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WO2004056964A3 - Vectors for inducible rna interference - Google Patents

Vectors for inducible rna interference Download PDF

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Publication number
WO2004056964A3
WO2004056964A3 PCT/US2003/040548 US0340548W WO2004056964A3 WO 2004056964 A3 WO2004056964 A3 WO 2004056964A3 US 0340548 W US0340548 W US 0340548W WO 2004056964 A3 WO2004056964 A3 WO 2004056964A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
rna interference
inducible rna
inducible
interfere
Prior art date
Application number
PCT/US2003/040548
Other languages
French (fr)
Other versions
WO2004056964A2 (en
Inventor
William Winston
Ronan C O'hagan
Joerg Heyer
William Rideout
Bijan Etemad-Moghadam
Original Assignee
Genpath Pharmaceuticals Inc
William Winston
Ronan C O'hagan
Joerg Heyer
William Rideout
Bijan Etemad-Moghadam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genpath Pharmaceuticals Inc, William Winston, Ronan C O'hagan, Joerg Heyer, William Rideout, Bijan Etemad-Moghadam filed Critical Genpath Pharmaceuticals Inc
Priority to AU2003299732A priority Critical patent/AU2003299732A1/en
Publication of WO2004056964A2 publication Critical patent/WO2004056964A2/en
Publication of WO2004056964A3 publication Critical patent/WO2004056964A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Recombinant vectors for inducibly expressing double-stranded RNA molecules that interfere with the expression of a target gene.
PCT/US2003/040548 2002-12-18 2003-12-18 Vectors for inducible rna interference WO2004056964A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003299732A AU2003299732A1 (en) 2002-12-18 2003-12-18 Vectors for inducible rna interference

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US43485602P 2002-12-18 2002-12-18
US60/434,856 2002-12-18
US49931303P 2003-08-28 2003-08-28
US60/499,313 2003-08-28

Publications (2)

Publication Number Publication Date
WO2004056964A2 WO2004056964A2 (en) 2004-07-08
WO2004056964A3 true WO2004056964A3 (en) 2004-10-14

Family

ID=32685340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/040548 WO2004056964A2 (en) 2002-12-18 2003-12-18 Vectors for inducible rna interference

Country Status (2)

Country Link
AU (1) AU2003299732A1 (en)
WO (1) WO2004056964A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8198077B2 (en) 2003-01-17 2012-06-12 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Inducible small RNA expression constructs for targeted gene silencing
FR2877674A1 (en) * 2004-11-08 2006-05-12 Genoxay Sa MODEL ANIMALS COMPRISING AT LEAST ONE TRANSGENE AND A SEQUENCE PERMITTING CONTROLLED EXPRESSION OF AN INTERFERING RNA WITH SAID TRANSGENE
AU2005318999B2 (en) 2004-12-22 2012-07-19 Genentech, Inc. Methods for producing soluble multi-membrane-spanning proteins
EP1888750A1 (en) * 2005-06-09 2008-02-20 ARTEMIS Pharmaceuticals GmbH Shrna and sirna expression in a living organism under control op a codon-optimized tetracycline repressor gene
EP1731607A1 (en) * 2005-06-09 2006-12-13 ARTEMIS Pharmaceuticals GmbH shRNA and siRNA expression in a living organism under control of a codon-optimized tetracycline repressor gene
WO2007014363A2 (en) * 2005-07-27 2007-02-01 Genentech, Inc. Vectors for inducible expression of hairpin rna and use thereof
WO2007035962A2 (en) * 2005-09-23 2007-03-29 California Institute Of Technology Gene blocking method
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
EP3219799A1 (en) * 2016-03-17 2017-09-20 IMBA-Institut für Molekulare Biotechnologie GmbH Conditional crispr sgrna expression
CN110540995B (en) * 2018-05-28 2023-01-10 南京农业大学 Myosin-5 protein reduction and application thereof in cyanoacrylate drug resistance treatment

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5917122A (en) * 1992-08-26 1999-06-29 Byrne; Guerard Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic mice
US20040002077A1 (en) * 2001-11-28 2004-01-01 Center For Advanced Science And Technology Incubation, Ltd. siRNA expression system and method for producing functional gene knock-down cell using the system
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040115815A1 (en) * 2002-07-24 2004-06-17 Immusol, Inc. Single promoter system for making siRNA expression cassettes and expression libraries using a polymerase primer hairpin linker

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5917122A (en) * 1992-08-26 1999-06-29 Byrne; Guerard Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic mice
US20040002077A1 (en) * 2001-11-28 2004-01-01 Center For Advanced Science And Technology Incubation, Ltd. siRNA expression system and method for producing functional gene knock-down cell using the system
US20040005593A1 (en) * 2002-03-06 2004-01-08 Rigel Pharmaceuticals, Inc. Novel method for delivery and intracellular synthesis of siRNA molecules
US20040115815A1 (en) * 2002-07-24 2004-06-17 Immusol, Inc. Single promoter system for making siRNA expression cassettes and expression libraries using a polymerase primer hairpin linker

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CZAUDERNA FRANK; ET AL: "Inducible shRNA expression for application in a prostate cancer mouse model", NUCLEIC ACIDS RESEARCH, vol. 31, no. 21, 1 November 2003 (2003-11-01), pages E127-1 - E127-7, XP002291076 *
ELBASHIR S.M. ET AL: "Analysis of gene function in somatic mammalian cells using small interfering RNAs", METHODS, vol. 26, no. 2, February 2002 (2002-02-01), pages 199 - 213, XP002251055 *
HANNON G.J.: "RNA interference", NATURE, vol. 418, 11 July 2002 (2002-07-11), pages 244 - 251, XP002979088 *
HASUWA H. ET AL: "Small interfering RNA and gene silencing in transgenic mice and rats", FEBS LETTERS, vol. 532, 13 November 2002 (2002-11-13), pages 227 - 230, XP004395375 *
OGUETA ET AL: "Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector", MOLECULAR MEDICINE, vol. 7, no. 8, August 2001 (2001-08-01), pages 569 - 579, XP002960124 *
OHKAWA ET AL: "Control of the functional activity of an antisense RNA by a tetracyclin-responsive derivative of the human U6 snRNA promoter", HUMAN GENE THERAPY, vol. 11, no. 4, 1 March 2000 (2000-03-01), pages 577 - 585, XP000926522 *
SASAKI ET AL: "A system for conditional RNA interference in the mouse using the lac operator - repressor system", SOCIETY FOR NEUROSCIENCE ABSTRACT VIEWER AND ITINERARY PLANNER, vol. 2003, no. ABSTRACT NO. 325.4, 8 November 2003 (2003-11-08), XP002979518 *
SUI ET AL: "A DNA vector-based RNAi technology to suppress gene expression in mammalian cells", PNAS, vol. 99, no. 8, 16 April 2002 (2002-04-16), pages 5515 - 5520, XP002964701 *

Also Published As

Publication number Publication date
WO2004056964A2 (en) 2004-07-08
AU2003299732A1 (en) 2004-07-14
AU2003299732A8 (en) 2004-07-14

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