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WO2006086669A3 - Aptamer regulated nucleic acids and uses thereof - Google Patents

Aptamer regulated nucleic acids and uses thereof Download PDF

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Publication number
WO2006086669A3
WO2006086669A3 PCT/US2006/004801 US2006004801W WO2006086669A3 WO 2006086669 A3 WO2006086669 A3 WO 2006086669A3 US 2006004801 W US2006004801 W US 2006004801W WO 2006086669 A3 WO2006086669 A3 WO 2006086669A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
aptamer
regulated nucleic
ligand
aptamer regulated
Prior art date
Application number
PCT/US2006/004801
Other languages
French (fr)
Other versions
WO2006086669A2 (en
WO2006086669A8 (en
Inventor
Christina D Smolke
Travis S Bayer
Original Assignee
California Inst Of Techn
Christina D Smolke
Travis S Bayer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2005/036161 external-priority patent/WO2006042112A2/en
Application filed by California Inst Of Techn, Christina D Smolke, Travis S Bayer filed Critical California Inst Of Techn
Priority to US11/884,110 priority Critical patent/US8772464B2/en
Publication of WO2006086669A2 publication Critical patent/WO2006086669A2/en
Publication of WO2006086669A8 publication Critical patent/WO2006086669A8/en
Publication of WO2006086669A3 publication Critical patent/WO2006086669A3/en
Priority to US14/287,824 priority patent/US9309568B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to aptamer-regulated, ligand-responsive nucleic acids, or 'ampliSwitches,' and uses thereof. Particular embodiments include a ligand- responsive nucleic acid that comprises a primer sequence domain and an aptamer domain that is responsive to a ligand.
PCT/US2006/004801 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof WO2006086669A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/884,110 US8772464B2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof
US14/287,824 US9309568B2 (en) 2004-10-05 2014-05-27 Aptamer regulated nucleic acids and uses thereof

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US65142405P 2005-02-09 2005-02-09
US60/651,424 2005-02-09
PCT/US2005/036161 WO2006042112A2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof
USPCT/US2005/036161 2005-10-05
US11/243,889 2005-10-05
US11/243,889 US9315862B2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/036161 Continuation-In-Part WO2006042112A2 (en) 2004-10-05 2005-10-05 Aptamer regulated nucleic acids and uses thereof

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/884,110 A-371-Of-International US8772464B2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof
US14/287,824 Division US9309568B2 (en) 2004-10-05 2014-05-27 Aptamer regulated nucleic acids and uses thereof

Publications (3)

Publication Number Publication Date
WO2006086669A2 WO2006086669A2 (en) 2006-08-17
WO2006086669A8 WO2006086669A8 (en) 2006-12-07
WO2006086669A3 true WO2006086669A3 (en) 2007-03-01

Family

ID=36580287

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/004801 WO2006086669A2 (en) 2004-10-05 2006-02-09 Aptamer regulated nucleic acids and uses thereof

Country Status (1)

Country Link
WO (1) WO2006086669A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9315862B2 (en) 2004-10-05 2016-04-19 California Institute Of Technology Aptamer regulated nucleic acids and uses thereof

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008058291A2 (en) 2006-11-09 2008-05-15 California Institute Of Technology Modular aptamer-regulated ribozymes
US20090082217A1 (en) * 2007-07-16 2009-03-26 California Institute Of Technology Selection of nucleic acid-based sensor domains within nucleic acid switch platform
US20120165387A1 (en) 2007-08-28 2012-06-28 Smolke Christina D General composition framework for ligand-controlled RNA regulatory systems
US8865667B2 (en) 2007-09-12 2014-10-21 California Institute Of Technology Higher-order cellular information processing devices
US9029524B2 (en) 2007-12-10 2015-05-12 California Institute Of Technology Signal activated RNA interference
US9145555B2 (en) 2009-04-02 2015-09-29 California Institute Of Technology Integrated—ligand-responsive microRNAs
US9310357B2 (en) * 2012-09-28 2016-04-12 Src, Inc. Detection of chemical and biological agents using oligonucleotide aptamers
JP6267217B2 (en) 2013-09-30 2018-01-24 Ｎｅｃソリューションイノベータ株式会社 Target analysis method and analysis kit
EP3464596A1 (en) * 2016-06-07 2019-04-10 Cepheid Thermostable polymerase inhibitor compositions and methods
US11359240B2 (en) 2017-03-15 2022-06-14 National University Corporation Gunma University Method for detecting target molecule in which rolling circle amplification is used
TW201930593A (en) * 2017-09-11 2019-08-01 英商卡美納生物科學公司 Compositions and methods for complex unbiased solution profiling
GB2570157A (en) * 2018-01-15 2019-07-17 Dnanudge Ltd Molecule detection
US10467679B1 (en) 2019-04-15 2019-11-05 Dnanudge Limited Product recommendation device and method
US10811140B2 (en) 2019-03-19 2020-10-20 Dnanudge Limited Secure set-up of genetic related user account
US10699806B1 (en) 2019-04-15 2020-06-30 Dnanudge Limited Monitoring system, wearable monitoring device and method
CN114990126B (en) * 2022-08-03 2022-11-18 珠海宝锐生物科技有限公司 Aptamer specifically binding to Bst DNA polymerase large-fragment active site and application thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FAMULOK M.: "Bringing picomolar protein detection into proximity", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 20, May 2002 (2002-05-01), pages 448 - 449, XP002375041, ISSN: 1087-0156 *
FREDRIKSSON S. ET AL.: "Protein detection using proximity-dependent DNA ligation assays", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 20, May 2002 (2002-05-01), pages 473 - 477, XP002375040, ISSN: 1087-0156 *
NUTIU R. ET AL.: "Structure-switching signaling aptamers: transducing molecular recognition into fluorescence signaling", CHEMISTRY - A EUROPEAN JOURNAL, VCH PUBLISHERS, US, vol. 10, no. 8, 19 April 2004 (2004-04-19), pages 1868 - 1876, XP002374936, ISSN: 0947-6539 *
SILVERMAN S.K.: "Rube Goldberg goes (ribo)nuclear ? Molecular switches and sensors made from RNA", RNA, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 9, no. 4, April 2003 (2003-04-01), pages 377 - 383, XP002374942, ISSN: 1355-8382 *
WINKLER W.C. ET AL.: "An mRNA structure that controls gene expression by binding FMN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 99, no. 25, 10 December 2002 (2002-12-10), pages 15908 - 15913, XP002374934, ISSN: 0027-8424 *
WINKLER W.C. ET AL.: "Genetic control by metabolite-binding riboswitches", CHEMBIOCHEM - A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY, WILEY VCH, WEINHEIM, DE, vol. 4, 2003, pages 1024 - 1032, XP002374935, ISSN: 1439-4227 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9315862B2 (en) 2004-10-05 2016-04-19 California Institute Of Technology Aptamer regulated nucleic acids and uses thereof

Also Published As

Publication number Publication date
WO2006086669A2 (en) 2006-08-17
WO2006086669A8 (en) 2006-12-07

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