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WO2003015692A3 - Domaine de fixation aux ligands du recepteur du glucocorticoide cristallise et procedes de criblage l'utilisant - Google Patents

Domaine de fixation aux ligands du recepteur du glucocorticoide cristallise et procedes de criblage l'utilisant Download PDF

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Publication number
WO2003015692A3
WO2003015692A3 PCT/US2002/022648 US0222648W WO03015692A3 WO 2003015692 A3 WO2003015692 A3 WO 2003015692A3 US 0222648 W US0222648 W US 0222648W WO 03015692 A3 WO03015692 A3 WO 03015692A3
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WIPO (PCT)
Prior art keywords
test
polypeptide
crystallized
binding domain
ligand binding
Prior art date
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PCT/US2002/022648
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English (en)
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WO2003015692A2 (fr
Inventor
Christopher J Apolito
Randy K Bledsoe
Millard H Lambert Iii
David D Mckee
Valerie Gail Montana
Kenneth H Pearce
Thomas B Stanley
Huaqiang Eric Xu
Christopher J Delves
Original Assignee
Smithkline Beecham Corp
Christopher J Apolito
Randy K Bledsoe
Millard H Lambert Iii
David D Mckee
Valerie Gail Montana
Kenneth H Pearce
Thomas B Stanley
Huaqiang Eric Xu
Christopher J Delves
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smithkline Beecham Corp, Christopher J Apolito, Randy K Bledsoe, Millard H Lambert Iii, David D Mckee, Valerie Gail Montana, Kenneth H Pearce, Thomas B Stanley, Huaqiang Eric Xu, Christopher J Delves filed Critical Smithkline Beecham Corp
Priority to US10/484,061 priority Critical patent/US7238778B2/en
Priority to AU2002355929A priority patent/AU2002355929A1/en
Publication of WO2003015692A2 publication Critical patent/WO2003015692A2/fr
Publication of WO2003015692A3 publication Critical patent/WO2003015692A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/743—Steroid hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/30—Drug targeting using structural data; Docking or binding prediction
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Theoretical Computer Science (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
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Abstract

L'invention porte sur un procédé de modification d'un récepteur nucléaire d'essais (RNE) pouvant consister: à sélectionner une séquence de RNE présentant une caractéristique ciblée en vue de sa modification; à aligner ladite séquence de RNE avec au moins une séquence de récepteur nucléaire de référence (RNR) pour laquelle une structure obtenue par rayons X est disponible, et présentant une caractéristique souhaitée du RNE; à construire un modèle en 3D du RNE à l'aide des coordonnées en 3D de la structure obtenue par rayons X du RNR, et de l'alignement de ses séquences avec celle du RNE; à examiner le modèle en 3D du RNE pour y déceler les différences avec le RNR associés à la caractéristique souhaitée; à muter l'un au moins un résidu d'acides nucléiques de la séquence du RNE située au niveau d'une différence identifiée au-dessus d'un résidu associé à la caractéristique souhaitée, de manière à créer une modification du RNE. L'invention porte également: sur un récepteur de glucocorticoïdes (RG) isolé présentant une mutation d'un domaine de fixation de ligands modifiant la solubilité dudit domaine; sur un RG isolé ou ses parties fonctionnelles présentant une ou plusieurs mutations consistant à substituer un résidu d'acide nucléique hydrophobe à un résidu d'acide nucléique hydrophile, les mutations représentatives étant les substitutions F602S et F602D; sur l'expression du RG dans le E.coli; sur la structure cristalline en 3D résolue du domaine de fixation aux ligands du RGα ainsi que sur la forme cristalline dudit domaine; et sur des procédés d'élaboration de modulateurs de l'activité biologique du RGα et d'autres récepteurs nucléaires, récepteurs stéroïdiens, et récepteurs de glucocorticoïdes, et de leur domaine de fixation aux ligands.
PCT/US2002/022648 2001-07-17 2002-07-17 Domaine de fixation aux ligands du recepteur du glucocorticoide cristallise et procedes de criblage l'utilisant WO2003015692A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/484,061 US7238778B2 (en) 2001-07-17 2002-07-17 Crystallized glucocorticoid receptor ligand binding domain polypeptide and screening methods employing same
AU2002355929A AU2002355929A1 (en) 2001-07-17 2002-07-17 Crystallized glucocorticoid receptor ligand binding domain polypeptide and screening methods employing same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30590201P 2001-07-17 2001-07-17
US60/305,902 2001-07-17

Publications (2)

Publication Number Publication Date
WO2003015692A2 WO2003015692A2 (fr) 2003-02-27
WO2003015692A3 true WO2003015692A3 (fr) 2003-10-02

Family

ID=23182861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/022648 WO2003015692A2 (fr) 2001-07-17 2002-07-17 Domaine de fixation aux ligands du recepteur du glucocorticoide cristallise et procedes de criblage l'utilisant

Country Status (3)

Country Link
US (1) US7238778B2 (fr)
AU (1) AU2002355929A1 (fr)
WO (1) WO2003015692A2 (fr)

Families Citing this family (28)

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US6434489B1 (en) * 2000-04-03 2002-08-13 Schering Corporation Compositions of hepatitis C virus NS5B polymerase and methods for crystallizing same
GB0209507D0 (en) * 2002-04-25 2002-06-05 Karobio Ab Nuclear receptor structure
EP1375517A1 (fr) * 2002-06-21 2004-01-02 Smithkline Beecham Corporation Structure du domaine de liaison du ligand du récepteur de glucocorticoides comportant une poche de liaison expansée et procédé d'emploi
US7442554B2 (en) * 2002-07-18 2008-10-28 Bristol-Myers Squibb Company Compositions and methods involving glucocorticoid receptor site II
JP2006265173A (ja) * 2005-03-24 2006-10-05 Sumitomo Chemical Co Ltd グルココルチコイド受容体リガンド結合ドメインとリガンドとの複合体を含む結晶
WO2006117592A1 (fr) * 2005-04-29 2006-11-09 Inserm Procedes de modulation de recepteurs nucleaires
JP2007008897A (ja) * 2005-07-04 2007-01-18 Sumitomo Chemical Co Ltd グルココルチコイド受容体リガンド結合ドメインとリガンドとの複合体を含む結晶
US7910565B2 (en) 2006-09-01 2011-03-22 Wisconsin Alumni Research Foundation Prostate cancer vaccine
WO2010101565A1 (fr) * 2009-03-04 2010-09-10 Shriners Hospitals For Children Allèles de récepteurs des glucocorticoïdes et leurs utilisations
US20120095055A1 (en) * 2009-06-02 2012-04-19 Merck Sharp & Dohme, Corp. HEXAHYDROCYCLOPENTYL[f]INDAZOLE 5-HYDROXYMETHYL ETHANOLS AND DERIVATIVES THEREOF AS SELECTIVE GLUCOCORTICOID RECEPTOR MODULATORS
WO2011031574A1 (fr) * 2009-09-08 2011-03-17 Merck Sharp & Dohme Corp. Ethanols d'hexahydrocyclopenta[f]indazole 5-yle et leurs dérivés utilisés comme modulateurs sélectifs des récepteurs des glucocorticoïdes
AU2011293193A1 (en) 2010-08-27 2013-02-14 Corcept Therapeutics, Inc. Pyridyl-amine fused azadecalin modulators
US9189594B2 (en) 2010-08-31 2015-11-17 Annai Systems Inc. Method and systems for processing polymeric sequence data and related information
WO2012094618A1 (fr) 2011-01-07 2012-07-12 Corcept Therapeutics, Inc. Traitement combiné associant un stéroïde et un antagoniste des récepteurs des glucocorticoïdes
US8859774B2 (en) 2012-05-25 2014-10-14 Corcept Therapeutics, Inc. Heteroaryl-ketone fused azadecalin glucocorticoid receptor modulators
US12226412B2 (en) 2012-05-25 2025-02-18 Corcept Therapeutics, Inc. Heteroaryl-ketone fused azadecalin glucocorticoid receptor modulators
AU2013277986B2 (en) 2012-06-22 2016-12-01 Annai Systems Inc. System and method for secure, high-speed transfer of very large files
RU2674983C1 (ru) 2013-11-25 2018-12-14 Корсепт Терапеутикс, Инк. Октагидро конденсированные азадекалиновые модуляторы глюкокортикоидного рецептора
RU2019129299A (ru) 2017-03-31 2021-04-30 Корсепт Терапьютикс, Инк. Модуляторы глюкокортикоидного рецептора для лечения рака шейки матки
US11234971B2 (en) 2018-12-19 2022-02-01 Corcept Therapeutics Incorporated Methods of treating cancer comprising administration of a glucocorticoid receptor modulator and a cancer chemotherapy agent
US11389432B2 (en) 2018-12-19 2022-07-19 Corcept Therapeutics Incorporated Methods of treating cancer comprising administration of a glucocorticoid receptor modulator and a cancer chemotherapy agent
CN113194931B (zh) 2018-12-19 2023-11-10 科塞普特治疗公司 含有瑞拉可兰、杂芳基-酮稠合的氮杂十氢化萘化合物的药物制剂
US11058670B2 (en) 2019-02-22 2021-07-13 Corcept Therapeutics Incorporated Therapeutic uses of relacorilant, a heteroaryl-ketone fused azadecalin glucocorticoid receptor modulator
PH12022551430A1 (en) 2019-12-11 2023-11-20 Corcept Therapeutics Inc Methods of treating antipsychotic-induced weight gain with miricorilant
CN112695052A (zh) * 2020-12-25 2021-04-23 华南农业大学 一种重组人糖皮质激素受体GRα-His蛋白及其表达和纯化方法
CN112553231A (zh) * 2020-12-25 2021-03-26 华南农业大学 一种重组人热休克蛋白HSP90-His及其表达和纯化方法
WO2022134033A1 (fr) 2020-12-25 2022-06-30 Corcept Therapeutics Incorporated Procédés de préparation de modulateurs du récepteur des glucocorticoïdes d'azadécaline fusionnée hétéroaryle-cétone
CN119790079A (zh) * 2022-06-27 2025-04-08 上海奕拓医药科技有限责任公司 用于调节分子的组合物和方法

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Non-Patent Citations (3)

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Publication number Publication date
WO2003015692A2 (fr) 2003-02-27
US7238778B2 (en) 2007-07-03
AU2002355929A1 (en) 2003-03-03
US20050181362A1 (en) 2005-08-18

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