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WO2006015374A3 - Compositions et methodes permettant de preparer des acides nucleiques a partir d'echantillons microbiens - Google Patents

Compositions et methodes permettant de preparer des acides nucleiques a partir d'echantillons microbiens Download PDF

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Publication number
WO2006015374A3
WO2006015374A3 PCT/US2005/027715 US2005027715W WO2006015374A3 WO 2006015374 A3 WO2006015374 A3 WO 2006015374A3 US 2005027715 W US2005027715 W US 2005027715W WO 2006015374 A3 WO2006015374 A3 WO 2006015374A3
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WO
WIPO (PCT)
Prior art keywords
methods
compositions
nucleic acids
preparation
microbial samples
Prior art date
Application number
PCT/US2005/027715
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English (en)
Other versions
WO2006015374A2 (fr
Inventor
Yiu-Lian Fong
Azita Tabrizi
Original Assignee
Novartis Vaccines & Diagnostic
Yiu-Lian Fong
Azita Tabrizi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Vaccines & Diagnostic, Yiu-Lian Fong, Azita Tabrizi filed Critical Novartis Vaccines & Diagnostic
Priority to AT05804047T priority Critical patent/ATE527338T1/de
Priority to EP05804047.8A priority patent/EP1774041B9/fr
Priority to US11/659,255 priority patent/US20080193912A1/en
Publication of WO2006015374A2 publication Critical patent/WO2006015374A2/fr
Publication of WO2006015374A3 publication Critical patent/WO2006015374A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes et des compositions permettant de libérer des acides nucléiques à partir d'une variété de types différents de micro-organismes. Ladite méthode repose sur une procédure de lyse simplifiée qui peut être appliquée à de nombreux types de bactéries et de cellules fongiques et facilement automatisée dans des méthodes de criblage à débit élevé. Cette méthode permet d'utiliser une concentration élevée d'agent de chélation et un mélange d'enzymes de lyse afin de soumettre les parois cellulaires microbiennes à une dissociation et permettre la libération de l'acide nucléique.
PCT/US2005/027715 2004-08-03 2005-08-03 Compositions et methodes permettant de preparer des acides nucleiques a partir d'echantillons microbiens WO2006015374A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT05804047T ATE527338T1 (de) 2004-08-03 2005-08-03 Verfahren zur gewinnung von nukleinsäuren durch lyse mikrobieller proben
EP05804047.8A EP1774041B9 (fr) 2004-08-03 2005-08-03 Méthodes permettant d'obtenir des acides nucléiques par lyse d'échantillons microbiens
US11/659,255 US20080193912A1 (en) 2004-08-03 2005-08-03 Compositions and Methods for Preparation of Nucleic Acids from Microbial Samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59863804P 2004-08-03 2004-08-03
US60/598,638 2004-08-03

Publications (2)

Publication Number Publication Date
WO2006015374A2 WO2006015374A2 (fr) 2006-02-09
WO2006015374A3 true WO2006015374A3 (fr) 2007-11-08

Family

ID=35787930

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/027715 WO2006015374A2 (fr) 2004-08-03 2005-08-03 Compositions et methodes permettant de preparer des acides nucleiques a partir d'echantillons microbiens

Country Status (5)

Country Link
US (1) US20080193912A1 (fr)
EP (2) EP2361993A1 (fr)
AT (1) ATE527338T1 (fr)
ES (1) ES2371764T3 (fr)
WO (1) WO2006015374A2 (fr)

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US20120077206A1 (en) 2003-07-12 2012-03-29 Accelr8 Technology Corporation Rapid Microbial Detection and Antimicrobial Susceptibility Testing
US7687239B2 (en) 2003-07-12 2010-03-30 Accelrs Technology Corporation Sensitive and rapid determination of antimicrobial susceptibility
EP2255010B1 (fr) 2008-02-20 2018-05-30 Streck Inc. Thermocycleur et récipient à échantillons pour l'amplification rapide de l'adn
WO2009114520A2 (fr) * 2008-03-10 2009-09-17 Pharmain Corporation Compositions de traitement ayant des métallopeptidases, procédés de fabrication et utilisation de celles-ci
CA2761943A1 (fr) * 2009-05-14 2010-11-18 Streck, Inc. Cassette de traitement d'echantillons, systeme et procede associes
WO2012050787A1 (fr) * 2010-09-29 2012-04-19 Ibis Biosciences, Inc. Extraction d'acide nucléique fongique
US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
ES2551922T3 (es) 2011-03-07 2015-11-24 Accelerate Diagnostics, Inc. Sistemas rápidos de purificación celular
WO2012166913A1 (fr) 2011-06-01 2012-12-06 Streck, Inc. Système thermocycleur rapide pour une amplification rapide d'acides nucléiques et procédés s'y rapportant
CA2879638A1 (fr) 2012-08-10 2014-02-13 Streck, Inc. Systeme optique en temps reel pour reaction en chaine par polymerase
US20150225762A1 (en) * 2012-09-10 2015-08-13 Accelerate Diagnostics, Inc. Same-day blood culture with digital microscopy
US9677109B2 (en) 2013-03-15 2017-06-13 Accelerate Diagnostics, Inc. Rapid determination of microbial growth and antimicrobial susceptibility
EP3014251A1 (fr) 2013-06-28 2016-05-04 Streck Inc. Dispositifs pour réaction en chaîne de polymérase en temps réel
US10253355B2 (en) 2015-03-30 2019-04-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
AU2016243656A1 (en) 2015-03-30 2017-11-09 Accelerate Diagnostics, Inc. Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing
FR3035409B1 (fr) * 2015-04-21 2020-01-24 Fondation Mediterranee Infection Methode d'extraction d'adn comprenant un traitement de degradation enzymatique d'un echantillon biologique
US10888508B1 (en) * 2018-06-21 2021-01-12 Saied Mostafa Moazzami Multi-functional coolant for dentistry use
CN109402238A (zh) * 2018-12-19 2019-03-01 广州海思医疗科技有限公司 用于血液、口腔拭子进行pcr的裂解液及试剂盒
CN109486903A (zh) * 2018-12-25 2019-03-19 广州海思医疗科技有限公司 一种用于将拭子上的细胞洗脱、裂解释放核酸的洗脱裂解液、试剂盒和方法
CN110283816A (zh) * 2019-07-08 2019-09-27 无锡市第二人民医院 一种磁珠法微生物基因组dna的提取试剂盒及提取方法
CN110804611A (zh) * 2019-11-13 2020-02-18 北京贝尔生物工程股份有限公司 一种适用于细菌和/或真菌的基因组dna提取方法
CN112391384B (zh) * 2021-01-11 2023-03-10 甘肃省科学院传感技术研究所 一种快速提取革兰氏阴性菌基因组dna的试剂盒及提取方法
CN116694472A (zh) * 2023-07-18 2023-09-05 墨卓生物科技(浙江)有限公司 一种微生物裂解液、裂解方法及试剂盒

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US20040072241A1 (en) * 2002-01-08 2004-04-15 Biosite Diagnostics, A Delaware Corporation Methods for detecting B. anthracis infection

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US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
DE4236708A1 (de) * 1992-10-30 1994-05-05 Bayer Ag Spezifische Gensonden und Verfahren zur Diagnostik von Candida albicans
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
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Also Published As

Publication number Publication date
US20080193912A1 (en) 2008-08-14
EP1774041B9 (fr) 2013-04-17
EP1774041A4 (fr) 2009-01-21
ES2371764T3 (es) 2012-01-09
EP1774041B1 (fr) 2011-10-05
WO2006015374A2 (fr) 2006-02-09
ATE527338T1 (de) 2011-10-15
EP2361993A1 (fr) 2011-08-31
EP1774041A2 (fr) 2007-04-18

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