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WO2003006947A3 - Methode et composition de criblage cellulaire - Google Patents

Methode et composition de criblage cellulaire Download PDF

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Publication number
WO2003006947A3
WO2003006947A3 PCT/US2002/021339 US0221339W WO03006947A3 WO 2003006947 A3 WO2003006947 A3 WO 2003006947A3 US 0221339 W US0221339 W US 0221339W WO 03006947 A3 WO03006947 A3 WO 03006947A3
Authority
WO
WIPO (PCT)
Prior art keywords
transcription
methods
reporter enzyme
degradation
reporter
Prior art date
Application number
PCT/US2002/021339
Other languages
English (en)
Other versions
WO2003006947A2 (fr
Inventor
Sharat Singh
Po-Ying Chan-Hui
Original Assignee
Aclara Biosciences Inc
Sharat Singh
Po-Ying Chan-Hui
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc, Sharat Singh, Po-Ying Chan-Hui filed Critical Aclara Biosciences Inc
Priority to AU2002316577A priority Critical patent/AU2002316577A1/en
Publication of WO2003006947A2 publication Critical patent/WO2003006947A2/fr
Publication of WO2003006947A3 publication Critical patent/WO2003006947A3/fr
Priority to US10/740,079 priority patent/US20040175765A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de criblage cellulaire multiplexé. On peut mettre en application ces méthodes afin de quantifier simultanément les niveaux de transcription de promoteurs ou de médicaments multiples ou de contrôler les effets exercés sur des interactions multiples protéine-protéine. Ces méthodes utilisent des produits de recombinaison de gènes rapporteurs couplant une seule séquence codante d'enzyme rapporteur à une ou plusieurs régions du promoteur, ainsi qu'un ensemble de sondes soumises à une dégradation par l'enzyme rapporteur, ce qui produit un ensemble de rapporteurs pouvant être séparés les uns des autres par leurs différences de mobilité. On exécute des essais multiplexés par combinaison d'un ensemble de population cellulaire, chaque population contenant un produit de recombinaison distinct de gène rapporteur et une sonde distincte. On exécute ensuite différents traitements sur le mélange de cellules. Les traitements induisant la transcription de promoteurs spécifiques provoqueront la synthèse de l'enzyme rapporteur dans une population cellulaire déterminée, ce qui conduit à la dégradation de la sonde correspondante. On détermine la génération des rapporteurs spécifiques par électrophorèse ou d'autres moyens de mesure de la mobilité et on la met en corrélation avec un effet du traitement sur la transcription d'un promoteur défini.
PCT/US2002/021339 2001-07-09 2002-07-05 Methode et composition de criblage cellulaire WO2003006947A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002316577A AU2002316577A1 (en) 2001-07-09 2002-07-05 Cell-screening assay and composition
US10/740,079 US20040175765A1 (en) 2002-07-05 2003-12-18 Cell-screening assay and composition

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30429601P 2001-07-09 2001-07-09
US60/304,296 2001-07-09
US32529201P 2001-09-27 2001-09-27
US60/325,292 2001-09-27

Publications (2)

Publication Number Publication Date
WO2003006947A2 WO2003006947A2 (fr) 2003-01-23
WO2003006947A3 true WO2003006947A3 (fr) 2003-05-08

Family

ID=26973933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021339 WO2003006947A2 (fr) 2001-07-09 2002-07-05 Methode et composition de criblage cellulaire

Country Status (2)

Country Link
AU (1) AU2002316577A1 (fr)
WO (1) WO2003006947A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523314A (ja) 2003-04-01 2006-10-12 モノグラム バイオサイエンシズ，インコーポレーテッド バイオマーカーとしてのＥｒｂＢ表面受容体複合体
WO2009086197A1 (fr) 2007-12-20 2009-07-09 Monogram Biosciences, Inc. Procédés de diagnostic du her-2
EP2438442B1 (fr) 2008-12-01 2017-08-09 Laboratory Corporation of America Holdings Procédés et dosages pour mesurer p95 et/ou des complexes de p95 dans un échantillon et anticorps spécifiques de p95
US10724099B2 (en) 2012-03-16 2020-07-28 The Broad Institute, Inc. Multiplex methods to assay mixed cell populations simultaneously
EP2904390B1 (fr) 2012-10-02 2016-11-16 Roche Diagnostics GmbH Procédés de libération spécifique d'un sous-groupe d'objets
JP2019515252A (ja) 2016-03-15 2019-06-06 ラボラトリー コーポレイション オブ アメリカ ホールディングス 細胞間のタンパク質相互作用を評価する方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725535A (en) * 1983-02-22 1988-02-16 Sonenshein Abraham L Promoter probe vectors
US5658736A (en) * 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725535A (en) * 1983-02-22 1988-02-16 Sonenshein Abraham L Promoter probe vectors
US5658736A (en) * 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE44437E1 (en) 2003-04-01 2013-08-13 Monogram Biosciences, Inc. Methods for detecting receptor complexes comprising PI3K
US9110066B2 (en) 2009-01-15 2015-08-18 Laboratory Corporation Of America Holdings HER-3 antibodies and methods of use

Also Published As

Publication number Publication date
AU2002316577A1 (en) 2003-01-29
WO2003006947A2 (fr) 2003-01-23

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