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WO2005003778A3 - Procede d'identification d'une molecule synthetique ayant une affinite pour une cible - Google Patents

Procede d'identification d'une molecule synthetique ayant une affinite pour une cible Download PDF

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Publication number
WO2005003778A3
WO2005003778A3 PCT/DK2004/000466 DK2004000466W WO2005003778A3 WO 2005003778 A3 WO2005003778 A3 WO 2005003778A3 DK 2004000466 W DK2004000466 W DK 2004000466W WO 2005003778 A3 WO2005003778 A3 WO 2005003778A3
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WO
WIPO (PCT)
Prior art keywords
target
affinity towards
identifying
library
molecule
Prior art date
Application number
PCT/DK2004/000466
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English (en)
Other versions
WO2005003778A2 (fr
Inventor
Per-Ola Freskgaard
Mikkel Dybro Lundorf
Soeren Nyboe Jakobsen
Original Assignee
Nuevolution As
Per-Ola Freskgaard
Mikkel Dybro Lundorf
Soeren Nyboe Jakobsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nuevolution As, Per-Ola Freskgaard, Mikkel Dybro Lundorf, Soeren Nyboe Jakobsen filed Critical Nuevolution As
Publication of WO2005003778A2 publication Critical patent/WO2005003778A2/fr
Publication of WO2005003778A3 publication Critical patent/WO2005003778A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

L'invention concerne un procédé d'identification d'une molécule synthétique ayant une affinité pour une cible. Le procédé comprend les étapes consistant à utiliser une banque de complexes bifonctionnels renfermant individuellement une molécule synthétique fixée sur un identificateur codant ladite molécule; à soumettre, dans des conditions de liaison, la banque de complexes bifonctionnels à une cible; à éliminer les éléments non liants de la banque; à séparer les identificateurs de complexes comprenant des molécules synthétiques ayant une affinité pour la cible; et à décoder les identificateurs, de manière à établir l'identité de la molécule.
PCT/DK2004/000466 2003-07-02 2004-06-30 Procede d'identification d'une molecule synthetique ayant une affinite pour une cible WO2005003778A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48389903P 2003-07-02 2003-07-02
DKPA200301003 2003-07-02
DKPA200301003 2003-07-02
US60/483,899 2003-07-02

Publications (2)

Publication Number Publication Date
WO2005003778A2 WO2005003778A2 (fr) 2005-01-13
WO2005003778A3 true WO2005003778A3 (fr) 2005-04-14

Family

ID=33566213

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000466 WO2005003778A2 (fr) 2003-07-02 2004-06-30 Procede d'identification d'une molecule synthetique ayant une affinite pour une cible

Country Status (1)

Country Link
WO (1) WO2005003778A2 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1539980B1 (fr) 2002-08-01 2016-02-17 Nuevolution A/S Synthese en plusieurs etapes de molecules synthetisees
EP1608748B1 (fr) 2003-03-20 2009-03-04 Nuevolution A/S Codage par ligature de petites molecules
WO2005026387A1 (fr) 2003-09-18 2005-03-24 Nuevolution A/S Procede permettant d'obtenir des informations structurelles concernant une molecule codee et procede permettant de selectionner des composes
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
US7972992B2 (en) 2003-12-17 2011-07-05 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
EP1730277B1 (fr) 2004-03-22 2009-10-28 Nuevolution A/S Codage par ligature utilisant des oligonucleotides motifs structuraux
EP1774033A4 (fr) * 2004-06-10 2008-04-23 Perkinelmer Las Inc Detection d'analysat par essais a multiplexage
JP2009513135A (ja) 2005-10-28 2009-04-02 プリーシス・ファーマシューティカルズ・インコーポレイテッド エンコードされたライブラリーを用いて興味のある化合物を同定する方法
CA2832672A1 (fr) 2010-04-16 2011-10-20 Nuevolution A/S Complexes bifonctionnels et procedes de fabrication et d'utilisation de tels complexes
US20180340174A1 (en) 2014-11-11 2018-11-29 Nanocore Aps Method for identification of molecules with desired characteristics
US11186836B2 (en) 2016-06-16 2021-11-30 Haystack Sciences Corporation Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
WO2018204420A1 (fr) 2017-05-02 2018-11-08 Haystack Sciences Corporation Molécules de vérification de synthèse combinatoire dirigée par oligonucléotides et procédés de fabrication et d'utilisation associés

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006121A1 (fr) * 1991-09-18 1993-04-01 Affymax Technologies N.V. Procede de synthese de diverses collections d'oligomeres
WO1995004160A1 (fr) * 1993-07-30 1995-02-09 Isis Innovation Limited Reactif de marquage et methode de dosage
WO1995028640A1 (fr) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Bibliotheques complexes combinatoires de substances chimiques a codage par etiquettes
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1999060007A2 (fr) * 1998-05-15 1999-11-25 Isis Innovation Limited Reactif et procede
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
WO2002074929A2 (fr) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolution d'une nouvelle fonction moleculaire
WO2003002767A1 (fr) * 2001-06-29 2003-01-09 Agilent Technologies, Inc. Procede de sequençage d'adn au moyen d'etiquettes clivables

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993006121A1 (fr) * 1991-09-18 1993-04-01 Affymax Technologies N.V. Procede de synthese de diverses collections d'oligomeres
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1995004160A1 (fr) * 1993-07-30 1995-02-09 Isis Innovation Limited Reactif de marquage et methode de dosage
WO1995028640A1 (fr) * 1994-04-13 1995-10-26 The Trustees Of Columbia University In The City Of New York Bibliotheques complexes combinatoires de substances chimiques a codage par etiquettes
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
WO1999060007A2 (fr) * 1998-05-15 1999-11-25 Isis Innovation Limited Reactif et procede
WO2002074929A2 (fr) * 2001-03-19 2002-09-26 President And Fellows Of Harvard College Evolution d'une nouvelle fonction moleculaire
WO2003002767A1 (fr) * 2001-06-29 2003-01-09 Agilent Technologies, Inc. Procede de sequençage d'adn au moyen d'etiquettes clivables

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRENNER S ET AL: "ENCODED COMBINATORIAL CHEMISTRY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, no. 12, 1 June 1992 (1992-06-01), pages 5381 - 5383, XP000647936, ISSN: 0027-8424 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US7442160B2 (en) 2001-03-19 2008-10-28 President And Fellows Of Harvard College Evolving new molecular function
US7557068B2 (en) 2001-03-19 2009-07-07 President And Fellows Of Harvard College Evolving new molecular function
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8722583B2 (en) 2002-10-30 2014-05-13 Nuevolution A/S Method for selecting a chemical entity from a tagged library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9284600B2 (en) 2002-10-30 2016-03-15 Neuvolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

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Publication number Publication date
WO2005003778A2 (fr) 2005-01-13

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