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WO2003033530A3 - Antigenes mycobacteriens exprimes a haute tension en oxygene - Google Patents

Antigenes mycobacteriens exprimes a haute tension en oxygene Download PDF

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Publication number
WO2003033530A3
WO2003033530A3 PCT/GB2002/004647 GB0204647W WO03033530A3 WO 2003033530 A3 WO2003033530 A3 WO 2003033530A3 GB 0204647 W GB0204647 W GB 0204647W WO 03033530 A3 WO03033530 A3 WO 03033530A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
induced
mycobacterial
oxygen tension
identifying
Prior art date
Application number
PCT/GB2002/004647
Other languages
English (en)
Other versions
WO2003033530A2 (fr
Inventor
Brian James
Joanna Bacon
Philip March
Original Assignee
Health Prot Agency
Brian James
Joanna Bacon
Philip March
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Prot Agency, Brian James, Joanna Bacon, Philip March filed Critical Health Prot Agency
Priority to AU2002337287A priority Critical patent/AU2002337287A1/en
Publication of WO2003033530A2 publication Critical patent/WO2003033530A2/fr
Publication of WO2003033530A3 publication Critical patent/WO2003033530A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne un procédé permettant d'identifier des gènes mycobactériens dont l'expression est induite ou régulée positivement dans des conditions de culture continue caractérisées par une tension en oxygène dissous d'au moins 30 % de saturation en air mesurée à 37 °C, comparée à une tension en oxygène dissous allant jusqu'à 10 % de saturation en air, mesurée à 37 °C. Le procédé selon l'invention permet facultativement d'identifier des gènes mycobactériens simultanément induits ou régulés positivement dans des conditions de culture continue limitant le fer. Lesdits gènes induits ou régulés positivement forment la base de vaccins d'acides nucléiques ou constituent des cibles permettant de préparer des mycobactéries atténuées destinées à des vaccins contre les infections mycobactériennes. De même, les peptides codés par lesdits gènes induits ou régulés positivement sont utilisés dans des vaccins. Dans un autre mode de réalisation, les gènes/peptides identifiés constituent un moyen permettant d'identifier la présence d'une infection mycobactérienne dans un échantillon clinique, au moyen d'une sonde d'acide nucléique ou d'une détection d'anticorps.
PCT/GB2002/004647 2001-10-12 2002-10-14 Antigenes mycobacteriens exprimes a haute tension en oxygene WO2003033530A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002337287A AU2002337287A1 (en) 2001-10-12 2002-10-14 Mycobacterial antigens expressed under high oxygen tension

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0124593.5A GB0124593D0 (en) 2001-10-12 2001-10-12 Mycobacterial antigens expressed under high oxygen tension
GB0124593.5 2001-10-12

Publications (2)

Publication Number Publication Date
WO2003033530A2 WO2003033530A2 (fr) 2003-04-24
WO2003033530A3 true WO2003033530A3 (fr) 2004-02-26

Family

ID=9923760

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/004647 WO2003033530A2 (fr) 2001-10-12 2002-10-14 Antigenes mycobacteriens exprimes a haute tension en oxygene

Country Status (3)

Country Link
AU (1) AU2002337287A1 (fr)
GB (1) GB0124593D0 (fr)
WO (1) WO2003033530A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ519395A (en) * 2002-06-07 2004-04-30 Agres Ltd Techniques for identifying genes that are important for virulence in Mycobacterial vaccines
US7608277B2 (en) * 2004-12-01 2009-10-27 Gene Therapy Systems, Inc. Tuberculosis nucleic acids, polypeptides and immunogenic compositions
EP1910410B1 (fr) * 2005-07-01 2011-10-26 Forsyth Dental Infirmary for Children Essais de detection d'antigenes de la tuberculose et vaccins
CA2638761A1 (fr) * 2006-01-31 2007-08-09 Proteome Systems Limited Procede de diagnostic et de traitement d'une infection a m. tuberculosis et reactifs correspondants
CA2649688A1 (fr) 2006-03-14 2007-09-20 Oregon Health & Science University Procedes pour detecter une infection par mycobacterium tuberculosis
EP2501397B1 (fr) * 2009-11-20 2017-10-04 Oregon Health and Science University Procédés de génération de réponse immunitaire à la tuberculose
CN103122364A (zh) * 2011-11-21 2013-05-29 华中农业大学 抗结核抑制剂筛选的靶标基因Rv2498c及应用
CN104419710B (zh) * 2013-09-04 2017-06-16 上海市肺科医院 一种结核分枝杆菌的耐药新基因及其应用
US10213766B2 (en) 2015-09-18 2019-02-26 Chevron Phillips Chemical Company Lp Methods of preparing a catalyst

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COLER ET AL: "Vaccination with the T cell antigen Mtb 8.4 protects against challenge with Mycobacterium tuberculosis", JOURNAL OF IMMUNOLOGY, vol. 166, May 2001 (2001-05-01), pages 6227 - 6235, XP002961490, ISSN: 0022-1767 *
DATABASE EMBL [online] 1 January 1998 (1998-01-01), "Acyl carrier protein", XP002250930, retrieved from EBI accession no. P71603 Database accession no. P71603 *
DATABASE EMBL [online] 1 June 1998 (1998-06-01), "Putative maturase homolog", XP002250931, retrieved from EBI accession no. O53616 Database accession no. O53616 *
IMBODEN ET AL: "Construction and characterization of a partial Myco- bacterium tuberculosis cDNA library of genes expressed at reduced oxygen tension", GENE, vol. 213, no. 1-2, 1998, pages 107 - 117, XP004125009, ISSN: 0378-1119 *
JAMES ET AL: "The physiology and pathogenicity of Mycobacterium tuberculosis grown under controlled conditions in a defined medium", JOURNAL OF APPLIED MICROBIOLOGY, vol. 88, no. 4, 2000, pages 669 - 677, XP000929200, ISSN: 1364-5072 *
PEREZ-GUZMAN: "Progressive age-related changes in pulmonary tuberculosis images and the effect of diabetes", AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, vol. 162, 2000, pages 1738 - 1740, XP002250929 *
WONG ET AL: "Identification of fur, aconitase, and other proteins expressed by Mycobacterium tuberculosis under conditions of low and high concentrations of iron by combined two-dimensional gel electrophoresis and mass spectrometry", INFECTION AND IMMUNITY, vol. 67, no. 1, 1999, pages 327 - 336, XP002250955 *

Also Published As

Publication number Publication date
WO2003033530A2 (fr) 2003-04-24
GB0124593D0 (en) 2001-12-05
AU2002337287A1 (en) 2003-04-28

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