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WO2004113546A3 - Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, $g(b),3-glucanase et la proteine assimilee a la thautamine et leur utilisation - Google Patents

Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, $g(b),3-glucanase et la proteine assimilee a la thautamine et leur utilisation Download PDF

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Publication number
WO2004113546A3
WO2004113546A3 PCT/PT2004/000015 PT2004000015W WO2004113546A3 WO 2004113546 A3 WO2004113546 A3 WO 2004113546A3 PT 2004000015 W PT2004000015 W PT 2004000015W WO 2004113546 A3 WO2004113546 A3 WO 2004113546A3
Authority
WO
WIPO (PCT)
Prior art keywords
thaumatin
cystatin
glucanase
protein
nucleotide sequences
Prior art date
Application number
PCT/PT2004/000015
Other languages
English (en)
Other versions
WO2004113546A2 (fr
Inventor
Serrazina Susana Mari Traquete
Fonseca Sandra Cristina Matias
Pais Maria Salome Soares
Aladje Balde
Original Assignee
Castania Sociedade Agroflorest
Serrazina Susana Mari Traquete
Fonseca Sandra Cristina Matias
Pais Maria Salome Soares
Aladje Balde
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Castania Sociedade Agroflorest, Serrazina Susana Mari Traquete, Fonseca Sandra Cristina Matias, Pais Maria Salome Soares, Aladje Balde filed Critical Castania Sociedade Agroflorest
Priority to US10/562,144 priority Critical patent/US20070101455A1/en
Priority to CA002570502A priority patent/CA2570502A1/fr
Priority to EP04748885A priority patent/EP1646723A2/fr
Publication of WO2004113546A2 publication Critical patent/WO2004113546A2/fr
Publication of WO2004113546A3 publication Critical patent/WO2004113546A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/99—Other intramolecular oxidoreductases (5.3.99)
    • C12Y503/99006—Allene-oxide cyclase (5.3.99.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention a trait à des séquences nucléotidiques isolées et purifiées qui sont exprimées de manière différente lors d'infection du châtaignier par le champignon pathogène Phytophthtora cinnamomi Rands. Les gènes isolés peuvent être introduits dans des cassettes d'expression et clonés dans un vecteur d'expression qui peut être utilisé pour la transformation d'une cellule hôte par des procédés de sélection. Des plantes transgéniques peuvent être régénérées à partir de cellules végétales transformées par des techniques de culture in vitro. Les séquences nucléotidiques de l'invention codent pour des protéines qui sont décrites comme ayant une action efficace dans la résistance des plantes aux champignons pathogènes. Lors de leur utilisation dans l'orientation de sens elles peuvent retarder et même empêcher l'infection des plantes, procurant d'importants avantages aux producteurs de châtaigniers, de chênes-lièges ou d'autres espèces d'arbres ligneux.
PCT/PT2004/000015 2003-06-26 2004-06-25 Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, $g(b),3-glucanase et la proteine assimilee a la thautamine et leur utilisation WO2004113546A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/562,144 US20070101455A1 (en) 2003-06-26 2004-06-25 Castanea sativa mill. genes codifying for allene oxide cyclase, cystatin, beta-1, 3-glucanase and thaumatin-like protein and their use
CA002570502A CA2570502A1 (fr) 2003-06-26 2004-06-25 Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, beta-1,3-glucanase et la proteine assimilee a la thautamine et leur utilisation
EP04748885A EP1646723A2 (fr) 2003-06-26 2004-06-25 Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, beta-1,3-glucanase et la proteine assimilee a la thautamine et leur utilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT102979A PT102979A (pt) 2003-06-26 2003-06-26 Genes codificantes de oxido de aleno ciclase (aoc), cistatina, beta-1,3-glucanasee de proteina taumatin-like isolados de castanheiro europeu (castanea sativa mill.)
PT102979S 2003-06-26

Publications (2)

Publication Number Publication Date
WO2004113546A2 WO2004113546A2 (fr) 2004-12-29
WO2004113546A3 true WO2004113546A3 (fr) 2005-04-14

Family

ID=33536791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/PT2004/000015 WO2004113546A2 (fr) 2003-06-26 2004-06-25 Genes de castanea sativa mill. codant pour l'allene oxyde cyclase, la cystatine, $g(b),3-glucanase et la proteine assimilee a la thautamine et leur utilisation

Country Status (6)

Country Link
US (1) US20070101455A1 (fr)
EP (1) EP1646723A2 (fr)
CN (1) CN1842599A (fr)
CA (1) CA2570502A1 (fr)
PT (1) PT102979A (fr)
WO (1) WO2004113546A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105145340B (zh) * 2015-09-08 2017-07-21 北京市农林科学院 短雄花序板栗品种的分子育种方法
CN107488669B (zh) * 2017-09-25 2021-06-25 南开大学 花椰菜BoTLP1基因的编码序列及其在培育耐盐抗旱转基因植物中的应用
CN119351423B (zh) * 2024-12-26 2025-03-07 北京林业大学 一种与板栗疫病菌致病性相关的基因CpSge1及其dsRNA和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000001804A2 (fr) * 1998-07-03 2000-01-13 Unilever N.V. Sequence nucleotidique
WO2001057224A2 (fr) * 2000-02-02 2001-08-09 Institut Für Pflanzenbiochemie Gene d'allene oxyde cyclase et son utilisation pour la preparation d'acide jasmonique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2291987B (en) * 1993-03-26 1997-04-02 Komatsu Mfg Co Ltd Controller for hydraulic drive machine
DE19904616A1 (de) * 1999-02-05 2000-08-10 Mannesmann Rexroth Ag Steueranordnung für wenigstens zwei hydraulische Verbraucher und Druckdifferenzventil dafür
CN1306606A (zh) * 1999-05-28 2001-08-01 日立建机株式会社 泵容量控制装置和阀装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000001804A2 (fr) * 1998-07-03 2000-01-13 Unilever N.V. Sequence nucleotidique
WO2001057224A2 (fr) * 2000-02-02 2001-08-09 Institut Für Pflanzenbiochemie Gene d'allene oxyde cyclase et son utilisation pour la preparation d'acide jasmonique

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ARAI SOICHI ET AL: "Plant seed cystatins and their target enzymes of endogenous and exogenous origin", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 50, no. 22, 23 October 2002 (2002-10-23), pages 6612 - 6617, XP002316940, ISSN: 0021-8561 *
BEFFA R ET AL: "Pathogenesis-related functions of plant beta-1,3-glucanases investigated by antisense transformation -- a review", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 179, no. 1, 7 November 1996 (1996-11-07), pages 97 - 103, XP004071970, ISSN: 0378-1119 *
CHYE M-L ET AL: "BETA-1,3-GLUCANASE IS HIGHLY-EXPRESSED IN LATICIFERS OF HEVEA BRASILIENSIS", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 29, no. 2, 1995, pages 397 - 402, XP009011522, ISSN: 0167-4412 *
GARCIA-CASADO GLORIA ET AL: "Characterization of an apoplastic basic thaumatin-like protein from recalcitrant chestnut seeds", PHYSIOLOGIA PLANTARUM, vol. 110, no. 2, October 2000 (2000-10-01), pages 172 - 180, XP002304729, ISSN: 0031-9317 *
PERNAS M ET AL: "Biotic and abiotic stress can induce cystatin expression in chestnut", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 467, no. 2-3, 11 February 2000 (2000-02-11), pages 206 - 210, XP004260953, ISSN: 0014-5793 *
PERNAS MONICA ET AL: "A chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests", PLANT MOLECULAR BIOLOGY, vol. 38, no. 6, December 1998 (1998-12-01), pages 1235 - 1242, XP002316939, ISSN: 0167-4412 *
SCHAFLEITNER R ET AL: "Effect of virulent and hypovirulent Cryphonectria parasitica (Murr.) Barr on the intercellular pathogen related proteins and on total protein pattern of chestnut (Castanea sativa Mill.)", PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, vol. 51, no. 5, November 1997 (1997-11-01), pages 323 - 332, XP002304728, ISSN: 0885-5765 *
STINTZI A ET AL: "PLANT 'PATHOGENESIS-RELATED' PROTEINS AND THEIR ROLE IN DEFENSE AGAINST PATHOGENS", BIOCHIMIE, MASSON, PARIS, FR, vol. 75, no. 8, 1993, pages 687 - 706, XP009006230, ISSN: 0300-9084 *
ZIEGLER JOERG ET AL: "Molecular cloning of allene oxide cyclase: The enzyme establishing the stereochemistry of octadecanoids and jasmonates", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 275, no. 25, 23 June 2000 (2000-06-23), pages 19132 - 19138, XP002182669, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
PT102979A (pt) 2004-12-31
CN1842599A (zh) 2006-10-04
EP1646723A2 (fr) 2006-04-19
WO2004113546A2 (fr) 2004-12-29
US20070101455A1 (en) 2007-05-03
CA2570502A1 (fr) 2004-12-29

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