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WO2005080579A3 - Taxadiene biosynthesis - Google Patents

Taxadiene biosynthesis Download PDF

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Publication number
WO2005080579A3
WO2005080579A3 PCT/GB2005/000578 GB2005000578W WO2005080579A3 WO 2005080579 A3 WO2005080579 A3 WO 2005080579A3 GB 2005000578 W GB2005000578 W GB 2005000578W WO 2005080579 A3 WO2005080579 A3 WO 2005080579A3
Authority
WO
WIPO (PCT)
Prior art keywords
taxadiene
plants
biosynthesis
ggdp
metabolism
Prior art date
Application number
PCT/GB2005/000578
Other languages
French (fr)
Other versions
WO2005080579A2 (en
Inventor
Rupert Fray
Original Assignee
Plant Bioscience Ltd
Rupert Fray
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd, Rupert Fray filed Critical Plant Bioscience Ltd
Publication of WO2005080579A2 publication Critical patent/WO2005080579A2/en
Publication of WO2005080579A3 publication Critical patent/WO2005080579A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Epoxy Compounds (AREA)

Abstract

The present invention relates to the finding that plants which are deficient in geranylgeranyl diphosphate (GGDP) metabolism, in particular tomato and other carotenoid synthesising plants, are useful in the biosynthesis of taxa-4, 11-diene (taxadiene) and other paclitaxel intermediates. Methods and means of producing taxadiene and other paclitaxel intermediates using plants deficient in GGDP metabolism are provided.
PCT/GB2005/000578 2004-02-20 2005-02-18 Taxadiene biosynthesis WO2005080579A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0403847.7 2004-02-20
GBGB0403847.7A GB0403847D0 (en) 2004-02-20 2004-02-20 Methods and means

Publications (2)

Publication Number Publication Date
WO2005080579A2 WO2005080579A2 (en) 2005-09-01
WO2005080579A3 true WO2005080579A3 (en) 2006-03-30

Family

ID=32040111

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/000578 WO2005080579A2 (en) 2004-02-20 2005-02-18 Taxadiene biosynthesis

Country Status (2)

Country Link
GB (1) GB0403847D0 (en)
WO (1) WO2005080579A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7906301B2 (en) 2005-05-25 2011-03-15 Expression Pathology, Inc. Multiplex method for increased proteomic coverage from histopathologically processed biological samples, tissues cells
CN104140689B (en) * 2014-07-14 2016-02-17 红豆集团无锡南国企业有限公司 The method of natural pigment is extracted from Ramulus et folium taxi cuspidatae pharmacy residue
CN104450312B (en) * 2014-11-27 2018-02-06 南京信息职业技术学院 Enzyme soap and preparation method thereof
CN104450316A (en) * 2014-12-10 2015-03-25 江西省喜果绿化有限公司 Taxus chinensis soap for dry skin
CN111748547B (en) 2020-07-07 2021-12-03 中国农业科学院深圳农业基因组研究所 Taxadiene synthase TcTS2, coding nucleotide sequence and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038571A1 (en) * 1996-04-15 1997-10-23 Washington State University Research Foundation Compositions and methods for taxol biosynthesis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038571A1 (en) * 1996-04-15 1997-10-23 Washington State University Research Foundation Compositions and methods for taxol biosynthesis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRAMLEY PETER, TEULIERES CHANTAL, BLAIN IAN, BIRD COLIN SCHUCH WOLFGANG: "Biochemical characterisation of transgenic tomato plants in which carotenoid synthesis has been inhibited through the expression of antisense RNA to pTOM5", THE PLANT JOURNAL, vol. 2, no. 3, 1992, pages 343 - 349, XP002362541 *
HUANG Q ET AL: "Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol.", BIOORGANIC & MEDICINAL CHEMISTRY. SEP 2001, vol. 9, no. 9, September 2001 (2001-09-01), pages 2237 - 2242, XP002362540, ISSN: 0968-0896 *
WILDUNG M R ET AL: "CDNA CLONE FOR TAXADIENE SYNTHASE, THE DITERPENE CYCLASE THAT CATALYZES THE COMMITTED STEP OF TAXOL BIOSYNTHESIS", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 271, no. 16, 19 April 1996 (1996-04-19), pages 9201 - 9204, XP000938117, ISSN: 0021-9258 *
WILLIAMS DAVID C ET AL: "Heterologous expression and characterization of a "pseudomature" form of taxadiene synthase involved in paclitaxel (taxol) biosynthesis and evaluation of a potential intermediate and inhibitors of the multistep diterpene cyclization reaction", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 379, no. 1, 1 July 2000 (2000-07-01), pages 137 - 146, XP002362542, ISSN: 0003-9861 *

Also Published As

Publication number Publication date
GB0403847D0 (en) 2004-03-24
WO2005080579A2 (en) 2005-09-01

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