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WO2004080378A3 - Methodes et outils pour le criblage d’arn actifs in cellulo - Google Patents

Methodes et outils pour le criblage d’arn actifs in cellulo Download PDF

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Publication number
WO2004080378A3
WO2004080378A3 PCT/FR2004/000499 FR2004000499W WO2004080378A3 WO 2004080378 A3 WO2004080378 A3 WO 2004080378A3 FR 2004000499 W FR2004000499 W FR 2004000499W WO 2004080378 A3 WO2004080378 A3 WO 2004080378A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cellulo
active rna
tools
rna
Prior art date
Application number
PCT/FR2004/000499
Other languages
English (en)
Other versions
WO2004080378A2 (fr
Inventor
Sylvie Barcellini-Couget
Alain Doglio
Original Assignee
Univ Nice Sophia Antipolis
Sylvie Barcellini-Couget
Alain Doglio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nice Sophia Antipolis, Sylvie Barcellini-Couget, Alain Doglio filed Critical Univ Nice Sophia Antipolis
Priority to US10/547,489 priority Critical patent/US20070122798A1/en
Priority to EP04716629A priority patent/EP1599604A2/fr
Publication of WO2004080378A2 publication Critical patent/WO2004080378A2/fr
Publication of WO2004080378A3 publication Critical patent/WO2004080378A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des méthodes et compositions pour le criblage d'ARN actifs in cellulo. Elle concerne notamment des procédés de préparation et de sélection d'ARN aléatoires conférant à une cellule un phénotype désiré, des banques d'ARN aléatoires et leur production, ainsi que des populations cellulaires transformées par ces banques. Elle concerne également l'utilisation des ARN actifs identifiés à des fins de recherche ou thérapeutiques, pour induire un phénotype désiré dans une cellule in vitro, ex vivo ou in vivo, notamment dans des cellules humaines.
PCT/FR2004/000499 2003-03-04 2004-03-03 Methodes et outils pour le criblage d’arn actifs in cellulo WO2004080378A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/547,489 US20070122798A1 (en) 2003-03-04 2004-03-03 Methods and tools for screening active rna in cellulo
EP04716629A EP1599604A2 (fr) 2003-03-04 2004-03-03 Methodes et outils pour le criblage d'arn actifs in cellulo

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0302633A FR2852021B1 (fr) 2003-03-04 2003-03-04 Methodes et outils pour le criblage d'arn actifs in cellulo
FR03/02633 2003-03-04

Publications (2)

Publication Number Publication Date
WO2004080378A2 WO2004080378A2 (fr) 2004-09-23
WO2004080378A3 true WO2004080378A3 (fr) 2005-04-21

Family

ID=32865224

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/000499 WO2004080378A2 (fr) 2003-03-04 2004-03-03 Methodes et outils pour le criblage d’arn actifs in cellulo

Country Status (4)

Country Link
US (1) US20070122798A1 (fr)
EP (1) EP1599604A2 (fr)
FR (1) FR2852021B1 (fr)
WO (1) WO2004080378A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050164210A1 (en) * 2004-01-23 2005-07-28 Vivek Mittal Regulated polymerase III expression systems and related methods
DE102020211050B4 (de) 2020-09-02 2022-05-19 Volkswagen Aktiengesellschaft Antriebseinrichtung für ein Kraftfahrzeug
WO2022079083A1 (fr) * 2020-10-15 2022-04-21 F. Hoffmann-La Roche Ag Constructions d'acide nucléique pour transcription de va-arn

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2687411A1 (fr) * 1992-02-13 1993-08-20 Nice Sophia Antipolis Universi Vecteur comportant un gene viral transcrit par l'arn polymerase iii, et procede de production intracellulaire d'arn.
WO1995006718A2 (fr) * 1993-09-03 1995-03-09 Viagene, Inc. Procede de suppression de la reponse auto-immune
WO1997027309A1 (fr) * 1996-01-23 1997-07-31 Institut National De La Recherche Agronomique - I.N.R.A. Constructions d'adn et vecteurs d'expression derives du gene de l'arn va i d'adenovirus
WO1998032880A1 (fr) * 1997-01-23 1998-07-30 Immusol Incorporated Analyse fonctionnelle et decouverte de genes a l'aide de banques de vecteurs de genes de ribozymes specifiques a une cible ou bien rendues aleatoires
WO1999041371A1 (fr) * 1998-02-13 1999-08-19 Genetrace Systems, Inc. Utilisation de ribozymes afin d'identifier la fonction de genes
WO2003022311A1 (fr) * 2001-09-13 2003-03-20 Genvec, Inc. Vecteur adenoviral, systeme correspondant et procedes de fabrication et d'utilisation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837503A (en) * 1993-07-07 1998-11-17 Universite De Nice-Sophia-Antipolis Vector containing viral gene transcribed by RNA polymerase III and methods for use
US6183959B1 (en) * 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
WO2004009796A2 (fr) * 2002-07-24 2004-01-29 Immusol Incorporated Systeme a un seul promoteur pour la production de cassettes d'expression d'arnsi et genotheque d'expression reposant sur l'utilisation d'un lieur en epingle a cheveux d'amorce de polymerase

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2687411A1 (fr) * 1992-02-13 1993-08-20 Nice Sophia Antipolis Universi Vecteur comportant un gene viral transcrit par l'arn polymerase iii, et procede de production intracellulaire d'arn.
WO1995006718A2 (fr) * 1993-09-03 1995-03-09 Viagene, Inc. Procede de suppression de la reponse auto-immune
WO1997027309A1 (fr) * 1996-01-23 1997-07-31 Institut National De La Recherche Agronomique - I.N.R.A. Constructions d'adn et vecteurs d'expression derives du gene de l'arn va i d'adenovirus
WO1998032880A1 (fr) * 1997-01-23 1998-07-30 Immusol Incorporated Analyse fonctionnelle et decouverte de genes a l'aide de banques de vecteurs de genes de ribozymes specifiques a une cible ou bien rendues aleatoires
WO1999041371A1 (fr) * 1998-02-13 1999-08-19 Genetrace Systems, Inc. Utilisation de ribozymes afin d'identifier la fonction de genes
WO2003022311A1 (fr) * 2001-09-13 2003-03-20 Genvec, Inc. Vecteur adenoviral, systeme correspondant et procedes de fabrication et d'utilisation

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 17 May 1994 (1994-05-17), "Adenovirus 2 VA I gene with anti-Tat insert.", XP002309698, retrieved from EBI accession no. GSN:AAQ51311 Database accession no. AAQ51311 *
DATABASE Geneseq [online] 4 October 1995 (1995-10-04), "Adenovirus 2 (Ad2) VARNA1 promoter forward strand.", XP002309697, retrieved from EBI accession no. GSN:AAQ85743 Database accession no. AAQ85743 *
ELOIT M ET AL: "High level of transgene expression in cell cultures and in the mouse by replication-incompetent adenoviruses harboring modified VAI genes", JOURNAL OF VIROLOGY, vol. 71, no. 7, 1997, pages 5375 - 5381, XP002269684, ISSN: 0022-538X *
GWIZDEK CAROLE ET AL: "Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm: Terminal minihelix and RNA export", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 28, 13 July 2001 (2001-07-13), pages 25910 - 25918, XP002269631, ISSN: 0021-9258 *
ILVES HEINI ET AL: "Retroviral vectors designed for targeted expression of RNA polymerase III-driven transcripts: A comparative study", GENE (AMSTERDAM), vol. 171, no. 2, 1996, pages 203 - 208, XP002269685, ISSN: 0378-1119 *
MEDINA M F C ET AL: "RNA POLYMERASE III-DRIVEN EXPRESSION CASSETTES IN HUMAN GENE THERAPY", CURRENT OPINION IN MOLECULAR THERAPEUTICS, CURRENT DRUGS, LONDON,, GB, vol. 1, no. 5, October 1999 (1999-10-01), pages 580 - 594, XP001055319, ISSN: 1464-8431 *
YU M ET AL: "IN VITRO AND IN VIVO CHARACTERIZATION OF A SECOND FUNCTIONAL HAIRPIN RIBOZYME AGAINST HIV-1", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 206, no. 1, 1995, pages 381 - 386, XP000652043, ISSN: 0042-6822 *

Also Published As

Publication number Publication date
EP1599604A2 (fr) 2005-11-30
FR2852021B1 (fr) 2007-12-07
WO2004080378A2 (fr) 2004-09-23
US20070122798A1 (en) 2007-05-31
FR2852021A1 (fr) 2004-09-10

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