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WO2003005810A3 - Cloned non-human mammals from contact inhibited donor cells - Google Patents

Cloned non-human mammals from contact inhibited donor cells Download PDF

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Publication number
WO2003005810A3
WO2003005810A3 PCT/US2002/021680 US0221680W WO03005810A3 WO 2003005810 A3 WO2003005810 A3 WO 2003005810A3 US 0221680 W US0221680 W US 0221680W WO 03005810 A3 WO03005810 A3 WO 03005810A3
Authority
WO
WIPO (PCT)
Prior art keywords
nuclear transfer
donor cells
human mammals
transfer unit
cloned non
Prior art date
Application number
PCT/US2002/021680
Other languages
French (fr)
Other versions
WO2003005810A2 (en
WO2003005810A9 (en
Inventor
William Fodor
Jagdeece Rampsoondar
Kenneth R Bondioli
Original Assignee
Alexion Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc filed Critical Alexion Pharma Inc
Priority to AU2002326353A priority Critical patent/AU2002326353A1/en
Publication of WO2003005810A2 publication Critical patent/WO2003005810A2/en
Publication of WO2003005810A3 publication Critical patent/WO2003005810A3/en
Publication of WO2003005810A9 publication Critical patent/WO2003005810A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cloned non-human mammalian nuclear transfer units, embryos and animals are provided by introducing a contact-inhibited non-cycling mammalian donor cell in the G1 state or a nucleus thereof into an enucleated oocyte from a non-human mammal of the same species as the donor to form a nuclear transfer unit, implanting the nuclear transfer unit into the uterus of a surrogate mother, permitting the nuclear transfer unit to develop into the cloned non-human mammalian embryo and allowing the embryo to come to term.
PCT/US2002/021680 2001-07-09 2002-07-09 Cloned non-human mammals from contact inhibited donor cells WO2003005810A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002326353A AU2002326353A1 (en) 2001-07-09 2002-07-09 Cloned non-human mammals from contact inhibited donor cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30443101P 2001-07-09 2001-07-09
US60/304,431 2001-07-09

Publications (3)

Publication Number Publication Date
WO2003005810A2 WO2003005810A2 (en) 2003-01-23
WO2003005810A3 true WO2003005810A3 (en) 2003-08-14
WO2003005810A9 WO2003005810A9 (en) 2004-02-26

Family

ID=23176483

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021680 WO2003005810A2 (en) 2001-07-09 2002-07-09 Cloned non-human mammals from contact inhibited donor cells

Country Status (3)

Country Link
US (1) US20030101469A1 (en)
AU (1) AU2002326353A1 (en)
WO (1) WO2003005810A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2909775A1 (en) 2005-08-09 2007-03-29 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
WO2012112586A1 (en) 2011-02-14 2012-08-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
JP2023551404A (en) 2020-11-20 2023-12-08 レビビコア， インコーポレイテッド Multiple transgenic pigs with growth hormone receptor knockout for cross-species transplantation
EP4404740A1 (en) 2021-09-20 2024-07-31 Revivicor Inc. Multitransgenic pigs comprising ten genetic modifications for xenotransplantation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153428A (en) * 1994-04-13 2000-11-28 Biotransplant, Inc. α(1,3) galactosyltransferase negative porcine cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6107543A (en) * 1992-08-20 2000-08-22 Infigen, Inc. Culture of totipotent embryonic inner cells mass cells and production of bovine animals
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6215041B1 (en) * 1997-01-10 2001-04-10 University Of Mmassachusetts Cloning using donor nuclei from a non-quiesecent somatic cells
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
US6258998B1 (en) * 1998-11-24 2001-07-10 Infigen, Inc. Method of cloning porcine animals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153428A (en) * 1994-04-13 2000-11-28 Biotransplant, Inc. α(1,3) galactosyltransferase negative porcine cells
US6413769B1 (en) * 1994-04-13 2002-07-02 University Of London α(1,3) galactosyltransferase negative porcine cells
US20030014770A1 (en) * 1994-04-13 2003-01-16 The General Hospital Corporation Alpha (1,3) galactosyl transferase negative swine

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHONG T. ET AL.: "Anti-galactose-alpha(1,3) galactose antibody production in a 1,3-galactosyltransferase gene knockout mice after xeno and allo transplantation", TRANSPLANT IMMUNOLOGY, vol. 8, no. 2, June 2000 (2000-06-01), pages 129 - 137, XP002964050 *
DAI ET AL.: "Targeted disruption of the a1 1.3 galactosyltransferase gene in cloned pigs", NATURE BIOTECHNOLOGY, vol. 20, March 2002 (2002-03-01), pages 251 - 255, XP002962716 *
LIN ET AL.: "The role of antibodies in acute vascular rejection of pig-to-baboon cardiac transplants", JOURNAL OF CLINICAL INVESTIGATIONS, vol. 101, no. 8, April 1998 (1998-04-01), pages 1745 - 1756, XP002964051 *
VANHOVE ET AL.: "Genetic engineering in the pig", ANN. N.Y. ACAD. SCI., vol. 862, 30 December 1998 (1998-12-30), pages 28 - 36, XP000906935 *

Also Published As

Publication number Publication date
WO2003005810A2 (en) 2003-01-23
US20030101469A1 (en) 2003-05-29
WO2003005810A9 (en) 2004-02-26
AU2002326353A1 (en) 2003-01-29

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