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WO2006066001A3 - Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes - Google Patents

Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes Download PDF

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Publication number
WO2006066001A3
WO2006066001A3 PCT/US2005/045429 US2005045429W WO2006066001A3 WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3 US 2005045429 W US2005045429 W US 2005045429W WO 2006066001 A3 WO2006066001 A3 WO 2006066001A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
immobilized
culture device
eukaryotic cells
cationic polymer
Prior art date
Application number
PCT/US2005/045429
Other languages
English (en)
Other versions
WO2006066001A2 (fr
Inventor
Yasunobu Tanaka
Chris P Castello
Lei Yu
Original Assignee
Nitto Denko Corp
Yasunobu Tanaka
Chris P Castello
Lei Yu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Denko Corp, Yasunobu Tanaka, Chris P Castello, Lei Yu filed Critical Nitto Denko Corp
Priority to CA002590820A priority Critical patent/CA2590820A1/fr
Priority to KR1020137000063A priority patent/KR20130010036A/ko
Priority to JP2007546895A priority patent/JP4739352B2/ja
Priority to EP05854197A priority patent/EP1836293A2/fr
Priority to AU2005316501A priority patent/AU2005316501B2/en
Publication of WO2006066001A2 publication Critical patent/WO2006066001A2/fr
Publication of WO2006066001A3 publication Critical patent/WO2006066001A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un dispositif de transfection/culture cellulaire qui comprend un support solide recouvert d'un cation polymère dégradable en tant que réactif de transfection. Ce dispositif de transfection/culture cellulaire est stocké de manière pratique à température ambiante jusqu'à son utilisation. Pour effectuer facilement la transfection cellulaire, l'acide nucléique d'intérêt et les cellules à transfecter sont ajoutés au dispositif de transfection/culture. La transfection cellulaire est achevée en moins d'une heure au moyen du dispositif de transfection/culture selon l'invention.
PCT/US2005/045429 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes WO2006066001A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002590820A CA2590820A1 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes
KR1020137000063A KR20130010036A (ko) 2004-12-17 2005-12-14 진핵 세포의 트랜스펙션을 위한 고정화된 분해가능한 양이온성 중합체
JP2007546895A JP4739352B2 (ja) 2004-12-17 2005-12-14 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面
EP05854197A EP1836293A2 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes
AU2005316501A AU2005316501B2 (en) 2004-12-17 2005-12-14 Immobilized degradable cationic polymer for transfecting eukaryotic cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17

Publications (2)

Publication Number Publication Date
WO2006066001A2 WO2006066001A2 (fr) 2006-06-22
WO2006066001A3 true WO2006066001A3 (fr) 2006-09-08

Family

ID=36353906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/045429 WO2006066001A2 (fr) 2004-12-17 2005-12-14 Surface solide comprenant un polymere cationique degradable immobilise afin de transfecter des cellules eucaryotes

Country Status (7)

Country Link
EP (1) EP1836293A2 (fr)
JP (1) JP4739352B2 (fr)
KR (2) KR101263173B1 (fr)
CN (1) CN101124316A (fr)
AU (1) AU2005316501B2 (fr)
CA (1) CA2590820A1 (fr)
WO (1) WO2006066001A2 (fr)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing

Families Citing this family (23)

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US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2407243B1 (fr) 2003-07-31 2020-04-22 Handylab, Inc. Dispositif multicouche microfluidique
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP3088083B1 (fr) 2006-03-24 2018-08-01 Handylab, Inc. Méthode pour la mise en oeuvre de pcr au moyen d'une cartouche avec plusieures pistes
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
RU2690374C2 (ru) 2011-04-15 2019-06-03 Бектон, Дикинсон Энд Компани Сканирующий в режиме реального времени микрожидкостный термоциклер и способы синхронизированных термоциклирования и сканирующего оптического обнаружения
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
CN102827756B (zh) * 2012-06-25 2014-04-09 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
DK3013964T3 (da) * 2013-06-28 2020-06-15 Ethris Gmbh Sammensætninger til indføring af rna i celler
BR112017009505A2 (pt) * 2014-11-10 2018-02-06 Ethris Gmbh composição farmacêutica, e, matriz ou suporte.
CA3036604A1 (fr) * 2016-10-05 2018-04-12 Syngenta Participations Ag Perfectionnements apportes ou se rapportant a l'extinction genique
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
EP3798250A1 (fr) * 2019-09-25 2021-03-31 University College Dublin Polymères cationiques hyperramifiés utiles en tant que vecteurs d'administration d'acide nucléique pour la transfection
CN112195193A (zh) * 2020-09-30 2021-01-08 上海交通大学 单链dna在基因转染中的应用
CN114904003B (zh) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 可电离的阳离子脂质类似物材料在作为核酸药物递送载体或转染试剂中的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (fr) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Procedes
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

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EP1218529B1 (fr) * 1999-09-17 2006-11-22 Whitehead Institute For Biomedical Research Procede de transfection inverse
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
DE60126115T2 (de) * 2000-11-03 2007-08-23 The Regents Of The University Of Michigan, Ann Arbor Oberflächentransfektion und expressionsverfahren
EP1513538A4 (fr) * 2002-06-14 2007-08-22 Mirus Bio Corp Nouveaux procedes d'apport de polynucleotides dans des cellules
US6878374B2 (en) * 2003-02-25 2005-04-12 Nitto Denko Corporation Biodegradable polyacetals
WO2005083091A1 (fr) * 2004-02-18 2005-09-09 Nitto Denko Corporation Dispositif de culture et procede de transfection de cellule eucaryote

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
EP1398042A1 (fr) * 2002-09-10 2004-03-17 Lipotek, Inc Transfection d' acides nucleiques
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
WO2005023312A2 (fr) * 2003-09-03 2005-03-17 Imperial College Innovations Limited Procedes
US20050176132A1 (en) * 2004-02-10 2005-08-11 Yasunobu Tanaka Culture device and method for eukaryotic cell transfection

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US9458487B2 (en) 2002-10-08 2016-10-04 Pfenex, Inc. Expression of mammalian proteins in pseudomonas fluorescens
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US9109229B2 (en) 2004-07-26 2015-08-18 Pfenex Inc. Process for improved protein expression by strain engineering
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder

Also Published As

Publication number Publication date
KR20130010036A (ko) 2013-01-24
KR20070101859A (ko) 2007-10-17
AU2005316501B2 (en) 2010-10-21
EP1836293A2 (fr) 2007-09-26
WO2006066001A2 (fr) 2006-06-22
JP2008523810A (ja) 2008-07-10
AU2005316501A1 (en) 2006-06-22
KR101263173B1 (ko) 2013-05-13
JP4739352B2 (ja) 2011-08-03
CN101124316A (zh) 2008-02-13
CA2590820A1 (fr) 2006-06-22

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