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WO2005026368A3 - Dihydroflavonol-4-reductase - Google Patents

Dihydroflavonol-4-reductase Download PDF

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Publication number
WO2005026368A3
WO2005026368A3 PCT/US2004/029541 US2004029541W WO2005026368A3 WO 2005026368 A3 WO2005026368 A3 WO 2005026368A3 US 2004029541 W US2004029541 W US 2004029541W WO 2005026368 A3 WO2005026368 A3 WO 2005026368A3
Authority
WO
WIPO (PCT)
Prior art keywords
dihydroflavonol
reductase
recombinant dna
dna construct
relates
Prior art date
Application number
PCT/US2004/029541
Other languages
English (en)
Other versions
WO2005026368A2 (fr
Inventor
Gary M Fader
Omolayo O Famodu
Lisa L Huang
Alexandre Da Silva Conceicao
Brian Mcgonigle
Yong Tao
Original Assignee
Du Pont
Gary M Fader
Omolayo O Famodu
Lisa L Huang
Alexandre Da Silva Conceicao
Brian Mcgonigle
Yong Tao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Gary M Fader, Omolayo O Famodu, Lisa L Huang, Alexandre Da Silva Conceicao, Brian Mcgonigle, Yong Tao filed Critical Du Pont
Priority to CA002538314A priority Critical patent/CA2538314A1/fr
Priority to EP04783682A priority patent/EP1668138A2/fr
Publication of WO2005026368A2 publication Critical patent/WO2005026368A2/fr
Publication of WO2005026368A3 publication Critical patent/WO2005026368A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un fragment d'acides nucléiques isolés codant une dihydroflavonol-4-réductase. L'invention concerne également la production d'une construction d'ADN de recombinaison codant l'ensemble ou une partie de dihydroflavonol-4-réductase en orientation sens ou antisens, l'expression de la construction d'ADN de recombinaison entraînant la production de taux modifiés de dihydroflavonol-4-réductase dans une cellule hôte transformée.
PCT/US2004/029541 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase WO2005026368A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002538314A CA2538314A1 (fr) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase
EP04783682A EP1668138A2 (fr) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50178803P 2003-09-10 2003-09-10
US60/501,788 2003-09-10

Publications (2)

Publication Number Publication Date
WO2005026368A2 WO2005026368A2 (fr) 2005-03-24
WO2005026368A3 true WO2005026368A3 (fr) 2005-06-02

Family

ID=34312307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/029541 WO2005026368A2 (fr) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase

Country Status (3)

Country Link
EP (1) EP1668138A2 (fr)
CA (1) CA2538314A1 (fr)
WO (1) WO2005026368A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113755460B (zh) * 2021-09-10 2023-04-14 浙江华睿生物技术有限公司 一种用于制备二氢槲皮素的黄酮还原酶

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036716A1 (fr) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Plantes transgeniques presentant des couleurs florales modifiees et procedes permettant de les obtenir
EP1182257A1 (fr) * 2000-08-14 2002-02-27 Korea Kumho Petrochemical Co. Ltd. Acides nucléiques codant pour une dihydroflavonol 4-réductase dont la spécificité est modifiée et leur utilisations
WO2002101023A2 (fr) * 2001-06-13 2002-12-19 E.I. Du Pont De Nemours And Company Methode permettant de modifier le profil des isoflavonoides contenus dans les parties vegetales d'une plante produisant des isoflavonoides
WO2003031622A1 (fr) * 2001-10-05 2003-04-17 Agriculture Victoria Services Pty Ltd Manipulation de la biosynthese des flavonoides chez les plantes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036716A1 (fr) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Plantes transgeniques presentant des couleurs florales modifiees et procedes permettant de les obtenir
EP1182257A1 (fr) * 2000-08-14 2002-02-27 Korea Kumho Petrochemical Co. Ltd. Acides nucléiques codant pour une dihydroflavonol 4-réductase dont la spécificité est modifiée et leur utilisations
WO2002101023A2 (fr) * 2001-06-13 2002-12-19 E.I. Du Pont De Nemours And Company Methode permettant de modifier le profil des isoflavonoides contenus dans les parties vegetales d'une plante produisant des isoflavonoides
WO2003031622A1 (fr) * 2001-10-05 2003-04-17 Agriculture Victoria Services Pty Ltd Manipulation de la biosynthese des flavonoides chez les plantes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 10 September 1999 (1999-09-10), "Glycine max dihydroflavonol-4-reductase DFR1 mRNA, complete cds.", XP002311362, retrieved from EBI accession no. EM_PRO:AF167556 Database accession no. AF167556 *
MEYER P ET AL: "A NEW PETUNIA FLOWER COLOR GENERATED BY TRANSFORMATION OF A MUTANT WITH A MAIZE GENE", NATURE (LONDON), vol. 330, no. 6149, 1987, pages 677 - 678, XP002311408, ISSN: 0028-0836 *
SPARVOLI F ET AL: "CLONING AND MOLECULAR ANALYSIS OF STRUCTURAL GENES INVOLVED IN FLAVONOID AND STILBENE BIOSYNTHESIS IN GRAPE (VITIS VINIFERA L.)", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 24, no. 5, 1994, pages 743 - 755, XP001161123, ISSN: 0167-4412 *
TANAKA Y ET AL: "MOLECULAR CLONING AND CHARACTERIZATION OF ROSA HYBRIDA DIHYDROFLAVONOL 4-REDUCTASE GENE", PLANT AND CELL PHYSIOLOGY, JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS, XX, vol. 36, no. 6, September 1995 (1995-09-01), pages 1023 - 1031, XP001079999, ISSN: 0032-0781 *

Also Published As

Publication number Publication date
WO2005026368A2 (fr) 2005-03-24
CA2538314A1 (fr) 2005-03-24
EP1668138A2 (fr) 2006-06-14

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