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WO2003012090A3 - Thermal tolerant avicelase from acidothermus cellulolyticus - Google Patents

Thermal tolerant avicelase from acidothermus cellulolyticus Download PDF

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Publication number
WO2003012090A3
WO2003012090A3 PCT/US2001/023818 US0123818W WO03012090A3 WO 2003012090 A3 WO2003012090 A3 WO 2003012090A3 US 0123818 W US0123818 W US 0123818W WO 03012090 A3 WO03012090 A3 WO 03012090A3
Authority
WO
WIPO (PCT)
Prior art keywords
avicelase
acidothermus cellulolyticus
aviiii
thermal tolerant
cellulase
Prior art date
Application number
PCT/US2001/023818
Other languages
French (fr)
Other versions
WO2003012090A2 (en
Inventor
Shi-You Ding
William S Adney
Todd B Vinzant
Michael E Himmel
Original Assignee
Midwest Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Midwest Research Inst filed Critical Midwest Research Inst
Priority to AU2001277220A priority Critical patent/AU2001277220A1/en
Priority to PCT/US2001/023818 priority patent/WO2003012090A2/en
Publication of WO2003012090A2 publication Critical patent/WO2003012090A2/en
Publication of WO2003012090A3 publication Critical patent/WO2003012090A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a thermal tolerant (thermostable) cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as AviIII. AviIII has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified AviIII. Methods of making and using AvIII polypeptides, including fusions, variants, and derivatives, are also disclosed.
PCT/US2001/023818 2001-07-28 2001-07-28 Thermal tolerant avicelase from acidothermus cellulolyticus WO2003012090A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001277220A AU2001277220A1 (en) 2001-07-28 2001-07-28 Thermal tolerant avicelase from acidothermus cellulolyticus
PCT/US2001/023818 WO2003012090A2 (en) 2001-07-28 2001-07-28 Thermal tolerant avicelase from acidothermus cellulolyticus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/023818 WO2003012090A2 (en) 2001-07-28 2001-07-28 Thermal tolerant avicelase from acidothermus cellulolyticus

Publications (2)

Publication Number Publication Date
WO2003012090A2 WO2003012090A2 (en) 2003-02-13
WO2003012090A3 true WO2003012090A3 (en) 2003-03-27

Family

ID=21742736

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/023818 WO2003012090A2 (en) 2001-07-28 2001-07-28 Thermal tolerant avicelase from acidothermus cellulolyticus

Country Status (2)

Country Link
AU (1) AU2001277220A1 (en)
WO (1) WO2003012090A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2699406C (en) 2007-09-12 2019-09-03 Martek Biosciences Corporation Biological oils and production and uses thereof
CN110835606B (en) 2014-07-03 2020-12-22 可持续生物制品公司 Fusarium oxysporum strain and methods of making and using same
DK3901246T3 (en) 2016-03-01 2024-09-23 The Fynder Group Inc Filamentous fungal biomats, methods of making and methods of using the same
JP2020532297A (en) 2017-08-30 2020-11-12 ザ・フィンダー・グループ・インコーポレイテッドＴｈｅ Ｆｙｎｄｅｒ Ｇｒｏｕｐ， Ｉｎｃ． Edible compositions containing filamentous fungi and bioreactor systems for their cultivation
EP4292436A3 (en) 2019-02-27 2024-03-20 The Fynder Group, Inc. Food materials comprising filamentous fungal particles and membrane bioreactor design
US20220325232A1 (en) 2019-06-18 2022-10-13 The Fynder Group, Inc. Fungal textile materials and leather analogs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536655A (en) * 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536655A (en) * 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1 November 1998 (1998-11-01), M. ARAI ET AL: "Avicelase III from Aspergillus aculeatus", XP002195538 *
DATABASE EMBL 11 February 2001 (2001-02-11), M.D. GIBBS ET AL: "Glycosyl hydrolase 5 (caldicellulosiruptor sp. Tok7B.1", XP002195537 *
DATABASE SWISS-PROT 1 August 1988 (1988-08-01), L.M. ROBSON ET AL: "Endoglucanase precursor (EC 3.2.1.4) (ENDO-1,4-beta -Glucanase) (Cellulase)", XP002195539 *
M.D. GIBBS ET AL: "Multidomain and multifunctional Glycosyl hydrolases from the extreme Thermophile Caldicellulosiruptor isolate Tok7B.1", CURRENT MICROBIOLOGY, vol. 40, no. 5, 2000, pages 333 - 340, XP002195536 *
ROBSON L M ET AL: "ENDO-BETA-1,4-GLUCANASE GENE OF BACILLUS SUBTILIS DLG", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 169, no. 5, 1 May 1987 (1987-05-01), pages 2017 - 2025, XP002046715, ISSN: 0021-9193 *
TOMME P ET AL: "Characterization and affinity applications of cellulose-binding domains", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 715, no. 1, 11 September 1998 (1998-09-11), pages 283 - 296, XP004147002, ISSN: 1570-0232 *

Also Published As

Publication number Publication date
AU2001277220A1 (en) 2003-02-17
WO2003012090A2 (en) 2003-02-13

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