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WO2005112665A1 - Composition containing processed sweet potato foliage - Google Patents

Composition containing processed sweet potato foliage Download PDF

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Publication number
WO2005112665A1
WO2005112665A1 PCT/JP2005/009508 JP2005009508W WO2005112665A1 WO 2005112665 A1 WO2005112665 A1 WO 2005112665A1 JP 2005009508 W JP2005009508 W JP 2005009508W WO 2005112665 A1 WO2005112665 A1 WO 2005112665A1
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WO
WIPO (PCT)
Prior art keywords
sweet potato
leaves
stems
powder
composition
Prior art date
Application number
PCT/JP2005/009508
Other languages
French (fr)
Japanese (ja)
Inventor
Kinya Takagaki
Original Assignee
Toyo Shinyaku Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Shinyaku Co., Ltd. filed Critical Toyo Shinyaku Co., Ltd.
Priority to JP2006513766A priority Critical patent/JPWO2005112665A1/en
Publication of WO2005112665A1 publication Critical patent/WO2005112665A1/en

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/105Sweet potatoes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics

Definitions

  • the present invention relates to a composition containing a processed product of a sweet potato stem and leaves, and more particularly, a composition containing a young shoot of a sweet potato (particularly, a site 60 cm or less from the tip of the sweet potato leaf).
  • Sweet potatoes belonging to the convolvulaceae family are hardly eaten in modern food-rich days, and their effective use is required.
  • Japanese Unexamined Patent Publication (Kokai) No. 7-111878 describes a foodstuff containing sweet potato obtained by immersing the stems and leaves of a sweet potato in water, boiling it, concentrating the eluate, and applying the obtained extract to nori. Have been.
  • Japanese Patent Application Laid-Open No. 6-627883 discloses a method for treating sweet potato stems and leaves by washing with spouted water so as to prevent effective ingredients such as vitamins and minerals contained therein from flowing out when washing the sweet potato stems and leaves. It is described that drying, drying and powdering are performed.
  • the sweet potato stems and leaves contain various excellent components, such as polyphenol, in addition to the components noted in the above literature. Therefore, it is still insufficient in terms of effective use of sweet potato stems and leaves.
  • the stem of sweet potato stems and leaves are hard fibrous materials, it is difficult to formulate such as powder, and as a result, the applicable range is limited to a part of foods or pharmaceuticals. There are also problems such as the instability of the components contained in the sweet potato stems and leaves. Disclosure of the invention
  • An object of the present invention is to provide a processed product of sweet potato leaves which is easy to process and has a high active ingredient content, particularly a high polyphenol content.
  • the inventors of the present application have conducted intensive studies on a processed product of sweet potato stems and leaves having a high active ingredient content, particularly a high polyphenol content. As a result, it was found that by using the young shoots of sweet potato stems and leaves, processing was easy and a processed product of sweet potato stems and leaves rich in active ingredients was obtained. Furthermore, it was found that by combining a processed product of sweet potato foliage with a sugar alcohol or a disaccharide or higher sugar, excellent granulation properties, storage stability, palatability, and diabetes prevention effects were obtained. The invention has been completed.
  • composition of the present invention contains a processed product of sweet potato stems and leaves, and the young shoots are contained in the sweet potato stems and leaves.
  • the sweet potato leaves are at least a part of the above-ground portions of sweet potato leaves having a length of 300 cm or less.
  • the young stem leaf of the sweet potato is a site of 60 cm or less from the tip of the sweet potato leaf.
  • composition of the present invention also contains a processed product of sweet potato foliage and at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or higher.
  • young shoots are contained in the sweet potato shoots.
  • the saccharide is an indigestible dextrin.
  • the composition of the present invention contains a processed product of sweet potato stems and leaves, and the sweet potato stems and leaves include young shoots (hereinafter, referred to as a first composition). Alternatively, at least one selected from the group consisting of a processed product of sweet potato stem and leaves and a sugar alcohol and a sugar of two or more sugars
  • the sweet potatoes preferably contain young shoots (hereinafter, referred to as a second itotsuto product).
  • a second itotsuto product hereinafter, first, the sweet potato stem and leaves used in the first composition and the second thread and the processed product thereof will be described.
  • the variety of the sweet potato is not particularly limited.
  • the foliage of "Suiou” which is a special variety is preferably used.
  • the foliage of "Suiou” has a high polyphenol content, and is characterized in that foliage is regenerated again even after the foliage is once harvested.
  • Suio is rich in polyphenols even in regenerated foliage. For these reasons, it is particularly preferred in the present invention to use sweet potato foliage.
  • sweet potato stem and leaves refers to "at least a part of the stem and leaves of sweet potato".
  • the stalk of the sweet potato may include any of the above-ground stalk and the underground stalk (underground stalk), but the above-ground stalk is preferably used.
  • the shoots and leaves of the aerial part of the sweet potato that is, the stems and leaves of the aerial part that are produced during sweet potato cultivation are preferably used.
  • the size of the foliage of the sweet potato used in the present invention is not particularly limited.
  • the sweet potato stems and leaves grow particularly large when growing, such grown sweet potato stems and leaves are preferable in terms of a large yield of processed products obtained.
  • processing may be difficult, or the resulting processed product may have a reduced nutritional value, especially the polyphenol content. Therefore, it is usually preferable to use sweet potato stems and leaves of a predetermined size.
  • the length of the above-ground portion that is, the length from the position where the sweet potato stems are out of the ground (ground surface) to the tip of the sweet potato leaf, is preferably 10 cm or more, and more preferably 30 cm.
  • more preferably 40 cm or more most preferably 60 cm or more, preferably 300 cm or less, more preferably 200 cm or less, even more preferably 150 cm or less, is used. If the length of the aerial part exceeds 300 cm, the tip of the sweet potato stems and leaves will easily touch the ground, and it will be susceptible to pests and other insects, and if so, it may not be possible to harvest a sufficient amount of sweet potato stems and leaves There is.
  • sweet potato stems and leaves it is preferable to use sweet potato stems and leaves further retaining green.
  • Such sweet potato stems and leaves are easy to process because the plant itself is soft, and when processed directly into dry powder etc., a dry powder with a pleasant texture can be obtained. .
  • the sweet potato foliage used in the composition of the present invention may include a young foliage.
  • the young shoots are essential for the first composition.
  • the term "young shoots" of the sweet potato refers to a site at the tip of the sweet potato stem and leaves, which has a yellowish green color compared to other stems and leaves.
  • the site is preferably 60 cm or less, more preferably 40 cm or less, even more preferably 20 cm or less, and most preferably 10 cm or less from the most advanced stem or leaf. Since the young shoots of sweet potatoes contain a large amount of polyphenol, they are particularly preferably used among sweet potato shoots.
  • the sweet potato foliage used in the present invention contains the young foliage preferably in an amount of 0.1% by mass or more, more preferably 1% by mass or more in terms of dry mass.
  • the stem and leaves of the sweet potato used in the present invention further contain a large amount of dicaffeoylquinic acid and tricaffeoylquinic acid (hereinafter referred to as dicaffeoylquinic acid and the like).
  • Sweet potato stems and leaves (especially sugarcane stems and leaves) generally contain more tricaffeoylquinic acid than other plants.
  • sweet potato foliage containing 2 mg to 300 mg, preferably 10 mg to 30 Omg of tricaffeoylquinic acid per 100 g of dry mass is suitably used.
  • sweet potato stems and leaves containing 100 mg to 600 mg of dicaffeoylquinic acid are preferred. This
  • the sweet potato foliage containing such dicaffeoylquinic acid is preferably a foliage foliage, particularly preferably a foliage young foliage.
  • the stem and leaves of the sweet potato used in the present invention are usually subjected to processing after washing mud and the like with water.
  • the processed product of the sweet potato stem and leaves used in the present invention is not particularly limited as long as the sweet potato stem and leaves or the washed product thereof is treated.
  • Such a processed product of sweet potato stems and leaves is obtained by treating sweet potato stems and leaves with, for example, at least one selected from the group consisting of crushing, heat treatment, solid-liquid separation, extraction, drying, and powdering. .
  • Specific examples include crushed sweet potato stems and leaves, heat-treated products, squeezed juices, extracts, extracts, dried products, dried powders, and dried extract powders.
  • the above-mentioned treatment methods crushing, heat treatment, solid-liquid separation treatment, extraction, drying, and grinding treatment
  • the crushing is performed using a crushing means commonly used by those skilled in the art, such as a food processor and a masco mouth whatsoever. From the viewpoint of facilitating crushing, it is preferable to use a food processor or the like to coarsely crush and then further crush using a Masco mouth ider. Due to crushing, for example, the major axis is 0.1 mn! Fragments of sweet potato stems and leaves of about 20 mm can be obtained.
  • the heat treatment is performed to deactivate enzymes in the sweet potato stems and leaves and stabilize the quality, or to prevent fading of the sweet potato stems and leaves.
  • the heat treatment include a plating treatment (blanching), a dry heat treatment, a microwave treatment, an infrared or far-infrared treatment, and a steam treatment.
  • it is a planting process. This planting process is performed to prevent the color of chlorophyll from fading.
  • a method usually performed by a trader, particularly desalination, is suitably employed.
  • the heat treatment can be appropriately performed on sweet potato stems and leaves and processed products thereof (eg, crushed material, squeezed juice, dried powder, dried extract powder, etc.). When the sweet potato stems and leaves are heat-treated as it is, it is preferable to cut and use the major diameter of about 1 mm to 30 cm as necessary for convenience of the treatment step.
  • the conditions of the heat treatment vary greatly depending on the type of sweet potato used, and furthermore, problems such as impairment of flavor and nutrients, and susceptibility to inactivation of useful components of physiological activity are likely to occur.
  • the heat treatment is performed at pH 5.4 or more, preferably pH 5.6 or more and less than 8.4, more preferably 5.6 to 8.0, and most preferably 5.6 to 7.6. Do.
  • an active ingredient for example, polyphenol such as tricaffeoylquinic acid or dicaffeoylquinic acid
  • the hot water having the above pH it is preferable to use the hot water having the above pH from the viewpoint of obtaining a processed product of sweet potato foliage having a high active ingredient.
  • microwave treatment, infrared or far-infrared treatment, or steam treatment perform treatment such as spraying a solution adjusted to the above-mentioned pH to foliage in advance, and then heat treatment. do it.
  • Adjustment of pH can be performed by a method commonly used by those skilled in the art. For example, when adjusting to basic, sodium hydroxide, baking soda, calcium carbonate (eg, eggshell calcium, scallop shell calcium, coral calcium, etc.) as a pH adjuster, and calcined calcium carbonate are obtained. Calcium oxide or the like may be used. Further, as the aqueous solution adjusted to a predetermined pH, alkali ion water or the like may be used. When adjusted to be acidic, organic acids such as acetic acid, cunic acid, ascorbic acid, tartaric acid, malic acid, and fumaric acid can be used. The amounts of these pH regulators are appropriately adjusted depending on the regulator used.
  • the pH It is preferable to add 0.1 to 5.0% by mass, preferably 0.2 to 3.0% by mass of sodium chloride to the prepared hot water or solution.
  • salt By adding salt in this way, it is possible to obtain a sweet and foliage-processed sweet potato with a brighter green color and a better flavor.
  • the heating temperature is higher than 80 ° C, preferably 90 ° C or higher.
  • the heating time is less than 5 minutes, preferably 3 minutes or less, and most preferably 10 seconds to 3 minutes.
  • the sweet potato stems and leaves After the heat treatment, it is preferable to immediately cool the sweet potato stems and leaves in order to maintain the green color and flavor. Cooling is performed by means commonly used by those skilled in the art, such as immersion in cold water or rapid cooling with cold air. The lower the cooling temperature, the greener the sweet potato stems and leaves, and the more beautiful it looks.
  • the immersion treatment in cold water is performed using water at 30 ° C or less, preferably water at 20 ° C or less.
  • the cooling time is an arbitrary time according to the processing amount of the sweet potato foliage, but it is preferably performed until the sweet potato foliage itself becomes equal to the cooling temperature.
  • the solid-liquid separation treatment is performed by, for example, squeezing sweet potato stems and leaves using a pressing machine or filtering crushed sweet potato stems and leaves. From the viewpoint of preservation, the sweet potato squeezed leaves thus obtained are preferably sterilized by heating at 80 ° C to 130 ° C. Alternatively, when it is prepared into an extract powder or the like, it is preferable to concentrate using a vacuum concentrator.
  • the extraction is carried out by adding a solvent to the cut fresh leaves of sweet potato stems or leaves (powder) or the like and keeping the mixture at a predetermined temperature.
  • the extraction solvent include water, an organic solvent, a water-containing organic solvent, and the like.
  • Examples of the water include spring water, well water, distilled water, tap water, pure water, etc. You can. From the viewpoint of extraction efficiency, it is preferable to use hot water.
  • organic solvent examples include methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, acetone, hexane, cyclohexane, propylene glycolone, ethyl methyl ketone, Lyserin, methyl acetate, ethyl acetate, getyl ether, dichloromethane, edible fats, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethane.
  • polar organic solvents such as methanol and ethanol.
  • the water-containing organic solvent is preferably used from the viewpoint of efficiently extracting the active ingredient.
  • it is a water-containing polar organic solvent, more preferably a water-containing alcohol, and further preferably a water-containing ethanol.
  • the content of water in the water-containing organic solvent is, for example, 10% by volume or more, preferably 20% by volume or more, more preferably 50% by volume or more, and further preferably 80% by volume or more.
  • hydrous ethanol containing 20% to 90% by volume of ethanol is used.
  • the amount of the extraction solvent is not particularly limited. Usually, 0.5 to 50 parts by mass of the extraction solvent is added to 1 part by mass of sweet potato leaves.
  • the extraction temperature may be appropriately set according to the type of the extraction solvent, and may be, for example, 4 ° C or more, 10 ° C or more, 25 ° C or more, 50 ° C or more, 70 ° C or more, 9 ° C or more. It can be set to 0 ° C or higher, 95 ° C or higher, 100 ° C or higher, 121 ° C or higher, and 130 ° C or higher. From the viewpoint of preventing the decomposition of the active ingredient in the sweet potato leaves, it is preferable to carry out the reaction at a low temperature.
  • the extraction may be performed under pressure depending on the extraction solvent.
  • the extraction time is appropriately set according to the extraction temperature and the like. For example, it can be appropriately set within a range of 5 minutes or more, preferably 10 minutes or more, more preferably 30 minutes or more, or 48 hours or less, preferably 24 hours or less.
  • sweet potato stems and leaves are used as they are, polyphenol in the sweet potato stems and leaves may not be sufficiently extracted.
  • 0.5 parts by mass to 50 parts by mass of water-containing ethanol having a water or ethanol content of 1 ° to 90% by volume is added to 1 part by mass of sweet potato stems and leaves. Extraction of sweet potato stems and leaves can be obtained by extracting at 00 ° C for 10 minutes to 48 hours.
  • synthetic adsorbents eg, Diaion HP20, Sephavis SP825, Amberlite XAD4, MCImage 1 CHP2OP, Chromatorex ODS
  • Dextran resin eg, Sephadex
  • a natural polyphenol separation method commonly used by those skilled in the art using LH-20 may be employed.
  • water or a water-containing polar solvent a water-containing alcohol, particularly water-containing ethanol, etc.
  • This separation method can be adopted as needed for the purpose of further purifying the sweet potato stem and leaf extract obtained above.
  • the drying and the powder frame treatment are performed for the purpose of obtaining dried sweet potato stems and leaves, dried powder, and dried extract powder.
  • Drying is performed for the purpose of long-term storage, prevention of deterioration of quality and enhancement of commercial value.
  • the drying method is not particularly limited, and examples thereof include a method using a hot air dryer, a high-pressure steam dryer, an electromagnetic wave dryer, a freeze dryer, a direct fire heater, a rotary dryer and the like. In terms of manufacturing costs and drying efficiency, hot air drying
  • drying liquids such as sweet potato squeezed leaves
  • concentration under reduced pressure and spray drying can be performed.
  • Vacuum concentration and spraying in terms of manufacturing cost and drying efficiency Drying is preferred.
  • the drying temperature is preferably about 60 ° C. to 150 ° C. under normal pressure, because a flavorful and colorful dried powder of sweet potato stems and leaves can be obtained. If the drying is performed under reduced pressure, preferably at a temperature of 60 ° C. or lower, and at a pressure at which the sweet potato stems and leaves, paste, or squeezed juice does not freeze, drying can be performed while reducing loss of nutrient components. Drying is usually performed on the basis that the moisture content in the dried product or extract powder is 5% by mass or less.
  • the sweet potato foliage when drying using a hot air dryer or the like, the sweet potato foliage may be dried as it is, but it is preferable to perform two-stage drying.
  • the two-stage drying is performed, for example, by first drying sweet potato stems or leaves at a temperature of 60 to 80 ° C. until the water content becomes 25% by mass or less, followed by primary drying.
  • the moisture content of the dried sweet potato foliage is 5% by mass or less, at a temperature higher than the primary drying, and 100 ° C or less, preferably 90 ° C or less, more preferably 85 ° C or less, More preferably, secondary drying is performed at a temperature of about 80 ° C.
  • the secondary drying temperature is 100 ° C. or less, a colorful sweet potato stem and leaf powder having a large polyphenol content can be obtained. If the primary drying temperature is lower than 60 ° C, the drying speed may be slow. If the secondary drying temperature is higher than 100 ° C, scorching may occur.
  • the temperature difference between the primary drying and the secondary drying is preferably about 5 to 15 ° C, more preferably about 10 ° C. .
  • the above spray drying is performed using a spray dryer such as a spray dryer when extracting the juice of sweet potato stems and leaves into an extract powder.
  • dextrin When using simulant drying, dextrin, cyclodextrin, This is done by adding excipients such as starch and maltose.
  • Dextrin is preferably used, and the ratio of squeezed juice to dextrin is preferably 1:10 to 5: 1 by mass in order to facilitate pulverization with dextrin-added mash.
  • a dried product (dry powder) of sweet potato stem and leaves is obtained from sweet potato stems and leaves and a heat-treated product of sweet potato stems and leaves, and a dried extract powder of sweet potato stem and leaves is obtained from sweet potato juice.
  • dried sweet potato stems and leaves obtained by drying the heat-treated sweet potato stems and leaves are suitably used as processed sweet potatoes. These dried products (especially extract powder) are used after appropriately sterilizing according to the purpose.
  • the crushing treatment is performed for the purpose of pulverizing the dried product of the sweet potato stem or leaves, or for further pulverizing the dried powder.
  • pulverization By pulverization, a uniform fine powder having a smaller particle size can be obtained. Since sweet potato stems include stems, leaves, and petiole parts having different hardnesses, it is preferable to go through a coarse pulverizing step and a fine pulverizing step from the viewpoint of increasing the efficiency of pulverization.
  • the crushed stones are cut, for example, from dried sweet potato foliage using any machine or tool known to those skilled in the art, such as a cutter, slicer, dicer and the like.
  • the size of the dried dried sweet potato stem and leaf has a major axis of 20 mm or less, preferably 0.1 to 10 mm.
  • the coarsely ground dried sweet potato stems and leaves are finely ground, but may be sterilized before the fine grinding step.
  • coarsely ground sweet potato stems and leaves can be uniformly heated, and efficient sterilization can be performed while improving the flavor of green leaves.
  • Sterilization is performed at 11 o ° C or higher using a high-pressure sterilizer, a heat sterilizer, a pressurized steam sterilizer, or the like.
  • coarsely pulverized sweet potato stem and leaf products are, for example, 0.5 to: L 0 kg / cm 2 under a pressure of 110 to 200 ° C saturated steam. For 2 to 10 seconds. If necessary, further dry the water contained during heating with saturated steam.
  • Pulverization is preferably performed so that 90% by mass passes through the 200 mesh section. Crushed.
  • the pulverization may be performed using any machine or tool usually used by those skilled in the art, such as a crusher, a mill, a blender, and a stone mill.
  • the pulverization improves the palatability, but preferably the pulverization, heating, and pulverization steps in this order further enhance the palatability.
  • the first composition of the present invention contains the processed product of the above-mentioned sweet potato stem and leaves, and the young shoot and leaf are contained in the sweet potato stem and leaves.
  • the content of the processed product of the sweet potato foliage is not particularly limited, but is preferably 0.0001% by mass or more.
  • the first composition of the present invention can be used as foods, pharmaceuticals, quasi-drugs, cosmetics, skin external preparations, and the like.
  • the processed product of the sweet potato stems and leaves can be used as it is as the first composition.For example, an extract obtained by hot-water extraction of a dry powder of sweet potato stems and leaves / sweet potato stems and leaves is directly used as tea. can do.
  • the first composition of the present invention preferably contains at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more, in addition to processed sweet potato stems and leaves.
  • the details of the combination of the processed product of the sweet potato stem and leaves and the above-mentioned saccharide will be specifically described later as the second composition.
  • the first composition of the present invention may further contain, if necessary, excipients, extenders, binders, thickeners, emulsifiers, coloring agents, flavors, food additives, seasonings, raw materials for pharmaceuticals, and the like. Other components may be included as appropriate.
  • Examples of the above food additives include royal jelly, propolis, lactic acid and derivatives thereof, vitamins (A, B, C, D, E, K, folic acid, nontothenic acid, biotin, and derivatives thereof), Minerals (iron, magnesium, calcium, zinc, etc.), selenium, chitin 'chitosan, lecithin, polyphenols (condensed tannins such as catechins, anthocyanins, proanthocyanidins, hydrolyzed tannins such as gallotannins, flavonoids) , Their derivatives, etc.), carotenoids (lycopene, astaxanthin, zeaxanthi) , Saponins (isoflavones, ginsesanoides, glycyrrhizic acid, etc.), xanthine derivatives (caffeine, etc.), fatty acids, amino acids, proteins (collagen, elastin, etc.), mucopolysaccharides (hyalur
  • vitamin B group a component for promoting metabolism of sugar such as ⁇ -lipoic acid, a component for suppressing digestion and absorption of sugar such as guava leaf, and these components
  • Food ingredients containing these; lipid absorption suppressing ingredients such as chitosan and thyrimu; lipid metabolism promoting ingredients such as L-carnitine and CoQ10; and food ingredients containing these ingredients are preferably used.
  • the excellent anti-diabetic effects obtained by containing these components are (1) the action of enhancing the secretion of insulin and the sensitivity of insulin by the stems and leaves of sweet potatoes, and (2) the component of promoting sugar metabolism or digestion of sugar.
  • the synergistic effect of the raw material having the absorption-inhibiting component or the synergistic effect of (1) and (2) and the raw material having the lipid absorption-inhibiting component or the lipid metabolism-promoting component is considered.
  • the first composition of the present invention exhibits an excellent antidiabetic effect as compared with a case where a sugar and lipid absorption-inhibiting component or a metabolic promoting component thereof is simply combined.
  • the content of the sugar metabolism promoting component, the sugar digestion and absorption suppressing component, the lipid absorption suppressing component, or the lipid metabolism promoting component in the first composition of the present invention is not particularly limited.
  • the total amount of the sugar digestion and absorption inhibitory component, the lipid absorption inhibitory component, and the lipid metabolism promoting component is 1 part by mass to 500 parts by mass, preferably 100 parts by mass of the processed product of the sweet potato stem and leaves. It is contained so as to be 5 to 250 parts by mass.
  • beverages containing the above food additives for example, fermented vegetable juice, vegetable juice (eg, ginseng juice), plant extract, fruit juice, etc., can be used.
  • a nutritious beverage can be obtained.
  • These can be seasoned by adding seasonings and the like.
  • the form of the first composition of the present invention is not particularly limited. In the case of a liquid, it can be used as a beverage as it is.
  • capsules such as hard capsules and soft capsules, tablets, or pills, or powders, granules, tea, tea bags, or candy can be formed. it can.
  • the first composition of the present invention may be eaten as it is, or may be dissolved in water, hot water, milk, or the like, depending on its shape or preference. In the case of a powdered tea bag or the like, the ingredients may be leached before drinking.
  • the first composition of the present invention is used as an external preparation for the skin (cosmetics, toiletries, etc.)
  • various base materials are added to the composition, for example, a lotion, a cosmetic toilet, a milky lotion, and a pack.
  • a second composition of the present invention comprises a processed product of sweet potato stem and leaves, a sugar alcohol and a disaccharide. It is characterized in that it contains at least one saccharide selected from the group consisting of the above saccharides.
  • sweet potato stem and leaves used in the second composition of the present invention a sweet potato stem and leaf that does not include a young stem leaf can be used, but a sweet potato stem and leaf that includes a young stem leaf may be used. From the viewpoint of obtaining a more functional composition, it is preferable to use sweet potato stems and leaves including young stems and leaves.
  • the method for preparing a processed product of sweet potato stems and leaves that does not contain young shoots is the same as described above.
  • the second composition of the present invention contains a sugar alcohol or a disaccharide or higher sugar.
  • Sugar alcohols and disaccharide or higher sugars are used alone or in combination of two or more.
  • sugar alcohol refers to a polyhydric alcohol obtained by reducing a carboxy group of a sugar molecule.
  • sugar alcohols include erythritol, pentitol, hexitol, xylitol, sorbitol, reduced palatinose, maltitol (reduced maltose), ratatitol, mannitol and the like.
  • Water-soluble sugar alcohols such as maltitol (reduced maltose) are preferred.
  • Sugar alcohol is used alone or in combination of two or more.
  • sugar or more sugars refers to those in which two or more monosaccharides are linked, and examples thereof include disaccharides, oligosaccharides, and polysaccharides (for example, starch decomposed products). No. Disaccharides or more are used alone or in combination of two or more.
  • disaccharide examples include trehalose-type disaccharides such as trehalose and sucrose (a disaccharide in which a carbon atom having a reducing group of both constituent monosaccharides is an ether bond), and manoletoses such as manoletose, cellobiose, gentiobiose, ratatose, and the like.
  • trehalose-type disaccharides such as trehalose and sucrose
  • manoletoses such as manoletose, cellobiose, gentiobiose, ratatose, and the like.
  • Disaccharide a disaccharide in which an ether bond is formed between a carbon atom having a reducing group of the first constituent monosaccharide and a carbon atom having no reducing group of the second monosaccharide
  • Oligosaccharides include isomaltooligosaccharides, bratatooligosaccharides, galacto-oligosaccharides, palatinose, maltooligosaccharides, nectar oligosaccharides, xylo-oligosaccharides, and the like.
  • Polysaccharides include homopolysaccharides composed of one kind of constituent sugars, heteropolysaccharides composed of two or more kinds of constituent sugars, neutral polysaccharides, acidic polysaccharides, glycosaminodalican, dextrin, starch, amylose, amylopectin, and cellulose.
  • indigestible dextrin Preferred are indigestible dextrins.
  • the indigestible dextrin is an indigestible polysaccharide obtained from starch, and is obtained, for example, by hydrolyzing starch by heating and then hydrolyzing it with amylase. Mainly has four bonds, which starch originally has.
  • Indigestible dextrin is said to have the effect of suppressing postprandial blood glucose elevation, the effect of improving blood lipids, and the effect of intestinal regulation.
  • Indigestible dextrin is commercially available in the form of powder, fine granules, granules, and the like, and any form can be used. Indigestible dextrin is also used in the form of an aqueous solution because it is water-soluble.
  • water-soluble sugars such as oligosaccharides, dextrins and indigestible dextrins are preferred, and more preferably indigestible dextrins.
  • the average molecular weight is 9,000 or less (average molecular weight when converted to Darcos). It is preferred that the number of monosaccharides linked is 50 or less).
  • the structure of the disaccharide or more saccharide used in the second composition of the present invention is not particularly limited, and a saccharide to which a monosaccharide is bonded to form a cyclic structure (for example, cyclodextrin or the like) Etc.), sugars that form a chain structure by combining monosaccharides (maltose, amylose, cellulose, etc.), or sugars that form a branched structure by combining monosaccharides (amylopectin, polysaccharide) Dextrose).
  • a saccharide to which a monosaccharide is bonded to form a cyclic structure for example, cyclodextrin or the like
  • sugars that form a chain structure by combining monosaccharides maltose, amylose, cellulose, etc.
  • sugars that form a branched structure by combining monosaccharides asmylopectin, polysaccharide
  • the second composition of the present invention contains a processed product of a sweet potato foliage and at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more.
  • the second composition of the present invention can be used as foods, pharmaceuticals, quasi-drugs, cosmetics, external preparations for skin, etc., particularly as foods or pharmaceuticals.
  • the content of the processed product of the foliage of sweet potato in the second composition of the present invention is not particularly limited.
  • it is preferably 0.0001% by mass or more.
  • the content is preferably 0.0001% by mass to 50% by mass / 0 .
  • the sweet potato foliage is used as it is as a dry powder, it is used in an amount of 0.01 to 80% by mass, preferably 0.01 to 50% by mass. / 0 .
  • the content of sugar alcohol and sugars higher than disaccharide in the second composition of the present invention is not particularly limited.
  • the amount is 10 to 2000 parts by mass, more preferably 10 to 1000 parts by mass, based on 100 parts by mass of the processed product of sweet potato foliage.
  • sugars such as sugar alcohols and disaccharides are usually contained in the composition preferably in an amount of 3% by mass or more, more preferably 5% by mass or more.
  • the second composition of the present invention may contain, as necessary, other components used in the first composition, in addition to the processed product of the sweet potato stem and leaves, sugar alcohol, and disaccharide or more saccharides.
  • the dosage form of the second composition of the present invention is not particularly limited, and can be formed in the same form as the first composition.
  • Preferred are pastes, gels, and solid granules (granules, fine granules, tablets, kneaded materials, baked goods, etc.), and more preferably granules. Since the second composition of the present invention has excellent granulation properties, as described above, Particularly, it is suitably used as a granulated product.
  • Granulation methods include commonly used granulation methods, for example, tumbling granulation method, mixing stirring granulation method, extrusion granulation method, fluidized bed granulation method, emulsion granulation method, fluidized granulation, spray dryer It can be formed using a granulation method using a coating, a coating granulation method, or the like.
  • the intake of the second composition of the present invention is not particularly limited.
  • the daily intake of an adult is 10 mg to 300 mg, preferably 10 mg to: L as the total amount of dicaffeoylquinic acid and the like contained in the processed product of sweet potato foliage. It is taken so as to be 0.000 mg.
  • a dry powder of sweet potato stems and leaves it may be blended in an amount of 0.1 g to 30 g, preferably 0.1 to 10 g.
  • the second composition of the present invention exhibits excellent granulation properties and stability, and has a palatability as compared with the case where a saccharide other than saccharide alcohol or disaccharide is contained.
  • the effect of suppressing an increase in blood glucose level is also superior to the case of containing a sugar alcohol and a sugar other than disaccharide or more.
  • the composition of the present invention contains a processed product of sweet potato stems and leaves, it has a high polyphenol content and is excellent in nutritional value.
  • the first composition contains the sweet potato stem and leaves in the sweet potato stem and leaf, the polyphenol content is higher and the processing is easy.
  • the second composition further contains at least one selected from the group consisting of sugar alcohols and disaccharides and higher sugars, various formulations containing a processed sweet potato product are only possible. In addition, palatability and stability are improved.
  • a sweet potato-containing solid eg, a granulated substance such as a powder, a key material, or a granule
  • the second composition of the present invention has a particularly excellent antidiabetic effect.
  • Example 1 Production of a composition containing a processed product of young shoots of sweet potato
  • Suio seeds were planted and cultivated until the length of the aerial part was about 150 cm. A portion of 60 cm from the tip of the sweet potato leaf was cut off and washed twice with water to obtain a 1 kg young shoot of sweet potato (referred to as sweet potato stem 1).
  • the obtained young shoots of sweet potatoes were cut into about 5 mm and immersed in 2 L of hot water (90 ° C.) adjusted to pH 8.0 for 1 minute. Then, after cooling with water at 25 ° C, the mixture was centrifuged for 30 seconds and dehydrated to some extent. Then, dry in a dryer at 70 ° C for 2 hours with hot air (primary drying) until the water content becomes approximately 20% by mass. Drying with warm air at ° C for 4 hours
  • Example 2 The sweet potato stems and leaves that were cut off in Example 1 were further cultivated until the length of the above-ground portion became about 150 cm. Then, a portion 60 cm from the tip of the leaf of the sweet potato was cut off again to obtain a young shoot of the sweet potato (referred to as a sweet potato stem 2). Using the young shoots of this sweet potato, the same procedure as in Example 1 was carried out to obtain 80 g of sweet potato shoot powder. The above operation was repeated again to obtain a total of 160 g of sweet potato stem and leaf dry powder. The total amount of polyphenol was measured as described above. Table 1 shows the results. (Example 3)
  • Example 1 Instead of the young shoots and leaves of the sweet potato of Example 1, all the above-ground portions of the sweet potatoes and leaves cultivated in the same manner as in Example 1 were cut off (that is, the sweet potatoes and leaves including the young shoots were cut off), and the sweet potatoes and leaves (full length) were obtained. (Sweet potatoes and leaves 3) Except that this was used as sweet potato stems and leaves, the same procedure as in Example 1 was carried out to obtain 100 g of dry powder of sweet potato stems and leaves from 1 kg of sweet potato stems and leaves. The total amount of polyphenol contained in the obtained dry powder was measured. The results are shown in Table 1.
  • Example 1 Instead of the young shoots of the sweet potatoes of Example 1, all the above-ground parts of the sweet potatoes and leaves cultivated in the same manner as in Example 1 were cut off, and a portion of 60 cm from the tip of the sweet potatoes and leaves was removed to obtain sweet potato shoots. (Sweet potatoes and leaves 4). Except for using the sweet potato stems and leaves, the same procedure as in Example 1 was carried out to obtain 110 g of a dry powder of sweet potato stems and leaves from 1 kg of sweet potato stems and leaves, and the total polyphenol content was measured. . Table 1 shows the results.
  • the young stem leaves of sweet potatoes (the part 60 cm from the tip of the sweet potato leaves) It can be seen that the dry powders containing Examples (Examples 1 to 3) have a higher polyphenol content than the dry powders containing no young shoot leaves of the sweet potato of Preparation Example 1. In particular, the young stem and leaf portions generated from the once cut portion of Example 2 also had a high polyphenol content. This indicates that the young stems and leaves of the sweet potatoes (especially the portion 60 cm from the tip of the sweet potato leaves) contain a large amount of the active ingredient, especially polyphenol.
  • the dry powders of Examples 1 to 3 had a better palatability than the dry powders of the Preparation Examples, and particularly the dry powders of Examples 1 and 2 had a better palatability. This is considered to be because the stem portions of the young shoots of the sweet potato used in Examples 1 to 3, particularly Examples 1 and 2 are lucid.
  • Example 2 To 3 g of the young shoots and leaves of the sweet potato obtained in Example 1 (sweet shoots and leaves 1) was added 100 mL of an aqueous solution of 80% by volume of ethanol, and the mixture was heated and refluxed at 80 ° C for 24 hours. I got it. Thereafter, filtration was performed to obtain an extract, which was further concentrated under reduced pressure at 50 ° C. to obtain an extract powder. Using 100 mg of this extracted powder, the total amount of polyphenol per 100 g of the extracted powder was measured using catechin as a standard substance in the same manner as in Example 1. The same procedure was applied to the sweet potato stems and leaves 2 to 4 of Example 2 and Preparation Example 1 to measure the mass of the extracted powder and the total amount of polyphenol per 100 g of the extracted powder. Table 2 shows the results.
  • a drink course was prepared according to the following procedure. First, 100 g of the extracted powder of the sweet potato stem and leaf 1 obtained in Example 6 was extracted with 1 L of hot water for 30 minutes, and then filtered to obtain a hot water extract of the sweet potato stem and leaf 1. As above, extract 10 g of green tea leaves and 10 g of black tea leaves with 1 L of hot water •-15 each, filter and extract hot water extracts of green tea leaves and black tea leaves. Obtained. Using these extracts and ascorbic acid, a tea drinking department was prepared with the composition shown in Table 5. Table 5
  • a sweet potato stem fine powder was prepared as follows. First, the surface power including the young shoots: 4 kg of sweet potato stems and leaves grown to a height of 200 cm were harvested (referred to as sweet potato stems and leaves 5), immersed in hot water (97 ° C) adjusted to pH 8.0 for 1 minute, and subjected to blanching treatment. Immediately after the blanching treatment, it was immersed in water at 20 ° C to cool, and then dried with hot air. Next, the powder is ground with a dicer so that the major axis is about lmm, sterilized by a pressurized steam sterilizer, and 90 mass% or more of the particle diameter of the powder is 600 mesh or less (20 ⁇ m or less) using a hammer mill. And pulverized so that The resulting fine powder of the sweet potato stems and leaves 5 was 3.2 kg.
  • a mixed powder having the composition shown in Table 8 was prepared using the fine powder of sweet potato stem and leaf 5 obtained in Example 12, starch, reduced maltose, trehalose, indigestible dextrin, and fructose. Fluid granulation was performed using 700 g of the mixed powder. In the fluidized granulation, 10 mL of water per minute was sprayed for 15 minutes at an air supply temperature of 95 ° (:, exhaust temperature of 30 ° C.), followed by drying for 20 minutes to obtain powders 1 to 4. Each of the obtained powders (powder 1 to 4) was evaluated for granulation properties, dispersibility in water, and palatability as follows.
  • the powder after the fluid granulation was sieved using a sieving machine equipped with a 140-mesh (106 ⁇ m) sieve, and whether or not granules were formed was determined according to the following criteria.
  • the passing amount of the powder is less than 30% by mass (very good granulation): ⁇ The passing amount of the powder is 30% to 50% by mass (good granulation): ⁇ Passing amount of powder exceeds 50% by mass (poor granulation): X
  • the powders 1 to 3 using the fine powder of sweet potato stems and leaves and sugar alcohol or sugars other than disaccharide of the present invention have particularly excellent granulation properties and excellent dispersibility in water.
  • Example 15 Production of tablets
  • Extract powder of sweet potato stem and leaf 5 obtained in Example 14 obtained in Example 14, crystalline cellulose, sucrose Ester, silicon dioxide, maltose, erythritol, palatinose, 'fructooligosaccharide, and fructose were prepared into tablets (200 mg per tablet) according to the composition shown in Table 9.
  • Example 16 Preventive and therapeutic effects on type I diabetes (suppressing or lowering blood sugar levels)
  • Example 1 with standard mouse feed (MF, Oriental Yeast Co., Ltd.)
  • test powders 1-3 were prepared by mixing the fine powder of the sweet potato stems and leaves 5 obtained in Step 2, dextrin (manufactured by Matsutani Chemical Industry Co., Ltd.), reduced maltose, and fructose so as to have the contents shown in Table 10. did.
  • dextrin manufactured by Matsutani Chemical Industry Co., Ltd.
  • reduced maltose reduced maltose
  • fructose so as to have the contents shown in Table 10. did.
  • the effect on type I diabetes was examined as follows.
  • mice 28-week-old male type 2 diabetes model mice (KK-Ay mice) (Charles River Japan, Inc.) were acclimated to a standard diet for 28 days, blood was collected from the fundus, and blood glucose was measured. Was measured using a dull test sensor (manufactured by Sanwa Kagaku).
  • the mice were divided into four groups, seven per group, so that the average blood glucose level was uniform (255 to 257 mg / dL). Three groups of rats were allowed to freely take test feeds 1-3. The remaining one group of rats had free access to a control diet in which fructose was mixed with the standard diet. Water during the test was free drinking. Blood was collected from the orbital vein on the 28th day from the start of ingestion, and the blood glucose level in the blood was measured. The results are shown in Table 10.
  • the blood glucose values in Table 10 represent the mean soil standard deviation (mg_dL).
  • the processed sweet potato leaves containing the young shoots and leaves were found to have the effect of suppressing the increase in blood sugar level due to fructose. Understand.
  • the test feeds 1 and 2 containing the processed product (fine powder) of sweet potato stems and leaves and a sugar alcohol or a disaccharide or more sugars have an even more excellent blood sugar level suppressing effect. That is, it is found that the composition containing the processed product of the sweet potato stem and leaves of the present invention and a sugar alcohol or a disaccharide or higher saccharide has a particularly excellent antidiabetic effect (the effect of preventing or treating diabetes).
  • Fine powder of sweet potato stems and leaves obtained in Example 17 (raw material having dicaffeoylquinic acid, etc.), guava leaf extract (trade name: guaba phenone, Bizen Kasei Neyring), indigestible dextrin (trade name) : Pine fiber, manufactured by Matsutani Chemical Industry Co., Ltd.), vitamin B1, and reduced maltose were used to prepare foods (powder) with compositions shown in Table 1.1 (foods 1 to 4, respectively).
  • Guava extract, indigestible dextrin, and vitamin B are raw materials having a sugar metabolism promoting component or a sugar digestion / absorption inhibiting component.
  • foods 1 to 3 were used to evaluate the antidiabetic effect as follows.
  • test group Two of the groups were given oral gavage of foods 1 and 2 once a day for 7 days so that each of them weighed 100 OmgZkg body weight (test group). In each oral administration, 0.5% Tween physiological saline was used as a solvent. In another group, food 3 was administered in the same manner as described above (comparative test group). The other group received only vehicle (control group). During the test, the standard feed was freely available and water was also freely available. Seven days after ingestion, blood was again collected at the time of feeding, and blood glucose (mg / dL) in the blood was measured. Table 12 shows the results (mean and standard deviation). Table 12
  • Zika Foods containing a raw material containing hueoylquinic acid and indigestible dextrin contain indigestible dextrin having an effect of improving bowel movement, so that improvement in bowel movement can also be expected.
  • Example 19 Analysis of sweet potato stem dry powder
  • the total polyphenol amount in terms of caffeic acid in the dried powder of the sweet potato stem and leaf 1 obtained in Example 1 was measured as follows. First, lOOOmL of water was added to 100mg of the dry powder, and ultrasonic extraction was performed for 20 minutes. The resulting extract was measured for absorbance at 70 nm by the Folin-Dennis method. In addition, a commercially available cuffic acid (Sigma-Aldrich Japan KK) was used as a standard substance. A calibration curve was prepared by measuring the absorbance at 730 nm in the same manner as described above, and the total amount of polyphenol was calculated using this curve in terms of caffeic acid.
  • the processed product of sweet potato stems and leaves contained in the composition of the present invention contains the active ingredient contained in the sweet potato stems and leaves, particularly polyphenol, by containing young leaves of sweet potato stems and leaves. It can be used as a raw material for cosmetics.
  • a composition containing such a processed product of sweet potato stems and leaves contains nutrients of sweet potato stems and leaves and is not only excellent in palatability, but also contains many polyphenol components, and is expected to have various physiological effects. Yes, highly useful.
  • by combining the processed product of sweet potato foliage with at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more granulation, stability, palatability, and anti-diabetic effects can be improved. An excellent composition can be obtained.

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Abstract

A composition containing processed sweet potato foliage including young sweet potato foliage. Because of containing active components (in particular, polyphenols) in a large amount, the processed sweet potato foliage contained in the composition is usable as the starting material for foods, drugs, quasi drugs, cosmetics and so on. The composition containing the processed sweet potato foliage contains the nutritional components of the sweet potato foliage and has a favorable taste. Moreover, it contains many polyphenol components and, therefore, is expected as exerting various physiological effects and high usefulness.

Description

甘藷茎葉加工物含有組成物  Composition containing processed sweet potato foliage
技術分野 Technical field
本発明は、 甘藷茎葉の加工物を含有する組成物に関し、 より詳細には、 甘 藷の若茎葉 (特に、 甘藷の葉の先端部から 6 0 c m以下の部位) を含有する 明  The present invention relates to a composition containing a processed product of a sweet potato stem and leaves, and more particularly, a composition containing a young shoot of a sweet potato (particularly, a site 60 cm or less from the tip of the sweet potato leaf).
組成物に関する。 書 Composition. book
背景技術 Background art
ヒルガオ科に属する甘藷 (さつまいも) の茎葉は、 食料の豊富な現代にお いては、 ほとんど食されておらず、 その有効活用が求められている。  Sweet potatoes belonging to the convolvulaceae family are hardly eaten in modern food-rich days, and their effective use is required.
近年、 この甘藷の茎葉は、 ビタミン、 ミネラルなどの健康に有効な成分を 含有することが明らかにされ、 生活習慣病予防を目的とした食品原料として 利用され始めている。 例えば、 特開平 7— 1 1 1 8 7 8号公報には、 甘藷の 茎葉を水に浸漬、 煮沸し、 溶出液を濃縮し、 得られたエキスを海苔に塗布し た力リゥム含有食品が記載されている。 特開平 6 - 6 2 7 8 3号公報には、 甘藷茎葉の処理方法として、 甘藷茎葉を洗浄する際に、 含有されるビタミン、 ミネラルなどの有効成分を流出させないように、 噴出水で洗浄し、 乾燥、 粉 末ィヒすることが記載されている。  In recent years, the foliage of this sweet potato has been shown to contain vitamins, minerals and other health-effective ingredients, and has begun to be used as a food ingredient for the prevention of lifestyle-related diseases. For example, Japanese Unexamined Patent Publication (Kokai) No. 7-111878 describes a foodstuff containing sweet potato obtained by immersing the stems and leaves of a sweet potato in water, boiling it, concentrating the eluate, and applying the obtained extract to nori. Have been. Japanese Patent Application Laid-Open No. 6-627883 discloses a method for treating sweet potato stems and leaves by washing with spouted water so as to prevent effective ingredients such as vitamins and minerals contained therein from flowing out when washing the sweet potato stems and leaves. It is described that drying, drying and powdering are performed.
しかし、 甘藷茎葉には、 上記文献で着目される成分以外にも、 種々の優れ た成分、 例えば、 ポリフエノールなどが含有されている。 そのため、 甘藷茎 葉の有効利用という点では未だ不十分である。 さらに、 甘藷茎葉の茎部は硬 い繊維質であるため、 粉末化などの製剤化が困難であること、 その結果、 適 用範囲が食品または医薬品の一部に限られるなどの問題点、 あるいは甘藷茎 葉に含まれる成分が不安定であることなどの問題点もある。 発明の開示 However, the sweet potato stems and leaves contain various excellent components, such as polyphenol, in addition to the components noted in the above literature. Therefore, it is still insufficient in terms of effective use of sweet potato stems and leaves. In addition, since the stem of sweet potato stems and leaves are hard fibrous materials, it is difficult to formulate such as powder, and as a result, the applicable range is limited to a part of foods or pharmaceuticals. There are also problems such as the instability of the components contained in the sweet potato stems and leaves. Disclosure of the invention
本発明の目的は、 加工しやすく、 有効成分量、 特にポリフエノール含有量 が高い甘藷 葉の加工物を提供することにある。  An object of the present invention is to provide a processed product of sweet potato leaves which is easy to process and has a high active ingredient content, particularly a high polyphenol content.
本願発明者らは、 有効成分量、 特にポリフエノール含有量が高い甘藷茎葉 の加工物について、 鋭意検討を行った。 その結果、 甘藷茎葉の若茎葉を用い ることによって、 加工が容易であり、 有効成分が豊富な甘藷茎葉の加工物が 得られることを見出した。 さらに、 甘藷茎葉の加工物と、 糖アルコールまた は二糖以上の糖とを組み合わせることにより、 優れた造粒性、 保存安定性、 嗜好性、 および糖尿病予防効果が得られることを見出して、 本発明を完成す るに至った。  The inventors of the present application have conducted intensive studies on a processed product of sweet potato stems and leaves having a high active ingredient content, particularly a high polyphenol content. As a result, it was found that by using the young shoots of sweet potato stems and leaves, processing was easy and a processed product of sweet potato stems and leaves rich in active ingredients was obtained. Furthermore, it was found that by combining a processed product of sweet potato foliage with a sugar alcohol or a disaccharide or higher sugar, excellent granulation properties, storage stability, palatability, and diabetes prevention effects were obtained. The invention has been completed.
すなわち、 本発明の組成物は、 甘藷茎葉の加工物を含有し、 該甘藷茎葉中 に若茎葉が含まれる。  That is, the composition of the present invention contains a processed product of sweet potato stems and leaves, and the young shoots are contained in the sweet potato stems and leaves.
好ましい実施態様においては、 上記甘藷茎葉は、 地上部の長さが 3 0 0 c m以下の甘藷茎葉の地上部の少なくとも一部である。  In a preferred embodiment, the sweet potato leaves are at least a part of the above-ground portions of sweet potato leaves having a length of 300 cm or less.
好ましい実施態様においては、 上記甘藷の若茎葉は、 甘藷の葉の先端から 6 0 c m以下の部位である。  In a preferred embodiment, the young stem leaf of the sweet potato is a site of 60 cm or less from the tip of the sweet potato leaf.
本発明の組成物はまた、 甘藷茎葉の加工物と、 糖アルコールおよびニ糖以 上の糖からなる群より選択される少なくとも 1種の糖類とを含有する。  The composition of the present invention also contains a processed product of sweet potato foliage and at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or higher.
好ましい実施態様においては、 上記甘藷茎葉中に若茎葉が含まれる。  In a preferred embodiment, young shoots are contained in the sweet potato shoots.
好ましい実施態様においては、 上記糖類は、 難消化性デキストリンである。 発明を実施するための最良の形態  In a preferred embodiment, the saccharide is an indigestible dextrin. BEST MODE FOR CARRYING OUT THE INVENTION
本発明の組成物は、 甘藷茎葉の加工物を含有し、 該甘藷茎葉中には若茎葉 が含まれる (以下、 第 1の組成物という) 。 あるいは甘藷茎葉の加工物と、 糖アルコールぉよび二糖以上の糖からなる群より選択される少なくとも 1種 の糖類とを含有し、 好ましくは該甘藷茎葉中に若茎葉が含まれる (以下、 第 2の糸且成物という) 。 以下、 まず、 第 1の組成物および第 2の糸且成物に用い られる甘藷茎葉およびその加工物について説明する。 ( 1 ) 甘藷茎葉 The composition of the present invention contains a processed product of sweet potato stems and leaves, and the sweet potato stems and leaves include young shoots (hereinafter, referred to as a first composition). Alternatively, at least one selected from the group consisting of a processed product of sweet potato stem and leaves and a sugar alcohol and a sugar of two or more sugars The sweet potatoes preferably contain young shoots (hereinafter, referred to as a second itotsuto product). Hereinafter, first, the sweet potato stem and leaves used in the first composition and the second thread and the processed product thereof will be described. (1) Sweet potato stems and leaves
本発明に用いられる甘藷茎葉において、 その甘藷の品種は特に限定されな レ、。 例えば、 ジョイホワイト、 コガネセンガン、 シロユタカ、 サッマスター チ、 アヤムラサキ、 すいおうなどが挙げられる。 そのうち、 本発明では、 特 定品種である 「すいおう」 の茎葉が好適に用いられる。 特に、 「すいおう」 の茎葉は、 ポリフエノール含有量が高く、 一度茎葉を収穫した後であっても、 再度茎葉が再生するという特徴を有する。 さらに、 すいおうは、 再生した茎 葉においても、 ポリフエノールを豊富に含んでいる。 これらの理由により、 本発明では、 すいおうの甘藷茎葉を用いることが特に好ましい。  In the sweet potato stems and leaves used in the present invention, the variety of the sweet potato is not particularly limited. For example, Joy White, Koganesengan, Shiroyutaka, Sama Starch, Ayamurasaki, and Suiou. Among them, in the present invention, the foliage of "Suiou" which is a special variety is preferably used. In particular, the foliage of "Suiou" has a high polyphenol content, and is characterized in that foliage is regenerated again even after the foliage is once harvested. In addition, Suio is rich in polyphenols even in regenerated foliage. For these reasons, it is particularly preferred in the present invention to use sweet potato foliage.
本明細書において、 「甘藷茎葉」 とは、 「甘藷の茎および葉の少なくとも 一部」 をいう。 ここで、 甘藷の茎は、 地上部の茎おょぴ地下部の茎 (地下 茎) のいずれも含み得るが、 地上部の茎が好ましく用いられる。 このように、 本発明においては、 甘藷の地上部の茎葉、 すなわち甘藷栽培時に出る地上部 の茎および葉が好適に用いられる。  In the present specification, the term "sweet potato stem and leaves" refers to "at least a part of the stem and leaves of sweet potato". Here, the stalk of the sweet potato may include any of the above-ground stalk and the underground stalk (underground stalk), but the above-ground stalk is preferably used. As described above, in the present invention, the shoots and leaves of the aerial part of the sweet potato, that is, the stems and leaves of the aerial part that are produced during sweet potato cultivation are preferably used.
本発明に用いられる甘藷茎葉の大きさは、 特に制限されない。 し力 し、 甘 藷茎葉は、 成長に際し、 特に茎部が大きく成長することが知られているとこ ろ、 このような成長した甘藷茎葉は、 得られる加工物の収量が多い点で好ま しいが、 その一方で、 加工しにくくなる、 あるいは得られる加工物の栄養価、 特にポリフエノール含有量が少なくなる場合がある。 したがって、 通常、 所 定の大きさの甘藷茎葉を用いることが好適である。 例えば、 地上部の長さ、 すなわち甘藷の茎が地中から外に出ている位置 (地表) ら甘藷の葉の先端 までの長さにおいて、 好ましくは 1 0 c m以上、 より好ましくは 3 O c m以 上、 さらに好ましくは 40 cm以上、 最も好ましくは 60 cm以上であり、 好ましくは 300 cm以下、 より好ましくは 200 cm以下、 さらに好まし くは 150 cm以下の甘藷茎葉が用いられる。 地上部の長さが 300 cmを 超えると、 甘藷茎葉の先端部が地面につきやすくなつてしまい、 害虫などの 害を受けやすく、 害を受けた場合に、 十分量の甘藷茎葉を収穫できなくなる おそれがある。 The size of the foliage of the sweet potato used in the present invention is not particularly limited. When it is known that the sweet potato stems and leaves grow particularly large when growing, such grown sweet potato stems and leaves are preferable in terms of a large yield of processed products obtained. On the other hand, processing may be difficult, or the resulting processed product may have a reduced nutritional value, especially the polyphenol content. Therefore, it is usually preferable to use sweet potato stems and leaves of a predetermined size. For example, the length of the above-ground portion, that is, the length from the position where the sweet potato stems are out of the ground (ground surface) to the tip of the sweet potato leaf, is preferably 10 cm or more, and more preferably 30 cm. Less than Above, more preferably 40 cm or more, most preferably 60 cm or more, preferably 300 cm or less, more preferably 200 cm or less, even more preferably 150 cm or less, is used. If the length of the aerial part exceeds 300 cm, the tip of the sweet potato stems and leaves will easily touch the ground, and it will be susceptible to pests and other insects, and if so, it may not be possible to harvest a sufficient amount of sweet potato stems and leaves There is.
上記甘藷茎葉としては、 さらに緑色を保持した甘藷茎葉を用いることが好 ましい。 このような甘藷茎葉およぴ甘藷茎葉の若葉は、 植物体自身がやわら かいため、 加工が容易であり、 そのまま乾燥粉末などに加工した場合、 舌触 りのよい乾燥粉末を得ることができる。  As the above-mentioned sweet potato stems and leaves, it is preferable to use sweet potato stems and leaves further retaining green. Such sweet potato stems and leaves are easy to process because the plant itself is soft, and when processed directly into dry powder etc., a dry powder with a pleasant texture can be obtained. .
本発明の組成物に用いられる甘藷茎葉は、 若茎葉を含んでいてもよい。 特 に、 第 1の組成物にはこの若茎葉が必須である。 この甘藷の 「若茎葉」 とは、 甘藷茎葉の先端部分にあり、 他の茎葉に比べて、 黄味がかった緑色を有する 部位をいう。 具体的には、 最先端の茎または葉から好ましくは 60 cm以下、 より好ましくは 40 cm以下、 さらに好ましくは 20 cm以下、 最も好まし くは 10 cm以下の部位である。 この甘藷の若茎葉は、 ポリフエノールを多 く含有するため、 甘藷茎葉の中でも特に好ましく用いられる。 本発明に用い られる甘藷茎葉は、 この若茎葉を好ましくは乾燥質量換算で 0. 1質量%以 上、 より好ましくは 1質量%以上含有する。  The sweet potato foliage used in the composition of the present invention may include a young foliage. In particular, the young shoots are essential for the first composition. The term "young shoots" of the sweet potato refers to a site at the tip of the sweet potato stem and leaves, which has a yellowish green color compared to other stems and leaves. Specifically, the site is preferably 60 cm or less, more preferably 40 cm or less, even more preferably 20 cm or less, and most preferably 10 cm or less from the most advanced stem or leaf. Since the young shoots of sweet potatoes contain a large amount of polyphenol, they are particularly preferably used among sweet potato shoots. The sweet potato foliage used in the present invention contains the young foliage preferably in an amount of 0.1% by mass or more, more preferably 1% by mass or more in terms of dry mass.
本発明に用いられる甘藷茎葉は、 さらにジカフェオイルキナ酸おょぴトリ カフェオイルキナ酸 (以下、 ジカフェオイルキナ酸等という) を豊富に含む ことが好ましい。 甘藷茎葉 (特にすいおうの茎葉) は、 一般に、 他の植物と 比べて、 トリカフェオイルキナ酸を多く含んでいる。 例えば、 乾燥質量 10 0 gあたり、 トリカフェオイルキナ酸を 2mg〜300mg、 好ましくは 1 0mg〜30 Omg含む甘藷茎葉が好適に用いられる。 さらにジカフェオイ ルキナ酸を 100mg〜600 Omg含有する甘藷茎葉が好適である。 この ようなジカフェオイルキナ酸等を含有する甘藷茎葉としては、 好ましくはす いおうの茎葉、 特に好ましくはすいおうの若茎葉である。 It is preferable that the stem and leaves of the sweet potato used in the present invention further contain a large amount of dicaffeoylquinic acid and tricaffeoylquinic acid (hereinafter referred to as dicaffeoylquinic acid and the like). Sweet potato stems and leaves (especially sugarcane stems and leaves) generally contain more tricaffeoylquinic acid than other plants. For example, sweet potato foliage containing 2 mg to 300 mg, preferably 10 mg to 30 Omg of tricaffeoylquinic acid per 100 g of dry mass is suitably used. Further, sweet potato stems and leaves containing 100 mg to 600 mg of dicaffeoylquinic acid are preferred. this The sweet potato foliage containing such dicaffeoylquinic acid is preferably a foliage foliage, particularly preferably a foliage young foliage.
本発明に用いられる甘藷茎葉は、 通常、 泥などを水で洗浄した後、 加工に 供される。  The stem and leaves of the sweet potato used in the present invention are usually subjected to processing after washing mud and the like with water.
( 2 ) 甘藷茎葉の加工物およびその調製 (2) Processed sweet potato leaves and their preparation
本発明に用いられる甘藷茎葉の加工物は、 上記甘藷茎葉またはその洗浄物 を処理したものであれば特に制限されない。 このような甘藷茎葉の加工物は、 甘藷茎葉を、 例えば、 破碎、 加熱処理、 固液分離、 抽出、 乾燥、 および粉末 化処理からなる群より選択される少なくとも 1種で処理することによって得 られる。 具体的には、 甘藷茎葉の破砕物、 加熱処理物、 搾汁、 抽出物、 抽出 液、 乾燥物、 乾燥粉末、 乾燥エキス末などが挙げられる。 以下、 上記の処理 方法 (破碎、 加熱処理、 固液分離処理、 抽出、 乾燥、 およぴ粉碎処理) につ いて説明する。  The processed product of the sweet potato stem and leaves used in the present invention is not particularly limited as long as the sweet potato stem and leaves or the washed product thereof is treated. Such a processed product of sweet potato stems and leaves is obtained by treating sweet potato stems and leaves with, for example, at least one selected from the group consisting of crushing, heat treatment, solid-liquid separation, extraction, drying, and powdering. . Specific examples include crushed sweet potato stems and leaves, heat-treated products, squeezed juices, extracts, extracts, dried products, dried powders, and dried extract powders. Hereinafter, the above-mentioned treatment methods (crushing, heat treatment, solid-liquid separation treatment, extraction, drying, and grinding treatment) will be described.
( 2 - 1破砕)  (2-1 crush)
破碎は、 フードプロセッサ、 マスコ口イダーなどの当業者が通常用いる破 砕手段を用いて行われる。 破薛を容易にする観点から、 フードプロセッサな どで粗く破碎した後に、 マスコ口イダーを用いてさらに細かく破砕すること が好ましい。 破碎により、 例えば、 長径が 0 . 1 mn!〜 2 0 mm程度の甘藷 茎葉の破碎物が得られる。  The crushing is performed using a crushing means commonly used by those skilled in the art, such as a food processor and a masco mouth lider. From the viewpoint of facilitating crushing, it is preferable to use a food processor or the like to coarsely crush and then further crush using a Masco mouth ider. Due to crushing, for example, the major axis is 0.1 mn! Fragments of sweet potato stems and leaves of about 20 mm can be obtained.
( 2 - 2 :加熱処理)  (2-2: Heat treatment)
加熱処理は、 甘藷茎葉中の酵素を失活させ、 品質を安定させること、 ある いは甘藷茎葉の褪色を防ぐ目的で行われる。 加熱処理としては、 例えば、 プ ランチング処理 (湯通し) 、 乾熱処理、 マイクロウエーブ処理、 赤外線や遠 赤外線処理、 水蒸気処理等が挙げられる。 好ましくはプランチング処理であ る。 このプランチング処理は、 クロロフィルの色の褪色を防止する目的で当 業者が通常行う方法、 特に湯通しが好適に採用される。 加熱処理は、 甘藷茎 葉およびその加工物 (例えば、 破碎物、 搾汁、 乾燥粉末、 乾燥エキス末な ど) のいずれに対しても適宜行うことができる。 甘藷茎葉をそのまま加熱処 理する場合は、 処理工程の便宜上、 必要に応じて長径が 1 mm〜 3 0 c m程 度に切断して用いることが好ましい。 The heat treatment is performed to deactivate enzymes in the sweet potato stems and leaves and stabilize the quality, or to prevent fading of the sweet potato stems and leaves. Examples of the heat treatment include a plating treatment (blanching), a dry heat treatment, a microwave treatment, an infrared or far-infrared treatment, and a steam treatment. Preferably, it is a planting process. This planting process is performed to prevent the color of chlorophyll from fading. A method usually performed by a trader, particularly desalination, is suitably employed. The heat treatment can be appropriately performed on sweet potato stems and leaves and processed products thereof (eg, crushed material, squeezed juice, dried powder, dried extract powder, etc.). When the sweet potato stems and leaves are heat-treated as it is, it is preferable to cut and use the major diameter of about 1 mm to 30 cm as necessary for convenience of the treatment step.
上記加熱処理は、 用いる甘藷の種類によって条件が大きく異なり、 さらに 風味や栄養素が損なわれたり、 有用成分の生理活性が失活しやすいなどの問 題が起こりやすい。 本発明では、 p H 5 . 4以上、 好ましくは p H 5 . 6以 上 8 . 4未満、 より好ましくは 5 . 6〜8 . 0、 最も好ましくは 5 . 6〜7 . 6で加熱処理を行う。 これによつて、 有効成分 (例えば、 トリカフェオイル キナ酸、 ジカフェオイルキナ酸などのポリフエノールなど) の含有量が高い 甘藷茎葉加工物を得ることができる。  The conditions of the heat treatment vary greatly depending on the type of sweet potato used, and furthermore, problems such as impairment of flavor and nutrients, and susceptibility to inactivation of useful components of physiological activity are likely to occur. In the present invention, the heat treatment is performed at pH 5.4 or more, preferably pH 5.6 or more and less than 8.4, more preferably 5.6 to 8.0, and most preferably 5.6 to 7.6. Do. Thereby, a processed sweet potato foliage having a high content of an active ingredient (for example, polyphenol such as tricaffeoylquinic acid or dicaffeoylquinic acid) can be obtained.
特にブランチング処理の場合は、 上記有効成分が高い甘藷茎葉加工物を得 る観点力 ら、 上記 p Hの熱水を用いて行うことが好ましい。 また、 乾熱処理、 マイクロウエーブ処理、 赤外線または遠赤外線処理、 あるいは水蒸気処理を 行う場合は、 予め上記の範囲の p Hに調整した溶液を茎葉へ噴霧するなどの 処理を行ってから、 加熱処理をすればよい。  In particular, in the case of blanching treatment, it is preferable to use the hot water having the above pH from the viewpoint of obtaining a processed product of sweet potato foliage having a high active ingredient. When performing dry heat treatment, microwave treatment, infrared or far-infrared treatment, or steam treatment, perform treatment such as spraying a solution adjusted to the above-mentioned pH to foliage in advance, and then heat treatment. do it.
p Hの調整は、 当業者が通常用いる方法で行われ得る。 例えば、 塩基性に 調整する場合は、 p H調整剤として、 水酸化ナトリウム、 重曹、 炭酸カルシ ゥム (卵殻カルシウム、 ホタテ貝殻カルシウム、 サンゴカルシウムなど) 、 これらの炭酸力ルシゥムを焼成して得られる酸化カルシウムなどを用い得る。 また、 所定の p Hに調整した水溶液として、 アルカリイオン水などを用いて もよい。 酸性に調整する場合は、 酢酸、 クェン酸、 ァスコルビン酸、 酒石酸、 リンゴ酸、 フマル酸などの有機酸が用いられ得る。 これらの p H調整剤の量 は、 用いる調整剤によって適宜調整される。  Adjustment of pH can be performed by a method commonly used by those skilled in the art. For example, when adjusting to basic, sodium hydroxide, baking soda, calcium carbonate (eg, eggshell calcium, scallop shell calcium, coral calcium, etc.) as a pH adjuster, and calcined calcium carbonate are obtained. Calcium oxide or the like may be used. Further, as the aqueous solution adjusted to a predetermined pH, alkali ion water or the like may be used. When adjusted to be acidic, organic acids such as acetic acid, cunic acid, ascorbic acid, tartaric acid, malic acid, and fumaric acid can be used. The amounts of these pH regulators are appropriately adjusted depending on the regulator used.
さらに、 得られる甘藷茎葉加工物の風味を改善する観点から、 上記 p H調 整された熱水または溶液に 0 . 0 1〜 5 . 0質量%、 好ましくは 0 . 2〜 3 . 0質量%の食塩を添加することが好ましい。 このように食塩を添加すること により、 さらに緑色が鮮やかで、 かつ風味がよい甘藷茎葉加工物を得ること ができる。 Further, from the viewpoint of improving the flavor of the obtained processed sweet potato foliage, the pH It is preferable to add 0.1 to 5.0% by mass, preferably 0.2 to 3.0% by mass of sodium chloride to the prepared hot water or solution. By adding salt in this way, it is possible to obtain a sweet and foliage-processed sweet potato with a brighter green color and a better flavor.
加熱温度については、 8 0 °Cより高い温度、 好ましくは 9 0 °C以上の温度 で行う。 また、 加熱時間は、 5分未満、 好ましくは 3分以下、 最も好ましく は 1 0秒〜 3分である。  The heating temperature is higher than 80 ° C, preferably 90 ° C or higher. The heating time is less than 5 minutes, preferably 3 minutes or less, and most preferably 10 seconds to 3 minutes.
加熱処理後、 甘藷茎葉の緑色および風味を維持する上で、 直ちに冷却する ことが好ましい。 冷却は、 冷水中への浸漬、 冷風による急冷など、 当業者が 通常用いる手段で行われる。 冷却の温度が低いほど、 甘藷茎葉の緑色が映え るようになり、 見た目に美しい。 例えば、 冷水への浸漬処理は、 3 0 °C以下 の水、 好ましくは 2 0 °C以下の水を用いて行われる。 冷却時間は、 甘藷茎葉 の処理量に応じた任意の時間であるが、 甘藷茎葉自身が冷却温度と同等にな るまで行うことが好ましい。  After the heat treatment, it is preferable to immediately cool the sweet potato stems and leaves in order to maintain the green color and flavor. Cooling is performed by means commonly used by those skilled in the art, such as immersion in cold water or rapid cooling with cold air. The lower the cooling temperature, the greener the sweet potato stems and leaves, and the more beautiful it looks. For example, the immersion treatment in cold water is performed using water at 30 ° C or less, preferably water at 20 ° C or less. The cooling time is an arbitrary time according to the processing amount of the sweet potato foliage, but it is preferably performed until the sweet potato foliage itself becomes equal to the cooling temperature.
( 2 - 3 :固液分離処理) 固液分離処理は、 例えば、 甘藷茎葉を圧搾機などを用いて圧搾する、 ある いは甘藷茎葉の破碎物を濾過するなどの方法によって行われる。 このように して得られる甘藷茎葉の搾汁は、 保存の観点から、 8 0 °C〜1 3 0 °Cで加熱 殺菌することが好ましい。 あるいはさらに、 エキス末などに調製する場合は 減圧濃縮機などを用いて濃縮することが好ましい。  (2-3: Solid-Liquid Separation Treatment) The solid-liquid separation treatment is performed by, for example, squeezing sweet potato stems and leaves using a pressing machine or filtering crushed sweet potato stems and leaves. From the viewpoint of preservation, the sweet potato squeezed leaves thus obtained are preferably sterilized by heating at 80 ° C to 130 ° C. Alternatively, when it is prepared into an extract powder or the like, it is preferable to concentrate using a vacuum concentrator.
( 2 - 4 :抽出) 抽出は、 例えば、 刈り取りをした甘藷茎葉の生葉、 あるいは甘藷茎葉の乾 燥物.(粉末) などに、 溶媒を加え、 所定の温度で保持することによって行わ れる。 抽出溶媒としては、 例えば、 水、 有機溶媒、 含水有機溶媒などが挙げ られる。  (2-4: Extraction) The extraction is carried out by adding a solvent to the cut fresh leaves of sweet potato stems or leaves (powder) or the like and keeping the mixture at a predetermined temperature. Examples of the extraction solvent include water, an organic solvent, a water-containing organic solvent, and the like.
上記水としては、 例えば、 湧水、 井戸水、 蒸留水、 水道水、 純水などが用 いられる。 抽出効率の観点から、 熱水を用いることが好ましい。 Examples of the water include spring water, well water, distilled water, tap water, pure water, etc. You can. From the viewpoint of extraction efficiency, it is preferable to use hot water.
上記有機溶媒としては、 例えば、 メタノール、 エタノール、 1 _プロパノ ール、 2—プロパノーノレ、 1—ブタノール、 2—ブタノール、 アセトン、 へ キサン、 シク口へキサン、 プロピレングリコーノレ、 ェチルメチルケトン、 グ リセリン、 酢酸メチル、 酢酸ェチル、 ジェチルエーテル、 ジクロロメタン、 食用油脂、 1 , 1 , 1 , 2—テトラフルォロェタン、 および 1 , 1 , 2—ト リクロロェタンが挙げられる。 好ましくはメタノール、 エタノールなどの極 性有機溶媒である。  Examples of the organic solvent include methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, acetone, hexane, cyclohexane, propylene glycolone, ethyl methyl ketone, Lyserin, methyl acetate, ethyl acetate, getyl ether, dichloromethane, edible fats, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethane. Preferred are polar organic solvents such as methanol and ethanol.
上記含水有機溶媒は、 有効成分を効率的に抽出する点から、 好ましく用い られる。 好ましくは含水極性有機溶媒、 より好ましくは含水アルコール、 さ らに好ましくは含水エタノールである。 含水有機溶媒中の水の含有量は、 例 えば、 1 0容量%以上、 好ましくは 2 0容量%以上、 より好ましくは 5 0容 量%以上、 さらに好ましくは 8 0容量%以上である。 特に、 甘藷茎葉中の有 効成分であるトリカフヱオイルキナ酸を効率よく抽出したい場合は、 ェタノ —ルを 2 0容量%〜9 0容量%含有する含水エタノールが用いられる。  The water-containing organic solvent is preferably used from the viewpoint of efficiently extracting the active ingredient. Preferably, it is a water-containing polar organic solvent, more preferably a water-containing alcohol, and further preferably a water-containing ethanol. The content of water in the water-containing organic solvent is, for example, 10% by volume or more, preferably 20% by volume or more, more preferably 50% by volume or more, and further preferably 80% by volume or more. In particular, when it is desired to efficiently extract tricaffoil quinic acid, which is an active ingredient in sweet potato leaves, hydrous ethanol containing 20% to 90% by volume of ethanol is used.
抽出溶媒の量は特に制限されない。 通常、 甘藷茎葉 1質量部に対して、 抽 出溶媒を好ましくは 0 . 5質量部〜 5 0質量部加えられる。  The amount of the extraction solvent is not particularly limited. Usually, 0.5 to 50 parts by mass of the extraction solvent is added to 1 part by mass of sweet potato leaves.
抽出温度は、 抽出溶媒の種類などに応じて適宜設定すればよく、 例えば、 4 °C以上、 1 0 °C以上、 2 5 °C以上、 5 0 °C以上、 7 0 °C以上、 9 0 °C以上、 9 5 °C以上、 1 0 0 °C以上、 1 2 1 °C以上、 および 1 3 0 °C以上に設定され 得る。 甘藷茎葉中の有効成分の分解を防止する観点から、 低温で行うことが 好ましい。 なお、 抽出溶媒に応じて圧力下で抽出を行ってもよい。  The extraction temperature may be appropriately set according to the type of the extraction solvent, and may be, for example, 4 ° C or more, 10 ° C or more, 25 ° C or more, 50 ° C or more, 70 ° C or more, 9 ° C or more. It can be set to 0 ° C or higher, 95 ° C or higher, 100 ° C or higher, 121 ° C or higher, and 130 ° C or higher. From the viewpoint of preventing the decomposition of the active ingredient in the sweet potato leaves, it is preferable to carry out the reaction at a low temperature. The extraction may be performed under pressure depending on the extraction solvent.
抽出時間は、 抽出温度などに応じて適宜設定される。 例えば、 5分以上、 好ましくは 1 0分以上、 より好ましくは 3 0分以上、 あるいは 4 8時間以下、 好ましくは 2 4時間以下の範囲で適宜設定され得る。  The extraction time is appropriately set according to the extraction temperature and the like. For example, it can be appropriately set within a range of 5 minutes or more, preferably 10 minutes or more, more preferably 30 minutes or more, or 48 hours or less, preferably 24 hours or less.
なお、 短時間抽出を行う場合、 例えば、 抽出時間を 3 0分以下とする場合 は、 甘藷茎葉の乾燥粉末またはエキス末を用いて抽出することが好ましい。 甘藷茎葉をそのまま用いると、 甘藷茎葉中のポリフエノールが充分抽出され ない場合があるからである。 When extracting for a short time, for example, when the extraction time is set to 30 minutes or less Is preferably extracted using a dry powder or extract powder of sweet potato foliage. If sweet potato stems and leaves are used as they are, polyphenol in the sweet potato stems and leaves may not be sufficiently extracted.
具体的には、 甘藷茎葉 1質量部に対して、 水もしくはエタノール含有量が 1◦〜 9 0容量%の含水エタノールを 0 . 5質量部〜 5 0質量部加えて、 1 0 °C〜1 0 0 °Cにて 1 0分〜 4 8時間抽出することによって、 甘藷茎葉の抽 出物を得ることができる。  Specifically, 0.5 parts by mass to 50 parts by mass of water-containing ethanol having a water or ethanol content of 1 ° to 90% by volume is added to 1 part by mass of sweet potato stems and leaves. Extraction of sweet potato stems and leaves can be obtained by extracting at 00 ° C for 10 minutes to 48 hours.
また、 別の抽出方法として、 合成吸着剤 (例えばダイアイオン H P 2 0や セファビース S P 8 2 5、 アンバーライト X AD 4、 MC I g e 1 C H P 2 O P、 クロマトレックス O D S ) ゃデキストラン樹脂 (例えばセフアデック ス L H— 2 0 ) などを用いる、 当業者が通常用いる天然のポリフエノールの 分離方法を採用してもよい。 この場合、 特に、 抽出用溶媒として、 水または 含水極性溶媒 (含水アルコール、 特に含水エタノール等) を用いることが、 ポリフエノールを効率よく抽出できる点で好ましい。 この分離方法は、 上記 で得られる甘藷茎葉の抽出物をさらに精製する目的においても、 必要に応じ て採用され得る。  As another extraction method, synthetic adsorbents (eg, Diaion HP20, Sephavis SP825, Amberlite XAD4, MCImage 1 CHP2OP, Chromatorex ODS) ゃ Dextran resin (eg, Sephadex) For example, a natural polyphenol separation method commonly used by those skilled in the art using LH-20) may be employed. In this case, it is particularly preferable to use water or a water-containing polar solvent (a water-containing alcohol, particularly water-containing ethanol, etc.) as the extraction solvent, since polyphenol can be efficiently extracted. This separation method can be adopted as needed for the purpose of further purifying the sweet potato stem and leaf extract obtained above.
( 2 - 5 :乾燥および粉碎処理)  (2-5: Drying and grinding)
上記乾燥および粉枠処理は、 甘藷茎葉の乾燥物、 乾燥粉末、 乾燥エキス末 を得る目的で行われる。  The drying and the powder frame treatment are performed for the purpose of obtaining dried sweet potato stems and leaves, dried powder, and dried extract powder.
乾燥は、 長期保存の目的、 品質の劣化防止や商品価値を高める目的などで 行われる。 乾燥方法は、 特に制限されないが、 例えば、 熱風乾 «、 高圧蒸 気乾燥機、 電磁波乾燥機、 凍結乾燥機、 直火式加熱機、 回転式乾燥機などを 用いた方法が挙げられる。 製造上のコストや乾燥の効率の面からは、 熱風乾 Drying is performed for the purpose of long-term storage, prevention of deterioration of quality and enhancement of commercial value. The drying method is not particularly limited, and examples thereof include a method using a hot air dryer, a high-pressure steam dryer, an electromagnetic wave dryer, a freeze dryer, a direct fire heater, a rotary dryer and the like. In terms of manufacturing costs and drying efficiency, hot air drying
• m, 直火式加熱機、 回転式通風乾燥機を用いることが好ましい。 甘藷茎葉 の搾汁などの液状物を乾燥する場合は、 減圧濃縮、 噴霧乾燥などを行うこと も可能である。 製造上のコストや乾燥の効率の面から、 減圧濃縮および噴霧 乾燥が好ましい。 乾燥温度は、 常圧下においては、 6 0 °C〜1 5 0 °C程度で 行うことが、 風味が良く、 色鮮やかな甘藷茎葉の乾燥粉末が得られる点で好 ましい。 減圧下においては、 好ましくは 6 0 °C以下で、 かつ甘藷茎葉、 ぺー スト、 または搾汁が凍結しない圧力下で行えば、 栄養成分の損失を少なくし つつ乾燥することができる。 乾燥は、 通常、 乾燥物またはエキス末中の水分 含量が 5質量%以下となることを目安に行われる。 • It is preferable to use m, direct-fired heaters and rotary ventilation dryers. When drying liquids such as sweet potato squeezed leaves, concentration under reduced pressure and spray drying can be performed. Vacuum concentration and spraying in terms of manufacturing cost and drying efficiency Drying is preferred. The drying temperature is preferably about 60 ° C. to 150 ° C. under normal pressure, because a flavorful and colorful dried powder of sweet potato stems and leaves can be obtained. If the drying is performed under reduced pressure, preferably at a temperature of 60 ° C. or lower, and at a pressure at which the sweet potato stems and leaves, paste, or squeezed juice does not freeze, drying can be performed while reducing loss of nutrient components. Drying is usually performed on the basis that the moisture content in the dried product or extract powder is 5% by mass or less.
例えば、 熱風乾燥機などを用いて乾燥する場合、 甘藷茎葉をそのまま乾燥 してもよいが、 2段階乾燥を行うことが好ましい。 この 2段階の乾燥工程を 行うことにより、 乾燥時間が短縮されると同時に、 甘藷茎葉の緑色および風 味が維持される。 2段階乾燥は、 例えば、 甘藷茎葉または甘藷茎葉の加工物 を、 まず、 水分含有量が 2 5質量%以下となるまで、 6 0〜8 0 °Cの温度で —次乾燥し、 次いで、 一次乾燥した甘藷茎葉の水分含量が 5質量%以下とな るまで、 一次乾燥よりも高い温度で、 かつ 1 0 0 °C以下、 好ましくは 9 0 °C 以下、 より好ましくは 8 5 °C以下、 さらに好ましくは 8 0 °C前後の温度で二 次乾燥することによつて行われる。 二次乾燥温度を 1 0 0 °C以下に設定する ことによって、 ポリフエノール含有量が多く色鮮やかな甘藷茎葉粉末を得る ことができる。 一次乾燥温度が 6 0 °C未満では、 乾燥速度が遅くなる場合が あり、 二次乾燥温度が 1 0 0 °Cを超える場合は、 焦げを生じることがある。 一次乾燥温度および二次乾燥温度が上記範囲を満たす場合、 さらに一次乾燥 と二次乾燥との温度差が、 約 5〜 1 5 °Cであることが好ましく、 約 1 0 で あることがより好ましい。 温度差を上記のように一定範囲に設定することに より、 乾燥工程における甘藷茎葉の水分管理が容易になり、 効率的に乾燥を 行うことができる。  For example, when drying using a hot air dryer or the like, the sweet potato foliage may be dried as it is, but it is preferable to perform two-stage drying. By performing the two-stage drying process, the drying time is shortened, and at the same time, the green color and flavor of the sweet potato stems and leaves are maintained. The two-stage drying is performed, for example, by first drying sweet potato stems or leaves at a temperature of 60 to 80 ° C. until the water content becomes 25% by mass or less, followed by primary drying. Until the moisture content of the dried sweet potato foliage is 5% by mass or less, at a temperature higher than the primary drying, and 100 ° C or less, preferably 90 ° C or less, more preferably 85 ° C or less, More preferably, secondary drying is performed at a temperature of about 80 ° C. By setting the secondary drying temperature to 100 ° C. or less, a colorful sweet potato stem and leaf powder having a large polyphenol content can be obtained. If the primary drying temperature is lower than 60 ° C, the drying speed may be slow. If the secondary drying temperature is higher than 100 ° C, scorching may occur. When the primary drying temperature and the secondary drying temperature satisfy the above ranges, the temperature difference between the primary drying and the secondary drying is preferably about 5 to 15 ° C, more preferably about 10 ° C. . By setting the temperature difference within a certain range as described above, the moisture management of the sweet potato stems and leaves in the drying step becomes easy, and drying can be performed efficiently.
上記噴霧乾燥は、 甘藷茎葉の搾汁などをエキス末とする場合にスプレード ライヤ一などの噴霧乾燥機を用いて行われる。 嘖霧乾燥を用いる場合は、 回 収率を上げるために、 必要に応じてデキストリン、 シクロデキストリン、 デ ンプン、 マルトースのような賦形剤を添カ卩して行われる。 好適にはデキスト リンが用いられ、 搾汁とデキストリンの比は、 デキストリン添カ卩により粉末 化を容易にするため、 質量比で 1 : 1 0〜5 : 1が好ましい。 The above spray drying is performed using a spray dryer such as a spray dryer when extracting the juice of sweet potato stems and leaves into an extract powder. When using simulant drying, dextrin, cyclodextrin, This is done by adding excipients such as starch and maltose. Dextrin is preferably used, and the ratio of squeezed juice to dextrin is preferably 1:10 to 5: 1 by mass in order to facilitate pulverization with dextrin-added mash.
上記乾燥によって、 例えば、 甘藷茎葉、 甘藷茎葉の加熱処理物などからは、 甘藷茎葉の乾燥物 (乾燥粉末) が得られ、 甘藷の搾汁からは、 甘藷茎葉の乾 燥エキス末が得られる。 特に甘藷茎葉の加熱処理物を乾燥して得られる甘藷 茎葉の乾燥物が甘藷加工物として好適に用いられる。 なお、 これらの乾燥物 (特にエキス末) は、 目的に応じて適宜殺菌して用いられる。  By the above-mentioned drying, for example, a dried product (dry powder) of sweet potato stem and leaves is obtained from sweet potato stems and leaves and a heat-treated product of sweet potato stems and leaves, and a dried extract powder of sweet potato stem and leaves is obtained from sweet potato juice. In particular, dried sweet potato stems and leaves obtained by drying the heat-treated sweet potato stems and leaves are suitably used as processed sweet potatoes. These dried products (especially extract powder) are used after appropriately sterilizing according to the purpose.
粉砕処理は、 甘藷茎葉の乾燥物を粉末化する目的で、 あるいは乾燥粉末を さらに微粉ィ匕する目的で行われる。 粉砕することによって、 更に粒径を小さ く、 均一な微粉末を得ることができる。 甘藷茎葉には、 硬さが異なる茎部、 葉部および葉柄部が含まれるため、 粉碎の効率を上げる観点からは、 粗粉碎 工程および微粉碎工程を経ることが好ましい。  The crushing treatment is performed for the purpose of pulverizing the dried product of the sweet potato stem or leaves, or for further pulverizing the dried powder. By pulverization, a uniform fine powder having a smaller particle size can be obtained. Since sweet potato stems include stems, leaves, and petiole parts having different hardnesses, it is preferable to go through a coarse pulverizing step and a fine pulverizing step from the viewpoint of increasing the efficiency of pulverization.
粗粉石争は、 例えば、 甘藷茎葉の乾燥物をカッター、 スライサー、 ダイサー などの当業者に公知の任意の機械または道具を用いてカットされる。 カット された甘藷茎葉の乾燥物の大きさは、 長径が 2 O mm以下であり、 好ましく は 0 . 1〜1 0 mmである。  The crushed stones are cut, for example, from dried sweet potato foliage using any machine or tool known to those skilled in the art, such as a cutter, slicer, dicer and the like. The size of the dried dried sweet potato stem and leaf has a major axis of 20 mm or less, preferably 0.1 to 10 mm.
続いて、 粗粉碎した甘藷茎葉の乾燥物を、 微粉碎するが、 微粉砕工程の前 に殺菌してもよレ、。 殺菌を施すことにより、 粗粉碎した甘藷茎葉を均一に加 熱することができ、 緑葉の香味を良好にしつつ、 効率の良い殺菌を行うこと ができる。 殺菌は、 高圧殺菌機、 加熱殺菌機、 加圧蒸気殺菌機などを用いて 1 1 o °c以上で行われる。 例えば、 加圧蒸気殺菌による加熱処理の場合、 粗 粉砕した甘藷茎葉加工物は、 例えば、 0. 5〜: L 0 k g / c m2の加圧下、 1 1 0〜 2 0 0 °Cの飽和水蒸気により、 2〜 1 0秒間加熱処理される。 必要 に応じて、 飽和蒸気による加熱時に含んだ水分をさらに乾燥する。 Subsequently, the coarsely ground dried sweet potato stems and leaves are finely ground, but may be sterilized before the fine grinding step. By sterilizing, coarsely ground sweet potato stems and leaves can be uniformly heated, and efficient sterilization can be performed while improving the flavor of green leaves. Sterilization is performed at 11 o ° C or higher using a high-pressure sterilizer, a heat sterilizer, a pressurized steam sterilizer, or the like. For example, in the case of heat treatment by pressurized steam sterilization, coarsely pulverized sweet potato stem and leaf products are, for example, 0.5 to: L 0 kg / cm 2 under a pressure of 110 to 200 ° C saturated steam. For 2 to 10 seconds. If necessary, further dry the water contained during heating with saturated steam.
微粉碎は、 好ましくは 9 0質量%が 2 0 0メッシュ区分を通過するように 粉砕される。 微粉砕は、 例えば、 クラッシャー、 ミル、 プレンダー、 石臼な どの当業者が通常用いる任意の機械または道具を用いて行えば良い。 微粉碎 することにより嗜好性がよくなるが、 好ましくは、 粗粉砗、 加熱、 およぴ微 粉砕の工程を順に経ることにより、 さらに嗜好性がよくなる。 Pulverization is preferably performed so that 90% by mass passes through the 200 mesh section. Crushed. The pulverization may be performed using any machine or tool usually used by those skilled in the art, such as a crusher, a mill, a blender, and a stone mill. The pulverization improves the palatability, but preferably the pulverization, heating, and pulverization steps in this order further enhance the palatability.
( 3 ) 第 1の組成物の調製および用途  (3) Preparation and use of the first composition
本発明の第 1の組成物は、 上記甘藷茎葉の加工物を含有し、 該甘藷茎葉中 に若茎葉が含まれる。 上記甘藷茎葉の加工物の含有量は特に制限されないが、 好ましくは 0 . 0 0 0 1質量%以上である。 本発明の第 1の組成物は、 食品、 医薬品、 医薬部外品、 化粧品、 皮膚外用剤などとして利用され得る。 上記甘 藷茎葉の加工物は、 そのまま第 1の組成物として利用することも可能であり、 例えば、 甘藷茎葉ゃ甘藷茎葉の乾燥粉末を熱水抽出して得られる抽出エキス は、 そのままお茶として利用することができる。  The first composition of the present invention contains the processed product of the above-mentioned sweet potato stem and leaves, and the young shoot and leaf are contained in the sweet potato stem and leaves. The content of the processed product of the sweet potato foliage is not particularly limited, but is preferably 0.0001% by mass or more. The first composition of the present invention can be used as foods, pharmaceuticals, quasi-drugs, cosmetics, skin external preparations, and the like. The processed product of the sweet potato stems and leaves can be used as it is as the first composition.For example, an extract obtained by hot-water extraction of a dry powder of sweet potato stems and leaves / sweet potato stems and leaves is directly used as tea. can do.
本発明の第 1の組成物は、 甘藷茎葉の加工物以外に、 特に糖アルコールお よび二糖以上の糖からなる群より選択される少なくとも 1種の糖類を含有す ることが好ましい。 本発明においては、 甘藷茎葉の加工物と、 上記糖類との 組み合わせの詳細については、 特に第 2の組成物として後述する。  The first composition of the present invention preferably contains at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more, in addition to processed sweet potato stems and leaves. In the present invention, the details of the combination of the processed product of the sweet potato stem and leaves and the above-mentioned saccharide will be specifically described later as the second composition.
本発明の第 1の組成物は、 さらに必要に応じて、 賦形剤、 増量剤、 結合剤、 増粘剤、 乳化剤、 着色料、 香料、 食品添加物、 調味料、 医薬品原料などのそ の他の成分が適宜含有され得る。  The first composition of the present invention may further contain, if necessary, excipients, extenders, binders, thickeners, emulsifiers, coloring agents, flavors, food additives, seasonings, raw materials for pharmaceuticals, and the like. Other components may be included as appropriate.
上記食品添加物としては、 例えば、 ローヤルゼリー、 プロポリス、 チォク ト酸およびその誘導体、 ビタミン類 (A、 B群、 C、 D、 E、 K、 葉酸、 ノ ントテン酸、 ピオチン、 これらの誘導体等) 、 ミネラル (鉄、 マグネシウム、 カルシウム、 亜鉛等) 、 セレン、 キチン'キトサン、 レシチン、 ポリフエノ ール (カテキン類、 アントシァニン類、 プロアントシァニジンなどの縮合型 タンニン、 ガロタンニン等の加水分解型タンニン、 フラボノイド類、 これら の誘導体等) 、 カロテノイド (リコピン、 ァスタキサンチン、 ゼアキサンチ ン、 ルティン等) 、 サポニン (イソフラボン、 ジンセサノィド、 グリチルリ チン酸等) 、 キサンチン誘導体 (カフェイン等) 、 脂肪酸、 アミノ酸、 タン パク質 (コラーゲン、 エラスチン等) 、 ムコ多糖類 (ヒアルロン酸、 コンド ロイチン、 デルマタン、 へパラン、 へパリン、 ケタラン、 これらの塩等) 、 アミノ糖 (ダルコサミン、 ァセチルダルコサミン、 ガラクトサミン、 ァセチ ルガラタトサミン、 ノィラミン酸、 ァセチルノイラミン酸、 へキソサミン、 それらの塩等) 、 食物繊維 (アルギン酸、 グァガム、 ぺクチン、 ダルコマン ナン等) 、 リン脂質及びその誘導体 (フォスファチジルコリン、 フォスファ チジルセリン等) 、 スフインゴ脂質およびその誘導体 (スフインゴミエリン、 セラミ ド等) 、 含硫化合物 (ァリイン、 セパェン、 タウリン、 ダルタチオン、 メチルスルホニルメタン等) 、 キノン類 (コェンザィム Q 1 0等) 、 リグナ ン類 (セサミン等) 、 これらを含有する動植物抽出物、 根菜類 (ゥコン、 シ ヨウガ等) 、 麦若葉末等のイネ科植物の緑葉、 ケール等のアブラナ科植物の 緑葉などが挙げられる。 Examples of the above food additives include royal jelly, propolis, lactic acid and derivatives thereof, vitamins (A, B, C, D, E, K, folic acid, nontothenic acid, biotin, and derivatives thereof), Minerals (iron, magnesium, calcium, zinc, etc.), selenium, chitin 'chitosan, lecithin, polyphenols (condensed tannins such as catechins, anthocyanins, proanthocyanidins, hydrolyzed tannins such as gallotannins, flavonoids) , Their derivatives, etc.), carotenoids (lycopene, astaxanthin, zeaxanthi) , Saponins (isoflavones, ginsesanoides, glycyrrhizic acid, etc.), xanthine derivatives (caffeine, etc.), fatty acids, amino acids, proteins (collagen, elastin, etc.), mucopolysaccharides (hyaluronic acid, chondroitin, etc.) Dermatan, heparan, heparin, ketalan, their salts, etc.), amino sugars (darcosamine, acetyldarcosamine, galactosamine, acetyl gargalatasamine, neuraminic acid, acetylneuraminic acid, hexosamine, salts thereof, etc.), foods Fibers (alginic acid, guar gum, pectin, dalcomannan, etc.), phospholipids and their derivatives (phosphatidylcholine, phosphatidylserine, etc.), sphingolipids and their derivatives (sphingomyelin, ceramide, etc.), Sulfur compounds (aliin, sephaen, taurine, daltathione, methylsulfonylmethane, etc.), quinones (koenzam Q10, etc.), lignans (sesamin, etc.), animal and plant extracts containing these, root vegetables (ゥ kon, shiyoga) And the like, green leaves of grasses such as wheat young leaf powder, and green leaves of cruciferous plants such as kale.
上記食品添加物の中でも優れた抗糖尿病効果が得られる点で、 特にビタミ ン B群、 α—リポ酸などの糖の代謝促進成分、 グアバ葉などの糖の消化吸収 抑制成分、 およびこれらの成分を含有する食品原料;キトサン、 サイリゥム などの脂質吸収抑制成分、 L一カルニチン、 C o Q l 0などの脂質代謝促進 成分、 およびこれらの成分を含有する食品原料が好ましく用いられる。 これ らの成分を含有することによって得られる優れた抗糖尿病効果は、 (1 ) 甘 藷茎葉によるィンスリンの分泌促進作用およびィンスリンの感受性増強作用 と、 (2 ) 糖の代謝促進成分または糖の消化吸収抑制成分を有する原料とに よる相乗的な効果、 あるいは (1 ) および (2 ) の効果と、 脂質吸収抑制成 分または脂質代謝促進成分を有する原料とによる相乗的な効果であると考え られ、 本発明の第 1の組成物は、 糖および脂質の吸収抑制成分やそれらの代 謝促進成分を単に組合せた場合に比べて優れた抗糖尿病効果を示す。 本発明の第 1の組成物中の上記糖の代謝促進成分、 糖の消化吸収抑制成分、 脂質吸収抑制成分、 または脂質代謝促進成分の含有量は特に限定されない。 好ましくは甘藷茎葉の加工物 1 0 0質量部に対し、 糖の消化吸収抑制成分、 脂質吸収抑制成分、 および脂質代謝促進成分の合計量が、 1質量部〜 5 0 0 0質量部、 好ましくは 5質量部〜 2 5 0 0質量部となるように含有される。 さらに、 上記食品添加物を含む飲科、 例えば、 植物発酵ジュース、 野菜ジ ユース (例えば、 人参ジュース) 、 植物抽出物、 果汁なども利用され得、 こ れらを含有させることにより、 機能性または栄養価の高い飲料を得ることが できる。 そしてこれらは、 調味料等を加えて、 味を整えることができる。 本発明の第 1の組成物の形態については特に制限されない。 液体の場合は、 そのまま飲料として用いることができる。 また必要に応じて、 ハードカプセ ル、 ソフトカプセルなどのカプセル剤、 錠剤、 もしくは丸剤などに、 あるい は粉末状、 顆粒状、 茶状、 ティーバッグ状、 もしくは飴状などの形態に成形 することができる。 本発明の第 1の組成物は、 これらの形状または好みに応 じて、 そのまま食してもよく、 あるいは水、 湯、 牛乳などに溶いて飲んでも 良い。 また、 粉末ィ匕してティーバッグ状などの場合、 成分を浸出させてから 飲んでも良い。 Among the above-mentioned food additives, excellent antidiabetic effects can be obtained. Particularly, vitamin B group, a component for promoting metabolism of sugar such as α-lipoic acid, a component for suppressing digestion and absorption of sugar such as guava leaf, and these components Food ingredients containing these; lipid absorption suppressing ingredients such as chitosan and thyrimu; lipid metabolism promoting ingredients such as L-carnitine and CoQ10; and food ingredients containing these ingredients are preferably used. The excellent anti-diabetic effects obtained by containing these components are (1) the action of enhancing the secretion of insulin and the sensitivity of insulin by the stems and leaves of sweet potatoes, and (2) the component of promoting sugar metabolism or digestion of sugar. It is considered that the synergistic effect of the raw material having the absorption-inhibiting component or the synergistic effect of (1) and (2) and the raw material having the lipid absorption-inhibiting component or the lipid metabolism-promoting component is considered. On the other hand, the first composition of the present invention exhibits an excellent antidiabetic effect as compared with a case where a sugar and lipid absorption-inhibiting component or a metabolic promoting component thereof is simply combined. The content of the sugar metabolism promoting component, the sugar digestion and absorption suppressing component, the lipid absorption suppressing component, or the lipid metabolism promoting component in the first composition of the present invention is not particularly limited. Preferably, the total amount of the sugar digestion and absorption inhibitory component, the lipid absorption inhibitory component, and the lipid metabolism promoting component is 1 part by mass to 500 parts by mass, preferably 100 parts by mass of the processed product of the sweet potato stem and leaves. It is contained so as to be 5 to 250 parts by mass. In addition, beverages containing the above food additives, for example, fermented vegetable juice, vegetable juice (eg, ginseng juice), plant extract, fruit juice, etc., can be used. A nutritious beverage can be obtained. These can be seasoned by adding seasonings and the like. The form of the first composition of the present invention is not particularly limited. In the case of a liquid, it can be used as a beverage as it is. If necessary, capsules such as hard capsules and soft capsules, tablets, or pills, or powders, granules, tea, tea bags, or candy can be formed. it can. The first composition of the present invention may be eaten as it is, or may be dissolved in water, hot water, milk, or the like, depending on its shape or preference. In the case of a powdered tea bag or the like, the ingredients may be leached before drinking.
本発明の第 1の組成物を皮膚外用剤 (化粧品、 トイレタリー用品など) と して利用する場合は、 種々の基材を添カ卩して、 例えば、 化粧水、 化粧タリー ム、 乳液、 パック、 ヘアトニック、 シャンプー、 ヘアリンス、 トリートメン ト、 ボディーシャンプー、 先顔剤、 石鹼、 ファンデーション、 口紅、 育毛剤、 軟膏、 入浴剤、 歯磨剤、 マウスゥォッシュ、 シップ、 ゲルなどの形態とされ 得る。 ( 4 ) 第 2の組成物  When the first composition of the present invention is used as an external preparation for the skin (cosmetics, toiletries, etc.), various base materials are added to the composition, for example, a lotion, a cosmetic toilet, a milky lotion, and a pack. , Hair tonics, shampoos, hair rinses, treatments, body shampoos, facial agents, stones, foundations, lipsticks, hair restorers, ointments, bath salts, dentifrices, mouthwashes, ships, gels and the like. (4) Second composition
本発明の第 2の組成物は、 甘藷茎葉の加工物と、 糖アルコールおよび二糖 以上の糖からなる群より選択される少なくとも 1種の糖類とを含有すること を特 ί敷とする。 A second composition of the present invention comprises a processed product of sweet potato stem and leaves, a sugar alcohol and a disaccharide. It is characterized in that it contains at least one saccharide selected from the group consisting of the above saccharides.
( 4 - 1 ) 甘藷茎葉およびその加工物  (4-1) Sweet potato stems and leaves and processed products
本発明の第 2の組成物に用いられる甘藷茎葉としては、 若茎葉を含まない 甘藷茎葉も用いることができるが、 若茎葉を含む甘藷茎葉を用いてもよレ、。 より機能性の高い組成物とする観点からは、 若茎葉を含む甘藷茎葉を用いる ことが好ましい。 若茎葉を含まない甘藷茎葉の加工物の調製方法は、 上記と 同じである。  As the sweet potato stem and leaves used in the second composition of the present invention, a sweet potato stem and leaf that does not include a young stem leaf can be used, but a sweet potato stem and leaf that includes a young stem leaf may be used. From the viewpoint of obtaining a more functional composition, it is preferable to use sweet potato stems and leaves including young stems and leaves. The method for preparing a processed product of sweet potato stems and leaves that does not contain young shoots is the same as described above.
( 4 - 2 ) 糠アルコールまたは二糖以上の糖  (4-2) Bran alcohol or disaccharide or higher sugar
本発明の第 2の組成物には、 糖アルコールまたは二糖以上の糖が含有され る。 糖アルコールおよぴニ糖以上の糖は、 単独であるいは 2以上を組み合わ せて用いられる。  The second composition of the present invention contains a sugar alcohol or a disaccharide or higher sugar. Sugar alcohols and disaccharide or higher sugars are used alone or in combination of two or more.
本明細書において、 「糖アルコール」 とは、 糖分子のカルボ二ル基を還元 して得られる多価アルコールをいう。 糖アルコールとしては、 エリスリ トー ル、 ペンチトール、 へキシトール、 キシリ トール、 ソルビトール、 還元パラ チノース、 マルチトール (還元麦芽糖) 、 ラタチトール、 マンニトールなど が挙げられる。 好ましくはマルチトール (還元麦芽糖) などの水溶性の糖ァ ルコールである。 糖アルコールは、 単独であるいは 2以上を組み合わせて用 いられる。  In the present specification, “sugar alcohol” refers to a polyhydric alcohol obtained by reducing a carboxy group of a sugar molecule. Examples of sugar alcohols include erythritol, pentitol, hexitol, xylitol, sorbitol, reduced palatinose, maltitol (reduced maltose), ratatitol, mannitol and the like. Water-soluble sugar alcohols such as maltitol (reduced maltose) are preferred. Sugar alcohol is used alone or in combination of two or more.
本明細書において、 「二糖以上の糖」 とは、 単糖が 2つ以上結合したもの をいい、 例えば、 二糖、 オリゴ糖、 およぴ多糖類 (例えばデンプンの分解物 等) などが挙げられる。 二糖以上の糖は、 単独であるいは 2以上を組み合わ せて用いられる。  In the present specification, the term “sugar or more sugars” refers to those in which two or more monosaccharides are linked, and examples thereof include disaccharides, oligosaccharides, and polysaccharides (for example, starch decomposed products). No. Disaccharides or more are used alone or in combination of two or more.
二糖としては、 例えば、 トレハロース、 スクロースなどのトレハロース型 二糖 (両構成単糖の還元基をもつ炭素原子がエーテル結合している二糖) 、 マノレトース、 セロビオース、 ゲンチオビオース、 ラタトースなどのマノレトー ス型ニ糖 (第 1の構成単糖の還元基をもつ炭素原子と第 2の単糖の還元基を もたない炭素原子との間でエーテル結合している二糖) などが挙げられる。 オリゴ糖としては、 イソマルトオリゴ糖、 ブラタトオリゴ糖、 ガラクトォ リゴ糖、 パラチノース、 マルトオリゴ糖、 乳果オリゴ糖、 キシロオリゴ糖な どが挙げられる。 Examples of the disaccharide include trehalose-type disaccharides such as trehalose and sucrose (a disaccharide in which a carbon atom having a reducing group of both constituent monosaccharides is an ether bond), and manoletoses such as manoletose, cellobiose, gentiobiose, ratatose, and the like. Disaccharide (a disaccharide in which an ether bond is formed between a carbon atom having a reducing group of the first constituent monosaccharide and a carbon atom having no reducing group of the second monosaccharide), and the like. Oligosaccharides include isomaltooligosaccharides, bratatooligosaccharides, galacto-oligosaccharides, palatinose, maltooligosaccharides, nectar oligosaccharides, xylo-oligosaccharides, and the like.
多糖としては、 1種類の構成糖からなるホモ多糖、 2種類以上の構成糖か らなるヘテロ多糖、 中性多糖、 酸性多糖、 グリコサミノダリカン、 デキスト リン、 デンプン、 アミロース、 アミロぺクチン、 セルロース、 難消化性デキ ストリンなどが挙げられる。 好ましくは難消化性デキストリンである。 上記難消化性デキストリンとは、 デンプンょり得られる難消化性の多糖類 であり、 例えば、 デンプンを加熱により加水分解した後、 アミラーゼにより 加水分解して得られるものであり、 その難消化性成分は、 デンプンが元来有 する、 4結合を主とする。 難消化性デキストリンには、 食後血糖上 昇抑制効果のほか、 血中脂質の改善効果、 整腸効果があると言われている。 難消化性デキストリンは、 粉末、 細粒、 顆粒などの形態で市販されており、 いずれの形態のものも使用可能である。 また、 難消化性デキストリンは水溶 性であるため、 水溶液の形態においても用いられる。  Polysaccharides include homopolysaccharides composed of one kind of constituent sugars, heteropolysaccharides composed of two or more kinds of constituent sugars, neutral polysaccharides, acidic polysaccharides, glycosaminodalican, dextrin, starch, amylose, amylopectin, and cellulose. And indigestible dextrin. Preferred are indigestible dextrins. The indigestible dextrin is an indigestible polysaccharide obtained from starch, and is obtained, for example, by hydrolyzing starch by heating and then hydrolyzing it with amylase. Mainly has four bonds, which starch originally has. Indigestible dextrin is said to have the effect of suppressing postprandial blood glucose elevation, the effect of improving blood lipids, and the effect of intestinal regulation. Indigestible dextrin is commercially available in the form of powder, fine granules, granules, and the like, and any form can be used. Indigestible dextrin is also used in the form of an aqueous solution because it is water-soluble.
上記二糖以上の糖のうちで、 好ましくはオリゴ糖、 デキストリン、 難消化 性デキストリンなどの水溶性の糖であり、 より好ましくは難消化性デキスト リンである。  Among the above-mentioned disaccharides or more, water-soluble sugars such as oligosaccharides, dextrins and indigestible dextrins are preferred, and more preferably indigestible dextrins.
上記二糖類以上の糖は、 構成される糖の種類によって異なるが、 様々な剤 形への応用を可能とする観点から、 平均分子量が 9, 0 0 0以下 (ダルコ一 スに換算すると平均で結合している単糖が 5 0個以下) の糖であることが好 ましい。  The above-mentioned disaccharides and higher saccharides differ depending on the type of saccharides to be composed, but from the viewpoint of application to various dosage forms, the average molecular weight is 9,000 or less (average molecular weight when converted to Darcos). It is preferred that the number of monosaccharides linked is 50 or less).
本発明の第 2の組成物に用いられる二糖以上の糖の構造は、 特に制限はな く、 単糖が結合して環状構造をなしている糖 (例えばシクロデキストリンな ど) や、 単糖が結合して鎖状構造をなしている糖 (麦芽糖、 アミロース、 セ ルロースなど) 、 または、 単糖が結合して枝分かれ状構造をなしている糖 (アミロぺクチン、 ポリデキストロースなど) であればよい。 The structure of the disaccharide or more saccharide used in the second composition of the present invention is not particularly limited, and a saccharide to which a monosaccharide is bonded to form a cyclic structure (for example, cyclodextrin or the like) Etc.), sugars that form a chain structure by combining monosaccharides (maltose, amylose, cellulose, etc.), or sugars that form a branched structure by combining monosaccharides (amylopectin, polysaccharide) Dextrose).
( 4 - 3 ) 第 2の組成物の調製および用途  (4-3) Preparation and use of the second composition
本発明の第 2の組成物は、 甘藷茎葉の加工物と、 糖アルコールおよび二糖 以上の糖からなる群より選択される少なくとも 1種の糖類とを含有する。 本 発明の第 2の組成物は、 食品、 医薬品、 医薬部外品、 化粧品、 皮膚外用剤な ど、 特に食品または医薬品として利用され得る。  The second composition of the present invention contains a processed product of a sweet potato foliage and at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more. The second composition of the present invention can be used as foods, pharmaceuticals, quasi-drugs, cosmetics, external preparations for skin, etc., particularly as foods or pharmaceuticals.
本発明の第 2の組成物中の甘藷茎葉の加工物の含有量は特に制限されない。 好ましくは、 第 1の組成物と同様、 好ましくは 0 . 0 0 0 1質量%以上であ る。 特に甘藷茎葉の加工物が抽出物の乾燥粉末やエキス末の場合は、 好まし くは 0 . 0 0 0 1質量%〜 5 0質量0 /。、 好ましくは 0 . 0 0 1質量。/。〜 3 0 質量%である。 また、 甘藷茎葉をそのまま乾燥粉末にして用いる場合は、 0 . 0 1質量%〜 8 0質量%、 好ましくは 0 . 0 1質量%〜 5 0質量。 /0である。 本発明の第 2の組成物中の糖アルコールおよぴニ糖以上の糖の含有量は、 特に制限されない。 好ましくは甘藷茎葉加工物 1 0 0質量部に対して、 1 0 〜 2 0 0 0質量部、 より好ましくは 1 0〜 1 0 0 0質量部である。 造粒性と 嗜好性の観点からは、 糖アルコールおよぴニ糖以上の糖は、 通常、 組成物中 に、 好ましくは 3質量%以上、 より好ましくは 5質量%以上含有される。 本発明の第 2の組成物は、 上記甘藷茎葉の加工物、 糖アルコール、 および 二糖以上の糖以外に、 必要に応じて、 第 1の組成物で用いたその他の成分が 含有され得る。 The content of the processed product of the foliage of sweet potato in the second composition of the present invention is not particularly limited. Preferably, as in the first composition, it is preferably 0.0001% by mass or more. In particular, when the processed product of the sweet potato stem and leaf is a dry powder of the extract or an extract powder, the content is preferably 0.0001% by mass to 50% by mass / 0 . , Preferably 0.001 mass. /. To 30% by mass. When the sweet potato foliage is used as it is as a dry powder, it is used in an amount of 0.01 to 80% by mass, preferably 0.01 to 50% by mass. / 0 . The content of sugar alcohol and sugars higher than disaccharide in the second composition of the present invention is not particularly limited. Preferably, the amount is 10 to 2000 parts by mass, more preferably 10 to 1000 parts by mass, based on 100 parts by mass of the processed product of sweet potato foliage. From the viewpoints of granulation properties and palatability, sugars such as sugar alcohols and disaccharides are usually contained in the composition preferably in an amount of 3% by mass or more, more preferably 5% by mass or more. The second composition of the present invention may contain, as necessary, other components used in the first composition, in addition to the processed product of the sweet potato stem and leaves, sugar alcohol, and disaccharide or more saccharides.
本発明の第 2の組成物の剤形については特に限定はなく、 第 1の組成物と 同様の形態に成形され得る。 好ましくは、 ペースト、 ゲルおよび固形の造粒 物 (顆粒、 細粒、 錠剤、 混練物、 焼き菓子等) であり、 より好ましくは造粒 物である。 本発明の第 2の組成物は、 造粒性に優れるため、 上述のように、 特に造粒物として好適に用いられる。 造粒方法は、 一般に用いられている造 粒法、 例えば、 転動造粒法、 混合攪拌造粒法、 押し出し造粒法、 流動層造粒 法、 乳化造粒法、 流動造粒、 スプレードライヤーを用いた造粒法、 コーティ ング造粒法などを用いて成形され得る。 The dosage form of the second composition of the present invention is not particularly limited, and can be formed in the same form as the first composition. Preferred are pastes, gels, and solid granules (granules, fine granules, tablets, kneaded materials, baked goods, etc.), and more preferably granules. Since the second composition of the present invention has excellent granulation properties, as described above, Particularly, it is suitably used as a granulated product. Granulation methods include commonly used granulation methods, for example, tumbling granulation method, mixing stirring granulation method, extrusion granulation method, fluidized bed granulation method, emulsion granulation method, fluidized granulation, spray dryer It can be formed using a granulation method using a coating, a coating granulation method, or the like.
本発明の第 2の組成物の摂取量は特に制限されない。 好ましくは、 成人 1 日あたりの摂取量が、 甘藷茎葉の加工物に含有されるジカフヱオイルキナ酸 等の合計量として 1 0 m g〜3 0 0 0 m g、 好ましくは 1 0 m g〜: L 0 0 0 m gとなるように摂取される。 なお、 甘藷茎葉の乾燥粉末の場合は、 0 . 1 g〜3 0 g、 好ましくは 0 . 1〜1 0 gとなるように配合されればよい。 本発明の第 2の組成物は、 優れた造粒性および安定性を示すと共に、 糖ァ ルコールぉよぴニ糖以上の糖以外の糖類を含有する場合に比べて嗜好性を有 する。 さらに、 血糖値の上昇抑制効果も糖アルコールおよび二糖以上の糖以 外を含有する場合に比べて優れた効果を有する。 本発明の組成物は、 甘藷茎葉の加工物を含有しているため、 ポリフエノー ル含有量が高く、.栄養価に優れている。 特に第 1の組成物は、 甘藷茎葉中に 甘藷の若茎葉を含有するため、 ポリフヱノール含有量がさらに高く、 加工が 容易である。 第 2の組成物は、 さらに糖アルコールおよぴニ糖以上の糖から なる群より選択される少なくとも 1種を含有するため、 甘藷加工物を含有す る様々な製剤化が可能となるだけでなく、 嗜好性および安定性も向上する。 その結果、 甘藷茎葉加工物の利用が容易で安定な甘藷含有固体 (例えば、 粉 体、 鍵剤、 顆粒といった造粒物など) を容易に得ることができる。 本発明の 第 2の組成物は、 特に優れた抗糖尿病効果を有する。 (実施例)  The intake of the second composition of the present invention is not particularly limited. Preferably, the daily intake of an adult is 10 mg to 300 mg, preferably 10 mg to: L as the total amount of dicaffeoylquinic acid and the like contained in the processed product of sweet potato foliage. It is taken so as to be 0.000 mg. In addition, in the case of a dry powder of sweet potato stems and leaves, it may be blended in an amount of 0.1 g to 30 g, preferably 0.1 to 10 g. The second composition of the present invention exhibits excellent granulation properties and stability, and has a palatability as compared with the case where a saccharide other than saccharide alcohol or disaccharide is contained. Furthermore, the effect of suppressing an increase in blood glucose level is also superior to the case of containing a sugar alcohol and a sugar other than disaccharide or more. Since the composition of the present invention contains a processed product of sweet potato stems and leaves, it has a high polyphenol content and is excellent in nutritional value. In particular, since the first composition contains the sweet potato stem and leaves in the sweet potato stem and leaf, the polyphenol content is higher and the processing is easy. Since the second composition further contains at least one selected from the group consisting of sugar alcohols and disaccharides and higher sugars, various formulations containing a processed sweet potato product are only possible. In addition, palatability and stability are improved. As a result, a sweet potato-containing solid (eg, a granulated substance such as a powder, a key material, or a granule) can be easily obtained in which the processed sweet potato foliage can be easily used and is stable. The second composition of the present invention has a particularly excellent antidiabetic effect. (Example)
以下、 本発明をより詳細に説明するが、 以下の実施例に制限されず、 本発 明の範囲内で種々の変更が可能であることは言うまでもない。 Hereinafter, the present invention will be described in more detail, but is not limited to the following examples. It goes without saying that various changes are possible within the scope of the description.
(実施例 1 :甘藷の若茎葉の加工物を含有する組成物の製造) (Example 1: Production of a composition containing a processed product of young shoots of sweet potato)
すいおうの種芋を植え込み、 地上部の長さが 1 5 0 c m程度となるまで栽 培した。 そして、 甘藷の葉の先端部から 6 0 c mの部分を刈り取り、 水で 2 回洗浄して、 1 k gの甘藷の若茎葉を得た (甘藷茎葉 1とする) 。 Suio seeds were planted and cultivated until the length of the aerial part was about 150 cm. A portion of 60 cm from the tip of the sweet potato leaf was cut off and washed twice with water to obtain a 1 kg young shoot of sweet potato (referred to as sweet potato stem 1).
得られた甘藷の若茎葉を 5 mm程度にカットした後、 p H 8 . 0に調整し た 2 Lの熱水 ( 9 0 °C) に 1分間浸漬した。 次いで、 2 5 °Cの水で冷却した 後、 3 0秒間遠心分離して、 ある程度まで脱水した。 その後、 水分量が約 2 0質量%となるまで、 乾燥機中 7 0 °Cにて 2時間温風乾燥 (一次乾燥) し、 次いで、 最終水分量が 3質量%となるように、 8 0 °Cにて 4時間温風乾燥 The obtained young shoots of sweet potatoes were cut into about 5 mm and immersed in 2 L of hot water (90 ° C.) adjusted to pH 8.0 for 1 minute. Then, after cooling with water at 25 ° C, the mixture was centrifuged for 30 seconds and dehydrated to some extent. Then, dry in a dryer at 70 ° C for 2 hours with hot air (primary drying) until the water content becomes approximately 20% by mass. Drying with warm air at ° C for 4 hours
(二次乾燥) した。 この乾燥物を 1 5 0 °Cの飽和水蒸気圧を用いて、 3秒間 加圧蒸気殺菌した。 殺菌により甘藷の若茎葉に付着した水分を再度乾燥して 除去した後、 2 0 0メッシュ区分を 9 0質量%が通過するようにハンマーミ ルを用いて微粉粋して、 甘藷の若茎葉の乾燥粉末 ( 8 0 g ) を得た。 この乾 燥粉末 l gを用いて、 フォーリンデニス法 (財団法人日本食品分析センター 編集、 五訂日本食品標準成分表分析マニュアルの解説) により、 乾燥粉末 1 0 0 g当たりの総ポリフエノール量を、 カテキンを標準物質として測定した。 結果を表 1に示す。 (Secondary drying). The dried product was autoclaved using a saturated steam pressure of 150 ° C. for 3 seconds. After removing the moisture adhering to the young shoots of the sweet potato by sterilization again, finely pulverize it with a hammer mill so that 90% by mass of the 200 mesh class can pass through, and then dry the young shoots of the sweet potato. A powder (80 g) was obtained. Using the dried powder lg, the total amount of polyphenols per 100 g of dry powder was determined by the Foreign Dennis method (edited by the Japan Food Research Laboratories, and edited by the Fifth Edition Japanese Food Standard Composition Table Analysis Manual). Was measured as a standard substance. The results are shown in Table 1.
(実施例 2 ) (Example 2)
実施例 1で刈り取つた後の甘藷茎葉を、 さらに地上部の長さが 1 5 0 c m 程度となるまで栽培した。 そして再度、 甘藷の葉の先端から 6 0 c mの部分 を刈り取り、 甘藷の若茎葉を得た (甘藷茎葉 2とする) 。 この甘藷の若茎葉 を用いて実施例 1と同様に行い、 8 0 gの甘藷茎葉の粉末を得た。 上記操作 を再度繰り返し、 合計 1 6 0 gの甘藷茎葉乾燥粉末を得た後、 実施例 1と同 様にして総ポリフエノール量を測定した。 結果を表 1に示す。 (実施例 3 ) The sweet potato stems and leaves that were cut off in Example 1 were further cultivated until the length of the above-ground portion became about 150 cm. Then, a portion 60 cm from the tip of the leaf of the sweet potato was cut off again to obtain a young shoot of the sweet potato (referred to as a sweet potato stem 2). Using the young shoots of this sweet potato, the same procedure as in Example 1 was carried out to obtain 80 g of sweet potato shoot powder. The above operation was repeated again to obtain a total of 160 g of sweet potato stem and leaf dry powder. The total amount of polyphenol was measured as described above. Table 1 shows the results. (Example 3)
実施例 1の甘藷の若茎葉の代わりに、 実施例 1と同様に栽培した甘藷茎葉 の地上部をすベて刈り取り (すなわち、 若茎葉を含む甘藷茎葉を刈り取り) 、 甘藷茎葉 (全長) を得た (甘藷茎葉 3とする) 。 これを甘藷茎葉として用い たこと以外は、 実施例 1と同様に行い、 1 k gの甘藷茎葉から甘藷茎葉の乾 燥粉末 1 0 0 gを得た。 得られた乾燥粉末に含有される総ポリフエノール量 を測定した。 結果を表 1に示す。  Instead of the young shoots and leaves of the sweet potato of Example 1, all the above-ground portions of the sweet potatoes and leaves cultivated in the same manner as in Example 1 were cut off (that is, the sweet potatoes and leaves including the young shoots were cut off), and the sweet potatoes and leaves (full length) were obtained. (Sweet potatoes and leaves 3) Except that this was used as sweet potato stems and leaves, the same procedure as in Example 1 was carried out to obtain 100 g of dry powder of sweet potato stems and leaves from 1 kg of sweet potato stems and leaves. The total amount of polyphenol contained in the obtained dry powder was measured. The results are shown in Table 1.
(調製例 1 ) (Preparation Example 1)
実施例 1の甘藷の若茎葉の代わりに、 実施例 1と同様に栽培した甘藷茎葉 の地上部をすベて刈り取り、 さらにこの甘藷茎葉の先端から 6 0 c mの部分 を取り除き甘藷茎葉を得た (甘藷茎葉 4とする) 。 この甘藷茎葉を用いたこ と以外は、 実施例 1と同様に行い、 1 k gの甘藷茎葉から 1 1 0 gの甘藷茎 葉の乾燥粉末を得、 含有される総ポリフエノール量の測定を行った。 結果を 表 1に示す。  Instead of the young shoots of the sweet potatoes of Example 1, all the above-ground parts of the sweet potatoes and leaves cultivated in the same manner as in Example 1 were cut off, and a portion of 60 cm from the tip of the sweet potatoes and leaves was removed to obtain sweet potato shoots. (Sweet potatoes and leaves 4). Except for using the sweet potato stems and leaves, the same procedure as in Example 1 was carried out to obtain 110 g of a dry powder of sweet potato stems and leaves from 1 kg of sweet potato stems and leaves, and the total polyphenol content was measured. . Table 1 shows the results.
表 1 table 1
Figure imgf000021_0001
表 1の結果から、 甘藷の若茎葉 (甘藷の葉の先端部から 6 0 c mの部分) を含む乾燥粉末 (実施例 1〜 3 ) は、 調製例 1の甘藷の若茎葉を含まない乾 燥粉末に比べて、 高いポリフエノール含有量を有することが分かる。 特に、 実施例 2の一旦切り取られた部位から発生した若茎葉部分でも高いポリフエ ノール含有量を有していた。 これは、 甘藷茎葉の若茎葉 (特に甘藷の葉の先 端から 6 0 c mの部分) に、 有効成分、 特にポリフエノールが多く含まれる ことを示している。
Figure imgf000021_0001
From the results in Table 1, the young stem leaves of sweet potatoes (the part 60 cm from the tip of the sweet potato leaves) It can be seen that the dry powders containing Examples (Examples 1 to 3) have a higher polyphenol content than the dry powders containing no young shoot leaves of the sweet potato of Preparation Example 1. In particular, the young stem and leaf portions generated from the once cut portion of Example 2 also had a high polyphenol content. This indicates that the young stems and leaves of the sweet potatoes (especially the portion 60 cm from the tip of the sweet potato leaves) contain a large amount of the active ingredient, especially polyphenol.
さらに、 実施例 1〜3の乾燥粉末は、 調製例の乾燥粉末に比べて、 粉末の 舌触りがよく、 特に実施例 1および 2の乾燥粉末の舌触りがよかった。 これ は、 実施例 1〜3、 特に実施例 1〜2で用いた甘藷の若茎葉は、 茎部がやわ らかいためと考えられる。  Furthermore, the dry powders of Examples 1 to 3 had a better palatability than the dry powders of the Preparation Examples, and particularly the dry powders of Examples 1 and 2 had a better palatability. This is considered to be because the stem portions of the young shoots of the sweet potato used in Examples 1 to 3, particularly Examples 1 and 2 are lucid.
(実施例 4 :抽出物の製造及び分析) (Example 4: Production and analysis of extract)
実施例 1で得られた甘藷の若茎葉 (甘藷茎葉 1 ) 3 gに、 8 0容量%のェ タノール水溶液を 1 0 O m L加えて、 8 0 °Cにて 2 4時間加熱還流を行つた。 その後、 濾過をして、 抽出液を得、 さらに 5 0 °Cにて減圧濃縮し、 抽出粉末 を得た。 この抽出粉末 1 0 O m gを用いて、 実施例 1と同様にして、 抽出粉 末 1 0 0 g当たりの総ポリフエノール量を、 カテキンを標準物質として測定 した。 実施例 2おょぴ 3ならびに調製例 1の甘藷茎葉 2〜 4についても上記 と同様に操作して、 抽出粉末の質量および抽出粉末 1 0 0 g当たりの総ポリ フエノール量を測定した。 結果を表 2に示す。 To 3 g of the young shoots and leaves of the sweet potato obtained in Example 1 (sweet shoots and leaves 1) was added 100 mL of an aqueous solution of 80% by volume of ethanol, and the mixture was heated and refluxed at 80 ° C for 24 hours. I got it. Thereafter, filtration was performed to obtain an extract, which was further concentrated under reduced pressure at 50 ° C. to obtain an extract powder. Using 100 mg of this extracted powder, the total amount of polyphenol per 100 g of the extracted powder was measured using catechin as a standard substance in the same manner as in Example 1. The same procedure was applied to the sweet potato stems and leaves 2 to 4 of Example 2 and Preparation Example 1 to measure the mass of the extracted powder and the total amount of polyphenol per 100 g of the extracted powder. Table 2 shows the results.
表 2 Table 2
Figure imgf000023_0001
表 2の結果から、 甘藷茎葉 1〜3の乾燥粉末より抽出して得られた抽出粉 末の収量が多く、 また、 ポリフエノール含有量も高いことが分かる。 これは、 甘藷の若茎葉中にポリフエノールが高含有されているか、 あるいは甘藷茎葉 1〜 3の乾燥粉末中には甘藷茎葉の茎部よりも葉部の比率が高いため、 この 葉部に可溶性成分またはポリフエノールが高含有されていると考えられる。 その結果、 より抽出物が多く、 ポリフエノール含有量の高い抽出粉末が得ら れやすくなったものと考えられる。
Figure imgf000023_0001
From the results shown in Table 2, it can be seen that the yield of the extracted powder obtained by extracting from the dried powders of the sweet potato stems 1 to 3 is high, and the polyphenol content is high. This is due to the high content of polyphenols in the young shoots of sweet potatoes, or the fact that the dry powder of sweet potato shoots 1-3 has a higher leaf ratio than that of sweet potato shoots. It is considered that the component or polyphenol is high. As a result, it is considered that an extract powder with a larger amount of extract and a high polyphenol content was easily obtained.
(実施例 5 :甘藷茎葉エキス末の製造) (Example 5: Production of sweet potato stem and leaf extract powder)
実施例 1と同様にして得られた甘藷の若茎葉 (甘藷茎葉 1 ) 3 k gを、 マ スコロイダーでペースト状に破枠し、 濾過して甘藷茎葉の搾汁を得た。 この 搾汁を 4 0 °Cで減圧濃縮して乾固させ、 5 5 gの甘藷茎葉エキス末を得た。  3 kg of the young shoots of sweet potatoes (sweet potato shoots 1) obtained in the same manner as in Example 1 were cut into a paste form using a massoidloid and filtered to obtain juice of sweet potato shoots and leaves. The squeezed juice was concentrated under reduced pressure at 40 ° C. and dried to obtain 55 g of sweet potato stem and leaf extract powder.
(実施例 6 :甘藷茎葉抽出物の製造) (Example 6: Production of sweet potato stem and leaf extract)
実施例 1と同様にして得られた甘藷の若茎葉 (甘藷茎葉 1 ) 1 k gを、 そ のまま 8 0 °Cで熱風乾燥して、 乾燥物を得た。 この乾燥物をポールミルで粗 粉碎し、 水 1 0 L加えて、 8 0 °Cで 2 4時間かけて加熱還流を行った後に、 濾過をして抽出液を得た。 次いで、 5 0 °Cで減圧濃縮を行い、 2 0 gの抽出 粉末を得た。 この抽出粉末中のポリフエノ一ル量を実施例 1と同様に測定し たところ、 抽出粉末中に5 0質量%のポリフエノールが含有されていること 力 sわ力つた Q 1 kg of young shoots of sweet potatoes (sweet potato shoots 1) obtained in the same manner as in Example 1 was dried with hot air at 80 ° C. to obtain a dried product. The dried product was coarsely ground with a pole mill, 10 L of water was added, and the mixture was heated under reflux at 80 ° C for 24 hours, An extract was obtained by filtration. Then, the mixture was concentrated under reduced pressure at 50 ° C to obtain 20 g of an extracted powder. Was measured Porifueno Ichiru amount of extract powder in the same manner as in Example 1, it forces s I 5 0% by weight of polyphenol extraction in the powder is contained ChikaraTsuta Q
(実施例 7 :錠剤の製造) (Example 7: Production of tablets)
実施例 1で得られた甘藷茎葉 1の乾燥粉末を用いて、 表 3に示す組成にて 錠剤を製造した。 表 3  Using the dry powder of the sweet potato stems and leaves 1 obtained in Example 1, tablets having the composition shown in Table 3 were produced. Table 3
Figure imgf000024_0001
Figure imgf000024_0001
* 1 · · ·日 品  * 1
(実施例 8 :飲料の製造) (Example 8: Production of beverage)
実施例 5で得られた甘藷茎葉 1のエキス末、 果糖プドウ糖液糖、 レモン果 汁、 クェン酸、 香料、 グリシン、 プロリン、 およびァラニンを表 4に示す量 で配合し、 これに純水を加えて 1 Lの飲料を調製した。 表 4 The extract powder of sweet potato stem and leaf 1 obtained in Example 5, fructose pudose syrup, lemon juice, cuenic acid, fragrance, glycine, proline, and alanine were mixed in the amounts shown in Table 4, and pure water was added thereto. In addition, a 1 L beverage was prepared. Table 4
Figure imgf000025_0001
Figure imgf000025_0001
(実施询 9 :飲料の製造 2 )  (Implementation 9: Beverage production 2)
以下の手順にて飲科を調製した。 まず、 実施例 6で得られた甘藷茎葉 1の 抽出粉末 1 0 0 gを 1 Lの熱水で 3 0分間抽出した後、 濾過して、 甘藷茎葉 1の熱水抽出液を得た。 上記と同様に、 緑茶葉 1 0 gおよび紅茶葉 1 0 gを • - 15 それぞれ 1 Lの熱水で抽出し、 濾過して緑茶葉の熱水抽出液および紅茶葉の 熱水抽出液をそれぞれ得た。 これらの抽出液およびァスコルビン酸を用いて、 表 5に示す組成にて茶飲科を調製した。 表 5  A drink course was prepared according to the following procedure. First, 100 g of the extracted powder of the sweet potato stem and leaf 1 obtained in Example 6 was extracted with 1 L of hot water for 30 minutes, and then filtered to obtain a hot water extract of the sweet potato stem and leaf 1. As above, extract 10 g of green tea leaves and 10 g of black tea leaves with 1 L of hot water •-15 each, filter and extract hot water extracts of green tea leaves and black tea leaves. Obtained. Using these extracts and ascorbic acid, a tea drinking department was prepared with the composition shown in Table 5. Table 5
20
Figure imgf000025_0002
20
Figure imgf000025_0002
25 (実施例 1 0 :青汁の製造) 25 (Example 10: Production of green juice)
実施例 3で得られた甘藷茎葉 3の乾燥粉末を用いて、 表 6に示す組成にて 甘藷茎葉の乾燥粉末を含有する青汁 (顆粒品) を製造した。 表 6 Using the dried powder of sweet potato stem and leaf 3 obtained in Example 3, with the composition shown in Table 6 A green juice (granule) containing dry powder of sweet potato stems and leaves was produced. Table 6
Figure imgf000026_0001
(実施例 1 1 :化粧水の製造)
Figure imgf000026_0001
(Example 11: Production of lotion)
実施例 6で得られた甘藷茎葉 1の抽出粉末を用いて、 表 7に示す組成にて 化粧水を調製した。 表 7
Figure imgf000026_0002
Using the extracted powder of the sweet potato stem and leaf 1 obtained in Example 6, a lotion having the composition shown in Table 7 was prepared. Table 7
Figure imgf000026_0002
(実施例 1 2 :甘藷茎葉微粉末の調製) (Example 12: Preparation of Sweet Potato Stem Fine Powder)
甘藷茎葉微粉末を以下のようにして調製した。 まず、 若茎葉を含む地表力: ら 200 cmに成長した甘藷茎葉 4 O k gを収穫し (甘藷茎葉 5とする) 、 pH8. 0に調整した熱水 (97°C) に 1分間浸漬させ、 ブランチング処理 を行った。 ブランチング処理後、 直ちに 20°Cの水へ浸漬して冷却した後、 熱風乾燥を行った。 次いで、 ダイサ一で長径が lmm程度になるように粉碎 し、 加圧蒸気殺菌機で殺菌後、 ハンマーミルを用いて粉末の粒径の 90質 量%以上が 600メッシュ以下 ( 20 μ m以下) となるように微粉碎した。 得られた甘藷茎葉 5の微粉末は 3. 2 k gであった。 A sweet potato stem fine powder was prepared as follows. First, the surface power including the young shoots: 4 kg of sweet potato stems and leaves grown to a height of 200 cm were harvested (referred to as sweet potato stems and leaves 5), immersed in hot water (97 ° C) adjusted to pH 8.0 for 1 minute, and subjected to blanching treatment. Immediately after the blanching treatment, it was immersed in water at 20 ° C to cool, and then dried with hot air. Next, the powder is ground with a dicer so that the major axis is about lmm, sterilized by a pressurized steam sterilizer, and 90 mass% or more of the particle diameter of the powder is 600 mesh or less (20 μm or less) using a hammer mill. And pulverized so that The resulting fine powder of the sweet potato stems and leaves 5 was 3.2 kg.
上記微粉末中のポリフエノール含有量をフォーリンチオカルト法にて測定 したところ、 100 §当たり 0. 4 gのポリフエノール (0. 4質量0 /0) が 含まれていることが分かった。 Measurement of the polyphenol content of the fine powder with Folin-Ciocalteu method, it was found that contain 100 § per 0.4 g of the polyphenol (0.4 mass 0/0).
(実施例 13) (Example 13)
実施例 12で得られた甘藷茎葉 5の微粉末、 デンプン、 還元麦芽糖、 トレ ハロース、 難消化性デキストリン、 および果糖を用いて、 表 8に記載の組成 にて混合粉末を調製した。 そして、 混合粉末 700 gを用いて、 流動造粒を 行った。 流動造粒は、 給気温度 95° (:、 排気温度 30 °Cにて、 1分間あたり 10 m Lの水を 15分間噴霧した後に 20分間乾燥を行い、 粉末 1〜 4を得 た。 得られた各粉末 (粉末 1〜4) について、 造粒性、 水への分散性、 およ ぴ嗜好性を以下のようにして評価した。  A mixed powder having the composition shown in Table 8 was prepared using the fine powder of sweet potato stem and leaf 5 obtained in Example 12, starch, reduced maltose, trehalose, indigestible dextrin, and fructose. Fluid granulation was performed using 700 g of the mixed powder. In the fluidized granulation, 10 mL of water per minute was sprayed for 15 minutes at an air supply temperature of 95 ° (:, exhaust temperature of 30 ° C.), followed by drying for 20 minutes to obtain powders 1 to 4. Each of the obtained powders (powder 1 to 4) was evaluated for granulation properties, dispersibility in water, and palatability as follows.
(1) 造粒性  (1) Granulation properties
流動造粒後の粉末を、 140メッシュ ( 106 μ m) の篩を備えた篩別機 を用いて篩分けを行い、 顆粒が形成されているか否かを以下の基準で判定し た。  The powder after the fluid granulation was sieved using a sieving machine equipped with a 140-mesh (106 μm) sieve, and whether or not granules were formed was determined according to the following criteria.
<造粒性の判定基準 >  <Gritability criteria>
粉末の通過量が 30質量%未満である (造粒性が非常に良い) :◎ 粉末の通過量が 30質量%〜50質量%である (造粒性が良い) :〇 粉末の通過量が 5 0質量%を超える (造粒性が悪い) : XThe passing amount of the powder is less than 30% by mass (very good granulation): ◎ The passing amount of the powder is 30% to 50% by mass (good granulation): 〇 Passing amount of powder exceeds 50% by mass (poor granulation): X
( 2 ) 水への分散十生 (2) Dispersion in water
流動造粒後の粉末 3 gを水 1 0 0 m Lに添加し、 1 0秒間攪拌した後に 1 分間静置し、 目視により以下の基準で評価した。  3 g of the powder after the fluid granulation was added to 100 mL of water, stirred for 10 seconds, allowed to stand for 1 minute, and visually evaluated according to the following criteria.
く水への分散性の判定基準〉 Criteria for dispersibility in water>
均一に分散している :◎ 若干の溶け残り (凝集物) が見られるが、 分散している :〇 分散しない溶け残り (凝集物) および沈殿物が見られる : X  Uniformly dispersed: ◎ Slightly undissolved (aggregate) is observed, but dispersed: 〇 Undissolved undissolved (aggregate) and precipitate are observed: X
( 3 ) 嗜好性  (3) Preference
流動造粒後の粉末 3 gを水 1 0 O m Lに添加し、 均一になるまで攪拌して 飲料を調製した。 1 0名の女性パネラーに試飲してもらい、 以下の判定基準 で採点してもらった。 得られた点数を合計した嗜好性を評価した。  3 g of the powder after the fluid granulation was added to 10 mL of water and stirred until the mixture became uniform to prepare a beverage. Ten female panelists sampled and scored according to the following criteria. The preference obtained by summing the obtained scores was evaluated.
<嗜好性の採点基準 > <Scoring criteria for palatability>
口当たりおよび後味が共によい : 1点  Good mouthfeel and aftertaste: 1 point
口当たりはよいが後味が悪い : 0点  Pleasant but bad aftertaste: 0
口当たりおよび後味共に悪!ヽ :一 1点  Bad taste and aftertaste!ヽ: One point
結果を表 8に示す。 Table 8 shows the results.
表 8 Table 8
Figure imgf000029_0001
表 8の結果から、 本努明の甘藷茎葉の微粉末と糖アルコールまたはニ糖以 上の糖とを用いた粉末 1〜 3は、 造粒性に特に優れ、 水への分散性にも優れ ていた。 また、 嗜好性においても、 甘藷茎葉の微粉末と糖アルコールまたは 二糖以上の糖とを用いた粉末 1〜3から得られる飲料は、 口当たりおよぴ後 味が特によかった。
Figure imgf000029_0001
From the results in Table 8, it can be seen that the powders 1 to 3 using the fine powder of sweet potato stems and leaves and sugar alcohol or sugars other than disaccharide of the present invention have particularly excellent granulation properties and excellent dispersibility in water. I was In terms of palatability, beverages obtained from fine powder of sweet potato stem and leaves and powders 1 to 3 using sugar alcohol or disaccharide or more sugars had particularly good mouthfeel and aftertaste.
(実施例 1 4 :甘藷茎葉の抽出物の調製) ' (Example 14: Preparation of Sweet Potato Stem Extract) ''
実施例 1 2で得られた甘藷茎葉 5の微粉末 1 0 0 gを準備し、 この甘藷茎 葉微粉末を、 エタノールと純水とを 8 : 2 (重量比) で混合した含水エタノ ール 5 Lに分散させて、 1時間加熱還流を行い、 抽出した。 次に、 濾過によ り濾液を得て、 その濾液を凍結乾燥させることにより、 甘藷茎葉 5の抽出物 粉末 5 gを得た。 (実施例 1 5 :錠剤の製造)  100 g of the fine powder of sweet potato stem and leaf 5 obtained in Example 12 was prepared, and this sweet potato stem and fine powder was mixed with ethanol and pure water at a ratio of 8: 2 (weight ratio) in hydrated ethanol. The mixture was dispersed in 5 L, heated under reflux for 1 hour, and extracted. Next, a filtrate was obtained by filtration, and the filtrate was freeze-dried to obtain 5 g of an extract powder of sweet potato stem and leaf 5. (Example 15: Production of tablets)
実施例 1 4で得られた甘藷茎葉 5の抽出物粉末、 結晶セルロース、 ショ糖 エステル、 二酸化けい素、 麦芽糖、 エリスリ トール、 パラチノース、' フラク トオリゴ糖、 および果糖を、 表 9に記載の組成にて錠剤 (1錠あたり 2 0 0 m g ) を調製した。 Extract powder of sweet potato stem and leaf 5 obtained in Example 14, crystalline cellulose, sucrose Ester, silicon dioxide, maltose, erythritol, palatinose, 'fructooligosaccharide, and fructose were prepared into tablets (200 mg per tablet) according to the composition shown in Table 9.
次いで、 これらをそれぞれ、 3錠ずつアルミバウチに分包したものをそれ ぞれ 6包準備し、 そのうちの 3包 (計 9錠) は、 温度 5 0 °Cおよび湿度 7 5 %の条件下で 4週間保管した (条件 1とする) 。 また残りの 3包 (計 9 錠) は、 4 °Cの冷暗室に 4週間保管し、 変色等が起こらないようにした (条 件 2とする) 。 4週間後にそれぞれのアルミバウチに分包された錠剤の状態 を観察し、 以下の基準で採点し、 採点の合計点により安定性を評価した。 結 果を表 9に合わせて示す。 なお、 表中の採点は、 各錠剤 1〜5における採点 の合計点である。  Next, three tablets each of these were packaged in aluminum bouches, and six tablets each were prepared. Of these, three tablets (a total of nine tablets) were prepared under the conditions of a temperature of 50 ° C and a humidity of 75%. Stored for a week (Condition 1). The remaining three packets (a total of 9 tablets) were stored in a cool and dark room at 4 ° C for 4 weeks to prevent discoloration (condition 2). Four weeks later, the state of the tablets packaged in the respective aluminum bouches was observed, and the tablets were scored based on the following criteria, and the stability was evaluated based on the total score. The results are shown in Table 9. The scores in the table are the total scores of the tablets 1 to 5.
く判定基準〉 .  Criteria).
条件 2と条件 1との間に変化は見られない : 1点 条件 2に対し、 条件 1は吸水または変色が見られる : 0点 条件 2に対し、 条件 1は吸水おょぴ変色が見ら る · :一 1点 表 9 No change between condition 2 and condition 1: 1 point Condition 1 shows water absorption or discoloration for condition 2: 0 point Condition 2 shows water absorption or discoloration for condition 2 · · 1 point 1 Table 9
Figure imgf000030_0001
Figure imgf000030_0001
表 9の結果から、 本発明の甘藷茎葉の抽出物粉末と糖アルコールまたは二 糖以上の糖とを含有する錠剤:!〜 4は、 特に優れた安定性を示した。 From the results in Table 9, it can be seen that the extract powder of the sweet potato stem and leaves according to the present invention and sugar alcohol or Tablets containing more than sugar :! ~ 4 showed particularly good stability.
(実施例 16 : I I型糖尿病予防並びに治療効果 (血糖値上昇抑制または 低下効果) ) (Example 16: Preventive and therapeutic effects on type I diabetes (suppressing or lowering blood sugar levels))
マウスの標準飼料 (MF、 オリエンタル酵母工業株式会社) に、 実施例 1 Example 1 with standard mouse feed (MF, Oriental Yeast Co., Ltd.)
2で得られた甘藷茎葉 5の微粉末、 デキストリン (松谷化学工業株式会社 製) 、 還元麦芽糖、 および果糖を、 表 10に記載の含有量となるように混合 して試験飼料 1〜 3を調製した。 以下のようにして I I型糖尿病に対する効 果を検証した。 The test powders 1-3 were prepared by mixing the fine powder of the sweet potato stems and leaves 5 obtained in Step 2, dextrin (manufactured by Matsutani Chemical Industry Co., Ltd.), reduced maltose, and fructose so as to have the contents shown in Table 10. did. The effect on type I diabetes was examined as follows.
まず、 5週齢の雄性の 2型糖尿病モデルマウス (KK— Ayマウス) (日 本チャールズリバ一社) 28匹を標準飼料を与えて 1週間馴化した後に、 眼 底より採血を行い、 血糖値をダルテストセンサー (三和科学社製) を用いて 測定した。 次いで、 血糖値の平均値が均一になるように (255〜 257m g/dL) 上記マウスを 1群 7匹の 4群に分けた。 3群のラットに試験飼料 1〜3をそれぞれ自由摂取させた。 残りの 1群のラットには、 標準飼料に果 糖を混合した対照飼料を自由摂取させた。 なお、 試験中水については自由飲 水とした。 摂取開始から 28日目に眼窩静脈より血液を採取し、 血液中の血 糖値を測定した。 結果を表 10に合わせて示す。 なお、 表 10中の血糖値の 値は平均値土標準偏差 (mg_ dL) を示す。 First, 28-week-old male type 2 diabetes model mice (KK-Ay mice) (Charles River Japan, Inc.) were acclimated to a standard diet for 28 days, blood was collected from the fundus, and blood glucose was measured. Was measured using a dull test sensor (manufactured by Sanwa Kagaku). Next, the mice were divided into four groups, seven per group, so that the average blood glucose level was uniform (255 to 257 mg / dL). Three groups of rats were allowed to freely take test feeds 1-3. The remaining one group of rats had free access to a control diet in which fructose was mixed with the standard diet. Water during the test was free drinking. Blood was collected from the orbital vein on the 28th day from the start of ingestion, and the blood glucose level in the blood was measured. The results are shown in Table 10. The blood glucose values in Table 10 represent the mean soil standard deviation (mg_dL).
表 1 0 Table 10
Figure imgf000032_0001
Figure imgf000032_0001
表 1 0の結果において、 試験飼料 3を摂取した場合と対照飼料を摂取した 場合との比較から、 若茎葉を含む甘藷¾葉加工物は、 果糖による血糖値上昇 を抑制する効果を有することがわかる。 また、 甘藷茎葉の加工物 (微粉末) と糖アル —ルまたは二糖以上の糖とを含有する試験飼料 1および 2は、 さ らに優れた血糖値上昇抑制効果を有することが分かる。 すなわち、 本発明の 甘藷茎葉の加工物と糖アルコールまたは二糖以上の糖とを含有する組成物は、 特に優れた抗糖尿病効果 (糖尿病の予防または治療効果) を有することが分 かる。  In the results of Table 10, from the comparison between the case where the test feed 3 was ingested and the case where the control feed was ingested, the processed sweet potato leaves containing the young shoots and leaves were found to have the effect of suppressing the increase in blood sugar level due to fructose. Understand. In addition, it can be seen that the test feeds 1 and 2 containing the processed product (fine powder) of sweet potato stems and leaves and a sugar alcohol or a disaccharide or more sugars have an even more excellent blood sugar level suppressing effect. That is, it is found that the composition containing the processed product of the sweet potato stem and leaves of the present invention and a sugar alcohol or a disaccharide or higher saccharide has a particularly excellent antidiabetic effect (the effect of preventing or treating diabetes).
(実施例 1 7 ) . (Example 17).
若茎棄を含む地上部が 2 0 O c mの甘藷茎葉をすベて収穫した。 この甘藷 茎葉 (ジカフヱオイルキナ酸等を含有) 1 k gを、 p H 8 . 0に調整した熱 水 (9 7 °C) に 1分間浸漬し、 ブランチング処理を行った。 ブランチング処 理後、 直ちに 2 0 °Cの水へ浸漬して冷却した後に、 熱風乾燥を行った。 得ら れた乾燥物をダイサ一で長径が 1 mm程度になるように粉枠し、 加圧蒸気殺 菌機で殺菌後、 ハンマーミルを用いて 8 0 gの甘藷茎葉の微粉末を得た。 こ の微粉末のポリフエノール含有量を、 カテキンを標準物質として、 フォーリ ンチオカルト法により測定したところ、 微粉末 1 g当たり 1 O m gのポリフ ェノール (1質量%) が含まれていた。 (実施例 18 :食品の調製) All the sweet potato stems and leaves with an aerial part of 20 O cm including young shoots were harvested. 1 kg of the foliage of the sweet potato (containing dicafoil quinic acid, etc.) was immersed in hot water (97 ° C.) adjusted to pH 8.0 for 1 minute to perform a blanching treatment. Immediately after the blanching treatment, the substrate was immersed in water at 20 ° C, cooled, and then dried with hot air. The obtained dried product was powder-framed with a dicer so that the major axis was about 1 mm, sterilized with a pressurized steam sterilizer, and then a hammer mill was used to obtain 80 g of fine powder of sweet potato stem and leaves. . When the polyphenol content of this fine powder was measured by the Folin Ciocult method using catechin as a standard substance, it was found that 1 g of fine powder contained 1 mg of polyphenol (1% by mass). (Example 18: Preparation of food)
実施例 17で得られた甘藷茎葉の微粉末 (ジカフェオイルキナ酸等を有す る原料) 、 グアバ葉抽出物 (商品名 .:グアバフエノン、 備前化成ネ環) 、 難 消化性デキストリン (商品名:パインファイバー、 松谷化学工業社製) 、 ビ タミン B 1、 および還元麦芽糖を用いて、 表 1.1の組成にて食品 (粉末) を 調製した (各々食品 1〜4) 。 なお、 グアバ抽出物、 難消化性デキストリン、 およぴビタミン Bェは、 糖の代謝促進成分または糖の消化吸収抑制成分を有 する原料である。  Fine powder of sweet potato stems and leaves obtained in Example 17 (raw material having dicaffeoylquinic acid, etc.), guava leaf extract (trade name: guaba phenone, Bizen Kasei Neyring), indigestible dextrin (trade name) : Pine fiber, manufactured by Matsutani Chemical Industry Co., Ltd.), vitamin B1, and reduced maltose were used to prepare foods (powder) with compositions shown in Table 1.1 (foods 1 to 4, respectively). Guava extract, indigestible dextrin, and vitamin B are raw materials having a sugar metabolism promoting component or a sugar digestion / absorption inhibiting component.
表 11 Table 11
Figure imgf000033_0001
Figure imgf000033_0001
上記の食品のうち、 食品 1〜3を用いて抗糖尿病効果を以下のようにして 評価した。 Among the above foods, foods 1 to 3 were used to evaluate the antidiabetic effect as follows.
まず、 6週齢の雄性の SDラット (九動株式会社) 25匹を、 標準飼料 (MF、 オリエンタル酵母工業株式会社) を与えて 1週間馴化した。 順化後、 ストレブトゾトシンを 3 Omg/k g体重となるように尾静脈から投与し、 4日後に眼窩静脈より血液を採取し、 摂食時における血糖値を測定した。 そ して、 血糖値が 20 OmgZdL以上となった 20匹を選定し、 I型糖尿病 モデルとした。 さらにこの 20匹を、 血糖値の平均値が同等 (431〜43 3mgZdL) になるように 1群 5匹の 4群に分けた。 このうちの 2群には、 食品 1および 2をそれぞれ質量が 100 OmgZk g体重になるように 7日 間にわたり、 1日 1回の強制経口投与を行った (試験群) 。 なお、 各経口投 与には溶媒として 0. 5 %T w e e n生理食塩水を使用した。 別の 1群には、 食品 3を上記と同様にして投与した (比較試験群) 。 残りの 1群には溶媒の みを投与した (対照群) 。 試験中は、 標準飼料を自由摂食とし、 水について も自由飲水とした。 摂取から 7日後の再度摂食時の血液を採取し、 血液中の 血糖値 (mg/dL) を測定した。 その結果 (平均値および標準偏差) を、 表 12に示す。 表 12
Figure imgf000034_0001
First, 25 6-week-old male SD rats (Kudo Co., Ltd.) were fed with a standard diet (MF, Oriental Yeast Co., Ltd.) for one week. After acclimation, streptozotocin was administered via the tail vein to a concentration of 3 Omg / kg body weight. Four days later, blood was collected from the orbital vein and the blood glucose level during feeding was measured. Then, 20 animals whose blood glucose level was 20 OmgZdL or more were selected, and type I diabetes was selected. Model. These 20 animals were further divided into 4 groups, 5 animals per group, so that the mean blood glucose level was equivalent (431 to 433 mgZdL). Two of the groups were given oral gavage of foods 1 and 2 once a day for 7 days so that each of them weighed 100 OmgZkg body weight (test group). In each oral administration, 0.5% Tween physiological saline was used as a solvent. In another group, food 3 was administered in the same manner as described above (comparative test group). The other group received only vehicle (control group). During the test, the standard feed was freely available and water was also freely available. Seven days after ingestion, blood was again collected at the time of feeding, and blood glucose (mg / dL) in the blood was measured. Table 12 shows the results (mean and standard deviation). Table 12
Figure imgf000034_0001
表 12の結果から、 試験群で用いられた甘藷茎葉の微粉末 (ジカフェオイ ルキナ酸等を有する原料) と糖の代謝促進成分または糖の消化吸収抑制成分 を有する原料と含有する食品 (食品 1および食品 2) は、 比較試験群で用い られた糖の代謝促進成分または糖の消化吸収抑制成分を有する原料同士を組 合せた食品 (食品 3) および対照群 (標準飼料のみ) よりも、 優れた血糖値 上昇抑制効果、 すなわち抗糖尿病効果を有することが分かった。 特に、 ジカ フエオイルキナ酸等を有する原料と難消化性デキストリンとを含有する食品 (食品 2 ) では、 便通改善作用を有する難消化性デキストリンを含有してい るため、 便通改善も期待できる。 (実施例 1 9 :甘藷茎葉乾燥粉末の分析) From the results in Table 12, it can be seen that the fine powder of the sweet potato foliage (raw material containing dicaffeoylquinic acid, etc.) used in the test group and the raw material containing the sugar metabolism-promoting component or the sugar digestion-absorption-inhibiting component (food 1 and food 1) Food 2) was superior to food (food 3) and the control group (standard feed only) in which raw materials having a sugar metabolism-promoting component or a sugar digestion-absorption-inhibiting component were used in the comparative test group. It was found to have an effect of suppressing an increase in blood glucose level, that is, an antidiabetic effect. In particular, Zika Foods containing a raw material containing hueoylquinic acid and indigestible dextrin (Food 2) contain indigestible dextrin having an effect of improving bowel movement, so that improvement in bowel movement can also be expected. (Example 19: Analysis of sweet potato stem dry powder)
実施例 1で得られた甘藷茎葉 1の乾燥粉末中のカフェ一酸換算の総ポリフ ェノ一ル量を以下のようにして測定した。 まず、 1 0 0 m gの乾燥粉末に水 を l O O m L加え、 2 0分間超音波抽出を行った。 得られた抽出液について、 フォーリン ·デニス法により 7 3 0 n mの吸光度を測定した。 なお、 標準物 質として、 市販のカフヱ一酸 (シグマアルドリッチジャパン株式会社) を用 いた。 上記と同様に 7 3 0 n mの吸光度を測定して検量線を作成し、 これを 用いてカフェ一酸換算にて総ポリフエノール量を算出した。  The total polyphenol amount in terms of caffeic acid in the dried powder of the sweet potato stem and leaf 1 obtained in Example 1 was measured as follows. First, lOOOmL of water was added to 100mg of the dry powder, and ultrasonic extraction was performed for 20 minutes. The resulting extract was measured for absorbance at 70 nm by the Folin-Dennis method. In addition, a commercially available cuffic acid (Sigma-Aldrich Japan KK) was used as a standard substance. A calibration curve was prepared by measuring the absorbance at 730 nm in the same manner as described above, and the total amount of polyphenol was calculated using this curve in terms of caffeic acid.
その結果、 甘藷茎葉 1の乾燥粉末中の総ポリフエノール量は、 カフェ一酸 換算で 0 . 9 9 g Z l 0 0 g乾燥粉末であった。 産業上の利用可能性  As a result, the total amount of polyphenol in the dried powder of the sweet potato stems and leaves 1 was 0.99 g Z100, in terms of caffeic acid. Industrial applicability
本発明の組成物に含有される甘藷茎葉の加工物は、 甘藷茎葉の若葉を含む ことで、 甘藷茎葉に含まれる有効成分、 特にポリフエノールを多く含有する ため、 食品、 医薬品、 医薬部外品、 化粧品などの原料として利用することが できる。 このような甘藷茎葉の加工物を含有する組成物は、 甘藷茎葉の栄養 成分を含有し、 嗜好性にも優れるだけでなく、 多くのポリフエノール成分が 含有されるため、 様々な生理作用が期待でき、 有用性が高い。 さらに、 甘藷 茎葉の加工物と、 糖アルコールおよび二糖以上の糖からなる群より選択され る少なくとも 1種の糖類とを組み合わせることによって、 造粒性、 安定性、 嗜好性、 および抗糖尿病効果に優れた組成物を得ることができる。  The processed product of sweet potato stems and leaves contained in the composition of the present invention contains the active ingredient contained in the sweet potato stems and leaves, particularly polyphenol, by containing young leaves of sweet potato stems and leaves. It can be used as a raw material for cosmetics. A composition containing such a processed product of sweet potato stems and leaves contains nutrients of sweet potato stems and leaves and is not only excellent in palatability, but also contains many polyphenol components, and is expected to have various physiological effects. Yes, highly useful. Furthermore, by combining the processed product of sweet potato foliage with at least one saccharide selected from the group consisting of sugar alcohols and disaccharides or more, granulation, stability, palatability, and anti-diabetic effects can be improved. An excellent composition can be obtained.

Claims

請求の範囲 The scope of the claims
1. 甘藷茎葉の加工物を含有する組成物であって、 該甘藷茎葉中に若茎葉が 含まれる、 組成物。 1. A composition containing a processed product of sweet potato stems and leaves, wherein the sweet potato stems and leaves contain young stems and leaves.
2. 前記甘藷茎葉が、 地上部の長さが 300 cm以下の甘藷茎葉の地上部の 少なくとも一部である、 請求項 1に記載の組成物。 2. The composition according to claim 1, wherein the sweet potato leaves are at least a part of the above-ground portions of the sweet potato leaves having a length of 300 cm or less.
3. 前記甘藷の若茎葉が、 甘藷の葉の先端から 60 cm以下の部位である、 請求項 1または 2に記載の,袓成物。 3. The composition according to claim 1 or 2, wherein the young shoots of the sweet potato are sites 60 cm or less from the tip of the sweet potato leaf.
4. 甘藷茎葉の加工物と、 糖アルコールおよび二糖以上の糖からなる群より 選択される少なくとも 1種の糖類とを含有する、 組成物。 4. A composition comprising a processed product of sweet potato foliage and at least one saccharide selected from the group consisting of sugar alcohols and two or more sugars.
5. 前記甘藷茎葉中に若茎葉が含まれる、 請求項 4に記載の組成物。 5. The composition according to claim 4, wherein the sweet potato leaves include young shoots.
6. 前記糖類が、 難消化性デキストリンである、 請求項 4または 5に記載の 組成物。 6. The composition according to claim 4, wherein the saccharide is an indigestible dextrin.
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JP2007119346A (en) * 2004-05-21 2007-05-17 National Agriculture & Food Research Organization Diabetes or diabetes complication preventive
JP2008220231A (en) * 2007-03-12 2008-09-25 Kagawa Univ Blood sugar level increase inhibitor and food product for suppressing blood sugar level increase containing maltotriitol as an active ingredient
JP2012039889A (en) * 2010-08-12 2012-03-01 Oku Tain Fermented food using tea stem and leaf and/or sweet potato stem and leaf, and method for producing the same
JP2012139185A (en) * 2010-12-30 2012-07-26 Taiyu Shinko Kosha:Kk Method for producing hop spice
CN103393882A (en) * 2013-07-30 2013-11-20 中国农业科学院农产品加工研究所 Sweet potato stem leaf polyphenol and preparation method thereof
JP5828053B1 (en) * 2015-03-27 2015-12-02 株式会社東洋新薬 Method for producing dried fruits and vegetables
JP2016013120A (en) * 2015-02-05 2016-01-28 株式会社東洋新薬 Fruit and vegetables powder production method
CN110140847A (en) * 2019-05-30 2019-08-20 中国农业科学院农产品加工研究所 A kind of sweet potato stem and leaf instant powder and preparation method thereof

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007119346A (en) * 2004-05-21 2007-05-17 National Agriculture & Food Research Organization Diabetes or diabetes complication preventive
JP2008220231A (en) * 2007-03-12 2008-09-25 Kagawa Univ Blood sugar level increase inhibitor and food product for suppressing blood sugar level increase containing maltotriitol as an active ingredient
JP2012039889A (en) * 2010-08-12 2012-03-01 Oku Tain Fermented food using tea stem and leaf and/or sweet potato stem and leaf, and method for producing the same
JP2012139185A (en) * 2010-12-30 2012-07-26 Taiyu Shinko Kosha:Kk Method for producing hop spice
CN103393882A (en) * 2013-07-30 2013-11-20 中国农业科学院农产品加工研究所 Sweet potato stem leaf polyphenol and preparation method thereof
CN103393882B (en) * 2013-07-30 2016-04-13 中国农业科学院农产品加工研究所 A kind of sweet potato stem leaf polyphenol and preparation method thereof
JP2016013120A (en) * 2015-02-05 2016-01-28 株式会社東洋新薬 Fruit and vegetables powder production method
JP5828053B1 (en) * 2015-03-27 2015-12-02 株式会社東洋新薬 Method for producing dried fruits and vegetables
CN110140847A (en) * 2019-05-30 2019-08-20 中国农业科学院农产品加工研究所 A kind of sweet potato stem and leaf instant powder and preparation method thereof

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