WO2005093069A3 - Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells - Google Patents
Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells Download PDFInfo
- Publication number
- WO2005093069A3 WO2005093069A3 PCT/GB2005/001055 GB2005001055W WO2005093069A3 WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3 GB 2005001055 W GB2005001055 W GB 2005001055W WO 2005093069 A3 WO2005093069 A3 WO 2005093069A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mersacidin
- variants
- cell
- production
- sigh
- Prior art date
Links
- 108010067215 mersacidin Proteins 0.000 title abstract 7
- JSWKNDSDVHJUKY-CYGWNLPQSA-N mersacidin Chemical compound C([C@@H](C(=O)N[C@@H]1[C@H](C)SC[C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)CNC(=O)CNC(=O)CNC(=O)[C@@H]2CCCN2C(=O)[C@H](CC(C)C)NC1=O)C(=O)N[C@@H]1[C@H](C)SC[C@H]2C(=O)N[C@H](C(N/C=C/S[C@@H](C)C(NC(=O)[C@H](CC(C)C)NC1=O)C(=O)NC(=C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)[C@H](C)CC)NC(=O)[C@H]1[C@@H](SC[C@H](N)C(=O)N1)C)C1=CC=CC=C1 JSWKNDSDVHJUKY-CYGWNLPQSA-N 0.000 title abstract 7
- 241000193830 Bacillus <bacterium> Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 abstract 4
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 abstract 1
- 101710187725 Lantibiotic mersacidin Proteins 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 238000004520 electroporation Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2005225595A AU2005225595A1 (en) | 2004-03-26 | 2005-03-21 | Production of mersacidin and its variants in SigH and/or MrsA negative bacillus host cells |
EP05729379A EP1794298A2 (en) | 2004-03-26 | 2005-03-21 | Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells |
US11/526,225 US7592308B2 (en) | 2004-03-26 | 2006-09-25 | F3W variants of the lantibiotic mersacidin and its use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0406870A GB0406870D0 (en) | 2004-03-26 | 2004-03-26 | Improvements relating to the production of lantibiotics |
GB0406870.6 | 2004-03-26 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/526,225 Continuation-In-Part US7592308B2 (en) | 2004-03-26 | 2006-09-25 | F3W variants of the lantibiotic mersacidin and its use |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005093069A2 WO2005093069A2 (en) | 2005-10-06 |
WO2005093069A3 true WO2005093069A3 (en) | 2005-12-08 |
Family
ID=32188789
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2005/001055 WO2005093069A2 (en) | 2004-03-26 | 2005-03-21 | Production of mersacidin and its variants in sigh and/or mrsa negative bacillus host cells |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1794298A2 (en) |
AU (1) | AU2005225595A1 (en) |
GB (1) | GB0406870D0 (en) |
WO (1) | WO2005093069A2 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7592308B2 (en) | 2004-03-26 | 2009-09-22 | Novacta Biosystems Limited | F3W variants of the lantibiotic mersacidin and its use |
EP1928900A1 (en) * | 2005-09-27 | 2008-06-11 | Novacta Biosystems Limited | Variants of the lantibiotic mersacidin and their use |
GB0600928D0 (en) | 2006-01-17 | 2006-02-22 | Novacta Biosystems Ltd | Improvements relating to lantibiotics |
GB0714029D0 (en) | 2007-07-18 | 2007-08-29 | Novacta Biosystems Ltd | Lantibiotic-based compounds having antimicrobial activity |
GB0714030D0 (en) | 2007-07-18 | 2007-08-29 | Novacta Biosystems Ltd | The use of type-B lantibiotic-based compounds having antimicrobial activity |
US8283371B2 (en) | 2009-01-14 | 2012-10-09 | Novacta Biosystems Limited | Compounds |
MX2011008043A (en) | 2009-02-04 | 2011-08-17 | Novacta Biosystems Ltd | Actagardine derivatives. |
GB201001688D0 (en) | 2010-02-02 | 2010-03-17 | Novacta Biosystems Ltd | Compounds |
GB201013513D0 (en) | 2010-08-11 | 2010-09-22 | Novacta Biosystems Ltd | Formulations |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5667991A (en) * | 1994-09-12 | 1997-09-16 | Hoechst Aktiengesellschaft | Recombinant mersacidin and a method for production |
WO2003099862A1 (en) * | 2002-05-24 | 2003-12-04 | Applied Nanosystems B.V. | Export and modification of (poly)peptides in the lantibiotic way |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3710795A (en) | 1970-09-29 | 1973-01-16 | Alza Corp | Drug-delivery device with stretched, rate-controlling membrane |
IN167138B (en) | 1988-08-17 | 1990-09-01 | Hoechst India | |
EP1928900A1 (en) * | 2005-09-27 | 2008-06-11 | Novacta Biosystems Limited | Variants of the lantibiotic mersacidin and their use |
-
2004
- 2004-03-26 GB GB0406870A patent/GB0406870D0/en not_active Ceased
-
2005
- 2005-03-21 WO PCT/GB2005/001055 patent/WO2005093069A2/en active Application Filing
- 2005-03-21 AU AU2005225595A patent/AU2005225595A1/en not_active Abandoned
- 2005-03-21 EP EP05729379A patent/EP1794298A2/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5667991A (en) * | 1994-09-12 | 1997-09-16 | Hoechst Aktiengesellschaft | Recombinant mersacidin and a method for production |
WO2003099862A1 (en) * | 2002-05-24 | 2003-12-04 | Applied Nanosystems B.V. | Export and modification of (poly)peptides in the lantibiotic way |
Non-Patent Citations (5)
Title |
---|
ALTENA KARSTEN ET AL: "Biosynthesis of the lantibiotic mersacidin: Organization of a type B lantibiotic gene cluster", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 66, no. 6, June 2000 (2000-06-01), pages 2565 - 2571, XP002209341, ISSN: 0099-2240 * |
BRITTON ROBERT A ET AL: "Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis", JOURNAL OF BACTERIOLOGY, vol. 184, no. 17, September 2002 (2002-09-01), pages 4881 - 4890, XP002341472, ISSN: 0021-9193 * |
GUDER A ET AL: "ROLE OF THE SINGLE REGULATOR MRSR1 AND THE TWO-COMPONENT SYSTEM MRSR2/K2 IN THE REGULATION OF MERSACIDIN PRODUCTION AND IMMUNITY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 68, no. 1, January 2002 (2002-01-01), pages 106 - 113, XP001206740, ISSN: 0099-2240 * |
See also references of EP1794298A2 * |
SZEKAT C ET AL: "CONSTRUCTION OF AN EXPRESSION SYSTEM FOR SITE-DIRECTED MUTAGENESIS OF THE LANTIBIOTIC MERSACIDIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 69, no. 7, July 2003 (2003-07-01), pages 3777 - 3783, XP001206733, ISSN: 0099-2240 * |
Also Published As
Publication number | Publication date |
---|---|
EP1794298A2 (en) | 2007-06-13 |
GB0406870D0 (en) | 2004-04-28 |
AU2005225595A1 (en) | 2005-10-06 |
WO2005093069A2 (en) | 2005-10-06 |
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