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WO2003010507A3 - System for intracellular process monitoring and in vivo drug screening - Google Patents

System for intracellular process monitoring and in vivo drug screening Download PDF

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Publication number
WO2003010507A3
WO2003010507A3 PCT/IB2002/004257 IB0204257W WO03010507A3 WO 2003010507 A3 WO2003010507 A3 WO 2003010507A3 IB 0204257 W IB0204257 W IB 0204257W WO 03010507 A3 WO03010507 A3 WO 03010507A3
Authority
WO
WIPO (PCT)
Prior art keywords
drug screening
process monitoring
vivo drug
intracellular process
intracellular
Prior art date
Application number
PCT/IB2002/004257
Other languages
French (fr)
Other versions
WO2003010507A2 (en
Inventor
Inhwan Hwang
Dae Heon Kim
Yong Jik Lee
Jing Bo Jin
Original Assignee
Ahram Biosystems Inc
Inhwan Hwang
Dae Heon Kim
Yong Jik Lee
Jing Bo Jin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ahram Biosystems Inc, Inhwan Hwang, Dae Heon Kim, Yong Jik Lee, Jing Bo Jin filed Critical Ahram Biosystems Inc
Priority to AU2002339228A priority Critical patent/AU2002339228A1/en
Publication of WO2003010507A2 publication Critical patent/WO2003010507A2/en
Publication of WO2003010507A3 publication Critical patent/WO2003010507A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plant Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to a method for detecting characteristics related to intracellular trafficking and subcellular localization of a selected reporter protein. The invention has a wide spectrum of important applications including use in screens to detect compounds that modulate protein trafficking in vivo.
PCT/IB2002/004257 2001-07-16 2002-07-16 System for intracellular process monitoring and in vivo drug screening WO2003010507A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002339228A AU2002339228A1 (en) 2001-07-16 2002-07-16 System for intracellular process monitoring and in vivo drug screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2001-0042886 2001-07-16
KR1020010042886 2001-07-16

Publications (2)

Publication Number Publication Date
WO2003010507A2 WO2003010507A2 (en) 2003-02-06
WO2003010507A3 true WO2003010507A3 (en) 2004-05-27

Family

ID=19712212

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/KR2002/001345 WO2003008536A2 (en) 2001-07-16 2002-07-16 A system for intracellular process monitoring and in vivo drug screening
PCT/IB2002/004257 WO2003010507A2 (en) 2001-07-16 2002-07-16 System for intracellular process monitoring and in vivo drug screening

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/KR2002/001345 WO2003008536A2 (en) 2001-07-16 2002-07-16 A system for intracellular process monitoring and in vivo drug screening

Country Status (3)

Country Link
KR (2) KR20030007221A (en)
AU (1) AU2002339228A1 (en)
WO (2) WO2003008536A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030087869A1 (en) * 2001-09-28 2003-05-08 Ebens Allen James Assay for compounds affecting invertebrate cell secretory pathways
KR100863283B1 (en) * 2002-10-11 2008-10-15 아람 바이오시스템 주식회사 Observation method of intracellular process and recombinant genes used therefor
CA2580884A1 (en) * 2004-10-15 2006-04-20 Dsm Ip Assets B.V. Method for production of a compound in a eukaryotic cell
FR2920878B1 (en) * 2007-09-10 2019-07-26 Innovative Concepts In Drug Development (Icdd) METHOD FOR PREDICTIVE TOXICOLOGY OR EFFICACY TESTING BY MEASURING ORGANITY MOBILITY
CN108676075B (en) * 2018-06-01 2022-04-01 山西省农业科学院果树研究所 Method for guiding protein molecules into plant cell nucleus
CN109136321A (en) * 2018-08-29 2019-01-04 北京艾普希隆生物科技有限公司 A kind of plant sub-cellular location reagent box and its application
KR102288367B1 (en) * 2018-11-15 2021-08-11 주식회사 바이오앱 Recombinant vector expressing virus-like particles in plants and methods of preparing vaccine compositions comprising circovirus-like particles using the same
CN110734480B (en) * 2019-11-07 2021-08-24 中国科学院遗传与发育生物学研究所 Application of Escherichia coli Molecular Chaperone GroEL/ES in Assisting the Synthesis of Plant Rubisco
CN114317593A (en) * 2021-12-31 2022-04-12 北京林业大学 Method for observing plant protoplast membrane protein dynamics by using super-resolution microscopy
CN117230109B (en) * 2023-11-16 2024-03-19 三亚中国农业科学院国家南繁研究院 Visualized plasmid replacing fluorescent protein and related application thereof
CN119101709A (en) * 2024-09-10 2024-12-10 深圳市第三人民医院(深圳市肝病研究所) Dual fluorescence labeled living cell sample of human TREM2 protein and preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999003997A1 (en) * 1997-07-16 1999-01-28 University Of Florida Humanized and green fluorescent protein genes and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999003997A1 (en) * 1997-07-16 1999-01-28 University Of Florida Humanized and green fluorescent protein genes and methods

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BORDONNE ET AL.: "Functional characterization of nuclear localization signals in yeast Sm proteins", MOL. CELL. BIOL., vol. 21, 2000, pages 7943 - 7954 *
DATABASE BIOSIS [online] FEI ET AL.: "Nuclear trafficking of photoreceptor protein Crx: The targeting sequence and pathologic implications", Database accession no. 2000:429862 *
IOVS, vol. 41, no. 10, 2000, pages 2849 - 2856 *
KIM D. H.: "Trafficking of phosphatidylinositol 3-phosphate from the trans-golgi network to the lumen of the central vacuole in plant cells", THE PLANT CELL, vol. 13, February 2001 (2001-02-01), pages 287 - 301 *

Also Published As

Publication number Publication date
KR20030007221A (en) 2003-01-23
KR100919637B1 (en) 2009-09-30
AU2002339228A1 (en) 2003-02-17
WO2003010507A2 (en) 2003-02-06
KR20040015804A (en) 2004-02-19
WO2003008536A2 (en) 2003-01-30

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