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WO2006034811A3 - Use of laccases as reporter genes - Google Patents

Use of laccases as reporter genes Download PDF

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Publication number
WO2006034811A3
WO2006034811A3 PCT/EP2005/010239 EP2005010239W WO2006034811A3 WO 2006034811 A3 WO2006034811 A3 WO 2006034811A3 EP 2005010239 W EP2005010239 W EP 2005010239W WO 2006034811 A3 WO2006034811 A3 WO 2006034811A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
sequence
laccase
regulatory properties
laccases
Prior art date
Application number
PCT/EP2005/010239
Other languages
German (de)
French (fr)
Other versions
WO2006034811A2 (en
Inventor
Marvin Karos
Thomas Subkowski
Hans-Georg Lemaire
Claus Bollschweiler
Bernhard Nuesslein
Reinhard Fischer
Original Assignee
Basf Ag
Marvin Karos
Thomas Subkowski
Hans-Georg Lemaire
Claus Bollschweiler
Bernhard Nuesslein
Reinhard Fischer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Marvin Karos, Thomas Subkowski, Hans-Georg Lemaire, Claus Bollschweiler, Bernhard Nuesslein, Reinhard Fischer filed Critical Basf Ag
Publication of WO2006034811A2 publication Critical patent/WO2006034811A2/en
Publication of WO2006034811A3 publication Critical patent/WO2006034811A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method for determining the regulatory properties of a genetic element, which comprises the following steps: a) introducing a DNA molecule having a DNA-I sequence whose regulatory properties are to be determined into a cell, said DNA-I sequence being linked with an additional DNA-II sequence encoding a laccase from Aspergillus nidulans in such a manner that the regulatory elements of the DNA-I sequence control expression of the laccase, b) cultivating the cells obtained in step (a) under conditions allowing expression of the laccase, c) associating the conditions selected under (b) with the regulatory properties of the DNA-I sequence.
PCT/EP2005/010239 2004-09-28 2005-09-22 Use of laccases as reporter genes WO2006034811A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004047409.5 2004-09-28
DE200410047409 DE102004047409A1 (en) 2004-09-28 2004-09-28 Use of laccases as reporter genes

Publications (2)

Publication Number Publication Date
WO2006034811A2 WO2006034811A2 (en) 2006-04-06
WO2006034811A3 true WO2006034811A3 (en) 2006-06-22

Family

ID=35457043

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010239 WO2006034811A2 (en) 2004-09-28 2005-09-22 Use of laccases as reporter genes

Country Status (2)

Country Link
DE (1) DE102004047409A1 (en)
WO (1) WO2006034811A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110184244A (en) * 2019-06-20 2019-08-30 福清市火麒麟食用菌技术开发有限公司 A kind of Sparassis crispa produces the preparation method of laccase

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6551797B1 (en) * 1998-04-02 2003-04-22 Consortium für Electrochemische Industrie GmbH Expression system for producing proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6551797B1 (en) * 1998-04-02 2003-04-22 Consortium für Electrochemische Industrie GmbH Expression system for producing proteins

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "23rd Fungal Genetics Conference at Asilomar", INTERNET ARTICLE, 15 March 2005 (2005-03-15), Pacific Grove, California, XP002361523, Retrieved from the Internet <URL:http://www.fgsc.net/asil2005/asil2005.htm> [retrieved on 20051214] *
ANSON D S ET AL: "An improved beta-galactosidase reporter gene.", JOURNAL OF BIOTECHNOLOGY. 19 FEB 2004, vol. 108, no. 1, 19 February 2004 (2004-02-19), pages 17 - 30, XP002359756, ISSN: 0168-1656 *
GOUKA R J ET AL: "Efficient production of secretedproteins by Aspergillus: progress, limitations and prospects", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 47, no. 1, January 1997 (1997-01-01), pages 1 - 11, XP002121584, ISSN: 0175-7598 *
HOEGGER PATRIK J ET AL: "The laccase gene family in Coprinopsis cinerea (Coprinus cinereus).", CURRENT GENETICS. FEB 2004, vol. 45, no. 1, February 2004 (2004-02-01), pages 9 - 18, XP002359751, ISSN: 0172-8083 *
J. K. PEMMASANI ET AL: "Developmental Biology - Poster abstract number 497. Spatial and temporal expression of laccase in Coprinopsis cinerea using galectin promoters.", INTERNET ARTICLE, 2005, pages 1 - 12, XP002359759, Retrieved from the Internet <URL:http://www.fgsc.net/asil2005/DevelopmentalBiology.htm> [retrieved on 20051216] *
JENSEN A B ET AL: "Phosphorylation mediates the nuclear targeting of the maize Rab17 protein.", THE PLANT JOURNAL : FOR CELL AND MOLECULAR BIOLOGY. MAR 1998, vol. 13, no. 5, March 1998 (1998-03-01), pages 691 - 697, XP002359755, ISSN: 0960-7412 *
KAZMIERCZAK P ET AL: "Transcriptional repression of specific host genes by the mycovirus Cryphonectria hypovirus 1.", JOURNAL OF VIROLOGY. FEB 1996, vol. 70, no. 2, February 1996 (1996-02-01), pages 1137 - 1142, XP002359754, ISSN: 0022-538X *
SCHERER M ET AL: "Molecular characterization of a blue-copper laccase, TILA, of Aspergillus nidulans.", FEMS MICROBIOLOGY LETTERS. 30 MAY 2001, vol. 199, no. 2, 30 May 2001 (2001-05-30), pages 207 - 213, XP002359753, ISSN: 0378-1097 *
SCHERER M ET AL: "Purification and characterization of laccase II of Aspergillus nidulans.", ARCHIVES OF MICROBIOLOGY. AUG 1998, vol. 170, no. 2, August 1998 (1998-08-01), pages 78 - 84, XP002359752, ISSN: 0302-8933 *
SPERGEL D J ET AL: "Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies.", PROGRESS IN NEUROBIOLOGY. APR 2001, vol. 63, no. 6, April 2001 (2001-04-01), pages 673 - 686, XP002359757, ISSN: 0301-0082 *
WANG P ET AL: "Identification of a Cryphonectria parasitica laccase gene promoter element involved in cycloheximide-inducible, hypovirus-repressible transcriptional activation", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER, AMSTERDAM, NL, vol. 210, no. 1, 27 March 1998 (1998-03-27), pages 79 - 84, XP004117453, ISSN: 0378-1119 *
WANG S ET AL: "IMPLICATIONS FOR BCD MRNA LOCALIZATION FROM SPATIAL DISTRIBUTION OF EXU PROTEIN IN DROSOPHILA OOGENESIS", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 369, 2 June 1994 (1994-06-02), pages 400 - 403, XP002003600, ISSN: 0028-0836 *
WEENINK XAVIER O ET AL: "A new method for screening and isolation of hypersecretion mutants in Aspergillus niger.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. FEB 2006, vol. 69, no. 6, 14 July 2005 (2005-07-14), pages 711 - 717, XP002369354, ISSN: 0175-7598 *
ZHU XUDONG ET AL: "Copper-mediated reversal of defective laccase in a Deltavph1 avirulent mutant of Cryptococcus neoformans.", MOLECULAR MICROBIOLOGY. FEB 2003, vol. 47, no. 4, February 2003 (2003-02-01), pages 1007 - 1014, XP002359750, ISSN: 0950-382X *

Also Published As

Publication number Publication date
DE102004047409A1 (en) 2006-03-30
WO2006034811A2 (en) 2006-04-06

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