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WO2004063699A3 - Detection rapide de micro-organismes - Google Patents

Detection rapide de micro-organismes Download PDF

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Publication number
WO2004063699A3
WO2004063699A3 PCT/US2003/038399 US0338399W WO2004063699A3 WO 2004063699 A3 WO2004063699 A3 WO 2004063699A3 US 0338399 W US0338399 W US 0338399W WO 2004063699 A3 WO2004063699 A3 WO 2004063699A3
Authority
WO
WIPO (PCT)
Prior art keywords
primer
probe
microorganisms
yeast
mold
Prior art date
Application number
PCT/US2003/038399
Other languages
English (en)
Other versions
WO2004063699A2 (fr
Inventor
Hua Wang
Hongliang Luo
Chris Connor
Steven Schwartz
Ahmed Yousef
Wan Kai
Original Assignee
Univ Ohio State Res Found
Hua Wang
Hongliang Luo
Chris Connor
Steven Schwartz
Ahmed Yousef
Wan Kai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ohio State Res Found, Hua Wang, Hongliang Luo, Chris Connor, Steven Schwartz, Ahmed Yousef, Wan Kai filed Critical Univ Ohio State Res Found
Priority to AU2003302326A priority Critical patent/AU2003302326A1/en
Publication of WO2004063699A2 publication Critical patent/WO2004063699A2/fr
Publication of WO2004063699A3 publication Critical patent/WO2004063699A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des outils et des procédés permettant de détecter la présence de bactéries, de levure et de moisissure dans un échantillon obtenu à partir d'un échantillon alimentaire. Ces procédés mettent en oeuvre une réaction en chaîne de polymérase et des ensembles d'amorces et de sondes qui reposent sur l'ARNr 16S et les gènes de la squalène-hopène cyclase d'Alicyclobacillus et Geobacillus et le gène d'ADNr 18S de moisissure et de levure. Cette invention a aussi pour objet des ensembles d'amorces et de sondes. Chaque ensemble comprend une amorce et une amorce inverse, qui proviennent toutes deux des nucléotides compris entre 15 et 35 dans la longueur, ainsi qu'une sonde.
PCT/US2003/038399 2002-12-02 2003-12-02 Detection rapide de micro-organismes WO2004063699A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003302326A AU2003302326A1 (en) 2002-12-02 2003-12-02 Rapid detection of microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43020202P 2002-12-02 2002-12-02
US60/430,202 2002-12-02

Publications (2)

Publication Number Publication Date
WO2004063699A2 WO2004063699A2 (fr) 2004-07-29
WO2004063699A3 true WO2004063699A3 (fr) 2004-12-16

Family

ID=32712970

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/038399 WO2004063699A2 (fr) 2002-12-02 2003-12-02 Detection rapide de micro-organismes

Country Status (2)

Country Link
AU (1) AU2003302326A1 (fr)
WO (1) WO2004063699A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10344057B3 (de) * 2003-09-23 2005-06-09 Vermicon Ag Verfahren zum spezifischen Schnellnachweis getränkeschädlicher Mikroorganismen
FR3000105B1 (fr) * 2012-12-24 2015-05-15 Lvmh Rech Aptameres inhibiteurs de l'activite enzymatique de la tyrosinase
US9382591B2 (en) * 2013-06-06 2016-07-05 Pall Corporation Compositions for detecting Alicyclobacillus microorganisms
GB201507170D0 (en) 2015-04-24 2015-06-10 Givaudan Sa Process
GB201507207D0 (en) 2015-04-24 2015-06-10 Givaudan Sa Enzymes and applications thereof
GB201618090D0 (en) 2016-10-26 2016-12-07 Givaudan Sa Product

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002070728A2 (fr) * 2001-03-01 2002-09-12 The Johns Hopkins University Analyse quantitative pour detection et speciation simultanees d'infections bacteriennes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002070728A2 (fr) * 2001-03-01 2002-09-12 The Johns Hopkins University Analyse quantitative pour detection et speciation simultanees d'infections bacteriennes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YAMAZAKI K. ET AL: "Specific primers for detection of Alicyclobacillus acidoterrestris by RT-PCR", LETTERS IN APPLIED MICROBIOLOGY, vol. 23, November 1996 (1996-11-01), pages 350 - 354, XP002943407 *

Also Published As

Publication number Publication date
WO2004063699A2 (fr) 2004-07-29
AU2003302326A8 (en) 2004-08-10
AU2003302326A1 (en) 2004-08-10

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