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WO2003010185A3 - Method for making acylated polypeptides - Google Patents

Method for making acylated polypeptides Download PDF

Info

Publication number
WO2003010185A3
WO2003010185A3 PCT/DK2002/000501 DK0200501W WO03010185A3 WO 2003010185 A3 WO2003010185 A3 WO 2003010185A3 DK 0200501 W DK0200501 W DK 0200501W WO 03010185 A3 WO03010185 A3 WO 03010185A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
certain
lysine
host cells
transformed host
Prior art date
Application number
PCT/DK2002/000501
Other languages
French (fr)
Other versions
WO2003010185A2 (en
Inventor
Per Balschmidt
Ivan Diers
Michi Egel-Mitani
Jan Markussen
Thomas Hoeeg-Jensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Priority to EP02750841A priority Critical patent/EP1421102A2/en
Priority to JP2003515544A priority patent/JP2005503141A/en
Priority to AU2002355160A priority patent/AU2002355160A1/en
Publication of WO2003010185A2 publication Critical patent/WO2003010185A2/en
Publication of WO2003010185A3 publication Critical patent/WO2003010185A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/605Glucagons

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention is related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which is to be acylated in a subsequent in vitro step. The precursor molecule comprises an N-terminal extension has a cleavage site different from lysine for cleavage from the desired polypeptide. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ϵ-amino groups. Specifically, glucagon like protein-1 (GLP-1(7-37)) is used.
PCT/DK2002/000501 2001-07-24 2002-07-18 Method for making acylated polypeptides WO2003010185A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02750841A EP1421102A2 (en) 2001-07-24 2002-07-18 Method for making acylated polypeptides
JP2003515544A JP2005503141A (en) 2001-07-24 2002-07-18 Methods for making acylated polypeptides
AU2002355160A AU2002355160A1 (en) 2001-07-24 2002-07-18 Method for making acylated polypeptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101140 2001-07-24
DKPA200101140 2001-07-24

Publications (2)

Publication Number Publication Date
WO2003010185A2 WO2003010185A2 (en) 2003-02-06
WO2003010185A3 true WO2003010185A3 (en) 2004-03-25

Family

ID=8160636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000501 WO2003010185A2 (en) 2001-07-24 2002-07-18 Method for making acylated polypeptides

Country Status (4)

Country Link
EP (1) EP1421102A2 (en)
JP (1) JP2005503141A (en)
AU (1) AU2002355160A1 (en)
WO (1) WO2003010185A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006108270A1 (en) 2005-04-11 2006-10-19 Pharmagap Inc. Inhibitors of protein kinases and uses thereof
CA2659958A1 (en) * 2005-08-05 2007-02-15 Pharmagap Inc. Peptides targeted to protein kinase c isoforms and uses thereof
EP1872790A1 (en) 2006-06-26 2008-01-02 DeveloGen Aktiengesellschaft New formulation for increasing bioavailability of neurturin
US20100317057A1 (en) 2007-12-28 2010-12-16 Novo Nordisk A/S Semi-recombinant preparation of glp-1 analogues

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995035384A1 (en) * 1994-06-17 1995-12-28 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
EP0712862A2 (en) * 1994-11-17 1996-05-22 Eli Lilly And Company Selective acylation of epsilon-amino groups
US5905140A (en) * 1996-07-11 1999-05-18 Novo Nordisk A/S, Novo Alle Selective acylation method
US6011007A (en) * 1993-09-17 2000-01-04 Novo Nordisk A/S Acylated insulin
WO2000055119A1 (en) * 1999-03-17 2000-09-21 Novo Nordisk A/S Method for acylating peptides and novel acylating agents

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6011007A (en) * 1993-09-17 2000-01-04 Novo Nordisk A/S Acylated insulin
WO1995035384A1 (en) * 1994-06-17 1995-12-28 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
EP0712862A2 (en) * 1994-11-17 1996-05-22 Eli Lilly And Company Selective acylation of epsilon-amino groups
US5905140A (en) * 1996-07-11 1999-05-18 Novo Nordisk A/S, Novo Alle Selective acylation method
WO2000055119A1 (en) * 1999-03-17 2000-09-21 Novo Nordisk A/S Method for acylating peptides and novel acylating agents

Also Published As

Publication number Publication date
AU2002355160A1 (en) 2003-02-17
JP2005503141A (en) 2005-02-03
WO2003010185A2 (en) 2003-02-06
EP1421102A2 (en) 2004-05-26

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