+

WO2003035829A3 - Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules - Google Patents

Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules Download PDF

Info

Publication number
WO2003035829A3
WO2003035829A3 PCT/US2002/032088 US0232088W WO03035829A3 WO 2003035829 A3 WO2003035829 A3 WO 2003035829A3 US 0232088 W US0232088 W US 0232088W WO 03035829 A3 WO03035829 A3 WO 03035829A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
methods
nucleic acid
provides
oligonucleotides
Prior art date
Application number
PCT/US2002/032088
Other languages
English (en)
Other versions
WO2003035829A2 (fr
Inventor
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Original Assignee
Nanosphere Inc
So-Jung Park
Thomas A Taton
Chad A Mirkin
Robert L Letsinger
Robert C Mucic
James J Storhoff
Robert Elghanian
Viswanadham Garimella
Zhi Li
Gang Lu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/008,978 external-priority patent/US6984491B2/en
Application filed by Nanosphere Inc, So-Jung Park, Thomas A Taton, Chad A Mirkin, Robert L Letsinger, Robert C Mucic, James J Storhoff, Robert Elghanian, Viswanadham Garimella, Zhi Li, Gang Lu filed Critical Nanosphere Inc
Priority to JP2003538330A priority Critical patent/JP4347049B2/ja
Priority to EP02799155A priority patent/EP1478774A4/fr
Priority to AU2002363062A priority patent/AU2002363062B2/en
Priority to CA2463323A priority patent/CA2463323C/fr
Publication of WO2003035829A2 publication Critical patent/WO2003035829A2/fr
Publication of WO2003035829A3 publication Critical patent/WO2003035829A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés servant à détecter un acide nucléique. Ces procédés consistent à mettre en contact cet acide nucléique avec un ou plusieurs types de particules auxquelles sont fixés des oligonucléotides. Dans un mode de réalisation de ce procédé, les oligonucléotides sont liés à des nanoparticules et possèdent des séquences complémentaires de parties de la séquence de l'acide nucléique. L'hybridation des oligonucléotides sur les nanoparticules à l'acide nucléique provoque une modification pouvant être détectée (de préférence, une modification de couleur). Elle concerne également des compositions et des trousses contenant des particules. Elle concerne, de plus, des procédés de synthèse de conjugués uniques constitués par des nanoparticules et des oligonucléotides, les conjugués obtenus au moyen de ces procédés et des procédés de mise en application de ces conjugués. Elle concerne également des nanomatériaux et des nanostructures comprenant des nanoparticules, ainsi que des procédés de nanofabrication au moyen de ces nanoparticules. Elle concerne enfin un procédé de séparation d'un acide nucléique sélectionné d'autres acides nucléiques.
PCT/US2002/032088 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules WO2003035829A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003538330A JP4347049B2 (ja) 2001-10-09 2002-10-08 オリゴヌクレオチドを付着させたナノ粒子とその使用方法
EP02799155A EP1478774A4 (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules
AU2002363062A AU2002363062B2 (en) 2001-10-09 2002-10-08 Nanoparticles having oligonucleotides attached thereto and uses therefor
CA2463323A CA2463323C (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32786401P 2001-10-09 2001-10-09
US60/327,864 2001-10-09
US10/008,978 US6984491B2 (en) 1996-07-29 2001-12-07 Nanoparticles having oligonucleotides attached thereto and uses therefor
US10/008,978 2001-12-07

Publications (2)

Publication Number Publication Date
WO2003035829A2 WO2003035829A2 (fr) 2003-05-01
WO2003035829A3 true WO2003035829A3 (fr) 2004-08-26

Family

ID=32684383

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/032088 WO2003035829A2 (fr) 2001-10-09 2002-10-08 Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules

Country Status (5)

Country Link
EP (1) EP1478774A4 (fr)
JP (1) JP4347049B2 (fr)
AU (1) AU2002363062B2 (fr)
CA (1) CA2463323C (fr)
WO (1) WO2003035829A2 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7396677B2 (en) * 2003-11-07 2008-07-08 Nanosphere, Inc. Method of preparing nucleic acids for detection
JP4619012B2 (ja) * 2004-02-19 2011-01-26 トヨタ自動車株式会社 三次元ｄｎａネットワークの構築方法
ES2242528B1 (es) * 2004-03-25 2006-12-01 Consejo Sup. Investig. Cientificas Nanoparticulas magneticas de metales nobles.
SG152260A1 (en) * 2004-04-23 2009-05-29 Becton Dickinson Co Surface enhanced spectroscopy-active composite nanoparticles
WO2005116226A2 (fr) * 2004-05-24 2005-12-08 Midatech Ltd Nanoparticules comprenant des ligands d'arn
JP4822753B2 (ja) * 2005-07-11 2011-11-24 一般社団法人オンチップ・セロミクス・コンソーシアム 細胞構成物質分取チップならびに細胞構成物質解析システム及びこれらを用いる細胞構成物質解析法
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
EP1801593A1 (fr) * 2005-12-22 2007-06-27 Koninklijke Philips Electronics N.V. Une méthode d'imagérie d'échantillons biologiques au moyen des nanoparticules comme des agents de marquage
ES2493990T3 (es) * 2006-02-08 2014-09-12 Becton Dickinson And Company Ensayos con nanoetiquetas SERS
JP5391744B2 (ja) * 2009-03-09 2014-01-15 住友ベークライト株式会社 アレイ用基板
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP5200069B2 (ja) * 2010-07-27 2013-05-15 トヨタ自動車株式会社 三次元ｄｎａネットワーク
KR101792262B1 (ko) * 2011-10-10 2017-11-02 삼성전기주식회사 도전성 페이스트, 이의 제조방법 및 이를 이용한 적층 세라믹 전자부품
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
EP3480321B8 (fr) 2013-08-28 2021-03-10 Becton, Dickinson and Company Analyse de cellule unique massivement parallèle
EP3087202B1 (fr) * 2013-12-23 2020-10-14 The University Of Queensland Procédé et kit de détection d'acide nucléique
WO2015159535A1 (fr) * 2014-04-16 2015-10-22 富士フイルム株式会社 Solution de detection, son procede de preparation, et procede de biodetection
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
WO2016160844A2 (fr) 2015-03-30 2016-10-06 Cellular Research, Inc. Procédés et compositions pour codage à barres combinatoire
EP3286326B1 (fr) 2015-04-23 2025-01-22 Becton, Dickinson and Company Procédé pour l'amplification de transcriptome entier
AU2016270823B2 (en) 2015-06-01 2020-09-03 California Institute Of Technology Compositions and methods for screening T cells with antigens for specific populations
KR102395450B1 (ko) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. 핵산 라이브러리 정규화를 위한 방법 및 조성물
ES2928217T3 (es) * 2016-05-18 2022-11-16 Integrated Nano Tech Inc Método para la detección de un producto de PCR
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
BR112019005900A2 (pt) 2016-09-26 2019-06-11 Becton Dickinson Co medição de expressão de proteínas usando reagentes com sequências de oligonucleotídeos com código de barras
WO2018119128A1 (fr) * 2016-12-22 2018-06-28 Burris Robert Barton Procédés d'amplification non enzymatique d'un signal et leurs utilisations pour détecter et quantifier un analyte cible
EP3577232A1 (fr) 2017-02-01 2019-12-11 Cellular Research, Inc. Amplification sélective au moyen d'oligonucléotides de blocage
US12258613B2 (en) 2017-03-08 2025-03-25 California Institute Of Technology Pairing antigen specificity of a T cell with T cell receptor sequences
WO2018165566A1 (fr) 2017-03-09 2018-09-13 Auburn University Circuit différentiel pour correction du fond dans des mesures électrochimiques
EP4345172A3 (fr) 2017-06-05 2024-07-03 Becton, Dickinson and Company Indexation d'échantillon pour cellules individuelles
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
KR20190011117A (ko) * 2017-07-24 2019-02-01 사회복지법인 삼성생명공익재단 금 나노입자의 응집을 이용한 유전자의 변이를 검출하는 방법
EP4234717A3 (fr) 2018-05-03 2023-11-01 Becton, Dickinson and Company Analyse multi-omique d'échantillons à haut débit
WO2019213237A1 (fr) 2018-05-03 2019-11-07 Becton, Dickinson And Company Codage à barres moléculaire sur des extrémités de transcrit en regard
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
EP3820453B1 (fr) * 2018-07-10 2024-09-04 The Regents of The University of California Particules et films enrobés de biomolécules et leurs utilisations
EP3861134B1 (fr) 2018-10-01 2024-09-04 Becton, Dickinson and Company Détermination de séquences de transcripts 5
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (fr) 2018-12-13 2020-06-18 Cellular Research, Inc. Extension sélective dans une analyse de transcriptome complet de cellule unique
EP3914728B1 (fr) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucléotides associés à des anticorps
WO2020167920A1 (fr) 2019-02-14 2020-08-20 Cellular Research, Inc. Amplification de transcriptome entier et ciblé hybride
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4407041A3 (fr) 2019-11-08 2024-09-25 Becton Dickinson and Company Utilisation d'un amorçage aléatoire pour obtenir des informations v(d)j de pleine longueur pour le séquençage du répertoire immunitaire
EP4090763B1 (fr) 2020-01-13 2024-12-04 Becton Dickinson and Company Procédés et compositions pour la quantification de protéines et d'arn
EP4471155A3 (fr) 2020-01-29 2024-12-18 Becton, Dickinson and Company Puits à code-barres pour la cartographie spatiale de cellules individuelles par séquençage
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021231779A1 (fr) 2020-05-14 2021-11-18 Becton, Dickinson And Company Amorces pour profilage de répertoire immunitaire
WO2021247593A1 (fr) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucléotides et billes pour le test d'expression génique 5 prime
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4247967A1 (fr) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profilage de protéines hautement exprimées et faiblement exprimées
KR102768840B1 (ko) * 2021-02-23 2025-02-18 모던밸류 주식회사 열제어부가 통합된, turn-off 방식의 광신호 측정용 액적 로딩 카트리지
CN113088517B (zh) * 2021-03-15 2023-10-03 华南师范大学 一种纳米探针构建方法
CN115178746B (zh) * 2022-07-13 2024-03-01 宁波大学 用于检测化妆水中汞离子的金纳米粒子的制备方法与用途
CN115369153B (zh) * 2022-10-25 2023-03-10 中国医学科学院北京协和医院 一种Smad4基因检测生物传感器及制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1301625B1 (fr) * 2000-03-28 2010-11-03 Nanosphere, Inc. Nanoparticules auxquelles sont attaches des oligonucleotides et leur utilisation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US6214560B1 (en) * 1996-04-25 2001-04-10 Genicon Sciences Corporation Analyte assay using particulate labels

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1478774A4 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7259252B2 (en) 1996-07-29 2007-08-21 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US8323888B2 (en) 1996-07-29 2012-12-04 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6986989B2 (en) 1996-07-29 2006-01-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7250499B2 (en) 1996-07-29 2007-07-31 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6962786B2 (en) 1996-07-29 2005-11-08 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7208587B2 (en) 1996-07-29 2007-04-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6969761B2 (en) 1996-07-29 2005-11-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7985539B2 (en) 2002-05-07 2011-07-26 Northwestern University Nanoparticle probes with raman spectroscopic fingerprints for analyte detection
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US11845986B2 (en) 2016-05-25 2023-12-19 Becton, Dickinson And Company Normalization of nucleic acid libraries

Also Published As

Publication number Publication date
WO2003035829A2 (fr) 2003-05-01
EP1478774A2 (fr) 2004-11-24
CA2463323C (fr) 2010-12-21
CA2463323A1 (fr) 2003-05-01
AU2002363062B2 (en) 2007-03-22
JP2005525084A (ja) 2005-08-25
EP1478774A4 (fr) 2006-11-29
JP4347049B2 (ja) 2009-10-21

Similar Documents

Publication Publication Date Title
WO2003035829A3 (fr) Detection electrique d'adn basee sur un ensemble et mettant en application des sondes sous forme de nanoparticules
WO2001051665A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et utilisations de ces dernieres
WO2001073123A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et leur utilisation
WO2002046472A3 (fr) Nanoparticules auxquelles sont attaches des oligonucleotides et utilisations de ces dernieres
WO2002018643A3 (fr) Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees
CA2262018A1 (fr) Nanoparticules sur lesquelles sont attaches des oligonucleotides et utilisations desdites nanoparticules
EP0774518A3 (fr) Sondes d'acides nucléiques complémentaires aux acides nucléiques du virus du Papillome humain, procédés associés et trousse d'essais
WO2001092572A1 (fr) Ensemble et procede de determination du type de hla
CA2371672A1 (fr) Methode de dosage d'un acide nucleique a l'aide d'un acide nucleique temoin
ATE502117T1 (de) Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen
WO2004083386A3 (fr) Procedes d'elaboration de sondes privees de sequences repetitives, et utilisations
WO2004072238A3 (fr) Nucleotides marques par des phosphates terminaux, presentant de nouveaux lieurs
ATE466107T1 (de) Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
AU2002348563A1 (en) Nucleic acid enrichment
CA2172722A1 (fr) Procedes et compositions pour le sequencage efficace d'acide nucleique
WO1999049082A3 (fr) Nucleotides modifies et methodes utiles pour le sequençage d'acides nucleiques
WO2003089906A3 (fr) Nanoparticules fonctionnalisees et procede d'utilisation
WO2005050171A3 (fr) Procedes et compositions se rapportant a des reactifs centraux simples
WO2002016594A3 (fr) Procedes et reactifs de clonage moleculaire
WO2008135929A3 (fr) Procédé colorimétrique et coffret de détection de séquences d'acide nucléique spécifiques à l'aide de nanoparticules métalliques fonctionnalisées par oligonucléotides modifiés
WO2002061133A3 (fr) Detection d'acide nucleique avec des sondes structurees
WO2007002463A3 (fr) Procedes de criblage de genotype au moyen de particules magnetiques
WO2003027328A3 (fr) Procedes, kits et compositions utilises dans la suppression d'une sonde detectable se liant a des sequences de repetition reparties de maniere aleatoire de l'acide nucleique genomique
CA2319757A1 (fr) Procede de detection de sequences cibles d'acide nucleique impliquant la transcription in vitro a partir d'un promoteur d'arn
WO2002034951A3 (fr) Compositions et procedes de detection du virus de l'immunodeficience humaine 2 (vih-2)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002799155

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002363062

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2463323

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003538330

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2002799155

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 2002363062

Country of ref document: AU

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载