+

WO2005093069A3 - Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells - Google Patents

Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells Download PDF

Info

Publication number
WO2005093069A3
WO2005093069A3 PCT/GB2005/001055 GB2005001055W WO2005093069A3 WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3 GB 2005001055 W GB2005001055 W GB 2005001055W WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3
Authority
WO
WIPO (PCT)
Prior art keywords
mersacidin
variants
cell
production
sigh
Prior art date
Application number
PCT/GB2005/001055
Other languages
French (fr)
Other versions
WO2005093069A2 (en
Inventor
Michael John Dawson
Bargallo Jesus Cortes
Brian Arthur Michael Rudd
Steven Boakes
Gabriele Bierbaum
Anja Hoffmann
Stephanie Schmitz
Original Assignee
Novacta Biosystems Ltd
Michael John Dawson
Bargallo Jesus Cortes
Brian Arthur Michael Rudd
Steven Boakes
Gabriele Bierbaum
Anja Hoffmann
Stephanie Schmitz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novacta Biosystems Ltd, Michael John Dawson, Bargallo Jesus Cortes, Brian Arthur Michael Rudd, Steven Boakes, Gabriele Bierbaum, Anja Hoffmann, Stephanie Schmitz filed Critical Novacta Biosystems Ltd
Priority to AU2005225595A priority Critical patent/AU2005225595A1/en
Priority to EP05729379A priority patent/EP1794298A2/en
Publication of WO2005093069A2 publication Critical patent/WO2005093069A2/en
Publication of WO2005093069A3 publication Critical patent/WO2005093069A3/en
Priority to US11/526,225 priority patent/US7592308B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a negative MrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants mersacidin F3W, mersacidin G8A and mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.
PCT/GB2005/001055 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells WO2005093069A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2005225595A AU2005225595A1 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in SigH and/or MrsA negative bacillus host cells
EP05729379A EP1794298A2 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells
US11/526,225 US7592308B2 (en) 2004-03-26 2006-09-25 F3W variants of the lantibiotic mersacidin and its use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0406870A GB0406870D0 (en) 2004-03-26 2004-03-26 Improvements relating to the production of lantibiotics
GB0406870.6 2004-03-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/526,225 Continuation-In-Part US7592308B2 (en) 2004-03-26 2006-09-25 F3W variants of the lantibiotic mersacidin and its use

Publications (2)

Publication Number Publication Date
WO2005093069A2 WO2005093069A2 (en) 2005-10-06
WO2005093069A3 true WO2005093069A3 (en) 2005-12-08

Family

ID=32188789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/001055 WO2005093069A2 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells

Country Status (4)

Country Link
EP (1) EP1794298A2 (en)
AU (1) AU2005225595A1 (en)
GB (1) GB0406870D0 (en)
WO (1) WO2005093069A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7592308B2 (en) 2004-03-26 2009-09-22 Novacta Biosystems Limited F3W variants of the lantibiotic mersacidin and its use
WO2007036706A1 (en) * 2005-09-27 2007-04-05 Novacta Biosystems Limited Variants of the lantibiotic mersacidin and their use
GB0600928D0 (en) 2006-01-17 2006-02-22 Novacta Biosystems Ltd Improvements relating to lantibiotics
GB0714029D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd Lantibiotic-based compounds having antimicrobial activity
GB0714030D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd The use of type-B lantibiotic-based compounds having antimicrobial activity
AU2010205472B2 (en) 2009-01-14 2013-03-14 Novacta Biosystems Limited Deoxyactagardine derivatives
US8729031B2 (en) 2009-02-04 2014-05-20 Novacta Biosystems Limited Compounds
GB201001688D0 (en) 2010-02-02 2010-03-17 Novacta Biosystems Ltd Compounds
GB201013513D0 (en) 2010-08-11 2010-09-22 Novacta Biosystems Ltd Formulations

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5667991A (en) * 1994-09-12 1997-09-16 Hoechst Aktiengesellschaft Recombinant mersacidin and a method for production
WO2003099862A1 (en) * 2002-05-24 2003-12-04 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3710795A (en) 1970-09-29 1973-01-16 Alza Corp Drug-delivery device with stretched, rate-controlling membrane
IN167138B (en) 1988-08-17 1990-09-01 Hoechst India
WO2007036706A1 (en) * 2005-09-27 2007-04-05 Novacta Biosystems Limited Variants of the lantibiotic mersacidin and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5667991A (en) * 1994-09-12 1997-09-16 Hoechst Aktiengesellschaft Recombinant mersacidin and a method for production
WO2003099862A1 (en) * 2002-05-24 2003-12-04 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALTENA KARSTEN ET AL: "Biosynthesis of the lantibiotic mersacidin: Organization of a type B lantibiotic gene cluster", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 66, no. 6, June 2000 (2000-06-01), pages 2565 - 2571, XP002209341, ISSN: 0099-2240 *
BRITTON ROBERT A ET AL: "Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis", JOURNAL OF BACTERIOLOGY, vol. 184, no. 17, September 2002 (2002-09-01), pages 4881 - 4890, XP002341472, ISSN: 0021-9193 *
GUDER A ET AL: "ROLE OF THE SINGLE REGULATOR MRSR1 AND THE TWO-COMPONENT SYSTEM MRSR2/K2 IN THE REGULATION OF MERSACIDIN PRODUCTION AND IMMUNITY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 68, no. 1, January 2002 (2002-01-01), pages 106 - 113, XP001206740, ISSN: 0099-2240 *
See also references of EP1794298A2 *
SZEKAT C ET AL: "CONSTRUCTION OF AN EXPRESSION SYSTEM FOR SITE-DIRECTED MUTAGENESIS OF THE LANTIBIOTIC MERSACIDIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 69, no. 7, July 2003 (2003-07-01), pages 3777 - 3783, XP001206733, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
EP1794298A2 (en) 2007-06-13
GB0406870D0 (en) 2004-04-28
WO2005093069A2 (en) 2005-10-06
AU2005225595A1 (en) 2005-10-06

Similar Documents

Publication Publication Date Title
WO2003066818A3 (en) Secretion, transcription and sporulation genes in bacillus clausii
Yang et al. Generation of an artificial double promoter for protein expression in Bacillus subtilis through a promoter trap system
WO2009011258A1 (en) Method for maintaining foreign gene in cell stably
WO2005069762A3 (en) Bacillus licheniformis chromosome
WO2007140339A8 (en) Production of isoprenoids
MX2008000200A (en) Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof.
PL2169066T3 (en) Promoter nucleic acid derived from Corynebacterium genus bacteria, expression cassette comprising the promoter and vector comprising the cassette, host cell comprising the vector and method for expressing a gene using the cell
WO2003054163A3 (en) Methods for producing hyaluronan in a recombinant host cell
WO2003100070A3 (en) The frog prince, a transposon vector for gene transfer in vertebrates
WO2010006312A3 (en) Methods and compositions for producing carbon-based products in microorganisms
WO2009042070A3 (en) Production of isoprenoids
AU2002311989A1 (en) Methods and materials for the production of organic products in cells of $i(candida) species
WO2001092539A3 (en) Method for the production of p-hydroxybenzoate in species of pseudomonas and agrobacterium
WO2007005837A3 (en) Inducible expression vectors and methods of use thereof
WO2005033094A3 (en) Methods for the production of 3-methylamino-1-(thiene-2-yl)-propane-1-ol
Vertès et al. Manipulating corynebacteria, from individual genes to chromosomes
WO2008020333A3 (en) Nucleic acid encoding n-methylputrescine oxidase and uses thereof
TNSN07090A1 (en) Novel laccase enzyme and use thereof
WO2005093069A3 (en) Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells
EP2434018A3 (en) A novel beta-galactoside-alpha2, 3-sialyltransferase, a gene encoding thereof, and a method for producing thereof
WO2005087802A3 (en) Process for producing polypeptides
ATE486935T1 (en) MICROORGANISM STRAIN FOR THE PRODUCTION OF RECOMBINANT PROTEINS
Gomes et al. Two‐dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria
TW200638944A (en) Recombinant enterovirus 71 neutralizing antibodies and use thereof
Firsova et al. Functionality of the xoxF gene in Methylobacterium dichloromethanicum DM4

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 11526225

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 2005225595

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2005729379

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2005225595

Country of ref document: AU

Date of ref document: 20050321

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2005225595

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 11526225

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2005729379

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载