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WO2006036735A3 - Method for detecting and quantitating multiple subcellular components - Google Patents

Method for detecting and quantitating multiple subcellular components Download PDF

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Publication number
WO2006036735A3
WO2006036735A3 PCT/US2005/033964 US2005033964W WO2006036735A3 WO 2006036735 A3 WO2006036735 A3 WO 2006036735A3 US 2005033964 W US2005033964 W US 2005033964W WO 2006036735 A3 WO2006036735 A3 WO 2006036735A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
subcellular components
quantitating multiple
quantitating
multiple subcellular
Prior art date
Application number
PCT/US2005/033964
Other languages
French (fr)
Other versions
WO2006036735A2 (en
Inventor
Michael Kilpatrick
Triantafyllos Tafas
Original Assignee
Ikonisys Inc
Michael Kilpatrick
Triantafyllos Tafas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ikonisys Inc, Michael Kilpatrick, Triantafyllos Tafas filed Critical Ikonisys Inc
Priority to EP05798226A priority Critical patent/EP1791979A4/en
Priority to JP2007533625A priority patent/JP2008518587A/en
Priority to AU2005289765A priority patent/AU2005289765A1/en
Priority to CA002580961A priority patent/CA2580961A1/en
Publication of WO2006036735A2 publication Critical patent/WO2006036735A2/en
Publication of WO2006036735A3 publication Critical patent/WO2006036735A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.
PCT/US2005/033964 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components WO2006036735A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP05798226A EP1791979A4 (en) 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components
JP2007533625A JP2008518587A (en) 2004-09-22 2005-09-22 Detection and quantification of subcellular components
AU2005289765A AU2005289765A1 (en) 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components
CA002580961A CA2580961A1 (en) 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61206704P 2004-09-22 2004-09-22
US60/612,067 2004-09-22

Publications (2)

Publication Number Publication Date
WO2006036735A2 WO2006036735A2 (en) 2006-04-06
WO2006036735A3 true WO2006036735A3 (en) 2008-01-24

Family

ID=36119422

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/033964 WO2006036735A2 (en) 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components

Country Status (6)

Country Link
EP (1) EP1791979A4 (en)
JP (1) JP2008518587A (en)
CN (1) CN101228283A (en)
AU (1) AU2005289765A1 (en)
CA (1) CA2580961A1 (en)
WO (1) WO2006036735A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267628A (en) * 2017-07-13 2017-10-20 苏州贝康医疗器械有限公司 Embryonic limb bud cell prochromosome abnormality detection kit

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9864835B2 (en) * 2007-10-15 2018-01-09 23Andme, Inc. Genetic comparisons between grandparents and grandchildren
EP2288903A4 (en) * 2008-05-16 2012-03-21 Biomedical Photometrics Inc Imaging system with dynamic range maximization
AU2010339478B2 (en) * 2009-12-31 2014-08-07 Ventana Medical Systems, Inc. Simultaneous detection of mutational status and gene copy number
CA2859269C (en) * 2012-02-01 2016-12-13 Ventana Medical Systems, Inc. System for detecting genes in tissue samples
JP6231709B1 (en) * 2016-05-31 2017-11-15 シスメックス株式会社 Fluorescence image analyzer and analysis method
JP6959755B2 (en) * 2017-04-14 2021-11-05 シスメックス株式会社 Fluorescence image analyzer, fluorescence image analysis method and computer program
JP6948145B2 (en) 2017-04-14 2021-10-13 シスメックス株式会社 Fluorescent image analyzer, image processing method for fluorescent images and computer programs
CN108956431B (en) * 2018-08-09 2021-04-30 苏州叠代生物科技有限公司 Method for detecting residual cells in platelet product obtained by apheresis
JP7376245B2 (en) 2019-03-29 2023-11-08 シスメックス株式会社 Fluorescence image analysis device and fluorescence image analysis method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136540A (en) * 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US6169816B1 (en) * 1997-05-14 2001-01-02 Applied Imaging, Inc. Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIANCHI ET AL.: "Detection of fetal cells with 47, XY, +21 karyotype in maternal peripheral blood", HUMA. GENET., vol. 90, 1992, pages 368 - 370, XP001069259 *
OERTHER ET AL.: "Simultaneous oligonucleotide probe hybridization and immunostaining for in situ detection of Gordona species in activated sludge", FEMS MICROBIOLOGY ECOLOGY, vol. 29, no. 2, 1999, pages 129 - 136, XP003022483 *
See also references of EP1791979A4 *
WAGGONER ET AL.: "Multiparameter Fluorescence Imaging Microscope: Reagents and Instruments", HUMAN PATHOLOGY, vol. 27, no. 5, May 1996 (1996-05-01), pages 494 - 502, XP000920886 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267628A (en) * 2017-07-13 2017-10-20 苏州贝康医疗器械有限公司 Embryonic limb bud cell prochromosome abnormality detection kit

Also Published As

Publication number Publication date
AU2005289765A1 (en) 2006-04-06
CA2580961A1 (en) 2006-04-06
JP2008518587A (en) 2008-06-05
WO2006036735A2 (en) 2006-04-06
EP1791979A2 (en) 2007-06-06
EP1791979A4 (en) 2009-07-29
CN101228283A (en) 2008-07-23

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