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WO2006136990A2 - Cartouche, systeme et procede pour diagnostic medical automatique - Google Patents

Cartouche, systeme et procede pour diagnostic medical automatique Download PDF

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Publication number
WO2006136990A2
WO2006136990A2 PCT/IB2006/051941 IB2006051941W WO2006136990A2 WO 2006136990 A2 WO2006136990 A2 WO 2006136990A2 IB 2006051941 W IB2006051941 W IB 2006051941W WO 2006136990 A2 WO2006136990 A2 WO 2006136990A2
Authority
WO
WIPO (PCT)
Prior art keywords
sample
cartridge
detection
cartridge according
amplification
Prior art date
Application number
PCT/IB2006/051941
Other languages
English (en)
Other versions
WO2006136990A3 (fr
Inventor
Chris Van Haag
Michiel J. Jongerius
Danny G. A. Schaefer
Adrianus W. D. M. Van Den Bijgaart
Ronald C. De Gier
Michiel De Jong
Gerhard Pross
Johannes Bacher
Andreas Boos
Gerd Luedke
Jens-Peter Seher
Original Assignee
Koninklijke Philips Electronics N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37188981&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2006136990(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Koninklijke Philips Electronics N.V. filed Critical Koninklijke Philips Electronics N.V.
Priority to EP11005261.0A priority Critical patent/EP2409767B1/fr
Priority to EP06765771A priority patent/EP1896180B1/fr
Priority to US11/917,955 priority patent/US9568424B2/en
Priority to AT06765771T priority patent/ATE534465T1/de
Priority to CN2006800224237A priority patent/CN101203312B/zh
Priority to JP2008517654A priority patent/JP5086250B2/ja
Priority to ES06765771T priority patent/ES2373686T3/es
Publication of WO2006136990A2 publication Critical patent/WO2006136990A2/fr
Publication of WO2006136990A3 publication Critical patent/WO2006136990A3/fr
Priority to US15/387,096 priority patent/US20170100719A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/54—Labware with identification means
    • B01L3/545—Labware with identification means for laboratory containers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

Definitions

  • the invention pertains to a cartridge for the detection of the presence, absence or amount of specific DNA or RNA sequences.
  • the invention also pertains to the use of a system, optionally incorporating a cartridge, for the detection of the presence, absence or amount of specific DNA or RNA sequences. Since the discovery of DNA, the technology relating to the detection of the presence, absence or amount of specific DNA or RNA sequences in a sample has taken an enormous flight. Especially PCR, the Polymerase Chain Reaction has contributed enormously to the development of assays of all types for the detection of the presence or absence of DNA or RNA sequences. At present, it is possible to collect DNA containing samples from an organism and determine the presence, absence or amount therein of certain specific DNA sequences (target sequences). Technology is available to perform such analysis for multiple target sequences at the same time, so-called multiplex detection of target sequences to thereby increase throughput.
  • an efficient procedure for a conventional DNA or RNA analysis takes about 6 hours due to, inter alia, all the handling between the various systems for the taking of samples, the isolation of DNA or RNA from the sample, the subsequent assay for the analysis of the presence, absence or amount of the target sequence in the sample, the processing of any results obtained and the corresponding presentation of the results.
  • Cartridge-based systems for the detection of DNA have been disclosed before.
  • one of the one or more specific parts is a PCR body having one or more thermocycling chambers and comprising a number of primers. It may be possible that the cartridge is to be used for different panels of bacteria/resistances. By providing different PCR bodies comprising a number of primers, an application-specific PCR body can be chosen on the basis of the application for which the cartridge is used. For instance the PCR body comprising the primers can be selected on the basis of the panels of bacteria/resistances that are to be detected, which selection may be specific for a particular assay or for a particular region, such as Europe, Asia or Africa.
  • the cartridge may be of an exchangeable type which can be positioned in a reusable apparatus. Such cartridge may be disposable, recyclable or reusable, possibly after cleaning. By providing an exchangeable cartridge all parts that may come into contact with the sample may after the detection process be taken out of the apparatus and the cartridge may be exchanged for another one or cleaned before a next use. In other embodiments the cartridge may be an integral part of the reusable apparatus which is cleaned after each use.
  • the system can also comprise the means for the introduction of one or more samples.
  • sample introduction means may comprise any suitable device, such as a holding or docking device for the introduction of a sample from a syringe or pipette or such and may for instance comprise a one-way inlet valve, a septum, filters, and an overflow.
  • the target nucleotide sequence can be selected from the group consisting of DNA, genomic DNA, RNA, mRNA, cDNA, transgenic DNA, ETC.
  • the amplification step is preceded or replaced by an assay for the detection of nucleic acids in samples.
  • Fig. 1 shows a perspective view of a system according to an embodiment of the invention
  • Fig. 2 shows a schematic block diagram of the architecture of an embodiment of the system according to the invention
  • the cartidge 5 comprises introduction means for the introduction of a sample, isolation means for the isolation of DNA, amplification means for the amplification of DNA, and detection means for the detection of amplified DNA.
  • the introduction means, isolation means, amplification means and/or detection means may be arranged on the cartridge and/or in the reusable apparatus. In general it is preferred to arrange in the apparatus 2 all parts of the system 1 which normally do not come into contact with the sample. The sample is held throughout the detection process in cartridge which works as a cartridge.
  • a preferred embodiment of the arrangement of the introduction means, isolation means, amplification means and/or detection means is described. However, other embodiments are also possible.
  • the apparatus may further comprise a data collection device and a data processing device to collect data obtained from the detection device and to process these data, respectively.
  • the apparatus 2 comprises a carrier 6 for supporting the cartridge 5.
  • the carrier 6 is movable in a vertical direction between a lower position (in which the carrier is shown) and a higher position. In the lower position the cartridge 5 can be placed on or taken from the carrier 6.
  • the higher position is the working position in which the cartridge 5 is positioned during the detection process. In this higher position the cartridge is clamped between the carrier 6 and the a number of devices being arranged on the cartridge, such as pump means, valves, mechanical means, and a detection chamber may cooperate with corresponding devices being arranged in the apparatus 2, such as pump means, valve and other mechanical actuation devices, and a detection device.
  • a part of the apparatus 2 comprising the corresponding devices can be moved towards and away from a cartridge placed in the apparatus 2.
  • FIG 2 a schematic block diagram is shown in which the different process steps of the detection process using the method according the present invention are shown. This diagram is used to explain the main architecture of the cartridge 5 and the relation between the apparatus 2 and the cartridge 5.
  • introduction chamber and lysis chamber are the same chamber.
  • the lysis device comprises a physical or mechanical manipulation means for the lysis step.
  • chemical means can be used for lysis of the cells in the sample, such as a lysis buffer.
  • lysis buffer may be held before use in a separate lysis buffer container which is in fluid communication with the lysis chamber.
  • a valve preferably a one-way valve, may be provided in the fluid channel connecting the lysis buffer container and the lysis chamber.
  • Means for mixing can be provided to mix the sample and the lysis buffer. These mixing means may be actuated by the apparatus.
  • the enrichment step is carried out in an enrichment chamber which is in fluid communication with the lysis chamber.
  • a valve is provided in the fluid channel between lysis chamber and enrichment chamber to make it possible that only a flow through the fluid channel is possible when required.
  • the valve may be actuable by the valve actuation means provided in the apparatus.
  • a fifth step the total amount of DNA or RNA to be analyzed may be increased by the use of a pre-amplification device.
  • Subjecting DNA or RNA obtained from the isolation step to a pre-amplification step can increase the total amount of DNA. This is advantageously, especially in the case of multiplex analysis, where multiple tests are performed on the isolated DNA, for instance to detect the presence absence or amount of multiple pathogens in one sample at a time.
  • the pre-amplification device comprises a pre-amplification chamber in which the pre-amplification is carried out.
  • the preamplification chamber may be the same chamber as or a different chamber than the enrichment chamber and/or washing chamber.
  • the pre- amplification device is under the control of the control unit 7.
  • the pre-amplification device can be connected to a waste device for the disposal of materials.
  • amplification In a sixth step (“amplification") the isolated DNA, optionally pre-treated as described herein elsewhere, is subjected in the amplification device to an amplification treatment.
  • the amplification treatment comprises bringing the isolated DNA in contact with a set of PCR primers that are specific for the target nucleic acid, PCR enzymes such as one or more polymerases and dNTPs.
  • the amplification device comprises a plurality of amplification chambers.
  • the plurality of amplification chambers enables the isolated or pre- amplified DNA or RNA to be divided in portions and distributed amongst the chambers.
  • an amplification step can be performed using a different set of primers.
  • multiplex analysis is provided in that one sample can be analyzed for the presence, absence or amount of different target nucleic acids.
  • the primer set for each target nucleic acid can be equipped with a detectably different label, i.e. with a different fluorescent spectrum.
  • the cartridge may comprise reagents containers for holding reagents for the amplification of the isolated DNA such as enzymes, DNTPs etc.
  • the detection device can be under the control of the control unit 7.
  • the detection device may detect based on label, length, mobility, nucleotide sequence, mass or a combination thereof.
  • a detection device can detect based on optical, electrochemical, magnetic or mobility (gel-electrophoresis). In principle any suitable detection device known from prior art may be used.
  • the detected information may be collected by data collection means and processed by data processing means to come for instance to a certain diagnose.
  • the pump means in the cartridge are actuated by the pump means actuation devices provided in the apparatus 2. These pump means actuation devices are under control of the control unit 7.
  • valves may be provided to only allow a flow when required. As most fluid will pass the fluid channels only in one direction the valves are preferably one-way valves.
  • valves may be actuated by valve actuation devices which preferably are arranged in the apparatus 2.
  • the different parts of the cartridge 10 will hereinafter be described in the order in which they will be used when a detection method for detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences is performed.
  • the first application-specific part which is comprised in the cartridge 10 is a pre-lysis device 12. This pre-lysis device 12 is configured to process a sample to a certain state which can be processed by the cartridge 10.
  • the sample may be provided in a solid state, for instance dried out blood, while the cartridge is designed to process a sample in a fluid state.
  • the sample has to be brought into a fluid state before it can be processed in the cartridge.
  • processing may be performed by providing suitable enzymes in a suitable medium in the pre- lysis device 12.
  • suitable enzymes such as for example trypsinization.
  • trypsinization By providing a pre-lysis device which can be connected to the generic part 11, the processing of the sample to the desired state can be performed without the need of transferring the sample after processing thereof therewith avoiding any chance on contamination.
  • the processing of the sample to the desired state may be performed before or after that the pre-lysis device is connected to the generic part 11.
  • the pre-lysis-device may also be indicated as a sample introduction device.
  • the sample introduction device is then used to introduce the sample into the cartridge without risking any contamination, as the sample introduction device is designed to be connected to the generic part 11 for the introduction of the sample in the cartridge 10.
  • the generic part 11 of the cartridge 10 comprises fluid handling means including pumps and valves for pumping the sample to the different process chambers.
  • the generic part 11 comprises two main components 14, 15 which are placed against each other with interposition of a flexible membrane 16.
  • the two main components 14, 15 comprise recesses which together with the flexible membrane 16 may form pump chambers, valves, fluid channels, fluid storage stations and such.
  • the sample will mainly be kept above the flexible membrane, while pumps 17 and valves 18 are mainly actuated from the bottom side of the flexible membrane 16.
  • Fluid can be pumped in or out of a chamber by moving the flexible membrane to increase or decrease the space within the chamber, respectively.
  • the flexible membrane can for example be moved by introducing air or fluid into the space between the flexible membrane 16 and the component 15. The air or fluid may be introduced through the channels 19.
  • the other pump chambers may also be used as pump chambers in a corresponding way.
  • Other means for moving the flexible membrane such as mechanical actuators may also be used.
  • the valves may be actuated by air or fluid pressure, mechanical actuation or any other suitable actuation device.
  • the movement of the flexible membrane 16 with respect to the component 14 may also be used to open and close a valve seat, whereby for example in the closed position of a valve the flexible membrane 16 is held against a channel end of the component 14.
  • a lysis storage 20 is provided to store a lysis buffer before it is pumped in the lysis chamber.
  • the sample may be pumped to a second process chamber 21 wherein the sample may be enriched in accordance with step 3 and washed and purified in accordance with step 4 as described hereabove.
  • Fluid storages 22 are provided for the storage of different washing and purifying buffers which may be used during the washing and purifying steps. These fluid storages 22 are in fluid communication via valves with the second process chamber 21. After possible pre-amplification (as described in step 6 in realtion to figure 2) which may also be performed in the second process chamber 21 or in the chamber 23, the sample may be introduced in the PCR body 24.
  • This PCR body 24 is a second application-specific part of the cartridge.
  • the PCR body 24 is circular, disc shaped and connected with a click- fit connection 25 to the generic part 11.
  • the PCR body 25 comprises six thermocycling chambers 26 so that six PCR processes can be simultaneously be performed on the sample. Such PCR amplification process is hereabove described as step 6 in relation to figure 2.
  • Each of the thermocycling chambers 26 is provided with at least one specific primer.
  • the PCR body 25 may be selected out of a group of different types of PCR bodies each comprising a different set of primers, a different number of chambers and/or a different chamber size or geometry.
  • the PCR body comprising the primers can be selected on the basis of the panels of bacteria/resistances that are to be detected, which selection may be specific for a particular assay or for a particular region, such as Europe, Asia or Africa.
  • the primers are spotted on a wall of the thermocycling chambers, for instance by an inkjet printing method, so that during storage of the PCR bodies no special measures have to be taken to avoid that the primers flow out of the PCR body, which would for instance be the case if primers in a fluid state would be used.
  • a seal or separate sealed chamber may be provided for holding the primers any other application-specific fluid before use thereof.
  • a detection device may be chosen out a series of different application-specific detection devices which may be specially designed for each respective detection method.
  • the type of detection device that will be used in the cartridge 11 will be dependent on the type of PCR body which is used for the amplification process. Then the choice of a PCR body will automatically lead to a choice of the detection device.
  • the invention may also be used for specific parts of the cartridge which have to be pre-treated or have to be kept at a certain temperature which is not desired or required for the other parts of the cartridge.
  • a separate fluid container which can be used in the pre-treatment or stored at a different location, and which can consequently be connected to the generic part of the cartridge before use, may be very useful since the risk on contamination of that part, in particular the fluid therein is avoided, since the fluid does not have to be transferred from a container to the cartridge in an open environment.
  • Such use of a separate part is regarded to be application-specific within the meaning of the present invention, even if the same part is used in a number of different applications.
  • An example of such separate part is a separate fluid container for a so-called PCR master mix which has to be stored at a low temperature before use on the cartridge.
  • the separate fluid container is connected to the generic part of the cartridge, for example by a click fit connection.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne une cartouche de recherche et de quantification d'une séquence nucléotide cible dans un échantillon comprenant au moins une séquence d'acide nucléique. La cartouche comprend une partie générique et une ou plusieurs parties distinctes, spécifiques de l'application, se reliant à la partie générique. L'invention concerne également un système de recherche et de quantification d'une séquence nucléotide cible dans un échantillon comprenant au moins une séquence d'acide nucléique. Ce système comprend un appareil réutilisable configuré pour recevoir une cartouche, et pour commander un processus de recherche et de quantification d'une séquence nucléotide cible dans un échantillon présent dans la cartouche de l'invention.
PCT/IB2006/051941 2005-06-23 2006-06-16 Cartouche, systeme et procede pour diagnostic medical automatique WO2006136990A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP11005261.0A EP2409767B1 (fr) 2005-06-23 2006-06-16 Cartouche modulaire, système et procédé de diagnostic médical automatique
EP06765771A EP1896180B1 (fr) 2005-06-23 2006-06-16 Cartouche, systeme et procede pour diagnostic medical automatique
US11/917,955 US9568424B2 (en) 2005-06-23 2006-06-16 Cartridge, system and method for automated medical diagnostics
AT06765771T ATE534465T1 (de) 2005-06-23 2006-06-16 Kartusche, system und verfahren für automatisierte medizinische diagnosen
CN2006800224237A CN101203312B (zh) 2005-06-23 2006-06-16 用于自动医疗诊断的盒体、系统和方法
JP2008517654A JP5086250B2 (ja) 2005-06-23 2006-06-16 自動医療診断用のカートリッジ、システム及び方法
ES06765771T ES2373686T3 (es) 2005-06-23 2006-06-16 Cartucho, sistema y procedimiento para diagnósticos médicos automatizados.
US15/387,096 US20170100719A1 (en) 2005-06-23 2016-12-21 Cartridge, system and method for automated medical diagnostics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05105608 2005-06-23
EP05105608.3 2005-06-23

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP11005261.0A Previously-Filed-Application EP2409767B1 (fr) 2005-06-23 2006-06-16 Cartouche modulaire, système et procédé de diagnostic médical automatique
US11/917,955 A-371-Of-International US9568424B2 (en) 2005-06-23 2006-06-16 Cartridge, system and method for automated medical diagnostics
US15/387,096 Continuation US20170100719A1 (en) 2005-06-23 2016-12-21 Cartridge, system and method for automated medical diagnostics

Publications (2)

Publication Number Publication Date
WO2006136990A2 true WO2006136990A2 (fr) 2006-12-28
WO2006136990A3 WO2006136990A3 (fr) 2007-03-08

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Application Number Title Priority Date Filing Date
PCT/IB2006/051941 WO2006136990A2 (fr) 2005-06-23 2006-06-16 Cartouche, systeme et procede pour diagnostic medical automatique

Country Status (8)

Country Link
US (2) US9568424B2 (fr)
EP (2) EP2409767B1 (fr)
JP (1) JP5086250B2 (fr)
CN (2) CN101203312B (fr)
AT (1) ATE534465T1 (fr)
ES (2) ES2689172T3 (fr)
RU (1) RU2432205C2 (fr)
WO (1) WO2006136990A2 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
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WO2007110825A3 (fr) * 2006-03-29 2007-12-13 Koninkl Philips Electronics Nv Systeme de traitement de fluide et de determination de volume
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US20170100719A1 (en) 2017-04-13
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US9568424B2 (en) 2017-02-14
CN101203312A (zh) 2008-06-18
ATE534465T1 (de) 2011-12-15
EP1896180B1 (fr) 2011-11-23
CN101203312B (zh) 2011-08-17
ES2689172T3 (es) 2018-11-08
EP2409767A1 (fr) 2012-01-25
JP5086250B2 (ja) 2012-11-28
US20100047774A1 (en) 2010-02-25
WO2006136990A3 (fr) 2007-03-08
EP2409767B1 (fr) 2018-08-08
CN102268367A (zh) 2011-12-07
RU2008102386A (ru) 2009-07-27
ES2373686T3 (es) 2012-02-07
RU2432205C2 (ru) 2011-10-27

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