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WO2007063129A3 - Isolement de peptides, polypeptides et protéines - Google Patents

Isolement de peptides, polypeptides et protéines Download PDF

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Publication number
WO2007063129A3
WO2007063129A3 PCT/EP2006/069208 EP2006069208W WO2007063129A3 WO 2007063129 A3 WO2007063129 A3 WO 2007063129A3 EP 2006069208 W EP2006069208 W EP 2006069208W WO 2007063129 A3 WO2007063129 A3 WO 2007063129A3
Authority
WO
WIPO (PCT)
Prior art keywords
insolation
polypeptides
peptides
proteins
protein
Prior art date
Application number
PCT/EP2006/069208
Other languages
English (en)
Other versions
WO2007063129A2 (fr
Inventor
Bjarke Christensen
Allan Noergaard
Original Assignee
Novozymes As
Upfront Chromatography As
Bjarke Christensen
Allan Noergaard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Upfront Chromatography As, Bjarke Christensen, Allan Noergaard filed Critical Novozymes As
Publication of WO2007063129A2 publication Critical patent/WO2007063129A2/fr
Publication of WO2007063129A3 publication Critical patent/WO2007063129A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/473—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used alpha-Glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé d'isolement de rHSA d'une solution protéique. Le procédé comprend les étapes consistant: a) à utiliser une solution protéique comprenant une ou plusieurs protéines spécifiques et possédant un pH établi au préalable et une force ionique ou une conductivité établie au préalable, b) à faire passer la solution protéique dans une colonne à lit fixe ou expansé comprenant un adsorbant, et c) à obtenir une ou plusieurs protéines à partir de la colonne.
PCT/EP2006/069208 2005-12-02 2006-12-01 Isolement de peptides, polypeptides et protéines WO2007063129A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200501713 2005-12-02
DKPA200501713 2005-12-02
DKPA200501738 2005-12-07
DKPA200501738 2005-12-07

Publications (2)

Publication Number Publication Date
WO2007063129A2 WO2007063129A2 (fr) 2007-06-07
WO2007063129A3 true WO2007063129A3 (fr) 2007-07-12

Family

ID=37770284

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/069208 WO2007063129A2 (fr) 2005-12-02 2006-12-01 Isolement de peptides, polypeptides et protéines

Country Status (1)

Country Link
WO (1) WO2007063129A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106661587A (zh) * 2014-01-31 2017-05-10 法纳生物解决办法有限责任公司 Crm197和相关蛋白的表达和纯化

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008289543B2 (en) * 2007-08-17 2011-11-03 Csl Behring Gmbh Methods for purification of alpha-1-antitrypsin and apolipoprotein A-I
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
CN102533553B (zh) * 2011-12-29 2013-06-26 湖南省微生物研究所 一种快速降解稻草秸秆的有机物料腐熟组合菌剂及其应用方法
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
US11060123B2 (en) 2014-01-31 2021-07-13 Fina Biosolutions, Llc Production of soluble recombinant protein without n-terminal methionine
US12104161B2 (en) 2014-01-31 2024-10-01 Fina Biosolutions Llc Production of soluble recombinant proteins without N-terminal methionine in E-coli
CN109182145B (zh) * 2018-09-29 2021-05-11 武汉友芹种苗技术有限公司 一种棘孢曲霉菌株及其应用
EP4476238A1 (fr) * 2022-02-11 2024-12-18 Clara Foods Co. Compositions protéiques et produits de consommation s'y rapportant

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0699687A2 (fr) * 1994-08-31 1996-03-06 The Green Cross Corporation Procédé de purification du sérum albumine humaine
WO2000044772A2 (fr) * 1999-01-30 2000-08-03 Delta Biotechnology Limited Technique
WO2005121163A2 (fr) * 2004-06-07 2005-12-22 Upfront Chromatography A/S Isolation de proteines du plasma ou du serum

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0699687A2 (fr) * 1994-08-31 1996-03-06 The Green Cross Corporation Procédé de purification du sérum albumine humaine
WO2000044772A2 (fr) * 1999-01-30 2000-08-03 Delta Biotechnology Limited Technique
WO2005121163A2 (fr) * 2004-06-07 2005-12-22 Upfront Chromatography A/S Isolation de proteines du plasma ou du serum

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GOUKA R J ET AL: "Efficient production of secretedproteins by Aspergillus: progress, limitations and prospects", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 47, no. 1, January 1997 (1997-01-01), pages 1 - 11, XP002121584, ISSN: 0175-7598 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106661587A (zh) * 2014-01-31 2017-05-10 法纳生物解决办法有限责任公司 Crm197和相关蛋白的表达和纯化

Also Published As

Publication number Publication date
WO2007063129A2 (fr) 2007-06-07

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