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WO2007016665A3 - Dosages par fluorescence a usage unique servant a determiner des substances a analyser - Google Patents

Dosages par fluorescence a usage unique servant a determiner des substances a analyser Download PDF

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Publication number
WO2007016665A3
WO2007016665A3 PCT/US2006/030244 US2006030244W WO2007016665A3 WO 2007016665 A3 WO2007016665 A3 WO 2007016665A3 US 2006030244 W US2006030244 W US 2006030244W WO 2007016665 A3 WO2007016665 A3 WO 2007016665A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
reagent
fluorescence
nanocrystals
fluid sample
Prior art date
Application number
PCT/US2006/030244
Other languages
English (en)
Other versions
WO2007016665A2 (fr
Inventor
Frank A Fornari
Stephen Jordon
Original Assignee
Dominion Diagnostics Llc
Frank A Fornari
Stephen Jordon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dominion Diagnostics Llc, Frank A Fornari, Stephen Jordon filed Critical Dominion Diagnostics Llc
Publication of WO2007016665A2 publication Critical patent/WO2007016665A2/fr
Publication of WO2007016665A3 publication Critical patent/WO2007016665A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Materials Engineering (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un système de dosage par fluorescence à usage unique, qui sert à déterminer la présence d'au moins une substance à analyser dans un échantillon de liquide, notamment un échantillon de liquide biologique. Le système de dosage comprend un premier réactif contenant des nanocristaux fluorescents, et un second réactif contenant un fragment de désactivation qui, dans au moins une situation prédéterminée, se lie efficacement au premier réactif pour désactiver la fluorescence des nanocristaux. La situation prédéterminée peut être l'absence de la substance à analyser dans un échantillon de liquide ou, dans un autre mode de réalisation, la présence de la substance à analyser dans l'échantillon de liquide. Certains modes de réalisation exemplaires mettent en oeuvre un boîtier, et un élément de dosage à usage unique configuré pour s'associer au boîtier, recevoir un échantillon de liquide et doser au moins une substance à analyser présumée présente dans l'échantillon de liquide. Le système de dosage par fluorescence à usage unique comprend en outre, facultativement, un fluormètre pouvant générer un éclairage d'excitation au niveau d'un site de détection pour provoquer la fluorescence des nanocristaux dans la situation de non extinction. Dans certains modes de réalisation du système de dosage par fluorescence à usage unique, un premier réactif comprend des nanocristaux fluorescents comportant un ou plusieurs sites de liaison de la substance à analyser cible, et un second réactif comprend la substance à analyser cible ou un analogue de celle-ci conjugué à des fragments de désactivation.
PCT/US2006/030244 2005-07-29 2006-07-28 Dosages par fluorescence a usage unique servant a determiner des substances a analyser WO2007016665A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US70414305P 2005-07-29 2005-07-29
US60/704,143 2005-07-29
US70425205P 2005-08-01 2005-08-01
US60/704,252 2005-08-01

Publications (2)

Publication Number Publication Date
WO2007016665A2 WO2007016665A2 (fr) 2007-02-08
WO2007016665A3 true WO2007016665A3 (fr) 2007-06-28

Family

ID=37709363

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/030244 WO2007016665A2 (fr) 2005-07-29 2006-07-28 Dosages par fluorescence a usage unique servant a determiner des substances a analyser

Country Status (1)

Country Link
WO (1) WO2007016665A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5089705B2 (ja) 2008-11-20 2012-12-05 アークレイ株式会社 光学的測定装置
DE102014203266B4 (de) 2014-02-24 2018-07-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zusammensetzung mit FRET-Paar in definierter Geometrie
EE01561U1 (et) * 2019-02-11 2022-03-15 Qanikdx Oü Meetod vedelas proovis vähemalt ühe orgaanilise analüüdi tuvastamiseks ja kvantifitseerimiseks.
CN111077001B (zh) * 2020-01-12 2022-05-10 天津市宝坻区人民医院 抗血小板抗体生物薄片的制作方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020004246A1 (en) * 2000-02-07 2002-01-10 Daniels Robert H. Immunochromatographic methods for detecting an analyte in a sample which employ semiconductor nanocrystals as detectable labels

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020004246A1 (en) * 2000-02-07 2002-01-10 Daniels Robert H. Immunochromatographic methods for detecting an analyte in a sample which employ semiconductor nanocrystals as detectable labels

Also Published As

Publication number Publication date
WO2007016665A2 (fr) 2007-02-08

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