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WO2006067322A3 - Procede in vitro de production d’ovocytes ou d'œufs presentant une modification genomique ciblee - Google Patents

Procede in vitro de production d’ovocytes ou d'œufs presentant une modification genomique ciblee Download PDF

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Publication number
WO2006067322A3
WO2006067322A3 PCT/FR2005/003182 FR2005003182W WO2006067322A3 WO 2006067322 A3 WO2006067322 A3 WO 2006067322A3 FR 2005003182 W FR2005003182 W FR 2005003182W WO 2006067322 A3 WO2006067322 A3 WO 2006067322A3
Authority
WO
WIPO (PCT)
Prior art keywords
eggs
targeted genomic
vitro method
genomic modification
producing oocytes
Prior art date
Application number
PCT/FR2005/003182
Other languages
English (en)
Other versions
WO2006067322A2 (fr
Inventor
Jean-Stephane Joly
Violette Thermes
Frederic Sohm
Original Assignee
Agronomique Inst Nat Rech
Jean-Stephane Joly
Violette Thermes
Frederic Sohm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Jean-Stephane Joly, Violette Thermes, Frederic Sohm filed Critical Agronomique Inst Nat Rech
Priority to JP2007546126A priority Critical patent/JP2008523796A/ja
Priority to CA002591509A priority patent/CA2591509A1/fr
Priority to EP05850535A priority patent/EP1824984A2/fr
Publication of WO2006067322A2 publication Critical patent/WO2006067322A2/fr
Publication of WO2006067322A3 publication Critical patent/WO2006067322A3/fr
Priority to US11/818,517 priority patent/US20080113437A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de production d'ovocytes ou d'œufs de vertébrés non humains possédant une modification génomigue ciblée ainsi qu'un procédé d'intégration aléatoire d'une séquence d'acides nucléiques exogène dans l'ADN génomique d'une cellule hôte.
PCT/FR2005/003182 2004-12-17 2005-12-19 Procede in vitro de production d’ovocytes ou d'œufs presentant une modification genomique ciblee WO2006067322A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2007546126A JP2008523796A (ja) 2004-12-17 2005-12-19 標的ゲノム改変を有する卵母細胞または卵細胞のインビトロでの作成方法
CA002591509A CA2591509A1 (fr) 2004-12-17 2005-12-19 Procede in vitro de production d'ovocytes ou d'oeufs presentant une modification genomique ciblee
EP05850535A EP1824984A2 (fr) 2004-12-17 2005-12-19 Procede in vitro de production d'ovocytes ou d'oeufs presentant une modification genomique ciblee
US11/818,517 US20080113437A1 (en) 2004-12-17 2007-06-14 In-vitro method for producing oocytes or eggs having targeted genomic modification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0413521A FR2879622B1 (fr) 2004-12-17 2004-12-17 Procede in vitro de production d'ovocytes ou d'oeufs presentant une modification genomique ciblee
FR0413521 2004-12-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/818,517 Continuation US20080113437A1 (en) 2004-12-17 2007-06-14 In-vitro method for producing oocytes or eggs having targeted genomic modification

Publications (2)

Publication Number Publication Date
WO2006067322A2 WO2006067322A2 (fr) 2006-06-29
WO2006067322A3 true WO2006067322A3 (fr) 2007-04-05

Family

ID=34954272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2005/003182 WO2006067322A2 (fr) 2004-12-17 2005-12-19 Procede in vitro de production d’ovocytes ou d'œufs presentant une modification genomique ciblee

Country Status (8)

Country Link
US (1) US20080113437A1 (fr)
EP (1) EP1824984A2 (fr)
JP (1) JP2008523796A (fr)
KR (1) KR20070102517A (fr)
CN (1) CN101175858A (fr)
CA (1) CA2591509A1 (fr)
FR (1) FR2879622B1 (fr)
WO (1) WO2006067322A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103333910A (zh) * 2013-06-09 2013-10-02 北京市水产科学研究所 制备鲟鱼卵母细胞成熟相关蛋白的方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109536526B (zh) 2012-04-25 2020-06-09 瑞泽恩制药公司 核酸酶介导的使用大靶向载体的靶向
CA2908697C (fr) 2013-04-16 2023-12-12 Regeneron Pharmaceuticals, Inc. Modification ciblee du genome d'un rat
WO2015066205A1 (fr) * 2013-10-29 2015-05-07 Rutgers, The State University Of New Jersey Organisme à modification génétique rapide, systèmes d'édition précise du génome et manipulation génétique ciblée par recombinaison homologue améliorée
SG10201700961TA (en) 2013-12-11 2017-04-27 Regeneron Pharma Methods and compositions for the targeted modification of a genome
DK3152312T3 (da) 2014-06-06 2020-04-06 Regeneron Pharma Fremgangsmåder og sammensætninger til modifikation af et mållocus
RU2771532C2 (ru) 2014-06-26 2022-05-05 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленных генетических модификаций и способы их применения
SG11201702309RA (en) 2014-10-15 2017-04-27 Regeneron Pharma Methods and compositions for generating or maintaining pluripotent cells
SG10201913829YA (en) 2014-11-21 2020-03-30 Regeneron Pharma METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAs
NZ732895A (en) 2014-12-19 2022-05-27 Regeneron Pharma Methods and compositions for targeted genetic modification through single-step multiple targeting
JP7004651B2 (ja) * 2016-07-19 2022-02-04 株式会社バイオダイナミクス研究所 長鎖一本鎖ｄｎａを調製する方法
WO2019113743A1 (fr) * 2017-12-11 2019-06-20 清华大学 Procédé de modification génétique

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025183A2 (fr) * 2001-09-14 2003-03-27 Cellectis Integration aleatoire d'un polynucleotide par linearisation in vivo
US6566579B1 (en) * 1996-04-18 2003-05-20 Institut Pasteur I-Sce I induced gene replacement and gene conversion in embryonic stem cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5474896A (en) * 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566579B1 (en) * 1996-04-18 2003-05-20 Institut Pasteur I-Sce I induced gene replacement and gene conversion in embryonic stem cells
WO2003025183A2 (fr) * 2001-09-14 2003-03-27 Cellectis Integration aleatoire d'un polynucleotide par linearisation in vivo

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOULIKA A ET AL: "INDUCTION OF HOMOLOGOUS RECOMBINATION IN MAMMALIAN CHROMOSOMES BY USING THE I-SCEI SYSTEM OF SACCHAROMYCES CEREVISIAE", MOLECULAR AND CELLULAR BIOLOGY, WASHINGTON, DC, US, vol. 15, no. 4, 1 April 1995 (1995-04-01), pages 1968 - 1973, XP000572017, ISSN: 0270-7306 *
SEGAL D J ET AL: "Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 92, no. 3, 31 January 1995 (1995-01-31), pages 806 - 810, XP002982189, ISSN: 0027-8424 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103333910A (zh) * 2013-06-09 2013-10-02 北京市水产科学研究所 制备鲟鱼卵母细胞成熟相关蛋白的方法
CN103333910B (zh) * 2013-06-09 2014-08-20 北京市水产科学研究所 制备鲟鱼卵母细胞成熟相关蛋白的方法

Also Published As

Publication number Publication date
FR2879622B1 (fr) 2008-02-01
CA2591509A1 (fr) 2006-06-29
US20080113437A1 (en) 2008-05-15
FR2879622A1 (fr) 2006-06-23
JP2008523796A (ja) 2008-07-10
KR20070102517A (ko) 2007-10-18
WO2006067322A2 (fr) 2006-06-29
CN101175858A (zh) 2008-05-07
EP1824984A2 (fr) 2007-08-29

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