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WO2005093069A3 - Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells - Google Patents

Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells Download PDF

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Publication number
WO2005093069A3
WO2005093069A3 PCT/GB2005/001055 GB2005001055W WO2005093069A3 WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3 GB 2005001055 W GB2005001055 W GB 2005001055W WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3
Authority
WO
WIPO (PCT)
Prior art keywords
mersacidin
variants
cell
production
sigh
Prior art date
Application number
PCT/GB2005/001055
Other languages
French (fr)
Other versions
WO2005093069A2 (en
Inventor
Michael John Dawson
Bargallo Jesus Cortes
Brian Arthur Michael Rudd
Steven Boakes
Gabriele Bierbaum
Anja Hoffmann
Stephanie Schmitz
Original Assignee
Novacta Biosystems Ltd
Michael John Dawson
Bargallo Jesus Cortes
Brian Arthur Michael Rudd
Steven Boakes
Gabriele Bierbaum
Anja Hoffmann
Stephanie Schmitz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novacta Biosystems Ltd, Michael John Dawson, Bargallo Jesus Cortes, Brian Arthur Michael Rudd, Steven Boakes, Gabriele Bierbaum, Anja Hoffmann, Stephanie Schmitz filed Critical Novacta Biosystems Ltd
Priority to AU2005225595A priority Critical patent/AU2005225595A1/en
Priority to EP05729379A priority patent/EP1794298A2/en
Publication of WO2005093069A2 publication Critical patent/WO2005093069A2/en
Publication of WO2005093069A3 publication Critical patent/WO2005093069A3/en
Priority to US11/526,225 priority patent/US7592308B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a negative MrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants mersacidin F3W, mersacidin G8A and mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.
PCT/GB2005/001055 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells WO2005093069A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2005225595A AU2005225595A1 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in SigH and/or MrsA negative bacillus host cells
EP05729379A EP1794298A2 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells
US11/526,225 US7592308B2 (en) 2004-03-26 2006-09-25 F3W variants of the lantibiotic mersacidin and its use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0406870A GB0406870D0 (en) 2004-03-26 2004-03-26 Improvements relating to the production of lantibiotics
GB0406870.6 2004-03-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/526,225 Continuation-In-Part US7592308B2 (en) 2004-03-26 2006-09-25 F3W variants of the lantibiotic mersacidin and its use

Publications (2)

Publication Number Publication Date
WO2005093069A2 WO2005093069A2 (en) 2005-10-06
WO2005093069A3 true WO2005093069A3 (en) 2005-12-08

Family

ID=32188789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/001055 WO2005093069A2 (en) 2004-03-26 2005-03-21 Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells

Country Status (4)

Country Link
EP (1) EP1794298A2 (en)
AU (1) AU2005225595A1 (en)
GB (1) GB0406870D0 (en)
WO (1) WO2005093069A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7592308B2 (en) 2004-03-26 2009-09-22 Novacta Biosystems Limited F3W variants of the lantibiotic mersacidin and its use
EP1928900A1 (en) * 2005-09-27 2008-06-11 Novacta Biosystems Limited Variants of the lantibiotic mersacidin and their use
GB0600928D0 (en) 2006-01-17 2006-02-22 Novacta Biosystems Ltd Improvements relating to lantibiotics
GB0714029D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd Lantibiotic-based compounds having antimicrobial activity
GB0714030D0 (en) 2007-07-18 2007-08-29 Novacta Biosystems Ltd The use of type-B lantibiotic-based compounds having antimicrobial activity
US8283371B2 (en) 2009-01-14 2012-10-09 Novacta Biosystems Limited Compounds
MX2011008043A (en) 2009-02-04 2011-08-17 Novacta Biosystems Ltd Actagardine derivatives.
GB201001688D0 (en) 2010-02-02 2010-03-17 Novacta Biosystems Ltd Compounds
GB201013513D0 (en) 2010-08-11 2010-09-22 Novacta Biosystems Ltd Formulations

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5667991A (en) * 1994-09-12 1997-09-16 Hoechst Aktiengesellschaft Recombinant mersacidin and a method for production
WO2003099862A1 (en) * 2002-05-24 2003-12-04 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3710795A (en) 1970-09-29 1973-01-16 Alza Corp Drug-delivery device with stretched, rate-controlling membrane
IN167138B (en) 1988-08-17 1990-09-01 Hoechst India
EP1928900A1 (en) * 2005-09-27 2008-06-11 Novacta Biosystems Limited Variants of the lantibiotic mersacidin and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5667991A (en) * 1994-09-12 1997-09-16 Hoechst Aktiengesellschaft Recombinant mersacidin and a method for production
WO2003099862A1 (en) * 2002-05-24 2003-12-04 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALTENA KARSTEN ET AL: "Biosynthesis of the lantibiotic mersacidin: Organization of a type B lantibiotic gene cluster", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 66, no. 6, June 2000 (2000-06-01), pages 2565 - 2571, XP002209341, ISSN: 0099-2240 *
BRITTON ROBERT A ET AL: "Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis", JOURNAL OF BACTERIOLOGY, vol. 184, no. 17, September 2002 (2002-09-01), pages 4881 - 4890, XP002341472, ISSN: 0021-9193 *
GUDER A ET AL: "ROLE OF THE SINGLE REGULATOR MRSR1 AND THE TWO-COMPONENT SYSTEM MRSR2/K2 IN THE REGULATION OF MERSACIDIN PRODUCTION AND IMMUNITY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 68, no. 1, January 2002 (2002-01-01), pages 106 - 113, XP001206740, ISSN: 0099-2240 *
See also references of EP1794298A2 *
SZEKAT C ET AL: "CONSTRUCTION OF AN EXPRESSION SYSTEM FOR SITE-DIRECTED MUTAGENESIS OF THE LANTIBIOTIC MERSACIDIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 69, no. 7, July 2003 (2003-07-01), pages 3777 - 3783, XP001206733, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
EP1794298A2 (en) 2007-06-13
GB0406870D0 (en) 2004-04-28
AU2005225595A1 (en) 2005-10-06
WO2005093069A2 (en) 2005-10-06

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