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WO2003010292A3 - Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors - Google Patents

Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors Download PDF

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Publication number
WO2003010292A3
WO2003010292A3 PCT/US2002/023865 US0223865W WO03010292A3 WO 2003010292 A3 WO2003010292 A3 WO 2003010292A3 US 0223865 W US0223865 W US 0223865W WO 03010292 A3 WO03010292 A3 WO 03010292A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cell precursors
dendritic cell
enrichment
culture
Prior art date
Application number
PCT/US2002/023865
Other languages
French (fr)
Other versions
WO2003010292A2 (en
Inventor
Marnix L Bosch
Benjamin A Tjoa
Paul C Harris
Original Assignee
Northwest Biotherapeutics Inc
Marnix L Bosch
Benjamin A Tjoa
Paul C Harris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwest Biotherapeutics Inc, Marnix L Bosch, Benjamin A Tjoa, Paul C Harris filed Critical Northwest Biotherapeutics Inc
Priority to AU2002326463A priority Critical patent/AU2002326463A1/en
Publication of WO2003010292A2 publication Critical patent/WO2003010292A2/en
Publication of WO2003010292A3 publication Critical patent/WO2003010292A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/19—Dendritic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/24—Antigen-presenting cells [APC]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4274—Prostate associated antigens e.g. Prostate stem cell antigen [PSCA]; Prostate carcinoma tumor antigen [PCTA]; Prostatic acid phosphatase [PAP]; Prostate-specific G-protein-coupled receptor [PSGR]
    • A61K40/4276—Prostate specific membrane antigen [PSMA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0639—Dendritic cells, e.g. Langherhans cells in the epidermis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods and materials for isolating, culturing and differentiating monocytic dendritic cell precursors to form immature and/or mature dendritic cells. In a related aspect, methods, materials, and a system are provided for isolating, culturing and differentiating monocytic dendritic cell precursors under aseptic conditions to obtain immature and/or mature dendritic cells.
PCT/US2002/023865 2001-07-25 2002-07-25 Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors WO2003010292A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002326463A AU2002326463A1 (en) 2001-07-25 2002-07-25 Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30797801P 2001-07-25 2001-07-25
US60/307,978 2001-07-25

Publications (2)

Publication Number Publication Date
WO2003010292A2 WO2003010292A2 (en) 2003-02-06
WO2003010292A3 true WO2003010292A3 (en) 2003-05-22

Family

ID=23191999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/023865 WO2003010292A2 (en) 2001-07-25 2002-07-25 Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors

Country Status (2)

Country Link
AU (1) AU2002326463A1 (en)
WO (1) WO2003010292A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2459713C (en) * 2001-09-06 2015-08-18 Northwest Biotherapeutics, Inc. Compositions and methods for priming monocytic dendritic cells and t cells for th-1 response
DK2298436T3 (en) 2002-06-19 2014-11-10 Northwest Biotherapeutics Inc Using a tangential flow filtration device and methods for leukocyte enrichment
RU2348418C2 (en) 2002-12-06 2009-03-10 Норсуэст Байотерапьютикс, Инк. Introduction of dendritic cells exposed to partial aging in vitro, for tumour treatment
AU2004214896A1 (en) 2003-02-27 2004-09-10 Northwest Biotherapeutics, Inc. Generation of dendritic cells from monocytic dendritic precursor cells with GM-CSF in the absence of additional cytokines
RU2008122550A (en) 2005-12-08 2010-01-20 Норсуэст Байотерапьютикс, Инк. (Us) COMPOSITIONS AND METHODS FOR INDUCING ACTIVATION OF IMMATURE MONOCITARY DENDRITIC CELLS
CA2895280C (en) 2012-12-18 2021-04-06 Immunicum Ab Co-differentiation of monocytes from allogeneic donors
WO2017004169A1 (en) * 2015-06-29 2017-01-05 Northeastern University Dendritic cell generator
AU2016286112B2 (en) 2015-06-30 2023-02-16 Northwest Biotherapeutics, Inc. Optimally activated dendritic cells that induce an improved or increased anti-tumor immune response
EP3350318B1 (en) 2015-09-15 2022-04-13 NorthWest Biotherapeutics, Inc. Methods relating to activated dendritic cell compositions for subjects with advanced cancers
WO2017079674A1 (en) * 2015-11-04 2017-05-11 Northeastern University Systems for producing cellular immunotherapeutics and methods of use thereof
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
US11566217B2 (en) 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
CA3158509A1 (en) 2019-10-21 2021-04-29 Flaskworks, Llc Systems and methods for cell culturing

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994126A (en) * 1992-04-01 1999-11-30 The Rockefeller University Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994126A (en) * 1992-04-01 1999-11-30 The Rockefeller University Method for in vitro proliferation of dendritic cell precursors and their use to produce immunogens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MAFFEI A. ET AL.: "A novel closed system utilizing styrene copolymer bead adherence for the production of human dendritic cells", TRANSFUSION, vol. 40, November 2000 (2000-11-01), pages 1419 - 1420, XP002961483 *

Also Published As

Publication number Publication date
AU2002326463A1 (en) 2003-02-17
WO2003010292A2 (en) 2003-02-06

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