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WO2003033732A3 - Echtzeitdetektion von dna-amplifikationsprodukten - Google Patents

Echtzeitdetektion von dna-amplifikationsprodukten Download PDF

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Publication number
WO2003033732A3
WO2003033732A3 PCT/EP2002/010800 EP0210800W WO03033732A3 WO 2003033732 A3 WO2003033732 A3 WO 2003033732A3 EP 0210800 W EP0210800 W EP 0210800W WO 03033732 A3 WO03033732 A3 WO 03033732A3
Authority
WO
WIPO (PCT)
Prior art keywords
real
time detection
amplification products
dna amplification
relates
Prior art date
Application number
PCT/EP2002/010800
Other languages
English (en)
French (fr)
Other versions
WO2003033732A2 (de
Inventor
Christian Drosten
Original Assignee
Bernhard Nocht Inst Fuer Trope
Christian Drosten
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bernhard Nocht Inst Fuer Trope, Christian Drosten filed Critical Bernhard Nocht Inst Fuer Trope
Publication of WO2003033732A2 publication Critical patent/WO2003033732A2/de
Publication of WO2003033732A3 publication Critical patent/WO2003033732A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Echtzeitdetektion von Desoxyribonukleinsäure-Amplifikaten. Ferner betrifft die Anmeldung partiell doppelsträngige Oligonukleotide, die im Rahmen der Echtzeitdetektion Anwendung finden, sowie Kits zur Durchführung des Verfahrens.
PCT/EP2002/010800 2001-10-11 2002-09-26 Echtzeitdetektion von dna-amplifikationsprodukten WO2003033732A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10150121A DE10150121B4 (de) 2001-10-11 2001-10-11 Echtzeitdetektion von DNA-Amplifikationsprodukten
DE10150121.8 2001-10-11

Publications (2)

Publication Number Publication Date
WO2003033732A2 WO2003033732A2 (de) 2003-04-24
WO2003033732A3 true WO2003033732A3 (de) 2003-11-13

Family

ID=7702130

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/010800 WO2003033732A2 (de) 2001-10-11 2002-09-26 Echtzeitdetektion von dna-amplifikationsprodukten

Country Status (2)

Country Link
DE (1) DE10150121B4 (de)
WO (1) WO2003033732A2 (de)

Families Citing this family (32)

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US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
EP1578399A4 (de) 2002-12-06 2007-11-28 Isis Pharmaceuticals Inc Verfahren für die rasche identifikation von pathogenen bei tier und mensch
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US7312036B2 (en) * 2004-03-22 2007-12-25 Isis Pharmaceuticals, Inc. Compositions for use in identification of viral hemorrhagic fever viruses
EP1766659A4 (de) 2004-05-24 2009-09-30 Ibis Biosciences Inc Massenspektrometrie mit selektiver ionenfiltrierung durch digitalen schwellenvergleich
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
WO2006094238A2 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
AU2006272776B2 (en) 2005-07-21 2012-01-19 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
CA2663029C (en) 2006-09-14 2016-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2344893B1 (de) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Mikroplatten-handhabungssysteme und verfahren
EP2349549B1 (de) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Mischpatronen, mischstationen und verwandte ausrüstungen, und system
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
WO2011008971A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Lift and mount apparatus
EP2454000A4 (de) 2009-07-17 2016-08-10 Ibis Biosciences Inc Systeme zur identifizierung von biowirkstoffen
EP2488656B1 (de) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Mehrfache verschiebungsverstärkung

Citations (2)

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US5824557A (en) * 1996-04-02 1998-10-20 Panvera Corporation Method for detecting and quantitating nucleic acid impurities in biochemical preparations
EP0900805A2 (de) * 1990-07-27 1999-03-10 Chiron Corporation Einführung selektiv spaltbarer und/oder abasischer Stellen in Oligonukleotidketten und Reagenzien dafür

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Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
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Non-Patent Citations (5)

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Title
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DROSTEN CHRISTIAN ET AL: "Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 40, no. 7, July 2002 (2002-07-01), pages 2323 - 2330, XP002251382, ISSN: 0095-1137 *
FREEMAN WILLARD M ET AL: "Quantitative RT-PCR: Pitfalls and potential", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 26, no. 1, January 1999 (1999-01-01), pages 112 - 118,120-122,124-12, XP002143860, ISSN: 0736-6205 *
PEOC'H D ET AL: "Efficient chemical synthesis of oligonucleotides containing a true abasic site", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 32, no. 2, 1991, pages 207 - 210, XP002171022, ISSN: 0040-4039 *
WITTWER C T ET AL: "CONTINUOUS FLUORESCENCE MONITORING OF RAPID CYCLE DNA AMPLIFICATION", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 22, no. 1, 1997, pages 130 - 131,134-138, XP000683698, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
DE10150121B4 (de) 2005-12-01
DE10150121A1 (de) 2003-04-30
WO2003033732A2 (de) 2003-04-24

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