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WO2003008979A3 - Methode - Google Patents

Methode Download PDF

Info

Publication number
WO2003008979A3
WO2003008979A3 PCT/GB2002/003299 GB0203299W WO03008979A3 WO 2003008979 A3 WO2003008979 A3 WO 2003008979A3 GB 0203299 W GB0203299 W GB 0203299W WO 03008979 A3 WO03008979 A3 WO 03008979A3
Authority
WO
WIPO (PCT)
Prior art keywords
prion protein
nuclease
purify
sample
prion
Prior art date
Application number
PCT/GB2002/003299
Other languages
English (en)
Other versions
WO2003008979A2 (fr
Inventor
Jonathan D F Wadsworth
John Collinge
Original Assignee
Medical Res Council
Jonathan D F Wadsworth
John Collinge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Jonathan D F Wadsworth, John Collinge filed Critical Medical Res Council
Priority to AU2002317969A priority Critical patent/AU2002317969A1/en
Publication of WO2003008979A2 publication Critical patent/WO2003008979A2/fr
Publication of WO2003008979A3 publication Critical patent/WO2003008979A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2828—Prion diseases

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne des méthodes servant à préparer la protéine du prion et consistant à produire un échantillon d'homogénat, à mettre en contact l'échantillon avec une nucléase, à faire incuber l'échantillon, et à recueillir la protéine du prion. On recueille, de préférence, le prion par précipitation, telle qu'une précipitation d'acide phosphotungstique. Cette invention a également trait à une protéine du prion préparée selon de telles méthodes.
PCT/GB2002/003299 2001-07-19 2002-07-18 Methode WO2003008979A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002317969A AU2002317969A1 (en) 2001-07-19 2002-07-18 Method to purify prion protein using nuclease

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0117608A GB0117608D0 (en) 2001-07-19 2001-07-19 Method
GB0117608.0 2001-07-19

Publications (2)

Publication Number Publication Date
WO2003008979A2 WO2003008979A2 (fr) 2003-01-30
WO2003008979A3 true WO2003008979A3 (fr) 2003-05-30

Family

ID=9918802

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/003299 WO2003008979A2 (fr) 2001-07-19 2002-07-18 Methode

Country Status (3)

Country Link
AU (1) AU2002317969A1 (fr)
GB (1) GB0117608D0 (fr)
WO (1) WO2003008979A2 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042487A1 (fr) * 1998-02-20 1999-08-26 The Regents Of The University Of California Technique permettant de concentrer une proteine a conformation liee a une affection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042487A1 (fr) * 1998-02-20 1999-08-26 The Regents Of The University Of California Technique permettant de concentrer une proteine a conformation liee a une affection

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF VIROLOGY (1990), 112(3-4), 215-29, 1990 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; KELLINGS, K. ET AL: "Nucleic acids in prion preparations: unspecific background or essential component?", XP002230961, retrieved from STN Database accession no. 122:154313 CA *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; SASAKI, KAZUHIKO ET AL: "Biological activity of subfractions from scrapie-associated fibrils", XP002230962, retrieved from STN Database accession no. 117:45704 CA *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; SKLAVIADIS, T. ET AL: "Nuclease treatment results in high specific purification of Creutzfeldt-Jakob disease infectivity with a density characteristic of nucleic acid-protein complexes", XP002230963, retrieved from STN Database accession no. 114:119646 CA *
J SAFAR ET AL.: "Eight prion strains have PrPsc molecules with different conformations", NATURE MEDICINE., vol. 4, no. 10, October 1998 (1998-10-01), NATURE PUBLISHING, CO., US, pages 1157 - 1165, XP002230960, ISSN: 1078-8956 *
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON, SERIES B: BIOLOGICAL SCIENCES (1994), 343(1306), 425-30, 1994 *
S B PRUSINER ET AL.: "Further purification and characterization of scrapie prions", BIOCHEMISTRY., vol. 21, no. 26, 1982, AMERICAN CHEMICAL SOCIETY. EASTON, PA., US, pages 6942 - 6950, XP002230959, ISSN: 0006-2960 *
VIRUS RESEARCH (1992), 23(3), 241-51, 1992 *

Also Published As

Publication number Publication date
WO2003008979A2 (fr) 2003-01-30
GB0117608D0 (en) 2001-09-12
AU2002317969A1 (en) 2003-03-03

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