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WO2007031158A1 - Dispositif de temperationde laboratoire comportant un cote superieur - Google Patents

Dispositif de temperationde laboratoire comportant un cote superieur Download PDF

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Publication number
WO2007031158A1
WO2007031158A1 PCT/EP2006/007814 EP2006007814W WO2007031158A1 WO 2007031158 A1 WO2007031158 A1 WO 2007031158A1 EP 2006007814 W EP2006007814 W EP 2006007814W WO 2007031158 A1 WO2007031158 A1 WO 2007031158A1
Authority
WO
WIPO (PCT)
Prior art keywords
adapter
vessels
tempering device
laboratory tempering
crystallization
Prior art date
Application number
PCT/EP2006/007814
Other languages
German (de)
English (en)
Inventor
Ben Hankamer
James Bond
Original Assignee
Eppendorf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Ag filed Critical Eppendorf Ag
Priority to EP06763011A priority Critical patent/EP1924361A1/fr
Publication of WO2007031158A1 publication Critical patent/WO2007031158A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/54—Heating or cooling apparatus; Heat insulating devices using spatial temperature gradients
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/54—Organic compounds
    • C30B29/58—Macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/06—Crystallising dishes

Definitions

  • the invention relates to a laboratory tempering referred to in the preamble of claim 1 Art.
  • Generic laboratory tempering devices are e.g. from US 5,525,300 A known. They are used to hold laboratory samples at certain temperatures. The laboratory samples are arranged in containers, which are arranged with parts of their outer surface in large-area thermal contact in the wells. It is also possible to arrange a plurality of vessels combined into a plate, so-called microtiter plates with their vessels projecting downwards from the plate into a plurality of wells of the laboratory tempering device, for which purpose the grid dimension of the wells must match the grid dimension of the vessels of the plate.
  • DE 19646115 A1 also shows a generic construction which, in an embodiment variant, has recesses of different sizes closely nested in the upper side, so that two different vessel sizes and two different patterns can be used. However, other vessel sizes and rasters are not possible here either.
  • the object of the present invention is to allow the use of different vessel sizes and plate grid in a generic Laborortemperier worn in a simple manner.
  • the laboratory tempering is provided with an adapter which is adapted to its underside of the upper side of the laboratory tempering to flat heat contact and transports the heat to its top, which is adapted to other vessels to flat heat contact.
  • an adapter which is adapted to its underside of the upper side of the laboratory tempering to flat heat contact and transports the heat to its top, which is adapted to other vessels to flat heat contact.
  • vessels or plates of a second type can be recorded in surface heat contact
  • the adapter is adapted on its underside of the top of the laboratory tempering, which in itself for completely different vessels or grid of a first type is formed.
  • the adapter could be configured to contact the top over the recesses with a simple flat bottom.
  • the features of claim 2 are provided.
  • the contact of the upper side of the laboratory tempering devices takes place in their depressions, in which projections on the underside of the adapter engage in good heat-conducting, large-area contact.
  • the heat transfer between the laboratory tempering device and the adapter takes place at the points of the recesses in which the set target temperatures are maintained particularly accurately.
  • the laboratory tempering device generates a temperature gradient, advantageously according to claim 5, transversely to the lines.
  • the rows are thus at different temperatures, which are transmitted separately upwards by the adapters arranged line by line.
  • the vessels of the second type which contact the top of the adapters, are also brought line by line to different temperatures according to the set gradient.
  • crystallization plates are increasingly being used, in whose vessels crystallization processes take place.
  • crystallization plates are used for the determination of proteins by X-ray diffraction on a lattice produced by crystallization, eg in genetic research.
  • suitable laboratory tempering devices for crystallization plates however, their exact temperature control has hitherto been very problematic.
  • Crystallization plates are commercially available in a wide variety of forms, e.g. depending on the crystallization method, e.g. "Hanging Drop” or “Sitting Drop”.
  • the crystallization vessels are easier with the "hanging drop” method and somewhat more complicated with the “sitting drop” method.
  • crystallization plates with closely nested crystallization vessels and those with a common base plate down individually in the lateral distance hanging crystallization vessels.
  • the features of claim 7 are advantageously provided, wherein the crystallization vessels are laterally encompassed by the wells of the adapter, so that temperature differences within the crystallization vessel, which could interfere with the Kritallisationslui be avoided.
  • FIG. 1 shows a section through a laboratory tempering device in a first
  • Fig. 5 is a plan view of the adapter in section along line 5 - 5 in
  • Fig. 6 is a perspective view of an adapter in a third embodiment.
  • the tempering elements 5 are, for example, Peltier elements which are supplied with electricity via lines which are not shown and which can heat or cool the block 3 as desired. If the plurality of tempering elements 5 arranged one behind the other in the longitudinal direction are operated at different temperatures, a temperature gradient can be set in the direction of the arrow 6, ie in the longitudinal direction of the upper side 2 in the direction of the arrow 6.
  • Recesses 7 are formed in the upper side 2 of the laboratory tempering device 1 and are arranged, for example, in rows and columns, as is known from the documents cited at the beginning.
  • they serve vessels of a first type, ie, for example, containing individual vessels containing reaction samples in a large area of good heat-conducting contact, or in the grid (eg 12 rows and 8 columns) suitable microtiter plates, in which an upper surface hanging vessels fit into the recesses 7.
  • vessels of a different, second type namely vessels of a crystallization plate 8 are tempered with the laboratory tempering device 1, which is shown in section in FIG. 1 and in plan view in FIG. 3.
  • the crystallization plate 8 is a crystallization plate with vessels designed for the "sitting drop” method.
  • the crystallization plate 8 has for this purpose formed in a grid crystallization vessels 9, which are divided laterally by crossing continuous partitions 10, are open at the top and have a step 11, are placed on the drops with a protein solution to be crystallized, while in the lower Part of the vessel 9 solvent is arranged.
  • all the crystallization vessels 9 are closed with a covering the entire crystallization plate 8 cover 12, as shown in Fig. 1.
  • the closure can be done with an adhesive film.
  • the crystallization plate 8 forms on its side facing the laboratory tempering 1 underside under the steps 11 in the direction perpendicular to the plane of the drawing of FIG. 1 extending, rectangular, downwardly open grooves 13.
  • a precise temperature control of the crystallization vessels 9 of the crystallization plate 8 by placing it on the top side 2 of the laboratory tempering device 1 would be difficult because of insufficient contact surfaces.
  • the bars 15 can thus be used with all-sided large-area and thus good heat-conducting surface contact in the grooves 13.
  • the adapters 14 are provided with projections 16 projecting downwards, which, like FIG. 1, are adapted in their surface form exactly to the inner surface of the depressions 7 in the upper side 2 of the laboratory temperature control device 1.
  • a plurality of elevations 16 are provided on an adapter 14, which are arranged linearly in a row which corresponds to the grid of depressions 7 in a row of the upper side 2 of the laboratory tempering device 1.
  • Fig. 1 shows that the adapters 14 are inserted with their elevations 16 in the recesses 7 and are used with their beams 15 in the grooves 13 of the crystallization plate 8.
  • the crystallization plate 8 is therefore heated over a large area and precisely via the adapters 14, which for this purpose are made of material which conducts heat well, e.g. Metal are formed, wherein it is particularly important to transport the heat in the direction perpendicular to the plane of the top 2 of the laboratory tempering 1.
  • FIGS. 3a to 5c describe crystallization plates of another embodiment, which are essentially derived from the Crystallization plate 8 differ in that the crystallization vessels have no common partitions, but hang down freely in a lateral distance substantially downwards. Also, such a crystallization plate can be easily supplied with the present invention, as shown in FIGS. 4 and 5 show.
  • FIG. 4 shows the same laboratory tempering device 1 with recesses 7 as in FIG. 1.
  • Adapters 44 which largely correspond to the adapters 14, that is to say have elevations 46 which correspond to the depressions 7 in the grid and the shaping, are inserted into these are adapted.
  • the adapters 44 are again designed as bars 45, which in the embodiment of FIGS. 4 and 5, however, have depressions 47 which are formed in a shape-matched manner to the outer surface of the crystallization vessels 49 of a crystallization plate 48.
  • Fig. 6 shows in an embodiment variant of an adapter 64, which consists of individual adapters 44 of FIG. 5, which are placed side by side parallel to each other standing in the embodiment with a release layer 65 of thermally insulating material.
  • the adapter 44 of FIG. 4 can be assembled to form a plate-shaped adapter 64, which can be handled as a whole. If the adapter 64 is used on a laboratory tempering device 1 according to FIG. 1, in which the gradient 6 is generated in the direction transverse to the longitudinal extension of the individual adapters 44, then the thermally insulating separating layer 65 ensures that the desired different temperatures in the individual adapters 44 can be adjusted trouble-free.
  • the adapter 64 which is shown in Fig. 6, and the separating layer 65 can be omitted, so that the adapters is formed in its upper part as a continuous plate made of thermally conductive material. He would be in this form for Laboratory tempering suitable to generate over its top 2 across a uniform temperature. However, such an embodiment of an adapter would also be suitable when using a temperature gradient, which would then also be generated by appropriate heat flow in the adapter 64.
  • a respective row of wells 7 serving adapter 14 (Fig. 1) or 44 (Fig. 4) is used, then connected to the top of the adapter vessels in their arrangement grid in the direction transverse to the extension of the adapter to the grid of the recesses 7 in the laboratory tempering 1 fit.
  • the vessels adapted above may have a different grid than the depressions 7. This applies, for example, to the example shown in FIGS. 1 and 3, in which the grid of the crystallization vessels 9 in the direction of the arrow 6 must coincide with the grid of the recesses 7, may differ transversely thereto.
  • an adapter used with a continuous plate which engages with elevations 46 in the recesses 7 of FIG. 1 may be arranged in any desired pattern on the upper side of the adapter , which completely deviates from the grid of the wells 7.
  • the vessel shapes to be contacted above can be completely different from the vessel shape, which fits into the wells 7. So it can be e.g. to a laboratory tempering device with wells in 8x12 grid via a suitably designed adapter a microtiter plate are adapted with 16x24 grid.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un dispositif de températion de laboratoire (1) comportant un côté supérieur (2) pouvant être amené à des températures définies, présentant des cavités (7) adaptées à des contenants d'un premier type. Le dispositif selon l'invention est caractérisé en ce qu'il comporte un adaptateur (14, 44, 64) perpendiculaire au plan du côté supérieur (2) du dispositif (1), thermoconducteur, adapté sur son côté inférieur au côté supérieur (2) du dispositif (1) de manière à entrer en contact plan avec celui-ci, et adapté sur son côté supérieur au contact plan avec des contenants (9, 49) d'un deuxième type.
PCT/EP2006/007814 2005-09-14 2006-08-08 Dispositif de temperationde laboratoire comportant un cote superieur WO2007031158A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06763011A EP1924361A1 (fr) 2005-09-14 2006-08-08 Dispositif de temperationde laboratoire comportant un cote superieur

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200510044021 DE102005044021A1 (de) 2005-09-14 2005-09-14 Labortemperiereinrichtung mit Oberseite
DE102005044021.5 2005-09-14

Publications (1)

Publication Number Publication Date
WO2007031158A1 true WO2007031158A1 (fr) 2007-03-22

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ID=37000122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/007814 WO2007031158A1 (fr) 2005-09-14 2006-08-08 Dispositif de temperationde laboratoire comportant un cote superieur

Country Status (3)

Country Link
EP (1) EP1924361A1 (fr)
DE (1) DE102005044021A1 (fr)
WO (1) WO2007031158A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104114282B (zh) * 2011-09-30 2017-07-04 生命技术公司 用于生物分析的系统和方法
US10471432B2 (en) 2015-12-22 2019-11-12 Life Technologies Corporation Thermal cycler systems and methods of use

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061630A (en) * 1988-05-13 1991-10-29 Agrogen Foundation, Seyffer & Co. & Ulrich C. Knopf Laboratory apparatus for optional temperature-controlled heating and cooling
EP0611598A2 (fr) * 1993-02-16 1994-08-24 The Perkin-Elmer Corporation Procédé d'amplification PCR in situ
US5459300A (en) * 1993-03-03 1995-10-17 Kasman; David H. Microplate heater for providing uniform heating regardless of the geometry of the microplates
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
US20020114734A1 (en) * 1996-05-09 2002-08-22 Pantoliano Michael W. Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
US20020141905A1 (en) * 2001-03-19 2002-10-03 Corning Incorporated Microplate for performing crystallography studies and methods for making and using such microplates
WO2004018105A1 (fr) * 2002-08-20 2004-03-04 Quanta Biotech Limited Moteur thermique pour thermocycleur presentant un bloc d'echantillons interchangeable

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061630A (en) * 1988-05-13 1991-10-29 Agrogen Foundation, Seyffer & Co. & Ulrich C. Knopf Laboratory apparatus for optional temperature-controlled heating and cooling
EP0611598A2 (fr) * 1993-02-16 1994-08-24 The Perkin-Elmer Corporation Procédé d'amplification PCR in situ
US5459300A (en) * 1993-03-03 1995-10-17 Kasman; David H. Microplate heater for providing uniform heating regardless of the geometry of the microplates
US20020114734A1 (en) * 1996-05-09 2002-08-22 Pantoliano Michael W. Microplate thermal shift assay apparatus for ligand development and multi-variable protein chemistry optimization
DE19646115A1 (de) * 1996-11-08 1998-05-14 Eppendorf Geraetebau Netheler Temperierblock mit Temperiereinrichtungen
US20020141905A1 (en) * 2001-03-19 2002-10-03 Corning Incorporated Microplate for performing crystallography studies and methods for making and using such microplates
WO2004018105A1 (fr) * 2002-08-20 2004-03-04 Quanta Biotech Limited Moteur thermique pour thermocycleur presentant un bloc d'echantillons interchangeable

Also Published As

Publication number Publication date
DE102005044021A1 (de) 2007-03-15
EP1924361A1 (fr) 2008-05-28

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