain p94
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention a trait à un substrat protéique comprenant une protéine calpaine 3 dépourvue d'activité autolytique et une étiquette de détection, ainsi qu'à une méthode de détection in vitro de l'activité calpaine 3 dans un échantillon biologique.
PCT/FR2006/050964 2005-09-30 2006-09-29 Substrat proteique pour la detection de l'activite calpaine 3 WO2007039699A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0552980 2005-09-30
FR0552980A FR2891544A1 (fr) 2005-09-30 2005-09-30 Substrat proteique pour la detection de l'activite calpaine 3

Publications (2)

Publication Number Publication Date
WO2007039699A2 WO2007039699A2 (fr) 2007-04-12
WO2007039699A3 true WO2007039699A3 (fr) 2007-05-24

Family

ID=36581666

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2006/050964 WO2007039699A2 (fr) 2005-09-30 2006-09-29 Substrat proteique pour la detection de l'activite calpaine 3

Country Status (2)

Country Link
FR (1) FR2891544A1 (fr)
WO (1) WO2007039699A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2962041B1 (fr) 2010-07-01 2012-07-27 Genethon Inhibiteurs de la calpaine 3 pour le traitement de dystrophies musculaires et de cardiomyopathies
EP3097197B1 (fr) 2014-01-21 2020-12-16 Vrije Universiteit Brussel Éléments régulateurs d'acide nucléique exprimé dans un muscle, méthodes et utilisation associées
ES2982489T3 (es) 2016-08-19 2024-10-16 Univ Florida Composiciones para el tratamiento de afecciones que utilizan virus adeno-asociados recombinantes autocomplementarios
CA3046347A1 (fr) 2016-12-07 2018-06-14 University Of Florida Research Foundation, Incorporated Adnc d'il-1ra

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003002730A2 (fr) * 2001-06-29 2003-01-09 Genethon Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003002730A2 (fr) * 2001-06-29 2003-01-09 Genethon Methode de detection de l'activite de la calpaine 3 dans un echantillon biologique et peptides pour la mise en oeuvre de ladite methode

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FANIN MARINA ET AL: "Loss of calpain-3 autocatalytic activity in LGMD2A patients with normal protein expression.", AMERICAN JOURNAL OF PATHOLOGY, vol. 163, no. 5, November 2003 (2003-11-01), pages 1929 - 1936, XP002386341, ISSN: 0002-9440 *
GUBITZ AMELIE K ET AL: "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 7, 15 February 2002 (2002-02-15), pages 5631 - 5636, XP002386342, ISSN: 0021-9258 *
ONO Y ET AL: "Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 273, no. 27, 3 July 1998 (1998-07-03), pages 17073 - 17078, XP002238594, ISSN: 0021-9258 *
YANG YI ET AL: "RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 277, no. 28, 12 July 2002 (2002-07-12), pages 25756 - 25774, XP002216975, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2007039699A2 (fr) 2007-04-12
FR2891544A1 (fr) 2007-04-06

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