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WO2003016483A3 - Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides - Google Patents

Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides Download PDF

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Publication number
WO2003016483A3
WO2003016483A3 PCT/US2002/026063 US0226063W WO03016483A3 WO 2003016483 A3 WO2003016483 A3 WO 2003016483A3 US 0226063 W US0226063 W US 0226063W WO 03016483 A3 WO03016483 A3 WO 03016483A3
Authority
WO
WIPO (PCT)
Prior art keywords
crnas
probing
labelling
oligo
based microarrays
Prior art date
Application number
PCT/US2002/026063
Other languages
English (en)
Other versions
WO2003016483A2 (fr
Inventor
Tord Hjalt
Original Assignee
Curagen Corp
Tord Hjalt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curagen Corp, Tord Hjalt filed Critical Curagen Corp
Priority to JP2003521792A priority Critical patent/JP2005502340A/ja
Priority to CA002450215A priority patent/CA2450215A1/fr
Priority to EP02757157A priority patent/EP1451346A2/fr
Publication of WO2003016483A2 publication Critical patent/WO2003016483A2/fr
Publication of WO2003016483A3 publication Critical patent/WO2003016483A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Nouveau procédé de marquage d'ARNc utile pour sonder des microréseaux d'oligodésoxyribonucléotides. On soumet le modèle de polynucléotide voulu à une transcription inverse pour obtenir de l'ADNc bicaténaire en utilisant des amorces aléatoires incluant un promoteur d'ARN polymérase. Cet ADNc sert ensuite de modèle pour la synthèse d'ARN marqué par transcription in vitro en présence de précurseurs marqués.
PCT/US2002/026063 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides WO2003016483A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2003521792A JP2005502340A (ja) 2001-08-16 2002-08-16 オリゴベースマイクロアレイを探索するためのｃＲＮＡの標識方法
CA002450215A CA2450215A1 (fr) 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides
EP02757157A EP1451346A2 (fr) 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31289301P 2001-08-16 2001-08-16
US60/312,893 2001-08-16

Publications (2)

Publication Number Publication Date
WO2003016483A2 WO2003016483A2 (fr) 2003-02-27
WO2003016483A3 true WO2003016483A3 (fr) 2004-06-10

Family

ID=23213477

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/026063 WO2003016483A2 (fr) 2001-08-16 2002-08-16 Procede de marquage d'arnc pour sonder des microreseaux d'oligodesoxyribonucleotides

Country Status (4)

Country Link
EP (1) EP1451346A2 (fr)
JP (1) JP2005502340A (fr)
CA (1) CA2450215A1 (fr)
WO (1) WO2003016483A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017206A1 (fr) * 2003-08-13 2005-02-24 Affymetrix, Inc. Procedes et trousses pour la preparation d'echantillons d'acides nucleiques
JPWO2006041159A1 (ja) * 2004-10-14 2008-08-07 学校法人日本大学 プロモーター領域を付加したプライマー
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
WO2009137912A1 (fr) * 2008-05-15 2009-11-19 Topigen Pharmaceuticals Inc. Oligonucléotides pour le traitement de l’inflammation et de la prolifération des cellules néoplasiques

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA

Also Published As

Publication number Publication date
WO2003016483A2 (fr) 2003-02-27
EP1451346A2 (fr) 2004-09-01
CA2450215A1 (fr) 2003-02-27
JP2005502340A (ja) 2005-01-27

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