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WO2006031537A3 - Microplate analysis system and method - Google Patents

Microplate analysis system and method Download PDF

Info

Publication number
WO2006031537A3
WO2006031537A3 PCT/US2005/031772 US2005031772W WO2006031537A3 WO 2006031537 A3 WO2006031537 A3 WO 2006031537A3 US 2005031772 W US2005031772 W US 2005031772W WO 2006031537 A3 WO2006031537 A3 WO 2006031537A3
Authority
WO
WIPO (PCT)
Prior art keywords
light
wells
plate
analysis system
illumination
Prior art date
Application number
PCT/US2005/031772
Other languages
French (fr)
Other versions
WO2006031537A2 (en
Inventor
David M Heffelfinger
Robert M Watson Jr
Charles S Smith Iii
Siavash Ghazvini
Christopherm F Bragg
John M Ii Collier
Gibson T Lam
Original Assignee
Alpha Innotech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alpha Innotech Corp filed Critical Alpha Innotech Corp
Publication of WO2006031537A2 publication Critical patent/WO2006031537A2/en
Publication of WO2006031537A3 publication Critical patent/WO2006031537A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G01J1/0488—Optical or mechanical part supplementary adjustable parts with spectral filtering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1213—Filters in general, e.g. dichroic, band
    • G01J2003/1217—Indexed discrete filters or choppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

A method and device for illuminating a multiwell plate, collecting light from a target area with wells of the multiwell plate, transmitting this light to an area array detector, detecting discrete targets within wells of the plate and stitching together images of the well plate. This device and method may include the detection of microarray and non-ordered cells within wells. The system and method may include elements for the homogenization of light, the shaping of illumination light to illuminate a selected area of the array, off axis illumination and spatial modulation of the illumination light.
PCT/US2005/031772 2004-09-09 2005-09-08 Microplate analysis system and method WO2006031537A2 (en)

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
US60844204P 2004-09-09 2004-09-09
US60847004P 2004-09-09 2004-09-09
US60846104P 2004-09-09 2004-09-09
US60843004P 2004-09-09 2004-09-09
US60833004P 2004-09-09 2004-09-09
US60840704P 2004-09-09 2004-09-09
US60845004P 2004-09-09 2004-09-09
US60/608,330 2004-09-09
US60/608,461 2004-09-09
US60/608,407 2004-09-09
US60/608,430 2004-09-09
US60/608,450 2004-09-09
US60/608,470 2004-09-09
US60/608,442 2004-09-09

Publications (2)

Publication Number Publication Date
WO2006031537A2 WO2006031537A2 (en) 2006-03-23
WO2006031537A3 true WO2006031537A3 (en) 2006-08-17

Family

ID=36060532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/031772 WO2006031537A2 (en) 2004-09-09 2005-09-08 Microplate analysis system and method

Country Status (1)

Country Link
WO (1) WO2006031537A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20060495A0 (en) 2006-05-19 2006-05-19 Wallac Oy Arrangement and procedure for illuminating an object
US8244021B2 (en) * 2006-12-20 2012-08-14 Ventana Medical Systems, Inc. Quantitative, multispectral image analysis of tissue specimens stained with quantum dots
US9557217B2 (en) 2007-02-13 2017-01-31 Bti Holdings, Inc. Universal multidetection system for microplates
WO2009081305A1 (en) 2007-12-19 2009-07-02 Koninklijke Philips Electronics N.V. Detection system and method
DE102010016382B4 (en) * 2010-04-09 2022-06-02 Leica Microsystems Cms Gmbh Fluorescence microscope and method for carrying out multi-positioning in a screening application
CH706326A2 (en) 2012-03-14 2013-09-30 Tecan Trading Ag Procedures and microplate readers for study of biological cells or cell cultures.
EP3301431B1 (en) * 2016-09-29 2019-08-28 Roche Diagniostics GmbH Multi-chamber analysis device and method for analyzing
GB2597502A (en) 2020-07-24 2022-02-02 Ffei Ltd A whole slide imaging method for a microscope
EP4320426A4 (en) * 2021-04-07 2025-03-12 Illumina Inc ADJUSTABLE ILLUMINATION PATTERN FOR SAMPLE ANALYSIS
CN114121334B (en) * 2021-11-16 2025-04-22 湖州霍里思特智能科技有限公司 A ray collimation adjustment device
CN118647859A (en) * 2021-12-24 2024-09-13 分子装置有限公司 Microplate Reader

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271042B1 (en) * 1998-08-26 2001-08-07 Alpha Innotech Corporation Biochip detection system
US20010055764A1 (en) * 1999-05-07 2001-12-27 Empedocles Stephen A. Microarray methods utilizing semiconductor nanocrystals
US20020158211A1 (en) * 2001-04-16 2002-10-31 Dakota Technologies, Inc. Multi-dimensional fluorescence apparatus and method for rapid and highly sensitive quantitative analysis of mixtures

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271042B1 (en) * 1998-08-26 2001-08-07 Alpha Innotech Corporation Biochip detection system
US20010055764A1 (en) * 1999-05-07 2001-12-27 Empedocles Stephen A. Microarray methods utilizing semiconductor nanocrystals
US20020158211A1 (en) * 2001-04-16 2002-10-31 Dakota Technologies, Inc. Multi-dimensional fluorescence apparatus and method for rapid and highly sensitive quantitative analysis of mixtures

Also Published As

Publication number Publication date
WO2006031537A2 (en) 2006-03-23

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